(1) Fourteen specimens of the sites of the Mitsuda reaction of the seven lepromatous cases which had become posive with chaulmoogra oil treatment were removed from 3 hours to 82 days after the injection and were studied histopathologically.
(2) The nonspecific acute exsudative inflammation with marked infiltration of neutrophile leucocytes and histiocytes was observed until 48 hours after injection, but it disappeared gradually thereafter.
(3) The tuberculoid granulome appears 8-14 days after injection, and this tuberculoid change is regarded as the histological manifestation of positive Mitsuda reaction.
(4) The caseation was not found in the tuberculoid granulome with some exception.
(5) The injected bacilli in the tissue were found even after 82 days.
(6) It is considered as the evidence of getting resistance to lepra bacilli that the subsided lepromatous case, which formaly was negative, give positive Mitsuda reaction.

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Sixteen biopsy specimens of the sites of the Mitsuda reaction were removed from six lepromatous cases and were studied histopathologically.
Simultaneously boiled Mycobacterium lep. murium and B. C. G. (either in one case) were injected and studied as control.
The results are as follows.
(1) An acute exsudative inflammation with neutrophile leucocytes and histiocytes were observed after 48 hours. But the grade of inflammation was slighter than that of the Mitsuda positive case.
(2) This inflammatory process disappeared quickly thereafter and left only slight cellinfiltration around small vessels, sweat glands and hair follicles.
(3) No tuberculoid granuloma was found. On the contrary the tuberculoid granuloma could be seen with B. C. G. and Mycobacterium lep. murium. This fact shows the specificity of Mitsuda reaction.
(4) The injected lepra bacilli disappears sooner than that of neural and tuberculoid case.
(5) The negative reaction in the lepromatous case is the evidence of lack of resistance against lepra bacilli.

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The present paper is a report on the preventive and therapeutic actions of the newer chemotherapeutics, Promacetin and Proethyl, and, especially, on their combined effects with Cepharanthin on the development of the experimental rat-leprosy.
The results were as follows :
(1) Promacetin and Proethyl apparently possessed the preventive and therapeutic properties against the rat-leprosy, and the improvement noted in the animals treated with Promacetin was certainly more striking and rapid than that observed in those treated with Proethyl.
(2) So far as the present combined dosage of Cepharanthin, it did not potentiate the action of Promacetin or Proethyl, and appeared rather to be antagonistic against that.
(3) Although the therapeutic effect of Cepharanthin on the rat-leprosy is much or less able to be expected, the further trials are required as to its dosage.

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In vitro expertment on the absorption of sulfones, especially of promin and promizole, to red blood cells supplied us following results:
(1) Both promin and promizole are absorbed to red blood cells, and the ratio is larger with promizole than with promin. And the absorption occurs according to Freundlich's absorption isoterm.
(2) The absorption of these sulfones is inhibited by the presence of blood plasma.
(3) Both drugs are combined well with serum albumin, but not at all with serum euglobulin. The mesurement with Tiselis' electrophoresis showed that promizole is stronger in the combination than promin.

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The treatment and inhibitory effects of INAH upon rat leprosy are as following:
1) The administration of lmg. per day, six days in a week, was continued for five months. As the results, leprous ulcer was healed, leproma resorbed. The lepromas enveloped with thick connective tissue can not be easily resorbed, but they became harder and smaller in size.
2) Histological examination revealed storong proliferation of connective tissue in the lesions of local skin and regional lymph nodes. There was no appreciable lesion in the internal organs.
3) The longer the administration was continued, the more markedly disformed, granular marine leprosy bacilli were observed. But still they kept surviving force after each administration of 50 mg, 90 mg and 134 mg. There resuts were acertained from the seccessive inoculation with the treated leproma.
4) In the ustreated control animals the cases of healing ulcers were observed, but in no case the leproma was completely resorbed, and bacilli and lesions were found in the viscera.
5) The animals which received treatment from the next day of inoculation produced no onset of the disease. But those which were inoculated with 20 thick bacilli susupension showed a slight difference from the untreated control animals.
To summarize the results, INAH is quite effective upon the murine leprosy. Though promin and tibione are favorable upon human leprosy and not upon marine leprosy, INAH produces better effect upon murine leprosy than human leprosy. From these facts, chemical agents useful to human leprosy are different from those favorable to murine leprosy. Therefore, animal experiments of leprosy treatment can not be applied to the research for a new drug, but to the explanftion of the bacteriostatic mechanism of the drugs against the marine leprosy bacilli.

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During March, 1952-September 1952, X-rayine to the leproma of humanbody, comperatively fresh, was carried out, and I observed its influences.
Consequently, there observed some changes connected with dimention of leproma, histological states etc., to be reported.
Namely, the slight reduction of leproma, increasing of the giant-cell, in the lepromtissue, increasing of thec onnective tissue, decreasing of the round cell, atrophy of nuclear observed, but change of form of bacillus leprae in the lepra-cells, furthermore, skin-injury accompanied by the X-raying was so strong that an effective influence can not be expected.

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36 leprous patients with neuralgia and arthrodynia were treated with orally administered Salicylamide.
The results are follows:
1) 20 of 36 patients are improved by this treatment.
2) The administration of 3gm, daily is not enough. The required dosis is 6 to 8gm. daily.
3) In 16 patients (44.4%) there are side-effects of this drug, mostly consisting of symptoms of stomach.

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We have undertaken the following experiment in order to investigate the effect of the Hydrazide for leprosy from May, 1952.
We selected the 20 lepromatous leprosy who had not ever received any treatment for leprosy.
The way of administration were as following : at first we gave them 1mg/kg per os and then after one month gave them 5 or 6mg/kg.
The results were summarized as following :
1. The resorption of nodules and infiltrations were observed in 13 patients (65%)after
4 or 6 months, but when they more over continued this treatment we found relaps of leproma in 12 patients after 2 months.
2. We also observed this finding in the histological and bacteriological examination of skin, nasal mucous membrane and blood.
3. This new leproms were resorbed by promin treatment within 2 or 3 months.
4. Erythema nodosum leprosum was observed in only 2 cases (10%).
5. Mitsudas reaction showed no change.
6. Liver and kidney function test showed no change.
7. On second function we could not experiment any important complcation.
8. Lepra bacilli of skin and nasal mucous membrane after 5 months decreased a little but later had a tendency to increase.
9. On blood findings we observed that erythrocytes and hemoglobin decrease a little and lymphocyte and eosinophile cell increase but neutrophile leucoytes decrease.
10. Result in total
I. Improvement 13 cases (68%)
a. Improvent 1 case (8%)
b. Relaps 12 cases (92%)
II. Worse 6 cases (30%)
III. Stationary 1 case (5%)
In short this observation is carried out 10 months in our experiment which leads to the following concusion :
Hydrazide is effective for lepromatous leprosy after 4 or 6 months but relaps occur before long (about after 2 months, ).

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