CYTOLOGIA
Online ISSN : 1348-7019
Print ISSN : 0011-4545
Volume 50, Issue 3
Displaying 1-27 of 27 articles from this issue
  • Ira Sinha
    1985 Volume 50 Issue 3 Pages 417-425
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
    JOURNAL FREE ACCESS
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  • I. Effect of vinblastine and yohimbine on meiosis of Vicia faba
    Amal S. Shehab
    1985 Volume 50 Issue 3 Pages 427-431
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    In this investigation the effect of vinblastine and yohimbine alkaloids on Vicia faba PMCs was studied. The two alkaloids gave high percentage of abnormalities. The effect of the alkaloids was temporary. They induced different chromosomal aberrations, as stickiness, bridges, spindle disturbance, lagging, breaks and despiralization. Breaks were more evident in vinblastine. Despiralization was recorded in yohimbine only.
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  • R. Sai Kumar, U. P. Singh, R. M. Singh, R. B. Singh
    1985 Volume 50 Issue 3 Pages 433-443
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    Nineteen tertiary trisomics were isolated from some translocation heterozygotes and interchange trisomics of pearl millet. Cytological analysis of these trisomics indicates that chromosome association of trivalents, univalents and pentavalents were frequent in all the trisomics. But their ratio varied from one trisomic to the next. Other associations were relatively infrequent. The relative frequencies of 6 pentavalent configurations observed in different trisomics were studied and their probable association with mode of fertility and transmission rates have been discussed. Furthermore, the techniques for isolation of balanced tertiary trisomics and their further use in production of hybrid seeds in pearl millet have been discussed.
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  • N. Omanakumari, P. M. Mathew
    1985 Volume 50 Issue 3 Pages 445-451
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    Detailed karyomorphology of four species of Zingiber from South India, has been studied, namely Z. officinale (2n=22), Z. zerumbet (2n=22), Z. wightianum (2n=22) and Z. macrostachyum (2n=22). Karyomorphological data indicate that except Z. officinale, where it is relatively symmetrical (1A), all the other three species are moderately asymmetrical (2A).
    Chromosomal evolution on the genus is discussed in the light of available cytological findings. The data show that this is a monobasic genus with x=11. Although the karyotypes of the four species showed general uniformity, in final details recognisable differences were noticed with regard to distribution of secondary constrictions and centromere positions of a few individual chromosomes. It is evident that numerical and gross structural changes in chromosomes have not played any maior role in si eciation and evolution of the genus.
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  • K. V. Bhavanandan
    1985 Volume 50 Issue 3 Pages 453-459
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    Detailed cytological study of B. quoyana (2n=123) was made. Regular formation of 41 bivalents and 41 univalents was consistantly noticed in the spore mother cells.
    Detailed karyomorphology of the material was studied and the data indicated that the karyotype is composed of two distinct sets, a diploid one consisting of 41 pairs, and a haploid set consisting of 41 non-homologous chromosomes.
    The result appeared to indicate that this is an allotriploid species possibly originated spontaneously by the fusion between the unreduced (2n) and normal (n) gametes of two diploid species of Bolbitis.
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  • Jean Dexheimer, Christine Marx, Vivienne Gianinazzi-Pearson, Silvjo Gi ...
    1985 Volume 50 Issue 3 Pages 461-471
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    Various membranous configurations (vesicles, tubules, membrane layers) produced by the host and fungus have been studied in five different vesicular-arbuscular (VA) mycorrhizae using electron microscope techniques. The structural, cytochemical and enzymological aspects of these membrane formations show that they are produced by the plasmalemma and correspond to plasmalemmasomes. The plasmalemmasomes in the VA fungal hyphae appear to originate by invagination of the fungal plasmalemma whilst those of the host cell form by extrusion of the host membrane into the interfacial matrix. The presence of plasmalemmasomes in one of the mycorrhizal symbionts does not necessarily correspond to their presence in the other. (The possible role of these plasmalemma formations in VA mycorrhizal associations is discussed).

    Plusieurs configurations membranaires (vésicules, tubules, systèmes membranaires concentriques) produites par I'hôte et le champignon ont été étudiées en microscopie électronique chez cinq associations mycorhiziennes vésiculo-arbusculaires (VA) différentes. Les aspects structuraux, cytochimiques et enzymologiques de ces formations membranaires montrent qu'elles sont produites par le plasmalemme et qu'elles correspondent aux plasmalemmasomes.
    Les plasmalemmasomes dans les hyphes du champignon VA paraissent prendre naissance de l'invagination du plasmalemme fongique tandis que ceux de la cellule-hôte se forment par évagination de la membrane de l'hôte dans la matrice de l'interface. Toutefois, on ne trouve pas nécessairement ces formations simultanément dans les deux symbiotes. Le rôle éventuel de ces structures plasmalemmiques dans les associations mycorhiziennes est discuté.
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  • T. Govindarajan, D. Subramanian
    1985 Volume 50 Issue 3 Pages 473-482
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    Karyomorphological details pertaining to chromosome measurements in 23 taxa of South Indian Acanthaceae have been described. Chromosome numbers determined in the present study ranges from 2n=18 to 2n=80. Karyotypes in the family also show differences in absolute chromosome length indicating changes in nuclear DNA in evolution. No definite trend of either phylogentic increase or dercrease of chromosome size is indicated by the available data.
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  • M. L. Sharma
    1985 Volume 50 Issue 3 Pages 483-486
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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  • M. Sanjappa, R. P. Bhatt
    1985 Volume 50 Issue 3 Pages 487-497
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    The present paper deals with the chromosome numbers and morphology of thirteen species of the genus Desmodium. Twelve out of thirteen species show 2n=22, while one species (D. salicifolium) shows 2n=20. A review of present and previous chromosome number reports justifies the basic numbers x=11 and 10 for the genus. The karyotype analysis is made for the first time in ten species. In all the species investigated here only nearly median and nearly submediam chromosomes are observed. On the basis of karyomorphology D. salicifolium is relatively primitive compared to D. gangeticum, while D. laxiflorum, D. uncinatum, D. triflorum, D. sandwiscence, D. velutinum, D. distortum, D. ritchiei, D. heterocarpon var. strigosum, D. dichotomum, D. elegans and D. intortum occupy the intermediate position. The interrelationship among the species is reticulate. The minor differences between the karyotypes of different species indicate that structural alterations have played an important role in speciation. Karyornorphological observations are discussed in light of Ohashi's infrageneric classification.
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  • T. V. V. Seetharami Reddi, V. R. Reddi
    1985 Volume 50 Issue 3 Pages 499-505
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    Dry, well filled seeds of rice varieties T(N)1, IR 8 and Sona were treated with chemical mutagens: methyl methane sulphonate (MMS), dimethyl sulphate (DMS), dichloroethyl methane suiphonate (DEMS) and diethyl sulphate (dES) with a view to study their effects in inducing chromosomal aberrations. DEMS showed highest aberration rate (29.6%) followed by DMS (27.7%), dES (17.7%) and MMS (7.5%). The frequency of aberrations induced in Sona was relatively more (24.5%) compared to T (N) 1 (23.1%) and IR 8 (6.3%).
    The most common type of abnormality noticed in the meiotic cells involved the nucleolus. Other abnormalities induced include cryptic alterations in the pachytene chromosomes, delayed separation and bridges and fragments at anaphase I and higher incidence of rod bivalents and univalents.
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  • 3. The Karyotype of Paraechinus aethiopicus
    A. A. Al-Saleh, M. A. Khan
    1985 Volume 50 Issue 3 Pages 507-512
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    The karyotype of Paraechinus aethiopicus consists of 48 chromosomes comprising 14 pairs of metacentric, 9 pairs of submetacentric and a pair of metacentric sex chromosomes, X and Y. The fundamental number is 96 and the fundamental number of autosmes is 92. Secondary constrictions and subtelocentric chromosomes are not seen in this animal.
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  • N. Vijayakumar, D. Subramanian
    1985 Volume 50 Issue 3 Pages 513-520
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    Karyotypic analyses were made using root tip mitosis in 10 collections representing 8 species of South Indian Myrtaceae. First records of chromosome numbers have been made in Rhodomyrtus tomentosa (2n=22 and n=11), Syzygium calophyllifolium (2n=22), S. jambolanum (2n=22 and n=11), S. montanum (n=11), Eugenia caryophyllata (2n=22) and Eucalyptus torelliana (n=11). Meiotic studies were made in 10 species. Eventhough all the species mitotically studied possess 22 chromosomes as diploid number, karyotype alterations together with polyploidy play important role in speciation in Myrtaceae. The species meiotically studied show 11 bivalents in PMCs as haploid number.
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  • Effect of the insecticide Methamidophos on root-mitosis of Vicia faba
    Soheir M. Amer, Odette R. Farah
    1985 Volume 50 Issue 3 Pages 521-526
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    The effect of the insecticide O, S-dimethyl phosphoramidothioate (Methamidophos) has been studied on root-mitosis of Vicia faba. Two types of treatments were conducted: seed-soak-treatment for 24 hours and root-treatment for 4 hours. Different concentrations of the insecticide ranging from 500 ppm to 31.25 ppm have been used in each treatment.
    The insecticide showed no significant effect on the mitotic index, meanwhile it induced a highly significant percentage of cells with chromosome aberrations after both seed-soak- and root-treatments. Roots treated with 500 ppm of Methamidophos recovered after replacement in water for 48 hours.
    Disturbed meta- and anaphases where the chromosomes spread irregularly over the cell dominated. Other abnormalities e.g. chromosome stickiness, lagging chromosomes and chromosome fragmentation were observed.
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  • Suad Artvinli, Esin Asan
    1985 Volume 50 Issue 3 Pages 527-533
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    To support the view that an eucaryotic cell is not compartmentalized but is an integrated membranous structure, the skeletal muscle cell was chosen and the ultrastructure was examined. The tissue was prepared by using the method which demonstrates the network constituting the cell. None of the known membranous substructures was observed within the muscle cell except nucleus. Even the myofibrils showing clear striations had unusual appearance. All the structures present were integrated to form the muscle cell.
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  • Animesh K. Datta, Amal K. Biswas
    1985 Volume 50 Issue 3 Pages 535-543
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    A busyy mutant with desynaptic behaviour of chromosomes has been recovered from the M2 population of Nigella sativa L. (black cummin) following 2 hours treatment with 0.5% EMS. A single pair of recessive genes (bu/bu) has been ascribed for bushyness and the mutant bred true in the subsequent generations. The bushy mutant plant could always be characterized by their delayed germination, flowering and maturity, high frequency of sterile pollen, poor seed setting and desynaptic behaviour of chromosomes. Possibly, the mutant gene has shown pleiotropic effect or the traits were closely linked with the bushy phenotype.
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  • Animesh K. Datta, Amal K. Biswas
    1985 Volume 50 Issue 3 Pages 545-562
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    For induction of mutations, seeds (1.5% moisture content) of Nigella sativa L. (black cummin) were treated with 4. 6, 8, 10, 20 and 30 KR of X-rays and 0.5%, 0.75% and 1.0% EMS for 2 and 4 hours. Seed germinability, survival rates, LD50, the M1 variants, the total mutation frequency including chlorophyll mutations, viable mutations and non-viable morphological mutations have been enumerated. Mutagenic efficiency and effectiveness were determined. Mode of inheritance of some of the mutant traits was also studied.
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  • R. Herich, A. Lux
    1985 Volume 50 Issue 3 Pages 563-569
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    Ubisch bodies observed on the surface of tapetal cells in the anthers of Lilium henryi, are composed by the central electron-transparent part (core or nucleus) and by the marginal electron-dense zone. The central part is connected by a canal with the plasma membrane of the tapetal cells. ER participates at Ubisch bodies formation, it gathers electron-dense material, which causes their dilatation. During the formation of the electron-transparent nucleus of the Ubisch body, it is possible to observe a membrane on the surface of it. Clusters of dense material are apposed gradually on the surface of the nucleus, forming the dark marginal zone of the body. The Ubisch bodies are transported to the surface of the tapetal cells by the canals, they are connected with the plasma membrane and become lytically active. At the contact of the Ubisch bodies with the plasma membrane, lysis occurs of the surface parts of the cells. Basing on these observations, we consider Ubisch bodies to be a part of the lytic compartment of the tapetal cells. Differentiation of the Ubisch bodies and their lytic activity are simultaneously accompanied by intensive vacuolation of the tapetal cells, indicating relations of these two accurately programed processes.
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  • K. Khandakar, S. Arangzeb, M. Husain
    1985 Volume 50 Issue 3 Pages 571-575
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    The maximum number of nucleoli organized at early telophase and that of secondary or satellited chromosomes attached to the fusion nucleolus at late prophase stage was studied in Savar- D-154 and D-154 (C.V.) of C. capsularis, O-4 (C.V.) of C. olitorius and a wild species, C. trilocularis. Savar- D-154 of C. capsularis and the wild species C. trilocularis have 6 and 4 nucleoli respectively in early telophase stage and 6 and 4 attached chromosomes to the fusion nucleolus at late prophase stage. The two varieties D-154 and 0-4 organized 5 nucleoli at early telophase stage but 6 attached chromosomes to the fusion nucleolus at late prophase stage. Absence of one nucleolus may be due to the failure of one secondary constricted chromosomes in organizing a nucleolus. This study showed a correlation between the organization of maximum number of nucleoli at early telophase stage and that of satellited or attached chromosomes to the fusion nucleolus at late prophase stage.
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  • A. A. Barrion, R. C. Saxena
    1985 Volume 50 Issue 3 Pages 577-582
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    The cytology of a grass-infesting planthopper, N. bakeri, was studied. The genomic complement was normally 2n=29 in males and 2n=30 chromosomes in females. These specifically consisted of 28 autosomes (1411) and a univalent X-chromosome in males and XX bivalents in females.
    As attested by the orientation, broad spindle attachment, and parallel disjunction during anaphase I, N. bakeri chromosomes are holocentric or the kinetochores are located along the entire length of the chromosomes.
    Some karyological variations included meiocytes with less as well as more chromosomes than the normal genomic constitution. Due to these atypical karyotypes, it can be suggested that N. bakeri is chromosomally polymorphic with ample genetic versatility.
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  • G. Viji Stella Bai, D. Subramanian
    1985 Volume 50 Issue 3 Pages 583-592
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    Cytotaxonomical investigation has been made in 21 species of Piper and Peperomia, of which first records of chromosome numbers have been made in Piper attenuatum, P. galeatum, P. schmidtii, P. wightii, P. brachystachum, P. hookeri, Peperomia marmorata and P. rubella.
    11 is the basic number for Peperomia and 12 for Piper as evidenced by the present investigations. The species of Piper are higher polyploids and aneuploids and the species of Peperomia are mostly aneuploids. The karyotype analyses show that both genera are characterised by the presence of mostly submetacentric and telocentric chromosomes showing thereby that the two genera are highly evolved but in different lines, eventhough, they might have been originated from a common ancestor.
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  • Vasilis K. Dimitriadis
    1985 Volume 50 Issue 3 Pages 593-601
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    Short-time starvation (until 2 hrs) of Drosophila auraria larvae at the early third or mid-third instar affected the fine structure of the gastric caeca epithelial cells but had no effect on the cells of the anterior, middle and posterior midgut regions. In gastric caeca cells starvation produces: (1) an abrupt increase in the number of secretory granules, (2) dilation of the infoldings of basal plasma membrane, (3) decrease in the number of the openings by which the haemolymph communicates with these cells. Refeeding of the starved larvae reverses all these changes.
    After the 2 hrs starvation period, the number of the secretory granules produced by the gastric caeca and by the midgut cells decreased gradually. About 3-4 hrs of starvation the number of the secretory granules was less than that found in “normal fed larvae”, but the cells continued the production of secretory granules until larval pupation.
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  • C. Singh, A. P. Shamsherry
    1985 Volume 50 Issue 3 Pages 603-610
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    Effects of concentration, treatment temperature and hydrogen ion concentration on biological activity of 1, 3-Propane sultone (PS) were studied in barley (Hordeum vulgare). Physiological as well as genetic effects of mutagen showed a linear increase with increase in the concentration of mutagen and its treatment temperature. Higher mutagenic effectiveness and efficiency of PS was recorded following treatment with 6 mM and at 25°C. The effect of pH was apparent on root growth injury, as compared to seedling growth damage. A higher mutagenic efficiency was however, recorded at pH=4.6.
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  • Yasuo Hotta, Alicia de la Peña, Satoshi Tabata, Herbert Stern
    1985 Volume 50 Issue 3 Pages 611-620
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    Localized changes in chromatin structure occur during the premeiotic and meiotic prophase intervals of Lilium microsporocytes. The changes cause the chromatin regions affected to become more accessible to the actions of DNase I and II, and of micrococcal nuclease. Each type of change is associated with a specific meiotic process and involves specific groups of DNA sequences. The processes thus far identified with such changes are DNA replication at premeiosis, zygotene, and pachytene, and DNA transcription of meiosis-specific messages. The relationship of structural changes in chromatin to the regulation of meiotic DNA metabolism is discussed.
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  • G. K. Manna, N. Ueshima, S. K. Dey, S. Deb-Mallick
    1985 Volume 50 Issue 3 Pages 621-630
    Published: September 25, 1985
    Released on J-STAGE: March 19, 2009
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    Testes of Physopelta schlanbuschi, P. quadrigutta and P. gutta studied from India contained 2n=17, 12A+2m+X1X2Y chromosomes in spermatogonial metaphase and 6 autosomal bivalents, two m chromosomes and 3 univalent sex chromosomes (X1X2Y) during prophase I and metaphase I. Anaphae I was reductional for the autosomes and equational for the sex chromosomes and at metaphase II sex chromosomes formed a pseudotrivalent structure located in the mid region and the autosomes formed a ring like arrangement in the equatorial region of the hollow spindle. Anaphase II was reductional for the sex chromosomes, for X1 and X2 moved to one pole and the Y to the opposite pole. The X1 and the Y were morphologically indistinguishable and formed two conspicuously large markers with some difference in their size in the 3 species among which P. gutta had relatively small X1 and Y. The Japanese population of P. gutta had the same chromosome constitution like that of the Indian population but the relative size of the X1 and Y in two populations varied and in Japanese population they were not readily demarcable from 12 small autosomes in spermatogonial plates. Males of another species, P. cincticollis also studied from Japan had 2n=15, 12A+2m+XO in spermatogonial complement and 6 autosomal bivalents, two m chromosomes and the single X at prophase I and metaphase I while 6 autosomes, one m chromosome and the X at the metaphase II. Anaphase II was reductional for the sex chromosome, the X moved to only one pole with 6 autosomes and one m while the opposite pole was without the X. Thus species of Physopelta studied from India had the uniform diploid number, sex chromosome mechanism and the meiotic behaviour of chromosomes while those of Japan had two types of diploid numbers, 15 in XO species and 17 in X1X2Y species. Thus Japanese species of Physopelta were chromosomally more variable showing intra- and interspecific sex chromosomal variations.
    The presence of Y chromosome in 3 out of 4 species of Physopelta happened to be exceptional as the same has not been recorded in any other species in Largidae and in its allied family Pyrrhocoridae so far. The occurrence of only X1X2Y type males in India might have selective advantage and different tentative suggestions have been put forth to account for the origin of the diversified sex chromoso memechanism in different species of Physopelta.
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  • 1985 Volume 50 Issue 3 Pages e1a
    Published: 1985
    Released on J-STAGE: March 19, 2009
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  • 1985 Volume 50 Issue 3 Pages e1b
    Published: 1985
    Released on J-STAGE: March 19, 2009
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  • 1985 Volume 50 Issue 3 Pages e1c
    Published: 1985
    Released on J-STAGE: March 19, 2009
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