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Volume 39 (1998)
- Issue 6 Pages 345-
- Issue 5 Pages 297-
- Issue 4 Pages 241-
- Issue 3 Pages 165-
- Issue 2 Pages 61-
- Issue 1 Pages 1-
Volume 39, Issue 5
Displaying 1-18 of 18 articles from this issue
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Yoko KAWASAKI, Chiaki KATO, Hajimu ISHIWATA, Takashi YAMADA1998 Volume 39 Issue 5 Pages 297-302_1
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAn analytical method for the determination of sodium alginate in various foods was developed. Pectin and protein were removed from foods with 1.5mol/L sulfuric acid and 1mol/L magnesium sulfate-2.2mol/L sodium sulfate solution. Pectic substances in foods were removed as a precipitate with 1% sodium hydrogen carbonate solution. Alginic acid in the extract was precipitated as a copper salt, dissolved in 1mol/L ammonia water, and determined colorimetrically by means of a naphthoresorcinol reaction. The described method was applied to 18 kinds of food. The recovery of sodium alginate spiked at the level of 0.1% was more than 75%, except for soybean flour. The determination limit of the method was 0.002%. Sodium alginate was not detected in 18 kinds of food, but was detected in whipped cream, pudding and noodles at levels of 0.280%, 0.112% and 0.290%, respectively.View full abstractDownload PDF (727K) -
Yumi AKIYAMA, Naoki YOSHIOKA, Masahiko TSUJI1998 Volume 39 Issue 5 Pages 303-309_1
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSIn order to apply the multiresidue screening method to degradation products of N-trihalomethylthio fungicides (captan, captafol, folpet, dichlofluanid, tolylfluanid) and oxidized organophosphorus insecticides generated during sample preparation or in plants, the recovery of sixteen of these degradation products and metabolites from 3 agricultural products was investigated. The recoveries ranged from 74 to 129%, and these data suggest that the degradation products can be determined simultaneously with the parent pesticides by this method.
The degradation ratios of 5 N-trihalomethylthio fungicides, demeton-S-methyl and isofenphos were examined during sample preparation from 5 agricultural products and during GC injection. Cabbage, spinach and pumpkin readily caused the degradation of N-trihalomethylthio fungicides, and the degradation ratios of captan were 100, 100 and 81%, respectively. Decreases of the parent pesticides corresponded closely to the formation of the degradation products.View full abstractDownload PDF (840K) -
Yoko KAWAMURA, Kyoko NISHI, Harumi SASAKI, Takashi YAMADA1998 Volume 39 Issue 5 Pages 310-314_1
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA determination method was developed for styrene dimers and trimers in instant noodles contained in polystyrene cups. Soup and drained water were extracted by shaking with n-hexane, and the noodles were extracted by sonicating with n-hexane. Samples containing both noodles and soup were extracted by a combination of both methods. The extracts were cleaned by partition with acetonitrile and then eluted through a Florisil column. The styrene dimers and trimers were determined by GC-FID using 1, 3-diphenylpropane and benzyl n-butyl phthalate as standards. The recoveries of two dimers and four trimers from spiked samples ranged from 77.5% to 111.7%. The quantitation limits were 5ppb in the samples. The migration test was carried out using eight instant noodle samples and the migration levels of the styrene trimers in the noodles were ND-62ppb, while styrene dimers were not detected.View full abstractDownload PDF (624K) -
Michiko MIYAHARA, Hirotaka KONUMA1998 Volume 39 Issue 5 Pages 315-317_1
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSPCR was inhibited by raw meat containing blood. When raw meat was tested in broth culture at the 10% rate, the PCR system could not detect vero toxin gene of 5×107 cfu (colony forming unit)/mL of O157. PCR was also inhibited by meat extract, when the extract was mixed with the PCR sample. A DNA purification procedure which eliminates blood components is essential before PCR is conducted.View full abstractDownload PDF (2259K) -
Ayumu NAGAHARA, Satoshi FUKUDA, Mamoru KIKUCHI, Shizunobu IGIMI, Hideo ...1998 Volume 39 Issue 5 Pages 318-323
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSEgg yolk immunogloblins (IgY) which react specifically with staphylococcal protein A were studied to assess their suitability for use in an enzyme-linked immunosorbent assay (ELISA). The ELISA was positive for all 31 Staphylococcus aureus strains and was negative for 47 out of 49 (96%) other staphylococcal species. The ELISA was also performed for the detection of protein A added to homogenates of a rice ball and a cake, and these homogenates showed little inhibitory effect on the ELISA. The detection limits of the ELISA for enrichment cultures of S. aureus SA106 in artificially contaminated rice ball and cake were 7.6×102cfu/g and 5.5cfu/g, respectively. The ELISA test using anti-protein A IgY was found to be a simple method for detecting S. aureus.View full abstractDownload PDF (718K) -
Tsuneo HASHIMOTO, Toshihiro NAGAYAMA, Maki KOBAYASHI, Nahoko HANEISHI, ...1998 Volume 39 Issue 5 Pages 324-328_1
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSAn analytical method for determination of sulfonylurea herbicides, chlorimuron ethyl (CE) and tribenuron methyl (TM), in crops by HPLC was developed.
The sample was homogenized with acetone followed by extraction with ethyl acetate, and the extract was evaporated. The residue was defatted by acetonitrile-n-hexane partitioning for soybean, peanut and corn grits. The residue was cleaned up with a Florisil column and a Bond Elut® SAX cartrigde column. CE and TM were separated on an HPLC column (Inertsil ODS-2) using acetonitrile-water-phosphoric acid (50:50:0.01) as the mobile phase. These compounds were detected at 240nm.
The mean recoveries of these compounds added to crops at the level of 0.1ppm were 59-84% (CE) and 54-79%, except for peanut (TM). The detection limits of CE and TM in crops were 0.01ppm.View full abstractDownload PDF (640K) -
Minoru NAGAO, Takamitsu TSUKAHARA, Sasi JAROENPOJ, Chusak ARDSOONGNEAR ...1998 Volume 39 Issue 5 Pages 329-332_1
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA simple and sensitive method has been developed for the simultaneous determination of six new quinolones (benofloxacin, danofloxacin, difloxacin, enrofloxacin, ofloxacin and orbifloxacin) in beef, pork and chicken.
The drugs were extracted from the meat (1.0g) with 0.3% metaphosphoric acid (2mL) and acetonitrile (20mL). The supernatant was concentrated to dryness after having been defatted with n-hexane (10mL). The residue was dissolved in 5mL of HPLC mobile phase and analyzed by HPLC.
The HPLC analysis was carried out on a TSK-gel ODS 80Ts column (4.6×250mm) with 0.05mol/L phosphate buffer (pH 2.5)-acetonitrile (4:1) containing 0.25g/L sodium octanesulfonate as the mobile phase at a flow rate of 1.0mL/min. A spectrofluorometric detector was operated at Ex 290nm and Em 460nm. The recoveries of six drugs from fortified meat were greater than 80% and the detection limits were in the range of 0.0025-0.05μg/g.
This method is concluded to be useful as a simultaneous analytical method for new quinolones in meats.View full abstractDownload PDF (442K) -
Hirotaka KAKITA, Takao KITAMURA, Katsuo KOMIYA, Yoshio KATO1998 Volume 39 Issue 5 Pages 333-340_1
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSA rapid and highly sensitive method has been developed for the simultaneous determination of reducing monosaccharides and oligosaccharides by normal-phase HPLC with fluore-scence detection. The technique of linear gradient elution on a TSKgel Amide-80 column was more suitable for saccharides separation. The post-column reaction was optimized for fluorometric detection. Under optimum conditions, the detection limits were 0.3-15ng for the reducing saccharides investigated. The calibration curves were approximately linear in the range of 2, 000-12.5pmol for glucose, cellotriose, and cellopentaose. The coefficient of variation for glucose was less than 1%. Application of the method for food analysis was successful.View full abstractDownload PDF (893K) -
Susumu ISHIMITSU, Ikuko MISHIMA, Sumiko TSUJI, Tadashi SHIBATA1998 Volume 39 Issue 5 Pages 341-344_1
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
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[in Japanese]1998 Volume 39 Issue 5 Pages J390-J391
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
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[in Japanese]1998 Volume 39 Issue 5 Pages J391
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
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[in Japanese]1998 Volume 39 Issue 5 Pages J392-J393
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
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[in Japanese]1998 Volume 39 Issue 5 Pages J393-J394
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
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[in Japanese]1998 Volume 39 Issue 5 Pages J394-J395
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
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[in Japanese]1998 Volume 39 Issue 5 Pages J395-J396
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
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[in Japanese]1998 Volume 39 Issue 5 Pages J396-J397
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
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[in Japanese]1998 Volume 39 Issue 5 Pages J397-J398
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
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[in Japanese]1998 Volume 39 Issue 5 Pages J398-J399
Published: October 05, 1998
Released on J-STAGE: December 11, 2009
JOURNAL FREE ACCESSDownload PDF (307K)
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