ラットの唯乳期中妊娠で正常の時期に着床させた後に泌乳量を増させると胚の吸収が起つた。この際黄体ホルモン又は卵胞ホルモンを給与するとこの吸収を防止出来た。維持に必要な卵胞ホルモンの投与期間はplacnetalsignの出現時期までであるが,これでは十分な維持は出来ずpartial abortionが起る。

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An experiment was conducted in which five Corriedale ewes were treated with hormones to produce outseasonal lambs. After the administration of 75 mg of progesterone, 12.5 mg twice-daily or 25 mg daily for three successive days, 1, 000 i. u. of PMS was given on the third day of final progesterone injection. Some of them showed rather weak and irregular oestrus, but all were mated with rams within fortyeight hrs. after PMS treatment. Three out of five thus treated produced four lambs. Case numbers were considered not sufficient to draw a final conclusion, however, the results showed that ewe fertilty during non-breeding season could be expected with Corriedale to a certain extent. Factors which seemed to influence the lambing rate were discussed in the light of the exsisting knowledge of physiology of reproduction in sheep.

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Recently some authors have described that in several kinds of animals the cumulus oophorus is loosened by the enlargement of its intercellular spaces and the oocyte is easily discharged from the mature follicle at the time of ovulation. We have been performing the study of morphological changes in normal bovine reproductive organs, and this report dealt with the morphological observation of the development of ovarian follicles and the examination whether the above mentioned fact is the case in the cow. The ovarian follicles of Japan native cows obtained from the slaughterhouse and Holsteins kept in our laboratory were used. Serial sections of the cumulus were made and stained with hematoxylin-eosin. Results and discussions are as follows:
(1) The relative growth curve between the major axis of the oocyte and that of the follicle (Ma in chart 1) was almost the same with that obtained in white mouse by BRAMBELL. This curve was divided into two phases; at the end of the first phase the ooecyte gained about 3 times of the major axis, and then about 3.6 times at the end of the second phase compared with that in the primary follicle. The relative growth curve between the minor axis of the oocyte and the major axis of the follicle (Mi in chart 1) showed the descendance in the last stage of the second phase. It is, however, very difficult to decide the cause of the result; it might be because of fixation, a few cases, or the reduction of oocyte size by the first maturation division.
(2) The antrum began to appear in follicle of 437 × 294 microns in the major and minor axes.
(3) The zona pellucida began to appear on the surface of the oocyte with the average size of 82.4 × 63.2 microns in the beginning of the second phase. The thickness of the zona pellucida which was 1.0 × 3.51.5 × 3.5 microns did not markedly increase or decrease in spite of the growth of the oocyte.
(4) The cell, so-called “stellate cell”, which has the delicate processes and connects each other was seen in the follicular epithelium when the follicle grew larger than the secondary follicle. These stellate cells were localized in the region of the cumulus and became indistinct in the other region following the formation of the cumulus oophorus.
(5) Although the cumulus of the follicle of 15mm. in diameter situated near the rupture point of the follicle, in the follicle of 611 mm. in diameter it located on the opposite site. These facts may be suggesting the movement of the cumulus. In the mature follicle of 1217 mm. in diameter, however, the cumulus situated on various positions of the follicular wall.
(6) In the follicle more than 1mm. in diameter several small processes of the theca interna were found more distinctly in the region of the cumulus, but we could not find the penetration of the capillaries into the membrana granulosa.
(7) In the follicle with the numerous corpuscles which are deeply stained with hematoxylin, the membrana granulosa was very thin because of the disappearance of its cells and the small processes of the theca interna were not clear in the region other than the cumulus. The oocyte and the cells of the cumulus, however, did not show the degenerative finding.
(8) Follicular epithelial cells adjacent to the theca interna varied their forms according to the growth of follicle. They were flattened or cubical in the primary follicles, columnar when the antrum appeared, and thereafter cubical again in the follicles more than 6mm. in diameter.
(9) The corona radiata was found to have several cell cords which run radially and connect it to the surrounding and among these cords several spaces were always seen after the formation of the cumulus. The space itself, however, was found to originate from the ones already present in the secondary follicle.

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Morgantini's pregnacy diagnosis test, the colour reaction of blood plasma produced by heating in the presence of cupric carbonate, was performed with bovine blood plasma in various conditions. But the availability of the test resulted in definitely negative since results of the reaction happened to be almost all positive regardless to the pregnancy or not.
Next, spermine, which regarded as the reactive substance in the preceding colour reaction, was detected together with other amines in blood plasma by paper-chromatography to know whether the amines appeared specifically in pregnant plasma. Volatile amine fraction was prepared from the TCA soluble fraction by the vapour distillation with Kjeldahle apparatus. The findings by the paper-chromatography showed that two spots, corresponded to spermine or spermidine and putrescine, also existed independent of the animal condition either pregnancy or lactation. Therefore, the authors completely failed to ascertain the Morgantini's investigation as the pregnancy diagnosis test based on either the colour reaction with cupric carbonate or the detection of spermine with paper-chromatography in bovine plasma. The occurrence of polyamines in the bovine blood plasma and the pregnancy tests which seemed to be related to this test was briefly discussed.

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1) C.Mの塗抹乾燥標本に晶出した結晶像は水分に対して不安定で破壊され易く又乾燥法或は塗抹の厚薄によつてその像に差異を生じ易いが,吾々の所謂C.G法はこれらの外的感作に影響されることなく極めて安定しており,操作手技は簡単にして臨床的に価値あることを認めた。
2)粘液像を直後像及び乾燥像に区分し,それぞれの出現像の形状により9型に分類して観察した結果,性周期の判定が可能である。特に直後及び乾燥像の両者を併用組合せることにより一層顕著となることを認めた。即ち発情期では直後像はI型が,乾燥像はIII単形,V型が高い出現率を示した。
黄体期には直後及び乾燥像共にIII棘形,皿桿形及びIV型の出現が目立ち,両期の移行期には直後像ではIII枝形が,乾燥像ではII型,III長形,III羽形の出現が多くなる傾向を認めた。
3)妊娠期においては直後及び乾燥像共にIII棘形,III桿形及びIV型が特異的に出現し,その他の型像は殆んど認めなかつた。この点妊娠診断の補助として価値あることを認めた。
4)子宮内膜炎を有する例においては健康例に比して,発情期では直後像にIII枝形が,乾燥像にIII網形,III樹形が多くなり,黄体期では直後像,乾燥像共にIII桿形の出現が少く更に乾燥像にIV型の出現は全例に認めなかつた。
以上の如く健康例に比し移行期像が多く出現し,周期の特異型像が認め難くなることは組織学的に分泌腺の機能に異常を認められること¹⁰⁾,又結晶形成能の低下,内分泌機能の失調^3,11)及びC.Mの化学的組成の変化¹¹⁾等の事実から,更にC.Mの性状特に粘稠度の変化,架片量及び細胞成分等⁶⁾に影響されるものと考えるが,尚今後の研究に俟ちたい。

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The minimal effective dose of a preparation of porcine FSH was determined by the assay based on the resumption of ovarian follicular development and the increase of ovarian weight in hypophysectomized immature rats. This preparation contained about 23% of ICSH by the ventral prostate assay in hypophysectomized rats.
At doses of 50100γ the minimal stimulation of follicles was detected microscopically, and the follicular growth was evident at a dose of 200γ. In this dose, the definite increase of ovarian weight was, also, observed.
From these results, total dose of 200γ of the preparation of FSH was determined to be the minimal effective dose stimulating both the follicular development and the ovarian weight increase.

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