未成熟雌マウスに,連日, P.M.S.G.,C.G., 5i.u.を90日間注射した結果,次の成績を得た。
1) P. M. S. G., 5i. u. の連日注射マウスでは,第30日以降から, C. G., 5i. u. 連日射マウスでは,第60日以降から,アンチホルモンが生産された。
この抑制作用は,注射の継続によつて強化され, 90日目の検定では, P. M. S. G., C. G., 注射群の卵巣重量とコンロール群の卵巣重量と殆ど差がみられなかつた。
2) 下垂体前葉のBasophile cellは,アンチホルモンの生産と共に増加する傾向がみられた。
3) P. M. S. G., C. G. 両注射群の60, 90日検定では肝臓肥大がみられた。又, Vaginal Smearでの発情兆候は,アンチホルモン生産と比例するものと思われる。
本研究に実施に当つて御指導戴いた東大小林教授,新谷博士並びに岩手大三浦教授,大島助教授に感謝の意を表すると共に,供試品を提供された東芝製薬に謝意を表します。

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Althongh a considerable number of studies on the antihormone against human chorionic gonadotrophin (HCG) administered in the man and laboratory animals such as the rat and rabbit have reported, a little is known about domestic animals.
We performed investigations on the antihomone against HCG (Anti-HCG) in cattle receiving this hormone. Gonadotrophic potencies of HCG used 'in peresent study were assayed biologically and the results obtained are shown in Fig. 1.
In this paper an attempt was made to ascertion the Anti-HCG formation in the sera of a female calf (No. 1 : 3 months of age) and a cow (No. 2 : 8 years of age). The 2 animals were injected intravenously with large quantities of HCG tolerably purified. Doses of HCG injected, intervals of the injection and times of blood collections from them were as shown in figures presented under Tables 2 and 3. Every sample was determined whether it posessed Anti-HCG, by emloying immature female mice, as shown in Table 1.
Results of the determinations of Anti-HCG in the serum samples from the 2 animals were shown in Table 2 and Table 3 respectively. The results obtained were summarized as follows:
1. In cattle No. 1 which was injected with 29, 230 MU of HCG (equivalent nearly to 5 MU per cc. of the blood), the determination of Anti-HCG was negative on the serum collected at 19 days after the HCG injection. Then the animal was received 20 injections of 1, 000 MU of HCG for 39 days. On the 11 th day after the last injection, Anti-HCG was determined as positive, the inhibiting titers of the serum being 6 MU per cc.
2. In cattle No. 2 which was injected with 137, 000 MU of HCG (same blood level as in No. 1), the determination of Anti-HCG was positive on the serum of 17 days after the injection.
3. The serum samples collected from the 2 animals on the 50 th day after the HCG injection revealed inhibitory activities against HCG, the inhibiting titer being about 1, 5-3, 0 MU per cc. Then the determinations were negative on the 100 th day.
4. It was clearly demonstrated that the antihormone was produced in cattle injected with large quantities of HCG.

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In peevious report we observed that the antihormone against HCG was produced in cattle receiving large amounts of this hormone. The dosage of HCG used in the previous experiment, however, was much more than that used generally for the purpose of treating ovarian disorders in cattle. In this paper an attempt was undertaken to ascertain whether the Anti-HCG was produced in cattle which had been injected repeatedly with general dosage of HCG. Serum samples collected from 11 cows with cystic ovaries were used as materials to determine the Anti-HCG. The methods to determine the Anti-HCG in the serum samples were same as described in the previous report. These animals had been injected 13 times with 5, 000 IU-20, 000 MU of HCG for treating ovarian cysts. The treating histories, prognosis and ovarian conditions of these animals and the results of the Anti-HCG determinations are shown in Table 1. The Anti-HCG determinations were also performed on the serum samples collected from 11 control animals which had not been injected with HCG within recent 12 months. The ovarian conditions and the results of the Anti-HCG determinations on the control animals are shown in Table 2.
The results obtained were smumarized as follows:
1. In 6 of 11 cows which had been received 13 injections of 5, 00020, 000 MU or 5, 00010, 000 IU of HCG intramuscularly within recent 4 months in order to treat ovarian cysts, the serum Anti-HCG was determined to be positive. The HCG inhibiting titers of these positive sera were 1.5 MU-24.0 MU or more per cc. The Anti-HCG positive animals did not recover from ovarian cysts in spite of the HCG treatments for 13 times.
On the contrary, in 15 animals in which the serum Anti-HCG was determined to be negative, 2 animals had recovered from ovarian cysts and came in normal estrous cycle and 2 were recovering from ovarian cysts by the HCG treatments. Only one animal was not yet restored by the treatments.
From these results, it is possible that the Anti-HCG in produced in considerable number of cattle with cystic ovaries which were uncurable following repeated treatments with HCG. 2. On all of the serum samples from 11 control animals, including 5 with cystic ovaries, 3 with ovarian dysfunctions and 3 with normal estrous cycles, the Anti-HCG was determined to be negative.
From these results, it is quite possible that the Anti-HCG is not produced in cattle which have not been injected with HCG.

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1. 精溜採取後直ちに注入した第I群では注入後3日から5日までは中間に僅かの低下はあるが100%のdaily fertilityを示し, fertility potentialは注入後12日まで継続した。
2. 24時間保存後注入した第II群では注入後3日目に83.3%のdaily ferilityを示しfertility potentialは9日まで継続した。
3. 48時間保存後注入した第III群のdaily fertilityは略24時間保存の場合と同様であつたがfertility potentialの持続は注入後5日間で,その間受精率は漸次低下しているのが認められた。
4. 第IV群及び第V群即ち72時間及び96時間保存のものでは受精卵は得られなかつた。

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