To evaluate the freezing resistance of tea shoots during winter and early spring, the electrolyte leakage from tea shoots after cold treatment was assessed by using a conductivity meter. Increase in electric conductivity reflected tissue injury immediately after cold treatment. The variation in electric conductivity indicated the differences in cold resistance among cultivars (‘Sofu’, ‘Yabukita’, and ‘Fushun’), in addition to the changes in cold resistance from winter to early spring and differences in cold resistance based on whether or not medium pruning treatments were performed.
The biosynthetic pathways of flavonoids and the enzymes involved in those pathways are shown in Figures 1, 2, 3, and 4, and Table 1. The flavonoids in the metabolic maps include flavones, flavanones, dihydroflavonols, leucoanthocyanidins, flavonols, anthocyanidins and flavanols (catechins).