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Volume 39, Issue 2
Displaying 1-17 of 17 articles from this issue
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Part 1 cDNA cloning of murine bone morphogenetic protein by polymerase chain reactionAkira NIFUJI, Shinichiro OIDA, Masato TAMURA, Satoshi YAMAGUCHI, Kohsu ...1993 Volume 39 Issue 2 Pages 103-109
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSA cDNA clone of murine bone morphogenetic protein (BMP) was obtained by polymerase chain reaction, using oligonucleotides as primers derived from human BMP2 and BMP4. Messenger RNAs were isolated from neonatal murine tissue and used for the synthesis of cDNA with reverse transcriptase.
Specifically amplified cDNAs were cloned into bluescript plasmid and subjected to sequencing. One clone which partially encoded murine BMP2 was obtained. The nucleotide sequence, 554 bp in length, shared 88.6% identity with that of human BMP2 and the deduced amino acids including the mature, biologically active protein exhibited 96.2% homology. Within the C-terminal 114 amino acids of the protein, seven cysteine residues were completely conserved which is characteristic of TGF-β superfamily. Substitution of only three amino acids was found when compared with the corresponding human sequence. Isolation of the full length cDNA is now in progress.View full abstractDownload PDF (776K) -
Satoru SHIOTA, Choichiro FUNAMOTO, Kazuyoshi TAKAZAWA, Sanshiro OHIRA, ...1993 Volume 39 Issue 2 Pages 110-115
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSAttention has already been directed to the antitumor action of plant glycosides, but the involved mechanism of action is unknown.
In this experimental study, the effect of glycyrrhizin in inhibiting growth of a cultured cell of salivary gland tumor induced by 20-methylcholanthrene (cultured S. G. tumor cell) was investigated.
The following results were obtained:
1) Glycyrrhizin inhibited the growth of the cultured S. G. tumor cell.
2) Glycyrrhizin inhibited growth at concentrations over 0.4mg/ml. Growth inhibition at 0.4-0.6mg/ml of glycyrrhizin was comparable to that at 0.5 μg/ml of actinomycin D.
3) Histopathologically, cultured S. G. tumor cells treated with glycyrrhizin showed signs of degeneration and destruction such as cell atrophy and pyknosis.
4) Ultramorphologically, microvilli and branching processes of the cell surface of the cultured S. G. tumor cells were poorly developed and the nucleus was apparently decreased in size.
5) Flow cytometric analysis of cultured S. G. tumor cells after 24 hr. of exposure to glycyrrhizin revealed that glycyrrhizin treatment led to marked accumulation of cells in G1phase, suggesting that growth inhibition was the result of a block during G1 which did not permit the cells to enter S phase.View full abstractDownload PDF (6022K) -
Hiroyuki IWATA1993 Volume 39 Issue 2 Pages 116-126
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSThe concentrations of the six kinds of muscle fiber type-specific proteins (B-subunit of creatine kinase, CKB; S-100a0;αB-crystaline;β-subunit of enolase, β-enolase; M-subunit of creatine kinase, CKM: parvalbumin) were measured in the rat tongue, fast muscle (ensor digitorum longus) and slow muscle (soleus) in the hindlimb. The effect of the complete severance of the lateral hypoglossus nerve was also examined in the tongue.
The following results were obtained:
1) The concentrations of CKB, localized mainly in type I fibers in the tongue, were extremely high, while those of S-100a0 and αB-crystaline, localized mainly in type I fibers in the tongue, were comparable to the values in the EDL.
2) The concentrations of β-enolase, CKM and parvalbumin, localized mainly in type II fibers in the tongue, were comparable to those in the SOL.
3) After denervation of the tongue, the concentrations of β-enolase and CKM decreased, whereas those of CKB and parvalbumin increased until the 6th postopeative week. After the 12th week, there was a tendency to return to the normal level, possibly due to recovery of muscle function associated with reinnervation.
4) The concentrations of S-100a0 and αB-crystalin were not affected by denervation.
It was concluded that: 1) The tongue is composed of mixed fiber type, exhibiting type I fiber factors of muscle fiber type-specific proteins; 2)β-enolase, CKB, CKM and parvalbumin can be good markers of tongue muscle condition after nerve injury.View full abstractDownload PDF (1210K) -
Measurement of impact-produced strain and accelerationMasahito INADA, Jun SHIMADA1993 Volume 39 Issue 2 Pages 127-142
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSFactors involved in the mechanism of mandibular fracture were studied using dentate human air-dried skulls with mandibles by measuring the acceleration of impact with a threeaxial acceleration convertor and the impact-produced strain with monoaxial strain gauges.
The following results were obtained:
1) Application of impact onto the medial region of the mentum suggested a high probability of mandibular fracture to occur at the mandibular neck when the mouth was occluded, and intracapsular fracture of the temporomandibular joint to occur by compression of the condylar process when the mouth was open.
2) Application of impact in the direction of the mentum to the mandibular head suggested a high probability of mandibular head fracture to occur by bidirectional (mediolateral and anterior) buckling of the mandibular head when the mouth was occluded, and mandibular neck fracture to occur by transverse buckling mediolaterally to the neck when the mouth was open.
3) Application of impact onto the mandibular body suggested a high probability of fracture to occur at the site of impact application by buckling, and fracture of the mandibular head to occur by mediolateral buckling of the head, regardless of whether the mouth was occluded, open or rigidly fixed. The probability of mandibular head fracture by crushing was considered to be low when the mouth was occluded or open.
4) Application of impact onto the mandibular angle suggested a high probability of mandibular fracture to occur at the wisdom tooth by bending, regardless of whether the mouth was open, occluded or rigidly fixed.
5) Application of impact in the direction of the mandibular angle to the mandibular head suggested a high probability of mandibular neck fracture, regardless of whether the mouth was open, occluded or rigidly fixed. Fracture of the mandibular head by compression was considered to be less likely when the mouth was open than when the mouth was occluded or rigidly fixed.View full abstractDownload PDF (3344K) -
Histological findings around the implantsYoshiro MATSUI, Kohsuke OHNO, Mariko NAKAGAWA, Takashi OKADA, Tatsuo S ...1993 Volume 39 Issue 2 Pages 143-150
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSThc purpose of this study was to compare the tissue response to titanium implants coated with hydroxylapatite by flame spraying (AFC implants) and conventional titanium (Ti) implants. Eight beagle dogs were studied. Three months after the extraction of their premolars, two AFC implants were inserted in one side of the mouth and two Ti implants were inserted in the other. The animals were sacrificed after 2 weeks and 1, 3 and 6 months. Non-decalcified sections, 15 μm in thickness, were made with a cutting-grinding system and stained with toluidinc blue for histological examination.
The following results were obtained:
1) Direct bone bonding to the AFC surface occurred earlier than direct bone contact to the Ti surface.
2) Comparable bone remodelling occurred around both types of implants at 6 months postoperatively.
3) Non-cellular solubilization of HA granules and phagocytosis of HA granules by mononuclear and multinuclear cells were detected around the bone-free HA surface. At the apex of sonic AFC implants, the coating layer had disappeared at 6 months postoperatively.
4) Mononuclear cells were observed on Ti implants. The number of these cells diminished 3 months after implantation.
5) These results suggested that AFC implants had the advantage of earlier stabilization by bone than Ti implants. However, further studies are warranted on reactions to the HA coating during long-term application.View full abstractDownload PDF (11260K) -
Mitsuhisa OKITSU, Jun OHNO, Kouichiro NAGAMINE, Jun SHIMADA, Nobuo UTS ...1993 Volume 39 Issue 2 Pages 151-157
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSThe immune response plays an important role not only in defense against various infections from the external environment, but also in the homeostasis of the internal environment. However, advancing age is accompanied by declining immunocompentency.
Langerhans cells are known to be antigen presenting cells residing in the epithelium of human oral mucosa. Age-related changes in the numbers of CD1a and HLA-DR positive Langerhans cells were investigated in normal human gingival mucosa using an immunohistochemical technique. Langerhans cells reactive with both antibodies were located in the parabasal to upper spinous layers. At all ages studied, the number of CD1a positive cells was 10-20% higher than that of HLA-DR. In particular, the number of CD1a positive Langerhans cells in the gingival mucosa was significantly different between subjects in their twenties and those in their sixties (P<0.05). On the other hand, significant differences in the number of HLA-DR positive cells was confirmed between subjects in their twenties and those in their forties or above (P<0.05).
This suggests that the local immume response of human gingival mucosa associated with Langerhans cells may decline with aging.View full abstractDownload PDF (1908K) -
Hiroshi MURASE1993 Volume 39 Issue 2 Pages 158-164
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSI carried out restriction endonuclease analysis of DNA using BamHI and Sall for herpes simplex virus type 1 (HSV-1) strains isolated from saliva and tears, body fluids both supplied by the trigeminal nerve in an individual.
The samples consisted of 32 strains which were isolated from both saliva and tears or from bilateral tears simultaneously. They were comprised of 19 strains from 8 oral surgical patients with malignant tumors and 13 strains from 6 patients who underwent opthalomological surgery.
20 out of the 32 strains (62.5%) showed different DNA cleavage patterns with BamHI and Sall. Different strains of HSV-1 were excreted in the saliva and tears or in the bilateral tears from 9 out of 14 patients (64.3%). In the remaining 12 strains from 5 patients, the DNA cleavage patterns by Sall showed similar patterns.
These results suggested the presence of a latent dual infection of HSV-1.View full abstractDownload PDF (2366K) -
Natsuki SEGAMI, Ken-Ichiro MURAKAMI, Hideo HOSAKA, Yoshiyuki MORIYA, K ...1993 Volume 39 Issue 2 Pages 165-171
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSOsteoarthrosis associated with findings of avascular necrosis of the mandibular condyle on magnetic resonance imaging was clinically evaluated. The subjects consisted of 5 female unilateral cases, with an average age of 55.4 years who had a mean duration of illness of 15.2 months. The symptoms were a mean interincisal opening distance of 32.8mm with arthralgia in mild to moderate degree, and joint noise (crepitation) in all joints. MRI documented decreasing signal intensity of the condyle with 2 joints in high degree and 3 joints in moderate, as well as variable low signal density of the articular eminence, associated with concomitant anterior disk displacement. X-ray showed deformity of the condyle and eminence. The operative findings disclosed disk rupture and comparatively smooth cartilage with minimum remodeling of the condylar surface; however, avascular evidence was suggested when corticotomy was performed. Four joints underwent diskectomy, condylar shaving, auricular cartilage grafts and corticotomy of the condyle. Arthroscopic corticotomy was applied for 1 joint with mild symptoms. Postoperative symptoms improved to mean opening distance of 41. 4mm and the disappear of arthralgia in 4 joints. The surgical outcome was excellent in 3 joints and good in 2 joints. Follow-up MRI in 2 cases disclosed increasing signal intensity, which suggested recovery of condylar vascularity following corticotomy.
It is suggested that the low-signal intensity on MRI in osteoarthrosis might indicate avascular lesions of the condyle, secondary to degenerative change. Histologic investigations are required to evaluate avascular necrosis of the temporomandibular joints.View full abstractDownload PDF (6784K) -
Shizuka INUKAI, Shinobu MIYAUCHI, Yoshiaki KAYADA, Koji YOSHIGA, Kazua ...1993 Volume 39 Issue 2 Pages 172-174
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSA case of radiation-induced osteosarcoma is reported. A 43-year-old woman developed mandibular osteosarcoma 28 years after receiving radiotherapy with 60Co (dose unknown) for hemangioma in the oral and neck region.
Hemangioma remained in a wide area surrounding the malignant tumor, making it inoperable. Chemotherapy was selected as palliative treatment, also considering the patient's quality of life. Despite chemotherapy with cyclophosphamide 3, 400mg and radiotherapy with 60Co 30 Gy, the tumor grew rapidly in 4 months, and 120mg of adriamycin and 750mg of carboplatin were additionally administered. It was impossible to control tumor progression and the patient died of lung metastases in July 1991.
The patient was regarded to have suffered a radiation-induced malignant tumor judging from the criteria outlined by Cahan et al., Sato et al. and Sakai et al.View full abstractDownload PDF (2425K) -
Izumi YOSHIOKA, Toshihiro KIKUTA, Tomio SETO, Takeshi NAKASHIMA, Iwao ...1993 Volume 39 Issue 2 Pages 175-177
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSPleomorphic adenoma is a common neoplasm in the oral cavity. This tumor occurs most frequently at the parotid gland in the major salivary gland and at the palate in the minor salivary gland. This tumor rarely develops at the lip. Usually, it appears at the age between 2nd and 5th decades; few cases occur in childhood.
We encountered a case of pleomorphic adenoma on the upper lip of a child. The patient, a 7-year-old boy, visited our clinic with a mass on the upper lip.
There have been 59 cases of pleomorphic adenoma on the upper lip documented in Japan (1961-1991). This patient is the youngest of the 59 cases.
The clinical features of this case are presented along with a review of the literature on pleomorphic adenoma.View full abstractDownload PDF (2908K) -
Takeshi NAKASHIMA, Tadahiro NODAI, Haruhiko FURUTA, Iwao HARA1993 Volume 39 Issue 2 Pages 178-180
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSA 10-month-old girl was referred to our hospital for a mass on the ventral surface of the tongue. The mass was removed surgically.
Histopathological examination of the lesion indicated an “epidermoid cyst” in some sections. However, the cyst wall was continuous with the epithelium of the ventral surface of the tongue, and the multiple-layered squamaus epithelium cells of the wall contained goblet cells. In addition there were abundant minor salivary glands and excretory ducts around this leasion. The lesion was diagnosed as a keratin-filled peudocyst arising in the excretory ducts of the minor salivary gland.View full abstractDownload PDF (3407K) -
Nobuo TAKANO, Yoshinobu KISHINO, Hitoshi OHHATA, Chikara SAITO, Takash ...1993 Volume 39 Issue 2 Pages 181-183
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSAngiosarcoma, a malignant vascular tumor, is very rare as a primary focus in the oromaxillary region. We recently encountered a case of angiosarcoma arising from the mandible in a 7-year-old girl. The tumor was treated with radiotherapy, surgery and chemotherapy but death occurred about 3 years after initial presentation. Only 2 cases, including ours, of angiosarcoma in the mandible have been reported in Japan.View full abstractDownload PDF (3505K) -
Makoto ISOBE, Hidenori MATSUSHITA, Masatami KATO, Naoko MATSUO, Tetsuo ...1993 Volume 39 Issue 2 Pages 184-186
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSCandidiasis is a infectious disease caused by Candida albicans, and clinicaly classified into acute pseudomembraneous, granulomatous and chronic hyperplastic candidiasis. The cause of candidiasis has been ascribed to weakened resistance of the host combined with increased pathogenicity of Candida albicans.
We experienced a case of severe oral candidiasis which repeatedly recurred over a period of 18 years. As one of the local factors of recurrence the patient had a fissured tongue, which was treated by excision of the tongue groove and plastic surgery of the dorsum of the tongue.
Consequently, there have been no signs of recurrence in the operative region of the tongue 1.5 years postoperatively. We recognized importance of the local factors in contracting candidiasis.View full abstractDownload PDF (2642K) -
Tohru OHTSUKA, Yukihiko KINOSHITA, Takatoshi TABATA, Kaizo SHIMURA, No ...1993 Volume 39 Issue 2 Pages 187-189
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSThis paper reports a basal cell ameloblastoma affecting a 66-year-old man whose chief complaint was swelling of the left maxillogingival region.
Buccal cavity examination showed an edentulous jaw with an elastic soft bulge and a tiny round ulcer in the left maxillary tubercle. X-ray examination indicated comparatively clear-cut bone resorption in the left maxillary tubercle. Exploratory excision resulted in a histopathological diagnosis of ameloblastoma, which prompted us to perform left partial maxillectomy. One year and 7 months after the operation, relapse occurred across the pterygoplatine fossa towards the proper nasal cavity. Under these conditions, maxillectomy was reperformed.
Histopathological examination of the removed portion of the maxilla showed the presence cells which principally resembled basal cells. These basal-like cells were partly continuous with the mucous epithelium and became increasingly diffuse with increasing depth.View full abstractDownload PDF (2229K) -
Hiromi HIROHATA, Koujiro ONIZAWA, Toshiyuki HAGIWARA, Hiroshi YOSHIDA, ...1993 Volume 39 Issue 2 Pages 190-192
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSA rare case of neuroendocrine carcinoma arising in the lateral border of the tongue is presented.The patient was a 76-year-old malc complaining of swelling of the tongue and the upper cervical rcgion.
The tongue lesion was histopathologically diagnosed as an anaplastic carcinoma, and showed positive reactions to keratin, CEA, EMA, Lcu-7 and NSE in immunohistochemical staining. Based on these findings, the tumor was finally diagnosed to be a neuroendocrine carcinoma. The neck lesion was suspected to be caused by metastases of the tongue carcinoma. Irradiation was effective, resulting in remarkable reductions in the size of both the tongue and neck lesion.View full abstractDownload PDF (3755K) -
Masahisa NOMURA, Shingo INOUE, Setsuko FUKUDA, Takenori ISHIKAWA, Tsun ...1993 Volume 39 Issue 2 Pages 193-195
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSThe transfer of cefotiam-hexetil (CTM-HE) into human serum, gingiva and removed-tooth socket after oral administration of 200mg CTM-HE was studied with time in 30 patients, consisting of 15 males and 15 females.
The results were as follows:
1) Serum concentrations at 30, 45, 60, 120 and 180 minutes after CTM-HE administration were 0.31±0.22, 0.62±0.76, 0.66±0.52, 1.00±0.38 and 0.84±0.39μg/ml, respectively.
2) CTM-HE concentrations in removed-tooth sockets ranged from 0.10-1.60μg/ml, and the ratios of removed-toothsocket to serum concentrations were 0.1-1.83 during 30-360 minutes after oral administration.
3) CTM-HE concentrations in gingival tissues were 0.32-2.60μg/g, and the ratios of gingiva to serum concentrations were 0.23-1.89 during 30-360 minutes after oral administration.
Based on the above data, CTM-HE is expected to be clinically useful for oral infections.View full abstractDownload PDF (270K) -
Akihiro KANEKAWA, Shunsuke UEMURA1993 Volume 39 Issue 2 Pages 196-198
Published: February 20, 1993
Released on J-STAGE: July 25, 2011
JOURNAL FREE ACCESSThe purpose of this investigation was to determine the type and prevalence of oral soft tissue injuries in children examined in a hospital emergency room and our department. The leading cause of the oral soft tissue injuries was falls, accounting for 61.8% of all injuries. The male-to-female ratio of the injuries was 2: 1, and the peak age was 1 year old. Laceration of the superior labial frenum was the most predominant injury, accounting for 27.0% of all injuries. The peak month for the injuries was June. Emergency treatment under general anesthesia was done in 11 cases, and 12 cases were hospitalized for a few days.View full abstractDownload PDF (329K)
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