1) The amount of total hydroxyproline and nitrogen in post-mortem tissue cultures of human fibroblasts from 14 subjects (foetuses, prematures, sucklings) affected mainly by various forms of anoxic syndromes and inborn developmental defects was studied.
2) Tissue culture fibroblasts from spleen, thymus, pericardium, peritoneum, pleura, diaphragm, skeletal muscle and myocardium were studied.
3) By comparing the values Hypro/N, Hypro/10
7 cells it was proved that there is no difference between fibroblasts of the same individual, between fibroblasts from the same organs of various individuals and between fibroblasts of various organs of various individuals.
4) A difference between tissue cultures from living donors and from pos-tmortem tissue cultures was not proved either. The average value of the amount of μg Hypro/mg N in post-mortem cultures was 3.1±0.3 (x±tsx), in the ratio μg Hypro/10
7 cells 16.2μg (geometrical mean) with a lower confidence limit of 13.5 and the upper one of 19.4.
5) The time of tissue sampling after death had no influence on the amount of hydroxyproline in fibroblasts up to 3 days after death.
6) These conclusions are valid also for the index N/10
7 cells with the exception that the difference related to cultures from living donors was not proved in the spleen only.
7) These results justify a wider use of tissue cultures from necropsy material for experimental and physiological study, particularly in organs which can be dealt with only with difficulty and for the study of pathogenesis of diseases with hereditarily conditioned disturbances of connective tissue.
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