Japanese Journal of Oral and Maxillofacial Surgery
Online ISSN : 2186-1579
Print ISSN : 0021-5163
ISSN-L : 0021-5163
Volume 45, Issue 2
Displaying 1-11 of 11 articles from this issue
  • Naoko MURATA
    1999 Volume 45 Issue 2 Pages 65-72
    Published: February 20, 1999
    Released on J-STAGE: July 25, 2011
    JOURNAL FREE ACCESS
    Recent analyses of synovial fluid in the temporomandibular joint have confirmed the presence of various markers such as interleukin-1β(IL-1β). Hyaluronic acid (HA) has been reported to be effective effects in the management of temporomandibular joint disorders. However, the effects of IL-1β and HA on chondrocytes are not clear.
    This study was performed to investigate the effects of IL-1β and HA on collagen gene expression in chondrocytes of mouse mandibular condyle. Collagen gene expression was analyzed on the basis of mRNA levels by the RT-PCR procedure. We found that IL-1β significantly decreased the level of type II collagen mRNA and increased the level of type X collagen mRNA. HA significantly increased the levels of types II and X collagen mRNA. In additions, HA inhibited the IL-1β-induced decrease in type II collagen mRNA and increase in type X collagen mRNA.
    The results suggested that HA promotes the repair of cartilage and inhibits IL-β-induced damage of cartilage.
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  • Yasuhiro OKAZAKI, Hideaki KAGAMI, Tamami HATTORI, Sumiyo HISHIDA, Tosh ...
    1999 Volume 45 Issue 2 Pages 73-79
    Published: February 20, 1999
    Released on J-STAGE: July 25, 2011
    JOURNAL FREE ACCESS
    We ligated the duct of the rat submandibular gland and to prepare a model of submandibular gland atrophy. Two weeks after ductal ligation, the effects of bFGF on the regeneration of salivary gland tissue were investigated.
    1) Two-week ductal ligation caused severe atrophy of acini, and the glandular weight decreased up to 26%.
    2) Isoproterenol and bFGF administration significantly increased the number of PCNApositive cells.
    3) The proliferative effect of bFGF in vivo was peak at a dose of 1 ng/gland.
    4) Although isoproterenol stimulated cell proliferation, especially in the ductal region, bFGF promoted the proliferation of various kinds of cells, including acini.
    5) Two weeks after bFGF administration, the glandular weight increased and the tissue recovered, especially in the acinic region, as compared with the control group. Our results indicate that bFGF may accelerate the regeneration of atrophic salivary gland tissue.
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  • Hiroshi HASEGAWA, Makoto KANO, Eijyu SATOH, Masatoshi USUBUCHI, Yuuji ...
    1999 Volume 45 Issue 2 Pages 80-87
    Published: February 20, 1999
    Released on J-STAGE: July 25, 2011
    JOURNAL FREE ACCESS
    Electrochemotherapy (ECT) or electrical impulse chemotherapy, proposed by Okino and Mohri and Mir et al., is a new technique that enhances the antitumor effect of various anticancer agents by delivering high-voltage electrical impulses to tumors. Recently, several reports have described the effectiveness of ECT against tumor cell lines. However, few experimental studies have evaluated the optimum electrical conditions for ECT. We designed this study to determine the optimum electrical conditions for ECT against rat squamous cell carcinoma in vitro and in vivo.
    SCC158 cells, a rat squamous cell carcinoma cell line, were cultured in Dulbecco's MEM with several concentrations of bleomycin (BLM) for 72 hours. The BLM concentration that inhibited cell growth by 50%(BLM-IC50) was determined by MTT Assay. Tumor cells in the media containing BLM-IC50×10-1, BLM-IC50×10-2, or BLM-IC50×10-3 recieved electrical pulses (200-1400V/cm, 0.1msec, 1-8 pulses) and were sequentially recultured for 72 hours. The surviving fraction of tumor cells was analyzed.
    SCC158 cells were transplated into the tongue of Fischer rats. The rats recieved electrical pulses (125-1200V/cm, 0.1msec, 8 pulses) 30min after the administration of BLM. The antitumor effect of ECT on these tumors and damage to the surrounding normal tissue were then evaluated histologically.
    The anticancer effect of ECT appeared when the voltage was more than 600V/cm. Futhermore, ECT of 1000-1200V/cm enabled the BLM concentration to be reduced to as low as 1/100 the BLM-IC50 to obtain a similar anticancer effect in vitro. In addition, the anticancer effect of ECT was strongest when the voltage was 1000V/cm or more; however, the surrounding normal tissue within the electrical field was seriously damaged in vivo.
    In conclusion, ECT is expected to be useful in the treatment of human oral cancers, and the optimum voltage is considered to be 1000V/cm or more. It is hoped that problems related to damage to normal tissue will be resolved by future investigations.
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  • special reference to the expression of transforming growth factor-α and epidermal growth factor receptor
    Akio KANO, Kenji MOGI
    1999 Volume 45 Issue 2 Pages 88-94
    Published: February 20, 1999
    Released on J-STAGE: July 25, 2011
    JOURNAL FREE ACCESS
    Sixty-four cases of oral leukoplakia were examined immunohistochemically by the ABC method with monoclonal antibodies to transforming growth factor alpha (TGF-α) and epidermal growth factor receptor (EGF-R) to study the correlation between epithelial dysplasia of epithelial cells and localization.
    Method: Specimens removed surgically and by biopsy were fixed according to convevtional methods, embedded in paraffin, thinly sliced, and H-E stained for histopathological study. The specimens were stained immunohistochemically by the ABC method with monoclonal antibodies to observe the localization of TGF-α and EGF-R.
    Results: The localization of TGF-α was often observed evenly in all layers of the epidermis in both normal mucosa and leukoplakia without epithelial dysplasia, whereas in leukoplakia with epithelial dysplasia it was often found irregularly in all layers of the epithelium except for the basal layer. In nomal mucosa and leukoplakia, the localization of EGF-R was frequently found in all layers of the epithelium except for the basal layer.
    We consider immunohistochemical studies of the localization of TGF-α to be useful for the evaluation of epithelial dysplasia in leukoplakia.
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  • Katsuhiro NAGASHIMA, Ritsuo TAKAGI, Hideyuki HOSHINA, Akihiko IIDA, Ta ...
    1999 Volume 45 Issue 2 Pages 95-99
    Published: February 20, 1999
    Released on J-STAGE: July 25, 2011
    JOURNAL FREE ACCESS
    Twenty cases of upper gingival carcinoma and 13 cases of hard palate carcinoma were evaluated clinicopathologically. In addition, the relationship between surgical intervention and outcome was also studied.
    All upper gingival carcinomas were identified as squamous cell carcinoma histologically. In the hard palate, there were various kinds of carcinomas arising mainly from the salivary glands (10/13 cases). Furthermore, almost all cases in the hard palate were welldifferentiated mucoepidermoid carcinomas, classified into the early stages of T 1 or T 2.
    The 5-year cumulative survival rate of the patients with hard palate carcinoma was 100%. In contrast, the 5-year cumulative survival rate of patients with upper gingival carcinoma was 50.2% among all cases, 66.7% among 9 stage II+III cases, and 32.3% among 11 stage IV cases.
    Surgical intervention involving tooth extraction in the tumorous area had been done in eight patients with upper gingival carcinoma before their first visit to our institute. Of these patients, seven (87.5%) were classified as T 4, and cervical lymph node metastases were confirmed histologically in five (62.5%). Careful follow-up in thus necessary in such patients.
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  • experimental study based on the morphology of major fragments in fractures
    Akihiro IKAI, Masashi SUGISAKI, Young-Sung KIM, Haruyasu TANABE
    1999 Volume 45 Issue 2 Pages 100-105
    Published: February 20, 1999
    Released on J-STAGE: July 25, 2011
    JOURNAL FREE ACCESS
    An intramedullarpin inserted from the base of the mandible in used for open reduction of fractures of the mandibular condylar prosess. However, depending on the morphology of the mandible, there is some risk of mandibular canal injury during insertion. In most cases, the condylar process in dislocated. It is therefore difficult to estimate the risk of mandibular canal injury during insertion when the method we described previously, which is based on the tangent of the posterior border of the mandibular ramus, is used preoperatively. In this study, instead of using a tangent that is difficult to measure in fractures, we deduced a virtual standard plane of the mandibular ramus from the morphologic characteristics of the major fragment and used it to estimate the risk of mandibular canal injury. Namely, we calculated the angle of the posterior border of the mandible from a virtual standard plane, using the x-ray films of 37 Japanese dry skulls, and estimated the probability of injury. The results were as follows.
    When the pin was aimed towards the superior part of the condylar neck, there was a risk of mandibular canal injury on insertion when the angle between the posterior border of the mandible and the mandibular plane was 125 degrees or more. When the pin was aimed towards the inferior part, there was a risk when the angle was 105 degrees or more. When the angle was 130 degrees or more, the risk of the injury was 4 percent when the pin was aimed towards the superior part of the condylar neck, whereas the risk of injury was 25 percent when it was aimed towards the inferior part of the condylar neck. An acceptable cutoff value of the angle of insertion when the pin was amied towards the superior part of the condylar neck was 125 degrees, and that when the pin was amied towards the inferior part was 120 degrees.
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  • Hiroki MIYATE, Mitsumasa YOKOTA, Kyuuji FURIHATA, Yaeko NAKAMURA, Mana ...
    1999 Volume 45 Issue 2 Pages 106-115
    Published: February 20, 1999
    Released on J-STAGE: July 25, 2011
    JOURNAL FREE ACCESS
    When severe mandibular asymmetry is corrected by sagittal splitting ramus osteotomy (SSRO), the distal bone fragment pushes the proximal bone fragment on the side of deviation. In some patients, temporomandibular joint dysfunction (TMD) is caused by rotation of mandibular condyle. Although intraoral vertical ramus osteotomy has the advantage of correcting mandibular asymmetry, long-term intermaxillary fixation (IMF) is necessary, because rigid fixation is impractical. Various other complicated methods are used for operation, but a simple procedure and a short duration of IMF are preferred.
    We used intraoral inverted L ramus osteotomy with SSRO to correct 14 cases of mandibular asymmetry. All patients had good facial symmetry. This procedure was able to decrease the rotation of the condyle on the side of deviation, and semirigid fixation with a miniplate was possible. Consequently, no patient had TMD postoperatively, and the duration of IMF was shortened.
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  • Yasunori OKUBO, Kazuhisa BESSHO, Saiseki Ko, Noriko KANEDA, Shinsuke H ...
    1999 Volume 45 Issue 2 Pages 116-118
    Published: February 20, 1999
    Released on J-STAGE: July 25, 2011
    JOURNAL FREE ACCESS
    Although some doctors use kampo medicine clinically, it has not yet achieved general acceptance. This seems to be partly caused by the paucity of reports on the effects of kampo medicine as evaluated by basic research. We performed a comparative study of the analgesic effects of kaikyushokushou-to (300, 600, 1200mg/kg, per os), saline (negative control), and diclofenac sodium (poistive control) in mice. All doses of kaikyushokushou-to had an analgesic effect. These results suggest that kaikyushokushou-to can be used for analgesic purposes clinically.
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  • Tsukasa TSUJI, Masaharu SONOBE, Makoto NOGUCHI, Geniku KOHAMA
    1999 Volume 45 Issue 2 Pages 119-121
    Published: February 20, 1999
    Released on J-STAGE: July 25, 2011
    JOURNAL FREE ACCESS
    Extranodal lymphoma of the oral cavity is relatively rare. It is often difficult to distinguish from inflammatory changes. A case of non-Hodgkin's malignant lymphoma of the mucolabial fold of the maxilla arising in a 36-year-old woman in whom the disease was definitively diagnosed on the basis of rearrangement of the immunoglobulin gene is reported. Routine pathological examination and immunohistochemical studies of the lesion could not prove malignant lymphoma. Additional examinations with Southern-blot disclosed clonality of B-cells. The patient received combination chemotherapy with CHOP and radiotherapy. There has been no evidence of recurrence of the lesion as of 10 months after the first visit.
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  • Taichiro SAZUKA, Katsumi NAITO, Kenji HASHIMOTO
    1999 Volume 45 Issue 2 Pages 122-124
    Published: February 20, 1999
    Released on J-STAGE: July 25, 2011
    JOURNAL FREE ACCESS
    Phlegmon in the cheek due to dental infection is frequently observed in clinical practice. We report a patient with a brain abscess that appeared to result from extension of phlegmon in the cheek. A 30-year-old man visited our hospital because of swelling in the right cheek. He was given a diagnosis of phlegmon in the cheek, caused by infection in the right man dibular second premolar and first molar, and admitted. Blood examination on admission revealed a leukocyte count of 22, 300/mm3 and a CRP of 30.4mg/dl.
    After intravenous drip infusion of antibiotics, the swelling in the cheek disappeared, and the findings of blood tests became normal. However, on the 15th disease day, tetany developed, and a CT scan revealed a brain abscess. The brain abscess improved after infusion of a higher dose of antibiotics, and he was discharged on the 63rd disease day. As the infection route, hematogenous infection from phlegmon in the cheek was strongly suspected.
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  • Kazuo UCHIDA, Masato KOIE, Tomoyasu NAKAMURA, Yousuke JINNO, Yoshinori ...
    1999 Volume 45 Issue 2 Pages 125-127
    Published: February 20, 1999
    Released on J-STAGE: July 25, 2011
    JOURNAL FREE ACCESS
    Gardner's syndrome is a disease characterized by the triad of polyposis coli, osteoma, and a soft tissue tumor. However, Fader et al. added supernumerary teeth and impacted teeth as the fourth symptom in 1962.
    We describe a case of complete Gardner's syndrome. The patient was a 23-year-old woman with a soft tissue tumor in the occipital region, the right upper eyelid region, and the epidermis of the back. In the oral cavity, latter stage deciduous teeth and loss of permanent teeth were evident. X-ray findings revealed a ground glass appearance in the maxilla and mandible as well as multiple odontoma, impacted teeth, and osteoma in the frontal ethmoid sinus, the occipital region, and both femur bones.
    In addition, the patient's mother had a history of Gardner's syndrome. Gastrointestinal endoscopy revealed multiple polyps in the stomach, colon, and rectum.Based on the above findings, the patient was given a diagnosis of complete Gardner's syndrome.
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