In previous reports, we could revealed that the metastatic incidence of tumor bearing animals following surgery under hypothermia was significantly enhanced, The purpose of this reports is to clarify the mechanisms respon sible for the augmentation of metastatic incidence in tumor bearing animals following hypothermia As a result, we could reveal that hypothermia caused the mutual adhesiveness of tumor cells to dissociate, and a tendency of tumorcells to stick more easily to vessel wessel walls. We have demonstrated that the incidence of tumor metastases following hypothermia may be augmented by increased numbers of circulating tumor cells, by alteration of host factors such as hypercoagulability and reticuloendothelial interference. In addition, alteration of blood oxygen concentration influence the development of metastases.
1. Non-specific hyaluronidase inhibitor in the rabbit serum increased in both cases of bacterial and non-bacterial infections, and the values of inhibitor related to the degree of general emaciation rather than to that of inflammatory reactions. 2. This fact suggests that the increasing of the inhibitor was caused by tissue destruction. 3. Heparin inhibited hyaluronidase activity in vitro, however heparin adding serum did not show this inhibitory effect. In vivo, too, heparin showed no inhibi t i on to the enzyme.
Potassium and sodium concentrations (mEq/l) in the anterior tibial muscles of rats were measured by the wet method with the aid of a flame photometer. The right anterior tibial muscle of the rats had been denervated and the left had been left intact. After denervation, the K-concentrations in the muscles decreased and the Na-concentrations increased. When the muscles were in situ dialysed after the intraperitoneal administration of K-free Tyrode's solution in a total dosage of half body weight, the K-concentrations in the muscles further decreased and the Na-concentrations increased. After the K-dialysis in situ, a KCl-solution in a dosage of 50mg/kg was intraperitoneally injected. The KCl-injection increased the K-concentrations in the muscles and decreased the Na-concentration. Drugs effects were examined on the K-and Na-movemet in these muscles when administered 30 minutes before the KCl-injection. l-Methionine (100mg/kg, i. p. )was found to promote K-influx and Na-efflux. On the other hand, strophanthin-G (5-μg /kg, i. p. ), adrenaline (0.1 mg/kg, s. c. ), Spa and d-Spa (l-and d-1,2-diphenyl -1-dimethylaminoethane 15mg/kg, i. p. ) procaine amide (20mg/kg, i. p. ) and insulin (5 units/kg, s. c. ) inhibited K-influx and Na-efflux.
The pigment and fluorescent substances produced by Pseudomonas aeruginosa, strain No.134, in glutamic acid liquid medium were fractionated by gel filtration, and the presence of pyocyanin and of the snbstances corresponding to the Fluorescence I and II were demonstrated. The apsorbtion spectrum of fluorescence I of strain No.134 shows a peak at 460mμ. A reversible change in the fluorescent color of Fluorescence I fraction takes place between pH 5.0 and pH 10.0. A remarkable change is noted in the absorption spectrum as well as characteristic wave length at about pHs 5.0∼4.0 when the tone of the fluorescence changes unreversibly from colorless to orange. The paper electrophoretic analysis was perform ed on the Fluorescence I fraction of strains No.134 grown in the glutamic acid lipuid medium and three fluorescent spots were demonstrated. These spots appear in oder from the negative pole side with time of cultivation. The fluorescence II fraction shows seven fluorescent spots on the filter paper by electrophoresis. Three of them were purely isolated by starch zone electrophoresis and examined spectrophotometrically.
When the rabbit atria arrested in vitro by reserpine 3×10-5 (g/ml) were kept in the reserpine containing Locke's solution for 60 to 90 minutes, the atrial contractions did not resume spontaneously even after bath fluid was repeatedly changed. Thus reserpine treated atria could be resumed by l-, d- or ld-methionine 10-3, Catron 10-5, strophanthin-G 10-6, ephedrine 10-4 or tyramine10-4. However, in the case of the atria excised from rabbit pretreated with reserpine 1mg/kg these drugs could not resume the atrial contractions arrested by the above described reserpine (3×10-5) action. The stimulant actions of nicotine 10-5 to 10-4 on the normal atria were potentiated in the presence of l-, d- or ld-methionine 10-4 or strophanthin-G 10-6 but depresseds in the presence of ephedrine 10-5 or tyramine 10-6. The atrial contractions were augmented by l-, d- or ld- meth ionine 10-3, strophanthin-G 10-6, ephedrine 10-6 or tyramine 10-6. The atria excised from rabbits pretreated with reserpine 1 mg/kg did not respond to tyramine in any concentration, and the effects of ephedrine 10-5 on these atria were weaker than that on the normal atria. l-Ethion ine 10-4 to 10-3, glutathione 10-4 as well as l-cysteine 10-4 showed no remarkable effect on the normal atrial contractions, while l-ethionine 10-4 antagonized to the stimulant action of l-methionine 10-4 to 10-3 on the normal atria. From these experimental results, it was assumed that methionine, stro phanthin-G, ephedrine or tyramine may affect the active mobilization of endogenous catecholamine in atria and that ephedrine or tyramine may stand in catecholamine receptors to compete with endogenous catecholamine, and it was discussed that ephedrine tachyphylaxis may be developed from the endogenous catecholamine-blocking action of ephedrine binding to the catecholamine receptors.
Aortic arch syndrome is defined as an absence or diminution of pulses in the arms and neck by the cause of obstruction or narrowing of the branches of aortic arch; and then it is of multiple etiology, due to different causes: arteritis, atherosclerosis, aortic syphilis with or without aneurysm, aneurysm of the aorta, trauma to the aortic arch and congenital anomalies. However, most cases with this syndrome can be divided into two main groups, i. e., those of non-specific arteritic origin (pulseles disease) and of atheromatous origin. In this report, we present a 19 year- old female of typical pulseless disease, and a 65 year-old woman with obstruction of the right common carotid and subclavian arteries by atherosclerosis. We make clear the relationship in the concept of these diseases as undertaking the consideration on the literature and show the arterial reconstructive operation with success in the treatment of the aortic arch syndrome.