生物物理化学
検索
OR
閲覧
検索
1 巻 , 1 号
選択された号の論文の12件中1~12を表示しています
    • |<
    • <
    • 1
    • >
    • >|
  • 兒玉 桂三
    1 巻 (1951 - 1953) 1 号 p. 3
    公開日: 2009/03/31
    ジャーナル フリー
  • 島尾 和男
    1 巻 (1951 - 1953) 1 号 p. 4-14,46
    公開日: 2009/03/31
    ジャーナル フリー
  • 小川 新吉, 近 新五郎, 浦田 卓, 木村 武
    1 巻 (1951 - 1953) 1 号 p. 15-22
    公開日: 2009/03/31
    ジャーナル フリー
    1) 正常人血清を1/20m燐酸緩衝液で透析し,透析前後に於ける試料の變化,泳動時間によるPatternの變化及び試料蛋白濃度の増減に伴うPatternの變化に就て實驗し次の如き諸事實を確認した。
    2) 透析後の試料蛋白濃度を2.2~1.5g/dlにするには透析前の蛋白濃度を2.2~1.7g/dlとすればよい。
    3) 透析前の試料の量を12cc前後にすれば透析後の試料の量は何れも増加するが,原血清の増量は最も大きく,3~7倍稀釋で2.5~2.0ccであつた。
    4) 3~4倍稀釋の透析後試料の蛋白濃度を日立蛋白計で測定した値は±0.2g/dlの誤差範圍でキィルダール値と一致した。從つてこの稀釋濃度のものなら日立蛋白計を簡易蛋白測定法として使用し得る。
    5) γ-gl.の分離は最も遲いが泳動後60分までには完全に分離する。
    6) γ-gl.の相對的易動度は泳動に伴い減少するが90分以後は安定する。その他の分屑の相對的易動度は泳動時間の相違によつて大きな變化を示さない。
    7) 峰の高さ及び底邊の長さの變化と泳動時間との間には一定の關係を認め難い。
    8) 各分屑の面積及び面積比はα-gl.が分離してから後は泳動時間によつて有意義な差違を示さない。α-gl.は主にAlb.より,時に一部β-gl.より分離されるように思われる。γ-gl.,特に上昇側のものは最初の90分まで減少する傾向が認められる。
    9) 試料蛋白濃度の大小によつてPatternの基本型は變化しない。
    10) 試料蛋白濃度の大小によつて各分屑の相對的易動度にも大きな變化は認められない。
    11) β-disturbanceは必ずしもFibrinの析出,對流に關係があるとは決定出來ない事實を確認した。
    12) 各分屑の左右非對稱性は泳動時の恒温槽温度及び使用緩衝液のイオン強度と密接な關係のあることを知つた。
    13) 3倍稀釋を中心として,それより稀釋倍數が多い場合も少ない場合もAlb.の面積比は3倍稀釋の場合より高くγ-gl.はより低く出る。
    14) β-anomalyの面積は原血清を除いて,大體試料蛋白濃度の大小と比例して増減する。
    抄録全体を表示
  • 宅間 清, 宮村 正文, 森下 正生, 土屋 順, 日比 弘, 村田 吉郎
    1 巻 (1951 - 1953) 1 号 p. 23-26
    公開日: 2009/03/31
    ジャーナル フリー
    We observed 11 patients who stayed in the Yamada Red Cross Hospital from June 1949 to December 1949, and measured their plasma protein fractions by electrophoreses. At the sametime, we examined dextrose double examination (Hagedorn-Yensen), serum N-P-N, serum-Cl (Koranyi-Ruszniak), serum-Ca (Krammer-Tisdall), sedimentation rate of red blood cells (Westergren), plasma bilirubin (Meulengracht), serum-Co (Inoue) and serum-Cd (Fujita-Iwano).
    Conclusion
    1) In biliary liver cirrhosis total plasma protein and albumin decreased (43.5-43 per cent), and especially after every ascites puncture. Alpha-globulin decreased. The increase of beta-globulin was not always proportional to the quantity of plasma bilirubin. Gamma-globulin increased (22.7 per cent). Both died after leaving the hospital.
    2) In biliary liver cirrhosis and subacute hepatatrophia albumin decreased and the increase of beta-globulin was not alway proportional to the quantity of plasma bilirubin. In subacute hepatatrophia gamma-globulin increases (35.5 per cent).
    3) Obstructive jaundice (2 cases)
    The one died from insufficiency of liver function, the other completely cured. Albumin decreased (34.5-42.4 per cent).
    4) Simple jaundice (3 cases)
    All patients were completely cured. In all cases. serum protein did not decreased, but on the contrary, it increased in one of them. In all cases, albumin decreased at first, but after leaving the hospital, a normal conditions was regained. The increase of beta-globulin was almost proportional to the quantity of poasma bilirubin.
    5) Dextrose double examination.
    Fatal cases (3 cases; Staub (-), 2 cases; Staub (+)). Others; Staub (+).
    6) Serum N-P-N.
    In subacute hepatatrophia, the value increased (93.2mg/100cc), in all others, normal.
    7) Serum Cl, normal.
    8) Serum Ca, normal.
    抄録全体を表示
  • 吉澤 久雄, 本田 正節, 堀江 喜一
    1 巻 (1951 - 1953) 1 号 p. 27-29
    公開日: 2009/03/31
    ジャーナル フリー
    1) With a view to making clear the difference between immunisations with living and dead bacteria, we studied experimental typhoid fever of rabbits, especially on agglutination, onset-inhibiting power and alteration of serum protein in electrophoretic analysis.
    2) Dividing rabbits into two groups we vaccinated the first group with weak living S. cholerae suis (R type P 32 strain) and the second group with dead S-type bacteria.
    After vaccinating them, we inoculated both groups with the strong “Hokkaido-Strain”.
    The second group died though they showed strong agglutination. Those in the first group all remained alive, though agglutination was negative.
    3) Total serum, protein increased in all cases except one. γ-Globulin showed the most remakable increase, namely two or three fold. We were not able to recognize quantitative difference in γ-globulin between these two groups.
    However, the shape of the peak showed a difference. In the first group the shape of the peak of γ-globulin is larger on the side of larger mobility, than on the side of a smaller mobility.
    The conditions in those of the second group are completely reverse.
    4) It was supposed that there were some relations among the shape of the γ-globulin-peak, agglutination and general immunisation.
    抄録全体を表示
  • 向井 壽徳, 山田 榮士郎, 工藤 卓哉, 吉田 司, 白井 正敏
    1 巻 (1951 - 1953) 1 号 p. 30-34,46
    公開日: 2009/03/31
    ジャーナル フリー
    We studied the plasma protein fraction of healthy men and of patients of pulmonary tuberculosis by means of Tiselius' apparatus. We admihistered tibione for 1-4 months to the patients of pulmonary tuberculosis, and observed the change of plasma protein fraction, with various clinical investigations.
    The results are as follows;
    The total protein, albumin and albumin-globulin quotient decreased, but γ-globulin and fibrinogen increased more in the plasma protein fraction of the cases of pulmonary tuberculosis than in those of healthy men.
    When tibione gave effective results, pathological change of plasma protein fractions had a tendency to return to healthy conditions.
    抄録全体を表示
  • 吉田 長之, 住友 健治, 谷 勇
    1 巻 (1951 - 1953) 1 号 p. 35-38
    公開日: 2009/03/31
    ジャーナル フリー
    Davis, et al., describing the Wassermann antibody, said that it has a rather broad range of mobility with its center between beta- and gamma-globulin, although, no separate peak is there.
    The present report also discussed the mutual relationship between serum protein fractions and antibodies in Wassermann, Meinicke and Murata tests.
    Experimental
    Electrophoretic analysis and fractionation were carried out with the Tiselius' apparatus using Schlieren-diagonal method.
    Pooled syphilitic human sera were dialyzed, before electrophoresis, against 1/20M phosphate buffer of pH 8.0.
    Electrophoresis was carried out until the components. were distributed throughout the length of the cell, and then compensated so that the ascending albumin peak and alpha-globulin peak might be situated respectively above and below the flange plates between the upper and lower compartments.
    The compartments were then seprated by sliding over the plates, and albumin separated above the plates was taken out with along pipette. Repeating the same process, fractions were also prepared.
    Serologic actiivities of these fractions were determined by Wassermann, Meinicke and Murata tests.
    Concentrations of serum proteins in syphilitic serum were estimated on electrophoretic patterns, then compared with those of normal serum.
    Results and Discussion
    Results in Wassermann, Meinicke and Murata tests, are shown in Tables 1-3, and the relationship between these tests and the degree of serum dilution is shown in Table 4.
    Most of the fractions containing gamma-globulin indicate positive reaction in these tests. Therefore, it is considered that the antibodies in serologic tests for syphilis are contained in gamma-globulin.
    Even though beta-globulin may contin Wassermann antibody as Davis, et al described, since beta-globulin concentration in fraction is not high, the fraction may not indicate positive reaction.
    Table 5 shows that in sypholitic sera, an increase of gamma-globuling occurs, with some decreasee of albumin, and almost no quantitative change in alpha- or beta-globulins.
    It is observed that gamma-globulin increases remarkably, but the amount of beta-globulin does not change perceptibly.
    Therefore, even though beta-globulin may contain antibody, its amount is cosidered to be very small in comparison with that in gamma-globulin.
    1) Wassermann antibody is chiefly contained in gamma-globulin.
    2) The antibodies in Meinicke and Murata tests are closely related to Wassermann antibody, and are also chiefly contained in gamma-globulin.
    3) In syphilitic sera are found an increase of gamma-globulin, some decrease of albumin, and almost no quantitative change of alpha- and beta-globulins.
    抄録全体を表示
  • 金上 晴夫, 榊 喜代治
    1 巻 (1951 - 1953) 1 号 p. 39-40,46
    公開日: 2009/03/31
    ジャーナル フリー
    We designed the “Serogram” as a method to express the serum protein components. Since the previous year, we have been observing the variation of serum protein components by “Serogram” in the cases of tuberculosis treated with chemotherapeutic agents or by surgical means. The serogram of some diseases, such as carcinoma, nephrosis, kala-azar, etc., was found to show very specific figures, so that we assumed that serogram will be valuable as a method, of differentiating some diseases, and in expressing the condition of serum proteins easily.
    抄録全体を表示
  • 菅野 浩
    1 巻 (1951 - 1953) 1 号 p. 41-46
    公開日: 2009/03/31
    ジャーナル フリー
    The electrophoretic patterns of egg white under the condition of pH 7.8, 0.15μ (phosphate buffer) were obttined by employing the Tiselius apparatus with a cylindrical-lens system constructed by the author.
    The eleven components, except ovomucin, were reported and discussed by referring to the previous reports. The electrophoretic composition was computed from the patterns and compared with the results of Longsworth, et al. (1940), Bain, et al (1947), Forsyth, et al (1950), and Sørensen (1933-35).
    There was almost no difference between the three separate layers of egg white.
    Next, each process of the fractionation of egg white be sodium sulfate according to Kekwick and Cannan was traced electrophoretically. When sodium sulfate was added to egg white to half saturation, a precipitate containing mucin and globulins separated out. It was found electrophoretically, that this precipitate contained an especially large amount of G2-globulin, but a small amount of each globulin remained in the filtrate, Then, almost all of ovalbumin, with a small amount of conalbumin and ovomucoid, was precipitated by the saturation of sodium sulfate. A small amount of each globulin was also precipitated together. When this precipitate was dissolved into distilled watet, the remaining globulins were removed, and ovalbumin was recrystallized by the addition of sodium sulfate.
    The initially recrystallized albumin was almost pure, electrophoretically. When three recrystallization were repeated, aquite pure ovalbumin was obtained as needle crystals (20-30μ).
    抄録全体を表示
  • 吉澤 久雄
    1 巻 (1951 - 1953) 1 号 p. 47-51
    公開日: 2009/03/31
    ジャーナル フリー
    To calrify the presence of a relationship between erythrocyte sedimentation rate and plasma proteins in pulumonary tuberculosis, determination of plasma protein fraction of 75 patients was carried out by the electrophoretic method, extending to a total of 153 times.
    The results obtained are as follows:
    1) Erythrocyte sedimentation rates are significantly correlative with albumin, total globulin, fibrinogen and γ-globulin, and are not correlative with total plasma protein.
    2) α-Globulin and β-globulin are not correlative with erythrocyte sedimentation rates, but they are significantly correlative with common logarithm of erythrocyte sedimentation rates.
    3) It is presumed that erythrocyte sedimentation rates are determined by the combination of relative plasma protein fractions.
    抄録全体を表示
  • 1 巻 (1951 - 1953) 1 号 p. 55-63
    公開日: 2009/03/31
    ジャーナル フリー
  • 1 巻 (1951 - 1953) 1 号 p. 64-79
    公開日: 2009/03/31
    ジャーナル フリー
    • |<
    • <
    • 1
    • >
    • >|
feedback
Top