Iris setosa var.
hondoensis and
I. setosa var.
nasuensis are endemic to Japan, and their origins still remain to be clarified. In this study, we report the characterization of interspecific hybrids (allotriploids) from the cross between autotetraploid
I. setosa var.
setosa (4
x) and diploid
I. laevigata (2
n=32) and their relationship to
I. setosa var.
hondoensis or
I. setosa var.
nasuensis. In reciprocal crosses between
I. setosa var.
setosa (2
x, 4
x) and
I. laevigata, progeny plants were obtained only in the cross between
I. setosa var.
setosa (4
x) and
I. laevigata (2
x). These progenies were identified as allotriploids (2
n=54) and their aneuploids (2
n=53) of
I. setosa var.
setosa (4
x)×
I. laevigata (2
x) by flow cytometric (FCM), cytological and random amplified polymorphic DNA (RAPD) analyses, and the five hybrids (SL1–5) except SL6 were characterized. Furthermore, these characteristics of
I. setosa var.
hondoensis and
I. setosa var.
nasuensis were compared with those of SL1–5. These varieties were also examined by FCM, cytological, RAPD and cleaved amplified polymorphic sequence (CAPS) analyses, which comparative analyses showed that varieties have the hybrid nature between
I. setosa var.
setosa and
I. laevigata, and maternal plants are organelle DNAs of the former species.
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