This basic study was conducted in order to introduce the subcutaneous inoculation method devised by the authors and Chang's intraperitoneal method. Various therapeutic agents were tested by the two methods. The screening tests by intravenous, corneal, subdural, intraperitoneal and other inoculation methods used by other investigators are discussed. The value of the method of subcutaneous inoculation of dilute emulsion of the authors and the method of intraperitoneal inoculation of concentrated emulsion of Chang is discussed from the standpoint of time relation, technique, trustworthiness, quantitative relationship and reproducibility.
The observations are summarized here.
1) The time required for evaluation is the same with either method but by using a 10-3 emulsion in the subcutaneous inoculation method, it is possible to shorten the time.
2) With the subcutaneous inoculation method, evaluation is limited to a single point, the site of inoculation, and as measurement can be made accurately with a gauge, the technique is simple and accurate.
3) The subcutaneous inoculation method revealed the limits of the onset inhibiting action of known agents as INAH, SM and DDS with both the dilute and concentrated emulsions. It is believed that this finding is sufficient evidence for trussing the accuracy of the evaluations.
4) For screening very small quantities of synthetic or extracted samples, subcutaneous inoculation of 0.2 ml of 10-4 emulsion is more appropriate than the intraperitoneal inoculation method.
5) Reproducibility is more accurate with the subcutaneous inoculation method which is technically simpler and evaluation is more objective and accurate compared with the intraperitoneal inoculation method.

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Cultivation of murine leprosy bacilli in vitro has not proven entirely satisfactory. Studies were conducted on in vivo cultivation as an indirect aid in clarifying the mode of propagation of the bacilli. In order to avoid the partial observations resulting from in vivo methods used heretofore, phase contrast microscopy and the slide culture method in vivo were utilized. The following results were obtained.
1) Murine leprosy bacilli show elongation, branching and granules formation similar to growth of other acid-fast bacilli on the slide inserted under the skin of the white rat.
2) It is suggested that murine leprosy bacilli will multiply even in the absence of body cells if there is adequate infusion of body fluid in a susceptible animal (white rat) under conditions where invasion of body cells is obstructed.
3) Propagation of murine leprosy bacilli in murine leprosy sensitized white rats is slow and minute compared to the non-sensitized control.

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We synthesibed N'-ethyl-p-aminobenzensulfonamide and N', N'-diethylbenzensulfonamide
for the treatment of Leprosy.
N'-ethylbenzensulfonamide and N', N'-diethylbenzensulfonamide prevent tolerably the development of Murine Leprosy.
The effect of them upon murine leprosy is almost the same degree.
On the therapeutic test of leprosy murine Pashydrazide is pretty well.

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DDS prevent tolerably the development of Murine Leprosy.
The effect of DDS upon murine leprosy is almost the same as that of promin, in case of Usmy same dose of free DDS and DDS which is contained in Promin.

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It is neadless to state that the susceptibility of the experimental animal greatly influences the experimental results. In the study of marine leprosy, the accuracy of the results will no doubt be greatly enhanced if white rats or mice of a strain which are highly susceptible are used. From this standpoint a comparative study of the susceptibility to murine leprosy was conducted in 4 strains of mice, C 57 EL/6, C3H, dd and the Wister strain of white rat. No significant difference in the degree of susceptibility was observed between the different strains.

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The influence of age and sex of the experimental animal on susceptibility to murine leprosy was studied in the dd strain (uniform strain) mouse. The animals were divided into 4 groups, that is, immature, mature, male and female. No significant difference in susceptibility was noted between the groups.

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Subsequent to the introduction of the antigen for complement fixation test in 1951, Ogata reported his agglutination method.
The authors made a comparative study between Ogata's method and Honda's (complement fixation) method.
Of 62 lepromatous cases, the complement fixation was positive in 55 and agglutination was positive in 44 cases ; of 29 neural cases, the complement fixation was positive in 16 and agglutination was positive in 9 cases; of 5 macular cases, the complement fixation was positive in 3 while agglutination was not observed.
Both methods. were negative in all non-leprous cases examined.
From these results the authors believe that Honda's complement fixation method is more favorable than the method of Ogata.
The influence of the addition of lecithin to the cephalin antigen was studied.
Five in. patients at the Kyoto University Hospital were selected. Blood was drawn from these patients and mixed sera were prepared.

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