A new ground medium was consisted of Kirchner's medium added with serum and extract of murine leproma. Various amino acids, vitamins, phthiocol, 6-amino capron acid (ACA) were mixed to the above ground medium. Besides the oridinary method, double layers method (Fig 5), double tubes method (Fig 6) and intraperitoneal culture in white rats were applied. The raw or X-ray treated murine bacilli were cultured without any multiplication.
The human bacillus was inoculated upon the double fluid medium added with sphingomyelin and the result was negative.
These results of the various cultures lead us to have disapproval against the possibility of in vitro culture of the human and murine bacilli supported by some workers.

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1) The results of eye-reaction (subconjunctival injection) carried out with Mitsuda's reaction agree with those of the latter, namely, negative by lepromatous and positive by tuberculoid and neural leprosy.
2) In the case of lepromatous leprosy of which lepromatous infiltration has been absorbed and Mitsuda's reaction has become positive, the eye reaction also presents positive.
3) The leprous pannus in the cornea does not always disappear when the eye and Mitsuda's reactions has become positive after the antileprotic treatment, because of the vacuolization of bacilli and the presence of vacuolized lepra cells.
4) One case is reported in which the eye reaction was useful as a mean of diferenciating the leprous or nonleprous nature of the swelling in the eye with a lepromatous patient whose diagnosis was under doubt of neural type and Mitsuda's reaction positive.

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Pharmacologic experiments on D. D. S. carried out in our laboratory presented the following results :
1) D. D. S, inhibits the growth of staphylococcus in vitro in 0.1-0.5 mg %, but effective only in higher concentration upon the tubercle or the smegma bacillus.
2) Below the concentration of 1mg %, it shows no influence upon the development of chick embryo heart tissue cultured in the test tube.
3) The per os administered D. D. S. concentration in the various organs of rabbits reach the highest after 3 hours. The concentration in the liver and kidney is as much as two or three times as that in the blood. In the skin and nerve it reaches to 0.2mg% and produces the therapeutic significance.
4) The blood concentration of D. D. S, in patients administered per os three times a day shows no marked up and down throughout 24 hours.
5) The 70-90% of per os administered dose of D. D. S. is excreted in urine, of which 90% is excreted as D. D. S..
6) Between D. D. S. and blood proteins, absorption takes place in acid medium and both absorption and chemical combination occur in alkaline medium.

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1) This paper summarizes the history of D. D. S. antileprotic treatmentt and introduces a part of studies on D. D. S, reported by the workers in many countries.
2) 38 leprous patients containing 22 lepromatous, 6 tuberculoid and 1 neural were administered with D. D. S. over the period from 1 month to 18 months.
3) The per os administration of D. D. S. began with 15mg per day and increased to 30mg, 50mg and 100mg at the interval of 2 months, and 100mg was continued. 2 cc of D. D. S. saline emulsion was applied to intramuscular injection.
4) In the 22 lepromatous treated per os with D. D. S., 4 improved strikingly, 8 improved, 7 a little improved, 3 unchanged and non aggravated.
5) The treatment result of D. D. S, injection will be reported later.
6) Toxic manifestations were observed such as slight anemia in 3, neuralgia in 2 and palsy of sural nerve in 1 patients. Erythema nodosum leprosum appeared in 4 cases.
7) D. D. S, has an excellent therapeutic effect upon leprosy and can be administered without any fear.

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1) In 65 patients (74 eyes) of leprous eye disturbances, the local treatment of cortisone produces the most remarkable effect upon acute iridocyclitis, upper scleritis due to erythema nodosum leprosum and scleritis.
2) It is ineffective upon leprous keratitis.
3) ACTH was applied and presented favorable results on 3 patients (5 eyes) of iridocyclitis and scleritis caused by erythema nodosum leprosum in which cortisone had produced no effect.
4) The excellent efficacy of ACTH and cortisone upon erythema nodosum leprosum and accompanying acute iridocyclitis supports Mitsuda's assumption that the etiology of erythema nodosum leprosum should be considered an allergic inflammation.

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This paper reports the results of experiment if the murine bacillus can get resistance against S. M, and if the combined administration of INAH and S. M. can prevent the murine bacillus to obtain the resistance against INAH.
A. On the resistance of the murine bacillus against S. M. White rats inoculated with the murine bacillus subcutaneously were injected S. M. 5mg per day, six days in a week.
The leproma which had developed after the total 610mg S. M. and that had developed after the total 650mg S. M. and then received no treatment for 150 days were selected as the bacillus suspensions of respective dilutions for the next generation. These two strains were inoculated to white rats and S. M. of the same dose was injected in the same manner for 150 days.
The results show that the onset of the disease was inhibited to the same degree in both strains as S. M, sensitive strains reported by us (La Lepro, Vol. 23, No. 2, p. 23). The murine leprosy bacillus can not be strongly inhibited by S. M, in its multiplication, but can not get any resistance. (Fig. 1-3)
B. On the Prevention of INAI I Resistance by the Combined Administration of INAH and S. M.
The total 50mg INAH and 200mg S. M, were administered (INAH 4mg/ k and S. M. 20mg/k, daily, 6 days in a week) for 60 days to white rats with large leproma. Then the remaining leproma was extirpated and inoculated to white rats.
The lepromas in the 2nd generation were extirpated again and diluted and inoculated to white rats. The animals were administered with INAH from the next day for 150 days.
The result shows that an appreciable resistance could be observed compared with the sensitive strain reported by us (La Lepro, Vol. 23, No. 1, p. 22), but the onset was more clearly prevented than the resistant strain (La Lepro, Vol. 23, No. 1, p. 22). Therefore, INAH resistance can be prevented more strongly by the combination of INAH and S. M, than by the single usage of INAH. (Fig. 4 )

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