Light-weight nursery soil has been partially adopted in Hokkaido, Japan, for use in the sugar beet paper pot transplanting system. The nursery soil for sugar beet normally comprises mineral soil, ground rice chaffs moistened with water, and fertilizer. We investigated the effect of rice chaffs in nursery soil on the quality of paper pots after growing sugar beet seedlings, which should be strong enough for transplanting. The nursery soil containing rice chaffs comprised half alluvial soil, half ground rice chaffs with 15% (v/v) water, and fertilizer. Nursery soil with only the alluvial soil and same fertilizer and without rice chaffs was used as control.
1) After mixing, the nursery soils were incubated in a plastic bag for 50 days at 25°C. The paper pots were filled with the incubated soils and subsequently incubated at 25°C for 20 days with or without sugar beet seedlings. After incubating the paper pots, the paper quality of each pot was determined. The paper pots with nursery soil containing rice chaffs showed significantly lesser weight reduction, caused by microbial decomposition, and higher paper strength than the control nursery soil. However, the cellulase activity of the nursery soil containing rice chaffs was not significantly different from the control nursery soil.
For further investigation, the soil samples were obtained from either close to or further away from the paper. The soil placed close to the paper had a higher cellulase activity in the control nursery soil than in the nursery soil containing rice chaffs, suggesting that the localized higher cellulase activity induced a higher decomposition in the control nursery soil.
2) The nursery soil containing rice chaffs had lower NO3–N concentrations than the control nursery soil, suggesting that the decomposition of rice chaffs induced nitrogen competition among the microbial community.
3) Microbial carbon utilization patterns were investigated using the Biolog EcoPlate (Biolog, USA). The microbial diversity was higher in the nursery soil containing rice chaffs than in the control nursery soil. Further, the carbon utilization pattern detected using the EcoPlate showed that the presence of paper and seedling roots drastically changed the microbial community structure in the control nursery soil, but not in the nursery soil containing rice chaffs, suggesting that the microbial structure in the nursery soil containing rice chaffs was stable.
4) Because mixing of nursery soil with rice chaffs made the microbial community structure compete for nitrogen and become more stable, the localized enhancement of cellulase activity in the soil close to the paper was not induced.
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