The influence upon the immunity caused by the repeated intracutaneous injection of Dharmendra's antigen had been studied experimentally in guinea pigs, and the results obtained were as follows;
1) Fernandez's reaction provoked by Dharmendra's antigen was increased in BCG-vaccinated animals, when the intracutaneous injection of Dharmendra's antigen was tried repeatedly. On the other hand, the influence upon tuberculin reaction was not found either in vaccinated animals or in unvaccinated animals.
2) When the dead BCG-suspension was injected intracutaneously and these injections were repeated instead of Dharmendra's antigen, any influence upon tuberculin reaction and Fernandez's reaction was not found in BCG-vaccinated animals, but both reactions in unvaccinated animals were increased and converted to positive.
3) All experimental animals were challenged by tubercle bacilli for the comparing of the antiinfectious ability against tuberculosis. These abilities were mostly related equivalently to the strength of tuberculin reactions, and were not related to the strength of Fernandez's reaction provoked by Dharmendra's antigen.

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The influence of the repeated injection of Dharmendra's antigen upon Fernandez's reaction in BCG-vaccinated guinea pigs had been reinvestigated experimentally, and the obtained results were as follows;
In BCG-vaccinated guinea pigs, it was found that Fernandez's reaction caused by the repeated intracutaneous injection of Dharmendra's antigen was stronger than fhat first reaction before the repeated injection of antigen.
Nevertheless, the anti-infectious ability against tuberculosis in the increased reaction group was equivalent as that in control group, so that the influence of the repeated injection of Dharmendra's antigen upon the anti-tuberculosis ability was not recognized.

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Sera obtained from 86 leprosy patients were tested for the presence of circulating antibody by means of the Ouchterlony method. The antigens employed were filtrate Preparations of Youman's culture media of the H37Rv strain, M. bovis (BCG, 263), two strains of atypical acid-fast microorganisms isolated from the sputum of tuberculous patients and saprophytic microbacterial strains (scotocromogenic acid-fast bacilli and milk strain). The results were as follows:
Fifty-four Specimens of serum from 86 leprosy patients reacted with the above antigens on agardouble diffusion test. Thirteen specimens of these 54 cases gave a positive reaction only with the antigen of H37Rv, and five specimens reacted only with the antigen of M. bovis. Two of the remaining 36 specimens reacted only with the antigens present in atypical acid-fast bacilli. The last 34 specimens of sera reacted with the common antigens present in H37Rv, bovine strains (BCG, 236), atypical acid-fast bacilli, milk strain and Dharmendra antigen.
Antibodies to the antigens of atypical mycobacteria were detected also in sera of tuberculous patients as indicated in the preceding paper. The positive rate of the Ouchterlony test in sera of tuberculous patients was less than that in leprosy patients. In so far as these tests show, there is significant differences between the positive cases in the Ouchterlony test obtained in lepromatous and tuberculoid leprosy. For example fifty-one of fifty-four cases of a positive Ouchterlony test were observed in lepromatous leprosy and only the remaining three cases in tuberculoid. Sex, age and process of diseases as will as associated tuberculosis do not effect the results of the Ouchterlony test. Sera taken from healthy nurses did not show any precipitation band with the exception of one case. In this case no evidence of tuberculous disease had been noted in the past.

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Sera were obtained from 74 leprosy patients of the Zensho Leprosarium and were tested for the presence of circulating antibodies by means of the Ouchterlony method. The results of this experiment were compared with the results obtained from the sera of leprosy patients of Fukusei Leprosarium. For control, sera taken for the Wasserman test, including pregnant women's sera, were used. The antigens employed are culture filtrate of H37Rv, BCG, atypical acid-fast organism and milk strain. In addition CF and PPDs derived from human tuberculous strain were employed. The results are as follows :
1) Twenty-seven specimens of serum from 74 leprosy patients reacted with the H37Rv antigen, fourteen specimens with the BCG antigen and twenty-three with the atypical mycobacterial antigens. Positive percent of the Ouchterlony test between leprosy sera and antigens of mycobacteria used in this experiment was almost similar to the results in Fukusei Leprosarium.
2) There was a marked difference between sera of lepromatous and tuberculoid leprosy. The Ouchterlony precipitation reaction between sera of lepromatous leprosy and antigens of mycobacteria was almost positive while in the reaction between sera of tuberculoid cases and mycobacterial antigens many negative cases were shown.
3) Patients sera in macular (TM-type) and lepromatous leprosy (L-type) indicated almost the same results by Ouchterlony precipitation technique. However, the results of the experiment with sera of anesthetic leprosy patients was quite different, and it seemed to be not related to serologlc study.
4) Sera of pregnant women and other patients taken for the Wasserman test did not show any precipitation band.
5) Some specimens of sera of leprosy and control patients reacted with the fresh milk antigen.
6) The sera of the lepromatous leprosy (L-type) reacted with PPDs antigen and were more marked than CF antigen.

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