牛,馬,山羊,豚,兎,ラット,マウス,モルモットの精子細胞,および牛,馬,豚の射出精子のacrosomicsystemを構成しているPAS陽成物質の細胞化学的性状にっいて実験を行ない,っぎのような結果をえた。
1.精子細胞のacrosomic systemのPAS陽性物質豚精子細胞のacrosomic systemのPAS陽性物質はグリコーゲンと酸性多糖類ではなく,固定にカルノア液を使用した場合に抽出除去され,脂肪溶媒に可溶性の物質であった。しかし,オルト液のような重クロム酸塩を含む固定液で固定を行なうと脂肪溶媒に不溶解性のPAS陽性物質に変化した。また,オスミウム酸で染まり,ペプシンおよびトリプシン消化によって変化しなかった。豚以外の動物については,脂肪溶媒に対する溶解性を検討したが,いずれも脂肪溶媒に溶解することが知られた。
したがって,精子細胞のacrosomic systemを構成するPAS陽性物質は従来いわれているような炭水化物•蛋白質複合体ではなく,脂質の一種,多分糖脂質であると考えられる。
2.射出精子のacrosomic systemのPAS陽性物質 牛射出精子のacrosomic systemを構成しているPAS陽性物質の細胞化学的性状は精子細胞の当該物質と同じであった。すなわち,グリコーゲンと酸性多糖類は検出されず,脂肪溶媒に溶解し,重クロム酸塩を含有する固定液によって脂肪溶媒に不溶解性の物質に変化した。ペプシン消化およびpH 2.2~11.3の緩衝液による前処理はPAS染色強度に影響をおよぼさなかった.また,馬と豚の射出精子を脂肪溶媒,すなわち,カルノア第2液で固定したところ,PAS陽性物質は完全に抽出除去された。これらのことから,射出精子のacrosomicsystem の PAS陽性物質はやはり精子細胞と同じ物質によって構成されていると考えられる。

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Pregnant mare serum gonadotropin (PMSG) was injected with the Freund's adjuvant into a rabbit, and induced the production of an anti-PMSG serum which showed precipitin and agglutinin properties. By means of this precipitin test, the anti-PMSG serum may be used for an immunological test for pregnancy. The antiserum also has antihormonal properties against the pregnant mare serum gonadotropin activity of the serum of pregnant mare.

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In our previous report, it has been demonstrated that the length of the estrous cycle of cattle can be shortened by inserting rubber tube into the uterine horn 1-3 days (early luteal phase) or 7-8 days (functional luteal phase) after ovulation. These results suggest that the uterus plays an important role in regulating estrous cycle length in cattle. The purpose of the work presented here is to investigate the effects of uterine distention by injecting a viscous gel-like substance into the uterus on the length of the estrous cycle in cattle. The fertility of the animal following the treatment was also investigated.
The experiments were performed on a total of 34 cycles of 9 cows including 7 Japanese beef cows and 2 Jersey cows. When an animal was used successively, she was retreated by uterine distention after it was confirmed that the previous treated cycle had been followed by 1-3 cycles of a normal length. The viscous gel-like substance “Gelceptor F” G had been prepared originally for treating bovine endometritis. It contains 500mg of Fradiomycin sulfate, 280mg of Bithionol and 10, 000 IU of vitamin A palmitate per 100g. Each 75g of G is kept in a plastic tube for practical use. It was injected into the uterus through the cervix by using a special injector which composed of a metal catheter and a screw driver for rolling up the tube containing G. Dose of G was 42-137g per injection. The day on which ovulation occurred was considered as day 0 of the cycle. The results obtained were as follows.
1) Experiment 1 (Preliminary experiment) : G was injected successively at intervals of 2-4 days. In all of 5 animals wich received 2 injections of G on day 2 and day 5 or 6 (early luteal phase), ovulation occurred 7-12 days after the first treatment. The mean length of the treated cycles was reduced to 11.9 days as shown in Fig. 1. Four animals were received 2-5 injection from day 12 to day 21 (functional luteal phase-late luteal phase). Two of the treated cycles were fairly lengthened, the cycle length being 28 days and 26 days respectively as shown in Fig. 2. The remaining 2 cycles were of a normal length. From these results, it was demonstrated that the estrous cycle could be modified by injecting this viscous gel-like substance into uterus.
2) Experiment 2: Single or double intrauterine treatments were performed at various stages of the estrous cycle to clarify the relation between the time of the treatment and the cycle length. The results were shown in Table 1. In 4 cycles treated on day 1 (postestrus), 1 cycle was reduced to 8 days, and the remaining 3 were of a normal length. In 5 cycles treated on day 2(early luteal phase), 4 cycles were shortened and the remaining 1 was of a normal length, the mean cycle length being 12.0 days. In 4 cycles treated on day 6 (early luteal phase), all of them were shortened, the mean cycle length being 15.5 days. In 4 cycles treated on day 12 (functional luteal phase), the mean cycle length was 21.3 days. It was not different from those of the previous and following cycles in these animals. All of 3 cycles treated on days 14 and 16 (late luteal phase) were somewhat lengthened, the mean cycle length being 24.7 days. In 5 cycles treated on day 17 or 18 (late luteal phase or proestrus), 3 cycles treared at late luteal phase were lengthened (one of them was observed to be abnormally long), and the remaining 2 cycles treated at proestrus were of a normal length.

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The rate of disappearance of HCG injected intravenously from the bloodstream was studied using adult ovariectomized mice and the rise and decline of this hormone in the bloodstream after subcutaneous injection was also investigated.
The results obtained were summarized as follows.
In the case of intravenous injection, the HCG activity in the blood decreased to 50% within 3 hours after injection, and after 24 hours it was under 6% as shown in Fig. 1.
After subcutaneous injection of HCG, it was observed that the HCG level in the blood increased to 6.25IU/6.25IU/2ml at the 6th hour and this value was maintained during the next 6 hours, then gradually decreased to be less than 2IU 24 hours after injection, as shown in Fig. 1.
The absorption, clearance, and fate of HCG were also discussed.

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