1. Several staining and histochemical properties of the components of Golgi complex of the fibroblasts cultured
in vitro and the ascites tumor cells of the mouse were studied.
2. When examined with phase optics, the Golgi complex of the living fibroblast consists of canalicules and filaments, while that of the tumor cell is composed only of filaments. The filaments are finer than the mitochondria and blurred in outline. Since the walls of canalicules show the same staining and histochemical properties with the filaments, the canalicules are thought to belong to a category of filaments, which are either dilated locally or are dilated whole-length.
3. The Golgi filaments (the word is used in the broad sense, as indicated above, in this and the following sections) are blackened more readily than mitochondria by the addition of osmium tetroxide. They are stained red with Altmann's acid fuchsin as mitochondria.
4. Janus green stains the Golgi filaments as it does mitochondria. In the fibroblast, a higher concentration of the dye is evidently required to stain the Golgi filaments than mitochondria.
5. The Golgi filaments visualized by blackening with osmium, by staining with acid fuchsin and by vital staining with Janus green are distinguished from mitochondria, as the former are more slender and less smooth in outline than the latter and are possessed of a characteristic of gathering in the vicinity of the nucleus.
6. The Golgi filaments are stained vitally with neutral red, and vitally and metachromatically with methylene blue, trimethylthionin and toluidine blue.
7. The ground substance of the Golgi area is often darker or lighter under the phase microscope than the remainder of the cytoplasm. It becomes darkened with osmium and is stained
post mortem with acid fuchsin and
intra vitam with trimethylthionin.
8. The Golgi filaments in the both types of cells are not stained with iron hematoxylin and resorcin-fuchsin. They do not react positively to the acid hematein test, nor to the periodic acid-Schiff and alkaline phosphatase techniques. They are weakly stained with sudan black and Nile blue in the calcium-formol fixed materials.
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