In the preceeding paper, the authors have found the rapid incorporation of inorganic phosphate absorbed through lear epidermis into some organic combinations including acid soluble-barium soluble phosphorus compounds primarily. In the present paper, resting on the basis of fine separation of phosphorus compounds in the use of paper-chromatography, the discussion on the forms of transferring phosphate after the foliar absorption are made. Thus one of the leaves of shakshina (Brassica chinensis L.) grow in field, about 30cm high, was applied with ca 1.5ml. of 1/75 M potassium dihydrogen phosphate containing P-32 in the strengh of 5 micro curie/ml. on a fine day. After 4 hours of this application, the poetiole was cut, and the exudation from this cut end was used for paper-chromatography as it was recommended by R. S. BANDURSKI and B. AXELROD. The developed paper strip was examined for its phosphorus spots by spraying HANES-ISHERWOOD reagent and its radio-activity by means of Geiger-Muller counter as it was reported by R. M. TOMARELI and K. FLOREY. Obtained paper-chramatograms and radiograms are shown in Fig. 1 and 2. Compared with Rf value of each particular known phosphorus compounds that had been determined in the same condition as that employed in the experimental procedure, transferring chief phosphorus compounds which have been newly synthesized in leaves are estimated to be confined to a few other compounds than any of ortho-phosphoric acid, glucose-1-phosphate, and fructose-6-phosphate. Under the condition which have been made, the sum of radio ortho-phosphoric acid, glucose-1-phosphate, and fructose-6-phosphate in the exudate is concieved less than 10% of total radio phosphorus in the exudate. S.ARONOFF has shown that the primary products of phosphate assimilation by soybean roots is fructose-1,6-diphosphate together with some phosphorylated organic acids, and also in our experiment, inorganic phosphate absorbed through leaf-surface is illustrated to be easily assimilated in leaf tissue and converted to such products.

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1) Some preliminary experiments were conducted with the object of knowing the condition of high productivity of paddy field scattered in the plain of Nakatado-gun, Kagawa-prefecture. 2) It was observed that the development of rice plant roots was better in highly productive fields than that a degraded fields. Concerning the relation between the amount of nitrogen supplied and the development of rice plant roots, dressing with more nitrogen did not necessarily mean better root development in the case of a paddy field of the Hiroshima Agricultural Experimental Station where the soil degraded, but in the case of a paddy field of the Shikoku Agricultural Experiment Station, where dressing with more nitrogen annually resulted in considerable increase in yield, the development of rice plant roots was greater in proportion to the amout of nitrogen increased, which fact is, in authur's opinion, a peculiarity of highly productive paddy field. 3) As to the results of measurment of base exchange capacity of rice plant root; the base exchange capacity of rice plant root in each layer of soil columns of highly productive paddy fields proved greater than that of degraded paddy fields. Again in the case of a paddy field of the Hiroshima Agaicultural Experiment Station, the amount of the supplied nitrogen did not noticeably changed base exchange capacity of rice plant roots, but in the case of a paddy field of the Shikoku Agricultural Experiment Station, which have a peculiarity of of yielding better crops, base exchange capacity of rice plant roots evidently increased in propotion to the amount of nitrogen increased.

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After their morphological characters, paddy field soils were classified into three types, Half-bog soils, Meadow soils, and Gray lowland soils ; the types being subdivided into soil kinds, ten in total. (see Table 1 & 2; & Map) Of each soil type, some suggestions or remarks were made to increase the productivity in the follwing way. (Half-bog soil) : Drainage is, first of all, required. When completed, the drainage may be feared to cause soil nitrogen to become excessively available for the plant to an extent to hinder the normal growth of plant. Heavy application of phosphate will be rewarded. Coating with soils, if possible, will be appreciated. (Meadow soil) : Drainage is required except types of hilly area. In order to keep applied nitrogen from its release from surface of the field in a free gas from, nitrogenous fertilizers must be applied as deep as they are kept in reduced condition. Of sandy soil kind, fertilization must be made separately in suitable seasons, lest it should cause the constituents to be leached away. Physiological decay of plant roots, occasionally cbserved, will be avoided by applying fertilizers of non-sulphuric acid radical. (Gray lowland soil) : Deep cultivation will be appreciated. Heavy application of farm yard manure is required. When used as a dry farm, the acid reaction of soil, expected to easily occur, must be neutralized. Among the types, listed in this report, this soil type may be considered to be the most profitable.

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By the use of measuring tube with a glass filter plate on its bottom, soil volume in water was measured at various tensions, attained about 90 cm of water in height. Results obtained were as follows : 1. Settling volume of soil in water was decreased when water was percolated through the soil column, and was more decreased by the application of tension. 2. The more the soil had clay, the more the shrinkage of the soil volume was remarkable. 3. High correlation coefficient was found between the clay content and the settling volume in water, but the coefficient turned to be lower by the tension. 4. It was supposed that there produced a negative pressure in the soil column in water, when water was percolated through the soil of having both pores, small and large. 5. The formation of so-called plow pan in paddy soil and also the shrinkage of certain volcanic ash paddy soil, occurs several years after reclamation, were both concluded to be caused to the nagative pressure produced as in 4.

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Rice plants are supplied at the beginning of the shooting with (N^<15>H_4)_2SO_4 and various parts of brown rice, that is, the embryo, the bran and the proteins of the endosperm, are isolated and analyzed for their isotope concentration. In all parts, the N^<15> concentration of the embryo is the highest, and next come that of globulin, oryzenin and insoluble protein in the endosperm. Next in order of decreasing N^<15> concentration comes the bran. And finally, it should be emhasized that N^<15> concentration of prolamine and albumin in the endosperm are lower than that of any other parts. Whether the differences in the isotope concentration of various parts indicate the relative physiological importance of the respective parts or not, and also whether those differences are influenced by the mechanism of maturing process or not must be regarded as open questions.

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By circular and paper chromatography four adsorption bands giving their fluorescence by being illuminated with ultra-violet ray were observed in both A and B type of humic acids isolated from black soil, humus volcanic ash soil and low moor peat. But only three bands were observed in the case of brown humic acid from high moor peat. Concerning the substance isolated from each separated band the Rf value and the distinction coefficient were determined and in view of the results thus determined it seems that both A and B type of humic acids undoubtedly contain three or four fundamental components.

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Stramonium, eggplant and their reciprocally grafted plants were cultivated under solution culture in order to see the effect of both rootstock and scion on the yield, nutrient absorption, inorganic and alkaloid content of the grafted plants. Results are summarizes as follows : 1. The yield of the scion was remarkably affected by the rootstock, and on the other hand that of the rootstock seemed to be influenced by the scion. 2. Amounts of water and nutrients (NO_3,HN_4,K_2O, P_2O_5) absorbed for a few days by the grafted plants measured at their early stage of growth were largely affected by the rootstock, and moreover, the ratio of absorbed NO_3-N/NH_4-N was also regulated by the rootstock. 3. Total ash. K_2O and C_aO contents (%) in leaves and stems of the grafted plants seemed to be regulated by the scion, but such constituents as P_2O_5 were largely influenced by the rootstock. 4. Total alkaloid content of the scion seemed to be affected by the rootstock.

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Tomato plant absorbs and accumulates aluminium readily from aluminium solution which contains AlCl_3 (Al 4.2ppm) at pH5.2. The Al amounts absorded in 2 days differ significantly with the composition of nutrients solution as fullows : [chemical formula][table]

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Colloid chemical studies were pursued with the Taizanjino uncultivated top-soils which had been incubated for a conciderable time with organic material and calcium. In orignal-and not incubated top-soils, rich in humus, rotted products were hastened to be composed by drying. By this drying the soil seemed to be brought to have a min-value in pH-ζ curve. On the other hand, where incubated in the above way, the soils could have a min.-value in pH-ζ curve. We supposed that the created good humus gives these properties of matured soil to the incubated soils.

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Experiments were carried out in order to determine maximum and optimum temperature as well as thermal death point for rhyzobia. Although the maximum temperature for most of rhizobia lies between 32°and 37℃., yet strains of alfalfa, Sesbania, and one of cowpea bacteria areable to grow at 39°to 42℃. The strains for which 32℃. is maximum are found in those from Indigofera, Styphnolobium, lupine-, soybean-, and cowpea-group, while the strains for which 37℃. is maximum are those from Mimosa, Leucoena, Dalea, and Vicia(nipponica), and some of pea-, clover-, and bean-group. The maximum temperature for fast-growers from pea-, clover-, and bean-group are somewhat higher than those for slow growers from lupine-, soybean-, and cowpea-group. The grouping of strains seems to be possible, depending upon maximum temperature, in each of Sesbania, alfalfa-, soybean-, and cowpea-group, respectively. The optimum temperature for alfalfa-, and Sesbania bacteria is about 34℃. The exposure for ten min. at 60℃ or thirty min. at 55℃ is necessary for the complete death of rhizobia, though there are many strains which are killed by the treatment for ten min. at 55°or thirty min. at 50℃.

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The same materials of the previous work (part 1), i.e. leaves and stems of the soybean plants which grew on the plots of the Mg-deficient soil fertilized with different, amounts of MgSO_4 and were harvested two week intervals, were used. These materials were divided into the fractions solved in such soluvents as distilled water, 2% acetic acid and 10% hydrochloric acid, and the insoluble one. The amounts of Ca in each fraction were determined. The results obtained may be summarized as follows; (1) Stems of the Mg-deficient soybean plants contain CH_3COOH soluble Ca more abandantly than those of the normal plants throughout the whole stages of growth. Leaves of the Mg-deficient soybean plants also contain CH_3COOH soluble Ca more than the normal plants. But at the podding stage, the amount of CH_3COOH soluble Ca in them decreased suddenly and became less than that of the normal plants. (2) These results may be explained as follows : (A) In the Mg-deficient soybean plants the pectine substance in middle lamella of cell wall may need Ca to substitute for Mg. As much Mg is generally required for the formation of pods, Ca in leaves is considered to be transported to the pods instead of Mg in the Mg-deficient soy-bean plants. (B) As Ca-ion and Mg-ion act similarly to coagulate pectine substance, they can substitute to each other in leaf and stem and the amounts of Ca and Mg combining with pectine substance seem to have a relation to mechanical hardness in the plant structure and to disease-resistance.

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Relations between clay soil, property of which as shown in Table 1,soil water and base exchange capacity were studied. Amounts of Krillium added to soil were 0.01,0.05,0.1 and 0.5%, and those of soil water were 5,10,15,20 and 30%, corresponding to about 25,35,50,60 and 85% of water capacity respectively. By the addition of Krillium, capillary movement in the soil was hastened, when the soil contained water more than 15%, approximate value of hygroscopic coefficient in both systems, Ca and Na. Percolation of water through dry soil was retarded by the addition of Krillium, but it was hastened at 15% of water. Percolation of NH_4Cl solution was more retarded them that of water. Addition of Krillium caused both soil volume in water and base exchange capacity to be increased, while it caused water capacity to be decreased in Ca-system and to be increased in Na-system.

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