妊娠前半期にて下垂体を別出されたラットにおいては悉く,妊娠の中断がみられる。
Sprague-Dawleyラットでは,妊娠10日目(腟垢に精子が検出された日を妊娠0日)にて下垂体を別出すると,91%がそのまま妊娠を維持するが,妊娠9日目に下垂体を別出すると,87%に妊娠の中断がみられる(妊娠21日目にて解剖)。
妊娠前半期の間に下垂体を別出し,HCGを術時から妊娠前半期の間だけ(妊娠9日目まで)1日1回投与し,妊娠維持を検討してみると,下垂体別出が妊娠4日,5日目に行なわれたラットに対してはHCG日量40IU,妊娠7日目以後に行なわれたものでは日量4IU投与で高率に妊娠を維持させることがわかった。
一方,HCGがprogestin分泌と共にestrogen分泌作用をも有することが知られているので,前述のHCGと同じ方法で,下垂体別出ラットに対してestroneの単独投与を試みた。その結果,妊娠7日目以後に下垂体を別除したラットにestroneが妊娠を維持させ得ることが明らかとなった(妊娠7日目下垂体別出群,estrone日量1.0~4.0μg投与で,妊娠維持率は44~50%;妊娠9日目下垂体易出群,estronel.0~2.0μg投与で,妊娠維持率29~71%;妊娠9日目下垂体別出群,estroneO.5~1.0μgで,妊娠維持率17~88%)。したがって,妊娠前半期の末期に近い妊娠日数にて下垂体を別出された群では,HCGによる妊娠維持作用を,すべて,HCGのprogestin分泌作用によるものとみなすことはできないように思われた。

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Quantitative test for the determination of individual PMSG in the pregnant mare is one of the important tools for pregnancy diagnosis. The present paper reports on the quantitative assay of standard PMSG and pregnant mare sera using antibody agar plate method. The research using antibody agar plate were performed according to modification of Fafey's technique³⁾. With this technique, specific anti-PMSG immuno rabbit serum (IgG) is mixed uniformly in an agar plate. Antigen containing solutions are placed in small antigen wells cut in the agar. A concentric ring of antigen-antibody reaction forms around the antigen well. By graphically comparing the ring diameters with those of appropriate standard, the concentration of the test sera of pregnant mares can be determined. The mean PMSG levels in 30 pregnant mare sera were found to be minimum 60 IU/ml and maximum 210 IU/ml from pregnant mare sera. However, for definite evalution of the samples, more experimental data will be needed. In this study, by means of the antibody agar plate technique, it was possible to use the anti-PMSG serum for an immunoassay for qua, titative analysis.

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The Serotest, immunological pregnancy tests of mares, was studied on 282 sera of 196 mares and 3 stallions at Tokachi and Hidaka districts in Hokkaido, from May to September in 1967.
Results obtained may be summarized as follows;
1. Accuracy of tests on nonpregnant mares and stallions was 100.0 %, but on pregnant mares, it varied with gestation periods. That is, at 1 to 29, 30 to 44, 45 to 80, 81 to 120 and more than 121 days after the last service, accuracy of tests was 6.3, 51.7, 96.6, 40.0 and 40.0 % respectively.
2. Positive reactions with this test were first observed at 3946 days and continued to 76126 days of gestation through long-term observations of 5 pregnant mares.
3. Accuracy of tests on light horses was seemed to be higher than that of half blood or heavy horses.
4. Eight mares out of 160 were suspected to have aborted through this tests and biological assays.

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Nobunaga et al. observed that the interval between the ovulation induced by HCG injection at 4 PM on the day of diestrous stage and the first spontaneous ovulation occurring thereafter was 72 hours.
This report deals with the interval between the ovulation induced by a single intravenous injection of HCG (injection was done at 0 AM, 9 AM, or 4 PM on a day during stage IV, V, or I respectively) and the first spontaneous ovulation taking place thereafter.
1) In a few of the animals injected with HCG at 0 AM on the day during stages IV and V, first spontaneous ovulation after the ovulation induced by HCG took place 76 hours after the HCG injection (or 64 hours after the induced ovulation).
In the most of them, however, those ovulations occurred 100 hours after the HCG injection (or 88 hours after the induced ovulation).
In animals injected with HCG at 0 AM on the day of stage I, the time interval between the injection and first spontaneous ovulation after the induced ovulation was 100 hours (or 88 hours after the induced ovulation).
2) In a few of the animals injected with HCG at 9 AM on the days during stages IV and V, first spontaneous ovulation were observed to have possibly taken place 67 hours after the HCG injection (or 55 hours after the induced ovulation).
In the most of them, however, those ovulations were seen 91 hours after the HCG injection (or 79 hours after the induced ovulation).
In animals injected with HCG at 9 AM on the day of stage I, those ovulations was occurred about 115 hours after the HCG injection (or 103 hours after the induced ovulation).
This time interval is longer than 91 hours when was injected at 9 AM on the day during stages IV and V.
3) In animals injected with HCG at 4 PM on the day during stages IV and V, first spontaneous ovulations were discoverd about 84 hours after injection (or 72 hours after the induced ovulations), without exception.
In animals injected with HCG at 4 PM on the day of stage I, first spontaneous ovulations took place 108 hours after the HCG ihjection (or 96 hours after the induced ovulation).
Namely, the time interval between the ovulation induced by HCG injection at 4 PM on the day of stage I and the first spontaneous ovulation thereafter was 96 hours, when was longer than 72 hours, or the time interval between the ovulation induced by HCG injection at 4 PM on the day during stages IV and V, and the next spontaneous ovulation.
4) Accordingly, after the ovulation induced by HCG injection some time between 0 AM and 4 PM on the day of stage IV, first spontaneous ovulations were discoverd on the morning of the same day in the most of the animals injected.
After HCG injection performed some time between 0 AM and 4 PM on the day of stage V, first spontaneous ovulations were observed on the morning of the same day.
In a few of the animals injected with HCG at 0 AM on the day of stage V, next spontaneous ovulation took place on the morning of the same day one's due to the cases of HCG injection on the day of stage IV.
In the case of HCG injection at 0 AM on the day of stage I, next spontaneous ovulation occurred on the next morning of the day one's due to the case of HCG injection at 4 PM on the day stage V.
In the case of HCG injection at 9 AM and 4 PM on the day of stage I, next spontaneous ovulations were delayed and took place on the next morning of the day of first spontaneous ovulation when HCG was injected at 0 AM in stage V.
5) The fiirst spontaneous ovulation after the induced ovulation was observed over a period from 0 to 4 AM in all the cases.
6) In all the cases of first spontaneous ovulation after the induced ovulation, an ovulation-blokade by pentobarbital (Nembutal) anesthesia was seen when injected with Nembutal intraperitoneally (30 mg/kg) at 0 PM on the day before the first spontaneous ovulation.
It was not seen however, when injected with Nembutal at 4 PM on the day before the ovulation.

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家兎を用いてHCGに対する抗血清を作製し,そのAnti-HCGのPMSの生物学的作用に対する効果を調べた結果以下のことが判明した。
1.Anti-HCGはPMSの有する排卵誘起能を抑制する。これに必要なAnti-HCG量はHCGの排卵誘起能を抑制する量の約8~16倍量を必要とした。
2. Anti-HCGはPMSの有する卵胞発育作用をも抑制する。これに必要なAnti-HCGの量はPMSの排卵誘起能を抑制する量の約4倍を必要とする(HCGの排卵誘起力抑制量の32~64倍量)。
3.Anti-HCGは卵胞ホルモン分泌能をも抑制するが,これはPMSの卵胞発育作用を抑制する結果と思われる。

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