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Volume 45, Issue 10
Displaying 1-7 of 7 articles from this issue
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Function and morphogenesis of transplanted cellsSawako KUWAHARA, Ken-ichiro HATA, Hideaki KAGAMI, Yukio SUMI, Hirokazu ...1999 Volume 45 Issue 10 Pages 603-608
Published: October 20, 1999
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSWe have described culture methods for normal salivary gland cells, using 3 T 3 cells as a feeder layer. These cultured salivary gland cells are morphologically and biologically similar to normal salivary gland cells. However, the properties of cells cultured in vivo are unknown. In this paper, the second passage of cultured salivary gland cells was applied to atelocollagen sponges, which were then transplanted to the back of nude mice.
The mice with transplanted cells showed liquid accumulation on the back, whereas the control animals did not.
The liquid was viscous, faint brown in color, and contained amylase (mean, 2175 IU/l).
Immunohistochemical staining showed that approximately 50 percent of transplanted cells were amylase positive 24 to 48 hours after transplantation. Four weeks after transplantation, the atelo collagen sponge was completely absorbed, and epithelial cells were distributed in a band-like fashion. The transplanted cells did not show ductal or acinar formation. Our results show that salivary gland cells cultured by our method retain the characteristics of salivary gland cells in vivo.
It may be necessary to establish a novel in vivo model of salivary glands by using key growth factors.View full abstractDownload PDF (8353K) -
A comparison between pectoris major myocutaneous flaps and forearm flapsTakahiko SHIBAHARA, Hiroyasu NOMA, Yoshito TAKASAKI, Kiyohiro KASAHARA ...1999 Volume 45 Issue 10 Pages 609-614
Published: October 20, 1999
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSWe investigated the degree of recovery of sensory functions by pectoralis major myocutaneous flaps (muscle flap group) and forearm flaps (skin flap group). Twenty-six patients who underwent ablative surgery were studied. A pectoris major muscle flap was used in 8 patients, and a forearm flap was used in 18 patients. SW sensory, two-point discrimination (2 PD), thermal pain, and microcirculatory blood flow were measured. The SW value was maintained at a high level in the muscle flap group, but decreased gradually after 60 months in the skin flap group. The time course of 2 PD indicated a faster recovery in the skin flap group. Thermal pain values did not differ significantly between the two groups.
Blood flow in the oral cavity mucosa was higher than that in the skin. In most patients, blood flow in the skin flap was similar to that in the skin. There were no marked timerelated changes in blood flow in either group, but blood flow in the forearm flap was clearly lower.View full abstractDownload PDF (800K) -
Takahiko SHIBAHARA, Hiroyasu NOMA, Takeshi NOMURA, Isamu KAMIYAMA, Eiz ...1999 Volume 45 Issue 10 Pages 615-619
Published: October 20, 1999
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSFree skin grafts and skin flaps are now widely used for cranio-maxillofacial reconstruction. As for shaping, local skin flaps from donor sites adjacent to the reconstruction area are advantageous in terms of color match and texture match. However, there are the problems with respect to volume limitations and scar formation in adjacent areas of the face, and skin grafts or distant skin flap grafts are often used to facilitate the design of skin flaps. If the tissue expansion principle is used to enlarge volume, these problems are also solved. We used tissue expansion, still only rarely used in the oral and maxillofacila region, to treat cheek scar atrophy, fistula, and microsomia. We report these cases because favorable results were obtained.View full abstractDownload PDF (8519K) -
Hideaki KITADA, Shoji HARADA, Makoto ISHIKAWA, Ken-ichi NOTANI, Hirosh ...1999 Volume 45 Issue 10 Pages 620-622
Published: October 20, 1999
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSA case of metastasis to the lateral lingual lymph nodes in a patient with tongue cancer is reported. The patient wase a 73-year-old man with a moderately differentiated squamous cell carcinoma of the left lingual margin (T 3 N 2 bM O). Metastasis to the lateral lingual lymph nodes was detected by computed tomography and magnetic resonance imaging preoperatively. After external radiation, bilateral neck dissection and hemiglossectomy were performed by a pull-through operation. Histopathological examination revealed that the lateral lingual lymph nodes had been extensively degenerated by the radiation.View full abstractDownload PDF (2787K) -
Akihiro MIYAZAKI, Takashi IDE, Makoto NOGUCHI, Tatsuru SUYAMA, Itaru N ...1999 Volume 45 Issue 10 Pages 623-625
Published: October 20, 1999
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSA case of plasmacytoma in the left maxillary alveolar process with buccal lymph node involvement is reported. The patient was a 74-year-old man who had swelling of the left side of the maxillary gingiva. An incisional biopsy was performed, resulting in a diagnosis of plasmacytoma. There were no abnormal findings in serum protein, urinary protein, or bone at other regions. The patient underwent partial maxillectomy with subsequent radiotherapy. The present case was diagnosed to be IgA-λ type plasmacytoma by means of immunohisto-chemical study. There has been no evidence of local recurrence, lymph node metastasis, or transition to multiple myeloma as of 1 year 10 months after surgery. However, long-term follow up is required.View full abstractDownload PDF (3318K) -
Akira ENDO, Hiroshi FUKUDA, Kenji HASHIMOTO1999 Volume 45 Issue 10 Pages 626-628
Published: October 20, 1999
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSWe report a case of dialysis amyloidosis associated with masses in both lateral edges of tongue. Symptoms improved after resection of the mass of the tongue. A 55-year-old woman was referred to our department on December 5, 1995. She had swelling and pain of right and left margins of the tongue. She had received dialysis treatment since 1970 for chronic renal failure. We suspected that the masses of tongue might be amyloid deposition and removed the left mass surgically to alleviate dysphemia and dysfunction of the mastication. To diagnose the mass, we carried out Congored staining, hematoxylin and eosin staining, and sensitivity tests for KMnO4 treatment. We recognized ameyloid deposition and macrophage invasion around the small vascular wall and transverse muscle of the tongue. The type of amiloidosis was not AA-type.
The results suggest that masses in both margins of the tongue are one of the symptoms of dialysis amyloidosis and that resection of these masses is one of the efficient methods to improve symptoms.View full abstractDownload PDF (6000K) -
Chie YANAI, Ken OMURA, Tomomi YAMASHITA1999 Volume 45 Issue 10 Pages 629-631
Published: October 20, 1999
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSA 34-year-old woman with nodular fasciitis arising in the right masseter muscle is reported.
The patient had an indolent, 2-cm mass in the right buccal region. The mass had been present for 2 months. Ultrasonography, MRI, and cytological examination revealed a benign tumor arising in the masseter muscle.
She underwent peroral excision of the mass. Histopathologic examination of the surgical specimen revealed a well encapsulated mass composed of proliferations of immature fibroblasts within a myxomatous matrix, accompanied by proliferations of capillaries and infiltration of inflammatory cells. However, fibroblasts showed neither polymorphism nor atypia. From these findings, the mass was diagnosed as nodular fasciitis.
The postoperative course was uneventful, and 1 year 9 months postoperatively the patient was well, with no signs of recurrence.View full abstractDownload PDF (3330K)
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