関西医科大学雑誌

Dosage Dependent Suppression of a crp - Mutant by yciM Gene Product in Escherichia coli

Satoshi Mizutani, Aiko Umemoto, Farjana Jahan Fattah, Kazi Riaz Fattah ...

2002 年 54 巻 2-4 号 p. 119-123
発行日: 2002/12/30
公開日: 2013/02/19

DOIhttps://doi.org/10.5361/jkmu1956.54.2-4_119

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yciM, one of the uncharacterized ORFs in Escherichia coli, located next to the DNA replication terminus terA, was found to suppress a suppressor sensitive crp- mutant in 2 copies, one placed in trans in a single copy plasmid and the other on the chromosome in cis, but not in single copy on the chromosome. Moreover, it seems to be lethal to the host in multiple copies, because it could not be subcloned in a multicopy vector. The suppression sensitive crp mutation was leaky and had been credted by the insertion of an 152 element between the crp promoter and its coding region.

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Changes in cortical field potentials in learning processes of audio-initiated vocalization and influences of cerebellectomy upon the vocalization in the monkey

Hisae Gemba, Shin-ichi Kyuhou, Ryuichi Matsuzaki, Yae Amino, Kazuko Ma ...

2002 年 54 巻 2-4 号 p. 124-129
発行日: 2002/12/30
公開日: 2013/02/19

DOIhttps://doi.org/10.5361/jkmu1956.54.2-4_124

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Recording field potentials during learning processes of audio-initiated vocalization by electrodes implanted chronically on the surface and at 2.0-3.0 mm depth of the cerebral cortex and analyzing them seem useful for knowing central nervous mechanisms of the vocalization in the monkey. We have been waiting for an opportunity to record the potentials during the motor learning in a short period, because it usually takes a considerable time (3-6 months) for a naive monkey to be trained well for the vocalization. One of three monkeys, which had been fully trained for the vocalization, was found to be unable to perform the vocalization after electrode-implanting operation. We could record the potentials in the monkey during the process of learning the vocalization again. The learning process of the vocalization partly differed from audio-initiated hand movements previously reported, and there was no skill learning in the vocalization. The right cerebellar hemispherectomy eliminated the surface-negative, depth-positive (s-N, d-P) premovement potential recorded before the operation in the left face motor cortices in the three monkeys, and changed vocal tone. Scarce change was seen in reaction times in audio-initiated vocalizations before and after the operation, again differing from audio-initiated hand movements. This suggests that central nervous mechanisms in audio-initiated vocalizations differ from those in audio-initiated hand movements. These nervous mechanisms were discussed in connection with the limbic system.

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Our Experience of Muscle Afferent Block for dystonia and spasticity

Hisashi Ito, Satoshi Nakano, Hidefumi Ito, Hirofumi Kusaka

2002 年 54 巻 2-4 号 p. 130-134
発行日: 2002/12/30
公開日: 2013/02/19

DOIhttps://doi.org/10.5361/jkmu1956.54.2-4_130

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Muscle Afferent Block (MAB) has been shown to improve clinical symptoms of writer's cramp, cervical dystonia, spastic paraparesis, and oromandibular dystonia. However, this therapy is not yet used to a sufficient extent. In this article, we review our experience with this new treatment The subjects were 11 cases encountered between November,2001 and April,2002, including 4 cases of focal dystonia of an upper limb,3 cases of spastic paralysis of an upper limb,3cases of spastic paraparesis, and 1 case of spasmodic torticollis. All patients exhibited some clinical improvement. Two cases of facial flushing and 3 cases of muscle weakness occurred as side effects. All of them were mild and transient. We conclude that MAB is a useful treatment for dystonia and spasticity with low incidence of side effects.

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関西医科大学医学会賞

日置絃士郎

2002 年 54 巻 2-4 号 p. 135
発行日: 2002/12/30
公開日: 2013/02/19

DOIhttps://doi.org/10.5361/jkmu1956.54.2-4_135

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「関西医科大学医学会賞」は関西医科大学医師会の後援のもと,当該年本学で学位を授与された若手研究者を鼓舞し,更なる飛躍を期待するために設けられた賞である。

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powerful new strategy for allogeneic bone marrow transplantation in mice

Taketoshi Kushida, Muneo Inaba, Hiroko Hisha, Naoya Ichioka, Takashi E ...

2002 年 54 巻 2-4 号 p. 136-149
発行日: 2002/12/30
公開日: 2013/02/19

DOIhttps://doi.org/10.5361/jkmu1956.54.2-4_136

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We have succeeded in treating intractable autoimmune diseases in chimeric resistant MRL/lpr mice by a new bone marrow transplantation (BMT) method consisting of fractionated irradiation (5.5 Gy x2) followed by intra-bone marrow (IBM) injection of whole bone marrow cells (BMCs) from allogeneic normal C 57 BL/6 (B 6) mice [5.5 Gy X2+ IBM]. In MRL/Ipr mice treated with this method, the number of not only donor-derived cells but also donor-derived hemopoietic progenitor cells increased. Furthermore, donor-derived stromal cells were clearly detected in the cultured bone pieces from MRL/lpr mice treated with [5.5 GyX2+IBM]. All the recipients thus treated survived more than one year (60 weeks after birth) and remained free from autoimmune diseases. Autoantibodies decreased to almost normal levels, and abnormal T cells (Thy 1.2+/B 220k+CD 4-/CD 8-) disappeared. These findings clearly indicate that this new strategy (IBM-BMT) creates the appropriate hemopoietic environment for the early recovery of hemopoiesis and donor cell engraftment, resulting in the complete amelioration of intractable autoimmune diseases in chimeric resistant MRL/lpr mice without recourse to immunosuppressants. This strategy would therefore be applicable to human therapy.

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新生仔マウス心におけるIsK(minK)アンチセンスオリゴヌクレオチドおよびD77NlsK変異体過剰発現による心筋遅延整流カリウムチャネルの抑制

大山秀夫

2002 年 54 巻 2-4 号 p. 150-156
発行日: 2002/12/30
公開日: 2013/02/19

DOIhttps://doi.org/10.5361/jkmu1956.54.2-4_150

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原発性シェーグレン症候群患者の末梢血樹状細胞に関する検討

吉郎尾崎

2002 年 54 巻 2-4 号 p. 157-163
発行日: 2002/12/30
公開日: 2013/02/19

DOIhttps://doi.org/10.5361/jkmu1956.54.2-4_157

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シェーグレン症候群(SS)は唾液腺・涙腺といった外分泌腺が障害の中心となる自己免疫性疾患であり,その発症機序は依然不明である.近年monocyte-derived DC(dendritic cell;樹状細胞)がnaiveTcellをType-1 helper T cell(Th1)に,plasmacytoid DCがTh2に分化誘導することが報告され,DCが免疫系の制御に中心的な役割を果たすことが理解されてきた.我々は,ヒト末梢血中に3つのDC亜群を見い出し報告した(fraction 1;CDlc+/CDllc+,fraction2;CDlc-/CDllc+,fraction 3;CD 1 c-/CD 11 c-).前2者は,いわゆるmyeroid DCであり,fraction 3はplasmacytoid DCである.23名のprimarySS(pSS)と健常者の末梢血DCを検討したところ,pSSではfraCtion 1が著明に減少していた.また,小唾液腺生検組織に対するDC特異的抗原であるfascinを用いた免疫染色で,facsin+/HLA-DR+細胞が浸潤単核球巣中に認められた.末梢血Tcel1のintracellular stainingでは,IFNγ 産生T cellの増加がpSS患者において認められると共に,fraction l DCがnaive T ce11をTh 1に誘導することがfraction l DCとnaive T cellとのallogeneicco-cultUreで示された.また,小唾液腺組織に於いてもIFNγ 産生CD4細胞が優位に浸潤してた,以上のことからpSSでは,組織にfraction 1 DCが動員され,局所でのcytokine balance をTh1にdriveしていることが,その発症機序の一因として示唆された.

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ラムドイド・ファージ・ゲノムの比較

ベロ毒素遺伝子との関連

杉野義信

2002 年 54 巻 2-4 号 p. 164-176
発行日: 2002/12/30
公開日: 2013/02/19

DOIhttps://doi.org/10.5361/jkmu1956.54.2-4_164

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1)ベロ毒素遺伝子はラムドイド・ファージ・ゲノムの特定の位置にファージ特有の制御を受けつつ存在し,従って,今まで明らかになった範囲では,ファージ自身の遺伝子であって,細菌の染色体本体から由来したものと考える根拠はない.2)ラムドイド・ファージでは,それぞれの蛋白質とそのDNA上の作用点とについて,特異的な要素が対をなして存在し,その蛋白の遺伝子とターゲットとの組は組換えの前後も不変に保たれる.離ればなれになると特異性があわないために作用が失われる.3)ラムドイド・ファージはゲノムとしては極めて小さいので,ゲノム比較分析の方法を洗錬しテストするために極めて適した材料である.

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