The electromyography of 6 macular, 1 neural and 5 tuberous cases was measured upon the lesions of the forearms and lower legs under the forceless and gradually contracted conditions. The wave and the distance of the discharge were compared with those of the normal people.
1. The wave measured upon 6 places of both forearms of serious tuberous patients compicated with mutilation of the fingers and the claw-hands showed the wave of Grouping-voltage, though some differences were observed.
At the time of the quietness, no spike discharge was observed.
2. No difference was observed between the results of slight disformed patients and those of the normal people.
3. The waves and the distances of discharge examined by electromyography presented no difference, unless the the patients were complicated with extreme disformities.

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A relatively small quantities of TB₁ and INAH were administered. TB₁ produced an excellent effect. In this paper, the treatment effect and experimental results of resorption, discharge and organ concentrations of these two chemical agents are reported.
1. TB₁ administered 50-100mg daily produced an excellent effect.
2. Treatment with INAH was effective upon tuberculoid leprosy. Other types were unchanged or aggravated by this treatment.
3. Toxic manifestations of these drugs were insignificant.
4. The blood concentration of TB₁ was recognized after 30 minutes both in the normal and in leprosy patients. It reached the highest after 5 hours in the normal and after 24 hours it was still detected in both cases.
5. The discharged dosis in urine after 24 hours was 27.7% in the normal, and 5.37% in leprosy patients.
6. TB₁ was found in a large quantity in the liver, kidney, lung and heart, but not at all in the skin or muscles. The organ concentration was increasing as the administration was continued.
7. The tuberous and macular lesions treated with TB₁ did not contain this drug.
8. After the various admintrations of INAH to adult rabbits the variation of blood and organ concentrations was measured.
The results are presented below.
a) After 1 hour the blood concentration presented 13 I/u by subcutaneous injection, 8 I/u by per os admimstration. In every administration mode it decreased to 2 I/cc after 16 hours. The decrease was relatively gradual in subcutaneous injection, prompt after 1 hour in intravenous injection and very slowly in per os administration.
b) After 16 hours subcutaneous injection maintained higher organ concentration than other two methods.
c) By per os administration the concentration war high in the kidney, skin and muscles. As for the periodical variation it increased in the liver and adrenal, and remarkably decreased in the kidney, muscles, brain and lung.

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From Oct. 1953 to Feb. 1954 224 eyes (63 eyes of neural and macular and 163 eyes of tuberous patients) of 114 leprosy patients and 146 eyes of 72 non-leprosy people were examined on the bacterial flora in the conjunctival sacks. The materials were cultivated upon the plate blood-agar. For the investigation of the leprosy bacillus, the smear was stained with ZiehlNeelsen method. The results are summarized below.
1) The leprosy bacillus was negative in all cases.
2) By the cultivation, Cor., Sta, alb. et aur., Pn., Str, haem., vir. et nonhaem., K. W., M. A., Friedländ., Micrococ., Pyocya., Gram (+) and (-) Bac., Neisseria and Blastomyces were detected.
3) The kinds of organism and the frequency of positive result were more remarkable in leprosy patients than in the normal people. There existed no marked difference between M N and L types (Fig. 1). The average number of the kinds of organisms in one eye is 0.95 in non-leprosy people, 1.23 in the L type and 1.71 in the M N types (Fig. 2).
4) In the patients in whom conjunctivitis was not recognized, not any or few numbersof organisms taken for the pathogenes or faculutative pathogenes were certificated, but Staphylococcus albus and other non-pathogenies were found out, without any difference in the frequency between leprosy or non-leprosy people. (Fig. 3). The average number of bacillus kinds in one eye is 0.83 in non-leprosy people, while, 1.04 in L and 15.0 in M. N. patients. The average number of the kinds of organisms in one eye was 1.04 in L type and 1.50 in M. N. types, to 0.83 in the normal people (Fig. 4).
5) 14 kinds of organisms were detected from the leprosy patients with chronic catarrhal conjuctivitis. The organisms detected in the highest frequency were Cor. (69.9%), Sta. alb. (25.3%), Sta. aur. (19.2%), Pn. (18.5%) and others (Fig. 5). The average number of bacillus kinds in one eye with conjunctivitis was 1.69, while it is 1.23 in leprosy patients without conjunctivitis.
6) From the patients with lagopThthalmos and ectropion 15 kinds of organisms were detected. The turn of the frequency and bacillus kinds were similar to that in those who had chronic catarrhal conjunctivitis. (Fig. 7). The average number of bacillus kinds in one eye with lagophthalmos and ectropion was not so different from the above case (Fig. 8).
7) Phthisis bulbi and anophthalmos always complicated with chronic catarrhal conjunctivitis. In these cases the detection percentages of organisms were as following; Cor. (75.0%), Sta. aur. (40.0%) Pn (20.0%) etc. (Fig. 9).
8) The growth of any bacillus could not be observed by culture from the material of one case of conjunctivitis with tuberculoid leprosy.
9) The above results teach us that the bacterial flora of the conjunctival sacs have a relation with lagophthalmos or ectropion and the latter diseases were connected with chronic catarrhal conjunctivitis, which is provoked not only by simple outer stimulations, but by the bacteria in the conjunctival sacs.

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It is most desirable for the screening test of chemical agents to abbreviate the experiment process and to provide the simple standards to judge the results. The following experiments were carried out to find out the murine bacillus strain, inoculation site and dosis which can satisfy those conditions. All the experiments were done with mice.
1) Bacillus Strain: By subcutaneous inoculation, the virulences of Kumamoto, Hawaiian, Keishicho and Fukuoka strains were compared.
The results presented that Fukuoka strain is weaker, and the other three strains have the similar pathogenity. (Tab. 1, 2, 3, 4)
2) Inoculation Site: Subcutaneous inoculation produced leproma at the site in an early period which can be observed exactly. (Tab. 1, 2)
Intraperitoneal and intravenous injections give a rise to the disease in various organs after long while. The predilected organ can not be found and the results are shown by bacillus distribution index and not by a practical standard. (Tab. 5, 6, 7, 8)
3) Inoculation Dosis : 0.2 ml of 10²-10⁴ diluted leproma suspension gives a rise to the disease 2 or 3 months after subctaneous inoculation. When the suspension decreases to 10⁶-10⁸, the longer period is necessary to produce the disease.
By intraperitoneal injection, 0.5 ml of ×50 diluted suspension produced the disease after 3-4 months. 3 months after intravenous injection 0.2ml of 10² suspension gives a rise to visible changes in the 30% of the viscera. It will take longer period to recognize lesions in all cases.
The above results ead us to conclude that the most adequate experiment is to be carried out with Hawaiian strain on account of its international value by subcutaneous inoculation in the dosis of 0.2ml of 10²-10⁴ diluted suspension, and to observe the development for 3 months.

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111 patients (tuberous 109 and neural 2) admitted in National Leprosarium Oshima Seishoen were received eyebrowplastic operation.
1) 110 patients received autohairtransplantation presented favorable results in all cases. In 1 case the hairs were transplanted upon the hyposthetic part, in which sensibility was recovered after a year.
2) Homohairtransplantation carried out upon 5 patients failed in all cases.
3) On pedinculated autoplantation, one case was succeeded and the other one underwent necrosis and scarformation. The latter case received autohairtransplantation with success. In short, hairtransplantation produced favorable results upon the lesion of alopecia leprosa, as well as upon the normal.
Eyebrowplastic operation on leprosy patients is the most adequate treatment not only in the function but also in the cosmetic view, so as to ameliorate the mental agonies.

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From the idea that an antileprotic drug may be found out from the medicines for tuberculosis, the treatment of leprosy with a new chemical, acidomycin, was carried out in three leprosaria and a laboratory. In this treatment it is specific to inject this medicine intracutaneously in the lesions or in the normal skin. 66 patients (lepromatous 39, tuberculoid 20 and neural 7) were administered with 20-193 injections of 200-3865mg acidomycin. The results were remarkably improved 16, moderately improved 13, slightly improved 10, unchanged 20, aggravated 4 and unclear 3. Acidomycin was more efficacious upon tuberous than upon neural or tuberculoid lesions and fresh lesions responded well to it.

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