I observed an autopsy on Mitsuda's reaction negative case clinically diagnosed as neural leprosy. There were found lepromatous changes which show lepra cells with vacuolated appearance in ulnar nerve and femoral lymph node.
This fact shows the specifity of Mitsuda reaction for confirming the type.

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The cultivation of human and murine leprosy bacilli was carried out in test. tubes, capillary tubes and slide cell culture method with media of Kirchner, Lockemann, Long and Seibert and T. G. C. added with small quantity of serum, serumalbumin, CaCl₂, polytamin, maliamin, milk, extract of squash and other substances, but no multiplication of bacilli was observed.
These results can not help confirm the report of Hanks that the multiplication of the leprosy bacillus in artificial media has never been certified up to now even by the microscopic examination.

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The results of Smith and Scott's methcd of Middlebrook and Dubos' blood cell agglutination with 121 leprous patients were as following:
1) The positive results range from 8 to 2048 times diluted sera, of 86.5% of tuberous and 53.2% nervous or maculous patients.
2) As the sensitizing antigen, the carbonhydrate fraction of the tubercle bacillus components is superior to the protein fraction.
3) The water extract of leproma, the carbonhydrate or protein fraction of it, the urine of lepra or tuberculous patients are not available for the sensitizing antigen.

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The suspension of fresh human leproma inoculated subcutaneously or intraperitoneally into 14 golden hamsters could not produce the positive results. The hamsters inoculated with the murine bacillus suspension gave rise to leproma under the skin, and the successive inoculation was successful. In the hamsters intraperitoneally inoculated striking multiplication of bacilli was recognized in the liver and spleen. But there develops a noticeable tendency that a small quantity of inoculated bacilli could not cause infection at the later generation.

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The experiment was carried out to investigate wether the ocular tissue which has strong sensitivity to many pathogenic organisms may offer a favorable media for the multiplication of the human and murine lepresy bacillus, indifferently to the race specificity of animals, to give rise to the leprous change, and to examine the influence of the trauma or the general infection upon the leprous change of the ocular tissue. The results obtained were as following :
1) The ocular tissue of rabbits, guinea pigs and white rats showed no specific affinity to the human and murine leprosy bacillus, exceeding the limits of race specificity.
2) The trauma given to the eye aggravated the disease, but no favorable effect was seen on the multiplication of bacilli.
3) The general infection of white rats inoculated with murine bacilli into the anterior chamber strengthened the leprous change and the multiplication of bacilli.

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The treatment of erythema nodosum leprosum with N M C presented a good result. We can apply this drug, continuing the treatment of leprosy, to both acute and chronic cases, without any side reaction. The agency of N M C should be comprehended to neutralize the toxic substance produced by allergic reaction.

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