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Factors, involved in the procedures for making bacillary suspensions, affecting the elongation of M. lepraemurium in vitro, were studied and the following results were obtained.
1. The most significant elongations of the bacilli were observed when the bacillary suspensions were made by a mild procedure in which a leproma was cut with scissors and suspended in 0.1% bovine albumin V (Armour)-saline by pipetting, or was gently ground and homogenized in a mortar for one or five minutes.
2. Slight elongation took place in the cases of experiment in which partially purified bacilli or bacilli obtained from the homogenate of fifteen minutes' ground in a mortar were used.
3. There was a remarkable difference between two kinds of medium used for observing elongation phenomena; in the original Kirchner medium, elongation occurred at pH6 and in the enriched Kirchner medium pH7.
The data mentioned above, should contribute toward the experiment for cultivation and animal experiment of M. lepraemurium.

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Effects of the depth of medium and CO₂ gas on elongation of M. lepraemurium in vitro were studied and the results obtained are as follows;
Elongation of the bacilli was not stimulated under the culture conditions in CO₂ gas as well as air atmosphere. The most significant elongation was observed when the bacilli were cultivated in 9ml of the Enriched Kirchner medium pH7, per tube, covered with a rubber stopper which had been used previously.

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