牛の発情期頸管粘液におけるSR及びCDSの発現と粘液組成との関係について,粘液中の固形分,NaCl及びMucinclotの含量並びにそれらの含量比の点から検討を加え,次の知見を得た。
1.CDSが高度の発現を呈するにはNaClが固形分の凡そ過半を占めれぽ足りるが,SRの発現も同時に高度であるにはさらにNaC1は固形分の約57%以上を占め,且つNaC1濃度が0.9%以下という組成関係が必要である。
2.Mucinclot含量0.5%以下において両現象は共に高度の発現を呈するが,この場合もNaCl濃度が0.9%を超すとCDSの発現は高度でSRは低調という例を生ずる。
3.NaC1及び(又は)Mucinclot含量をin vitroにおいて増減し,その含量を上記の域から上下させることによりSRを高低させることができる。
4.以上のことがらから,SRおよびCDSの両現象の発現は粘液組成殊にNaC1及びMucinclotの含量並びに両成分の含量比,NaClが固形分中に占める割合によつて大きく左右されるものであることを明かにした。
なお,本報の要旨は第50回日本獣医学会において発表した。

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Many workers have studied on the induction of superovulation in the cow by application of gonadotropin. However, full information on controlling intervals between gonadotropin and induction of superovulation is still lacking. The main purpose of this study is to induce superovulation in the cow within certain limited period after gonadotorpin administration.
1. Induction of superovulation in the cow.
First gonoadotropin injection: Twelve cows in various stages of the estrous cycle were injected subcutaneously with 2, 500-3, 000 I. U. of PMS alone or 2, 000 I. U. of PMS combined with 2, 000 I. U. of HCG in order to stimulate follicular development. Second gonadotropin injection : The 1st injection was followed by intravenous injection of 1, 500-2, 000 I. U. of HCG in order to accelerate ovulation, 3 to 5 days after the ist injection when several developing follicles were palpated per rectum in all of the animals. Ovarian reactions in the treated animals were examined by rectal palpation every day :after the gonadotropin injections. Three of the treated animals were slaughtered 7-11 days after the 1st injection, for the purpose of examining ovarian reactions in detail. Results obtained are shown in Table 1. It was observed by rectal palpations that the great majority of developed follicles were in-duced to ovulation within 24-48 hours after the 2nd gonadotropin injection and few successive ovulations were followed within the next 24 hours. Multiple formations of corpora lutea were observed later. Eventually, 4-29 ovulations were induced in all of the treated animals. The ovarian reactions of cow No. 1 is shown schematically in Fig. 1. On the course of the experiments, ovum recovering tests by means of oviduct flushing were performed with success in 5 of 6 animals.
2. Recurrence of the estrous cycle in the treated animals
Further observations, regarding ovarian conditions and recurrence of estrous cycle after the superovulation inducing experiments, were continued for a long time in 7 of the treated animals. The results obtained were shown in Table 2. Multiple corpora lutea derived from superovulation began to regress about 18-46 days after begining of the experiment. And then, normal estrus recurred in all of them. Following estrous cycles were as normal as those before the experiment.
3. Ovarian reactions to repeat administrations of gonadotropin
In these experiments, cow No. 3-1 is the same animals as No. 3-2, and interval between the two.experiments is 128 days. Cow No. 1 was employed twice in the same kind of experiment, 50 days and 172 days after the 1st experiment respectively. Although superovulations were induced in the repeated experiments in cow No. 3, ovaries of cow No. 1 failed to ovulate in the repeated administrations of gonadotropin. It is possible that the ovarian refractoriness in the latter animal is due to ontihormone.

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ラットの子宮に局所的に卵巣ホルモンを作用させる新らしい方法を発見した。これは子宮広靱帯に附着している脂肪組織内にホルモン油溶液を注入するもので,比較的自然に,子宮には全く傷害を与えずに局所的にホルモン作用ン作用を及ぼし得るものである。この方法を用いてprogesteroneの着床惹起作用を哺乳期中の妊娠ラットで吟味したところ,20μg以上で殆んで全例局所的な着床を起し得た。この作用の及ぼされる範囲は,progesterone投与部位に接した血管分枝の分布域内に限られ,用量を100μgまで上げても変らなかつた。以上の結果から泌乳中には卵の着床を起すに足る量の黄体ホルモンが子宮に供給されていないと考えられる。

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Effects of HCG on the reproductive system of hypophysectomized immature male rats were compared with that of ICSH by routine assay method for ICSH8).
Twenty three day old male rats of the Wistar strain, weighing 28 to 40 g, were hypophysectomized. Two pays after the operation, injections of hormone were initiated and continued for 4 days. Autopsy was performed on the next day of the last injection. Crude (50 M. U. /mg) and purified (1000 M. U./mg) preparations of HCG were used.
HCG stimulated effectively the weight of the ventral prostate glands. One prostate unit (the amount of gonadotrophic substances which produce 100% increase of weight of the ventral prostate in hypophysectomized immature rats above that of the hypophysectomized controls) of HCG was about 0.1 and 0.2 mouse unit of crude and purified samples respectively. By larger doses, HCG stimulated the ventral prostate more effectively than ICSH.
A specific response of the weight of the seminal vesicles stimulated by HCG was obtained. The rapid rise of the response curve of weight of the seminal vesicles occured after the rise of the curve of the ventral prostate. It reached the maximum at 10 or 50 Pr. U. (1 or 10 M. U.) in both preparations of HCG respectively. and fell rapidly even though increasing dose levels of hormone were given.
HCG also stimulated more effectively the weight of epididymides than ICSH.
Although, the testicular weight was increased and a convex curve was obtained by the crude preparation of HCG, only slightly weight stimulated by the purified preparation. The result is distinct from those given by Simpson et al. They administered HCG for 15 days to hypophysectomized 40 to 43 day old rats and obtained the marked increase of weight of testes and accessory organs. In this experiment, the accessory organs showed marked development, however, only slight increase of testicular weight was observed by administration of high levels of HCG.

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