All issues
Volume 46, Issue 9
Displaying 1-9 of 9 articles from this issue
- |<
- <
- 1
- >
- >|
-
Toru KUWAHARA, Koichi SHIBASAKI, Akira YAMAGUCHI2000 Volume 46 Issue 9 Pages 497-502
Published: September 20, 2000
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSWe performed molecular typing of Helicobactor pylori (H. pylori) in saliva and gastric mucosa by a PCR-RFLP method. The results were as follows.
1. H. pylori DNA was detected in 25 (78.1%) of 32 cases in saliva and in all 32 cases in cultures of gastric mucosa.
2. Molecular typing of H. pylori with the use of restriction enzyme Hae M showed 6 types (A-F).
3. In many cases, molecular typing of H. pylori was type A [16 (64.0%) in saliva and 18 (56.3%) in gastric mucosa]. Type B was found in saliva in 5 cases (20.0%) and in gastric mucosa in 7 (15.6%). However, there was no significant difference in molecular typing of H. pylori between saliva and gastric mucosa.
4. In saliva and gastric mucosa, molecular typing of H. pylori was similar in 23 of 25 cases (92.0%)[AA, 15 (69.0%), B-B, 5 (20.0%)].
5. As for agreement of molecular typing, type A was consistently found in many cases of gastric ulcer, duodenal ulcer, and gastroduodenal ulcer. However, there was no significant diference between these diseases.View full abstractDownload PDF (3631K) -
Kazunari OOBU, Yukihiro MAEDA, Hiromasa YOSHIKAWA, Satoru OZEKI, Masam ...2000 Volume 46 Issue 9 Pages 503-510
Published: September 20, 2000
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSThe effect of hyperthermo-radio-chemotherapy (R+C+H) given as preoperative treatment for carcinomas of the lower gum was examined.
Hyperthermia was combined with conventional radio-chemotherapy (R+C) consisting of 22.5Gy of radiation and 110mg of bleomycin. Eighteen patients treated by R+C+H and 18 patients treated by R+C were studied. The mandible, resected at operation, was examined histopathologically.
Clinically, 9 patients had a complete response (CR), and 7 had a partial response (PR) in the R+C group. In the R+C+H group, 10 had a CR, and 6 had a PR. The histopathological response to treatment was evaluated according to Shimosato's classification in both soft tissue and bone invaded by tumor in the resected mandible. Eight patients (44.4%) in the R+C group and 7 (38.9%) in the R+C+H group showed Grade DI or IV in soft tissue. Three patients (21.4%) in the R+C group and 9 (69.2%) in the R+C+H group showed Grade IQ or IV in the invaded region. The histological response rate (Grade DI+IV) in the R+C+H group was significantly higher than that in the R+C group (p<0.05). Furthermore, in patients in the R+C+H group who had a CR clinically, tumor cells almost completely disappeared from the region of the mandible invaded by tumor.
These results suggest that R+C+H therapy is an effective treatment for carcinomas of the lower gum that involve the mandible.View full abstractDownload PDF (4530K) -
Part 3: Oxidative stress and the induction of genomic instability by long-term EGF treatmentDaichi NAKATA, Toshiyuki SHIBATA, Hiroki NAGAYASU, Takashi KAWANO, Hir ...2000 Volume 46 Issue 9 Pages 511-518
Published: September 20, 2000
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSWe have previously reported that long-term epidermal growth factor (EGF) treatment enhanced the malignant phenotype of regressor ER-1 cells and might affect the genomic alteration of ER-1 cells. In this study, we examined whether EGF stimulation might induce reactive oxygen species (ROS) in ER-1 cells and whether ROS could affect genomic instability. ROS of ER-1 cells induced by EGF stimulation for 1 month was observed with a confocal laser-scan microscope after 2', 7'-dichlorofluorescein diacetate (DCFH-DA) staining. ROS of ER-1 cells was significantly augmented by EGF stimulation, and ROS spread over the nuclei after stimulation. To study the effect of ROS on ER-1 cells, we measured the amount of glutathione peroxidase (Gpx), radical scavenger enzyme, and 8-hydroxydeoxyguanosine (8-OHdG), which is a mutation indicator. The amount of Gpx was reduced by EGF stimulation and recovered after sodium selenite treatment. Furthermore, to study modulation of the malignant phenotype of ER-1 cells, each treated ER-1 cell was intrapenitorially transplanted into syngenic SHR-rats. Tumorigenicity of ER-1 cells was significantly enhanced by EGF, and selenite treatment completely inhibited the augmentation of tumorigenicity caused by EGF stimulation. This observation was consistent with the in vitro results. Then, to examine the effects of ROS induced by EGF stimulation on the genomic instability of ER-1 cells, DNA fingerprinting assay was done. In this assay, abnormal bands were observed in four clones (4/19 clones) after EGF stimulation. These results suggest that long-term EGF stimulation can affect the genomic alteration of ER-1 cells and that this phenomenon is possibly caused by EGF inducing ROS.View full abstractDownload PDF (9752K) -
Hajime FUJITA, Kazuhiro ONO, Masaki NAGATA, Akihiko IIDA, Nobuyuki IMA ...2000 Volume 46 Issue 9 Pages 519-526
Published: September 20, 2000
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSWe retrospectively investigated the clinical findings and treatment of cleft lip, cleft palate, or both associated with chromosomal karyotype aberration. The patients were examined at the Second Department of Oral and Maxillofacial Surgery, Niigata University Dental Hospital between April 1982 and March 1998.
The findings obtained were as follows:
1. Twelve of 806 patients with cleft lip, cleft palate, or both (1.5%) had chromosomal aberrations. Four of these patients had bilateral cleft lip, alveolus, and palate, and 8 had cleft palate. In 10 primary cases, the incidence of chromosomal aberrations was 4.4%(3/68) in patients with bilateral cleft lip, alveolus, and palate and 5.1%(7/138) in patients with cleft palate.
2. Various types of chromosomal aberrations (No.13, 15, 21 numerical abnormalities and No.1, 2, 4, 5, 6, 7, 9, 13, 18, 21 structural abnormalities) were found, but there was no evidence of an association between the type of chromosomal aberration and the type of cleft lip or palate.
3. A history of spontaneous abortion was confirmed in the mothers of five patients, and similar chromosomal aberrations in blood relatives were confirmed in two patients.
4. Eight patients had major anomalies, and all 12 had minor anomalies. Mental retardation was confirmed in all patients.
5. Among 10 patients with primary cleft lip, cleft palate, or both, 1 underwent cheiloplasty and 5 underwent palatoplasty under general anesthesia. After the physical condition of the patient had stabilized, cheiloplasty was carried out at about 1 year of age and palatoplasty at 2 to 3 years of age in close cooperation with pediatricians and anesthesiologists. To minimize trauma, surgery should be done in one step.View full abstractDownload PDF (962K) -
Hiroki BUKAWA, Shinjiro AOKI, Nobuya FURUKAWA, Hiroshi CHIKUMARU, Ryoi ...2000 Volume 46 Issue 9 Pages 527-529
Published: September 20, 2000
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSA 49-year-old woman was admitted for treatment of gingival carcinoma of the mandible. Tumor resection of the right side of the mandible, partial resection of the right side of the maxilla, right functional neck dissection, and reconstruction using a left fibulo-cutaneous free flap were performed. Infections due to methicillin-resistant strains of Staphylococcus aureus (MRSA) developed 7 days later. Administration of vancomycin (VCM) was effective in controlling the infections, but caused neutropenia. Fourteen days after operation, neutrophils became undetectable. Twenty-four-hour continuous intravenous infusion of granulocyte colony stimulating factor (G-CSF) was effective against agranulocytosis. The stimulation index of VCM on drug lymphocyte stimulating tests (DLST) was 713 (normal<180), demonstrating that VCM had caused the agranulocytosis.View full abstractDownload PDF (3667K) -
Yasutaka OJIMA, Masahiro UMEDA, Hideki KOMATSUBARA, Naruki NISHIMATSU, ...2000 Volume 46 Issue 9 Pages 530-532
Published: September 20, 2000
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSA case of leiomyosarcoma of the maxillary sinus is presented. A 61-year-old man was referred to our hospital because of swelling of the left cheek. Clinical and radiographic examination revealed a tumor of the left maxillary sinus. The tumor invaded into the surrounding tissue. He underwent left total maxillectomy followed by postoperative radiotherapy and chemotherapy with THP-adriamycin, vincristine, and cyclophosphamide. Histologic examination revealed atypical, spindle tumor cells with a positive reaction for a-smooth muscle actin immunohistochemically. A diagnosis of leiomyosarcoma was made. He is free from disease 61 months after the operation.View full abstractDownload PDF (6654K) -
Maki FUJIKAWA, Hiroyasu NOMA, Yoshito TAKASAKI, Takashi TAKAKI, Takash ...2000 Volume 46 Issue 9 Pages 533-535
Published: September 20, 2000
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSTo study the effectiveness of vibration esthesiometry for evaluating neurosensory disturbance (NSD), vibratory sensibility and pressure sensibility threshold tests were performed bilaterally in 31 patients before and 1, 4, and 8 weeks after sagittal splitting ramus osteotomy. An SMV-5 vibration esthesiometer and an SW-tester were used for the tests.
1, 4, and 8 weeks after osteotomy, the incidence of NSD as detected by vibratory sensibility tests was 66.1%, 59.6%, and 62.9%, respectively, while the corresponding values on pressure sensibility threshold tests were 56.4%, 29.0%, and 27.0%, respectively.
The SMV-5 vibration esthesiometer is useful in detecting NSD in the maxillofacial region, because it was more sensitive than the conventional pressure sensibility threshold test using an SW-tester.View full abstractDownload PDF (3235K) -
Tomoe CHUUJOH, Kenji IZUMI, Susumu SHINGAKI, Tamio NAKAJIMA, Takafumi ...2000 Volume 46 Issue 9 Pages 536-538
Published: September 20, 2000
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSA case of ranula in a neonate is reported. A 2-month-old infant was found to have a small swelling in the floor of the mouth at birth. Initial examination revealed a fluctuant, translucent swelling with protrusion from the mouth. The lesion arose in the left side of the mouth floor. A computed tomographic examination confirmed the presence of a cystic lesion, un associated with the sublingual gland. The clinical diagnosis was a sublingual ranula. Although marsupialization was performed three times, the swelling recurred and returned to its original size soon after treatment. Three months later, extirpation of the cyst and the sublingual gland was performed. The postoperative course was uncomplicated, with no signs of recurrence for 6 months. The histological diagnosis was mucous retension cyst. To prevent recurrence, extirpation of the sublingual gland was effective, even in an infant.View full abstractDownload PDF (3770K) -
Hiromitsu KOEDA, Yoshiko MOTOHASHI, Kenichiro SUGA, Hitoshi OHATA, Chi ...2000 Volume 46 Issue 9 Pages 539-541
Published: September 20, 2000
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSWe report a case of orbital cellulitis arising from odontogenic sinusitis in a 31-year-old woman with unilateral exophthalmus, diplopia, pain, and swelling extending to the orbit from the buccal region. The patient was admitted to our hospital. She was unable to open the left eye and had diplopia. Hypoesthesia was present in the left buccal region. In this patient, orbital cellulitis was arose from ethmoidal sinusitis via odontogenic sinusitis. We operated to open the sinus and drain the orbit. Surgical treatment was necessary in this patient because orbital cellulitis can produce serious complications, such as optic neuritis, optic atrophy leading to loss of vision, cavernous sinus thombosis, orbital apex syndrome, meningitis, brain abscess, and death.View full abstractDownload PDF (6317K)
- |<
- <
- 1
- >
- >|