Measuring the quantity of egg albumen and guinea pig's serum adsorbed on to silica gel and quartz powder by means of Nessler's Method, the author obtained the following results:
1) Silica adsorbs protein on to its surface.
2) Within some limits, the higher concentration of the protein is, the more protein is absorbed.
3) The quantity of adsorbed protein is affected by “pH” and reaches its maximum around “pH 4”. The quantity decreases gradually in the acid side, while rapidly in the alkali side.
4) Guinea pig's serum is adsorbed more than egg albumen.

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To investigate how silica was excreted when it was administrated to a guinea pig by the abdominal cavity injection, the auther has measured the quantity of the silica in the blood and urine by means of Shinjo's method, and obtained the following results:
1) 1.5cc of 4% hydrofluoric acid could remove the interference of phosphorus, arsenic and iron in the blood and urine.
2) The rate of recovery of silica by this colorimetric method is about 90%.
3) When silica gel is injected into the abdominal cavity of a guinea pig, it is excreted rapidly in the urine, and the concentration is parallel to the concentration in the blood.

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The author refined the yeast polysaccharide from the dry yeast by means of Hioka's method, and experimented on the variation of its antigen activity in the refining process, the relation between its concentration in the blood and that in the urine, and its placental permeability, employing a guinea pig given the refined yeast polysaccharide by the abdominal cavity injection or the intravenous one.
The anti-yeast rabit serum, (the antigen titer by Uhlenhuth's Method being 1:2, 500, 000), was used for proving the yeast polysaccharide in the blood, the urine and the amniotic fluid, and Uhlenhuth's Method was employed for the measuring. The author could clarify the following points:
1) In case of refining the polysaccharide from the yeast, the degree of the antigen activity in the last process is 100 times as much as that of the extract by the physiological saline.
2) The yeast polysaccharide given to a guinea pig by the injection is rapidly excreted into the urine.
3) The yeast polysaccharide in the blood has shown the highest concentration for 4 hours since the injection, and thereafter it begins to decrease rapidly, to be found no more in the blood on the 6th day.
4) The yeast polysaccharide in the urine has shown the highest concentration since the injection, and thereafter it decreases a little less rapidly than that in the blood, to be found no more in the urine on 6th or the 7th day.
5) There is the proportional relation between concentration of the yeast polysaccharide in the blood and that in the urine.
6) In case of giving the yeast polysaccharide to a pregnant guinea pig by the injection, the concentration of the yeast polysaccharide in the embryo's serum, which is transmitted from its mother, is 1/10-1/20 of that of the mother.
7) The yeast polysaccharide is never transmitted into the amniotic fluid.
8) The length of pregnancy has no relation to the degree of the yeast polysaccharide transmission to embryos.

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Reports about the passive anaphylaxis using the yeast polysaccharide as the antigen are very few. After the Rosenau-Anderson's report, there appeared Axamit's one, but all of these are reports about the active anaphylaxis using the yeast cell and its crude extract.
In regard to the passive anaphylaxis using the yeast polysaccharide as the antigen, only Kesten-Mott made a report after Tomcsik-Kurotchkin. So far as I know, there is no such report in our country, either. Though in these reports the minimal lethal antigen dose is referred to, the minimal lethal sensitizing dose is not referred to at all.
The author made an experiment on the passive anaphylaxis using the yeast polysaccharide as the antigen in order to recognize its speciality, and obtained the knowledge of the ability of the sensitization by the anti-yeast rabbit serum and the knowledge of the decrease of the complement titer, etc.
Conclusions are as follows:
1) The passive anaphylaxis can be caused by using the yeast polysaccharide as the antigen.
2) The field of its reaction resembles in shape that of the antigen-antibody reaction in vitro at the antibody excess zone.
3) The minimal lethal sensitizing dose is 10 EH in all, in case of injecting the antigen which is suitable to the zone of antiserum used for the sensitization.
4) In case that the dose of the sensitization is 10 EH per 100 gms. of guinea pig's weight, the minimal lethal antigen dose is 1/16 z.
5) The decrease of the complement is not so evident in case of the passive anaphylaxis using a small amount of the antiserum which has the great ability for the sensitization.
6) In the experiment of the passive anaphylaxis, the sensitizing dose has more influence upon the decrease of the complement than the antigen dose.

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It is well known that an experiment on anaphylaxis of isolated organs is possible just as that of a living body. That is, Schultz reported the possibility of anaphylaxis of a guinea pig's isolated intestine like of a living body, Dale also made the same experiment using isolated uterus, and since then, many students have reported on experiments one after another.
But experiments of anaphylaxis of isolated organs by the yeast polysaccharide are scarecely reported, not to speak of passive anaphylaxis, and only Tomcsik-Kurotchkin's is reported. In this Tomcsik-Kurotchkin's report, mention is made of the minimal antigen dose, but neither of the relation between that minimum and the zone of anti-serum used for the sensitization, nor at all of the minimal sensitizing dose.
In order to recognize more satisfactorily the sensitization ability of the anti-yeast rabbit's serum and the activity of the yeast polysaccharide, which are reported above in Part II, the author made an experiment mainly on the passive anaphylaxis of a guinea pig's uterus, and obtained the following conclusions.
1) The passive anaphylaxis of isolated organs can be caused by the yeast polysaccharide as the antigen.
2) The minimal antigen dose for causing this reaction is about 1/4 of the minimal lethal antigen dose for the passive anaphylaxis, and there is not much difference between both sensitizing doses.
3) In case of the constant antigen dose and sensitizing dose, the passive anaphylaxis of an isolated uterus depends greatly on an individual, therefore, this is not a good way for an experiment of defining the limit of reaction.
4) The isolated intestine shows more uniform reaction type than the uterus.

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After having once injected cow's serum antigen toward both a normal rabbit, as well as a rabbit with impediments in the reticuloendotherial function due to ink injection, I examined and have taken observations on such matters as Congored Index, total serum protein density, precipitin value, as well as quantitative changes that happened; which, offered results as follows:
1. In case of a normal one, the total protein density of antiserum, along with γ-Globulin quantity, almost showed a parallel relation with the precipitin changes. In those viccisitudes that have occurred in α- and β-Globulin as well as Congored Index, no definite tendency could be detected.
2. In case of a splenorectomized rabbit, there occurred an increase in Congored Index, accompanied with a decrease in the total serum protein density, albumin, as well as γ-Globulin, on the day that followed the operation. Moreover, an increase has taken place in β-Globulin.
3. In an ink-filled rabbit, there could be seen, within a day of injection, certain increase in the Congored Index, along with a decrease in total serum protein density, albumin or γ-Globulin.
4. In those rabbits who underwent splenorectomy and ink-injection, no serum protein acting parallel with the precipitin changes, as seen in a normal one, could be observed.
5. The precipitin production has proved at its maximum in a normal rabbit, in 20 days after antigen injection; while, in 20-30 days in case of an operated one; and 14 days in the injected rabbit, among these three groups, the injected rabbit showed maximum; next, normal one, and lastly, rabbit who suffered the operation.
6. At 85th days after the injection, of antigen, a decline in precipitin has proved marked in all the three groups, esp., very eloquent in an operated rabbit.
7. From the above results, it may roughly be concluded that a close correlation exists between γ-Globulin and antibody; further, the role of reticuloendotherial system as mother ground for antibody production may be considered with some significance.

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With the use of adult rabbits, the author performed the bonemarrow culture in coverslips and obtained from the rabbits irradiated repeatedly and systemically with 100r of X-ray 24-41 times, and 300 r of X-ray ten times, and 1, 000 r of X-ray once and observed the bone marrow from respective groups during the periods ranging 1-30 days after the irradiation. The results are described in the following.
1) In the group receiving repeatedly and systemically the irradiation of 1000 r of X-ray the relative growth rate, cell density, the wandering velocity of pseudoeosinophils all decreased markedly.
2) In the group given the irradiation of 300 r X-ray, no sign whatsoever could be recognized as regards the relative growth rate, cell density and the wandering velocity of pseudoeosinophils; and the bone marrow presented aplasia.
3) In the group receiving 1, 000 r of X-ray irradiation once only, the relative growth rate, cell density and the wandering velocity of pseudoeosinophils all docreased gradually lower with the lapse of time; and all the values presented their minimum on the tenth day but on the thirtieth day it was found that they were all rather increased.
4) Of the disturbances in the bone marrow due to the X-ray irradiation. in the groups receiving repeated irradiation the cell proliferation was disturbed in direct proportion to those of the cell function. In the group receiving a single irradiation of 1, 000 r, the cell proliferation was disturbed on the second day but it seemed to have a tendency to precede the disturbances of the cell functions. Therefore, it is assumed that in the case receiving a small dose of X-ray irradiation the cell proliferation of bone marrow is disturbed earlier, and the cell functions are diminished later.

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By injecting 3 mc/kg of radio isotope P³² once only and observed for the periods of 1-14 days and to other group 800 μc/kg once and observing for the periods of 1-30 days, the author performed cultures of the bone marrow from these rabbits in coverslips and obtained the following results.
1) In the group irradiated with P³² 3 mc/kg, the relative growth rate, cell density and the wandering velocity of pseudoeosinophils all decreased strikingly; while in the group receiving P³² 800 μc/kg, these values were found all increased. Namely, in the case receiving a large dosage of P³² irradiation, the white corpuscle series of the bone marrow show marked disturbances while in the case subjected to a small dosage of the irradiation it is stimulated.
2) The increase in the cell proliferation in the case irradiated with snch an amount of P³² as to cause stimulation seems to precede the increase in the cell functions.
3) Therefore, judging these results along with those in report I, the cell proliferation seems to be more sensitive to the irradiation than the cell functions.
4) As the cell proliferation tends to be affected prior to the cell functions in the both irradiations so far described, the influences of P³² irradiation on the bone marrow seem not to differ essentially to any great extent from those of X-ray irradiation.

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Using adult rabbits, the author performed the bone-marrow tissue cultures in fluid medium from the rabbits irradiated repeatedly and systemically with 100 r of X-ray irradiation, 24-42 times; 300 r, 10 times; and 1, 000 r once only and observed for the periods of 1-30 days; and obtained the following results.
1) In the group repeatedly irradiated wiht 100 r X-ray, both the rate of increase of erythrocyte count as well as that of hemoglobin count decreased markedly.
2) In the group subjected to the irradiation of 3000 r, the rate of erythrocyte count and that of hemoglobin count likewise decreased markedly.
3) In the group receiving a single irradiation of 1, 000 r, the rate of erythrocyte count and that of hemoglbin count decreased gradually along with the lapse of time, and both values reached their minimum on the tenth day while by the thirtieth day both were found to be at the normal.
4) In the repeated irradiations as mentioned above, the erythropoietic function is disturbed in parallel with the function of hemoglobin synthesis, whereas in the group subjected to a single irraiation of 1, 000 r X-ray, the erythropoietic function seems to be affected more markedly than the function of hemoglbin synthesis. Therefore, in the case receiving smaller doses it is assumed that the erythropoietic function is affected prior to the function of hemoglobin synthesis.

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Adenosin-triphosphate (ATP) was obtained from the skeletal muscle of rabbits and dogs with Lohmann's method as Ba-salt. Before use this was changed to Na-salt which was proved to be pure chromatographically. Using this Na-salt as substrate observation on ATP ase activity were carried out in tissues of the liver, spleen, heart, kedney, duodenum and skeletal muscles as well as in blood and bone marrow of rabbits. Chemical analysis proved that various organs and tissues gave myosin ATP-ase activity showing the values specific to each organ, i.e. about 10γPi per 100 mg. of wet tissues of heart muscle, liver, spleen, kidney and duodenum, 6γPi per 100 mg. in bone marrow, quite a low value of about 1γPi per 100 mg. in circulating blood. In every organ the activity of mitochondrial ATP-ase was as low as 0.9-10.3γ Pi per 100 mg., quite lower than that of myosin ATP-ase; and as for the differences between the values of myosin and mitochondrial ATP-ases in each organ, it was found that the difference was smallest in peripheral blood and greatest in the heart tissue.
As for the practical application in the histochemical study, frozen slices of formalin fixation are thought to be the simplest and best. According to their reactions against activating agents (cysteine) and inhibitory agents (PCMB), ATP-ase acn be divided into four types. The first type is of the typical ATP-ase which is demonstrable only in the frozenslices of cardiac and skeletal muscles, and detected especially in the region of anisotropic disk. This reaction is inhibited by PCMB and reactivated by cysteine. The second type is of those found in frozen slices of basillar part of epithelial cells of renal urinary tubules, liver parenchymal cells and biliary tubules and mooth muscle of duodenel wall. This reaction is inhibited by PCMB but not reactivated by cysteine. The third type is of those appear at the brush border of epithelial cells of renal urinary tubules of paraffin sections. This reaction is markedly inhibited by cysteine but stands unaffected by PCMB. The fourth type is of those detectable in various portions of tissues such as the smooth muscle of arterial media and intima as well as various viscera. The reaction remins unaffected either by cysteine or PCMB in both the frozen and the paraffin sections. The reaction of blood cells is the one that rightly belongs to this type. Reactions observable in paraffin sections are the enzyme actions mainly belonging to the third and the fourth types; and it is believed the reaction due to phosphomoesterase (IIIrd tpye) and the unknown enzymes other than true ATP-ase (IVth type). In the case of the slices embedded in methacryleric resin all activities excepting that of the brush border of epithelial cells of renal urinary tubules are lost. The ATP-ase activity of leukocytes is found chiefly in the cytoplasm and it is detected in matured cells to a marked degree.

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Generally it is accepted that X-ray irratiation in vivo, attacking H₂O molecules, produces OH, O₂H, H₂O₂ etc., and the oxydation invoked by these by-products inhibits the enzymatic activity of the SH group and others. Bearing this point in mind, changes of ATP-ase activities in the liver, spleen, heart, kidney, and peripheral blood as well as bone marrow have been examined along with the lapse of time in the rabbits irradiated with a quite substantial amount of X-ray irradiation, namely, 2, 000γ each. Chemical estimation proved that myosin ATP-ase activities of cardiac muscle and bone marrow are increased immeadiately after irradiation while the activities of all other excepting these two are considerably reduced. The ATP-ase activities of the liver, spleen, kidney, and Peripheral blood increase gradually 1-3 hours after irradiation while on the contrary the activities of cardiac muscle and bone marrow are found to have decreased markedly 3-6 hours afterwards. Later on the activities of the liver, spleen, kindney and peripheral blood decrease again and after 6-12 hours they show the values below the normal, whereas after 12-24 hours, they recover close to the normal value excepting three cases in perihperal blood. The activities of cardiac mucle and bone marrow decrease for the first 3-6 hours but later increasing gradually, they recover almost to the normal 12-24 hours afterwards. However, after 12 hours the activity of bone marrow declining Gradually, continues its descent without ever recovering. Although the activity of mitochondrial ATP-ase behaves more or less in similar manner as that of myosin ATP-ase, its absolute value is lower than the latter observed at the same time and the former has a tendency to fluctuate prior to the latter.
In histochemical observations the intensity of reactions fluctuates rather identically with the fluctuations observed in biochemical study. The reaction of cardiac muscle is markedly reduced for the first three hours after irradiation but it can be somewhat reactivated with cysteine. After six hours the reactions of liver parenchymal cells, biliary tubules, and basillar part of epithelial cells of renal urinary tubules decrease to a certain degree; and this decrease seems to be due to the true ATP-ase activities controlled by the SH group. The reaction of phosphomonoesterase like ATP-ase receiver no conceivable influence with X-ray irradiation. A decrease in the numbers of leukocytic cells is responsible for the striking reduction in the activity of the spleen 12 hours after irradiation. When the liver, kidney and heart in normal conditions are made to react in the absence of substrate, they show extremely slight reaction; whereas after X-ray irradiation, invariably they reveal no such reaction.

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Among the various anti-leukemic agents, nitrogen mustrad and its derivatives have the similar biological effects identical with x-ray inactivating the SH group. Following up the author's former reports, in which a marked fluctuation of ATP-ase activity in various tissues of the x-ray irradiated mice was described, the author reports the effect of NMNO on the ATP-ase activities of the liver, spleen, heart, kidney and duodenum of mice. By a single injection of 1% NMNO, 300 mg/Kg., most of the adult mice died after 6 to 8 hours. Hourly observations revealed the marked fluctuation of ATP-ase activitity in various organs as in the case of x-ray irradiation. The activities both of myosin and mitochondrial ATP-ase decreased slightly in every organ 30 minutes after the injection of NMNO, and then increase slightly reaching nearly the normal level after 3 hours. Further obverations revealed that the transient recovery of activities decreased again, reaching the level of inorganic P contents proper to each organ 7 hours after injection. The histochemical observation revealed also a marked decrease in the activity of ATP-ase. The inhibitory effect of NMNO to the ATP-ase activities was also proved in vitro, adding 10^-2 mol. NMNO in the incubating media. The colchine, a mitotic poison, added in the same media 10^-2 mol. effected only a slight decrease in ATP-ase activity. The incubation with L-cysteine after the treatment wiht NMNO restored the ATP-ase activity to a certain degrree. In the case treated with NMNO a marked decrease in the reactions of liver parenchymal cells, biliary tubules, blood cells of splenic pulp, cardiac fibers, the basillar part of epithelial cells of renal tubules is observable; and reactions of peripheral blood and blood in bone marrow, which received no effect from X-ray irradiation, are reduced markedly. However, when the reaction is made to take place in the substrate loaded with cysteine, only the reactions of cardiac fibers and liver parenchymal cells are restored to a certain degree.

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The author produced splenomegaly in rabbits by the successive injections of non-physiological macromolecular polymers, and obtained the following results.
1) By the successive injections of methylcellulose, polyvinylalcohol, and ovalbumin either intravenously or intraperitoneally for the periods ranging 50-100 days, the author obtained hematologic macromolecular syndrome, marked anemia and thrombocytopenia in the rabbits.
2) Bone marrow picture revealed the inhibition of maturation in the erythroid system.
3) All the groups receiving injections demonstrated splenomegaly and an increase of the blood pressure in the portal vein. Histologic findings of the spleen were the marked proliferation of reticular cells and occasionally the picture which seems to be fibroadenia.
4) Serum iron decreased while serum copper increased on the contrary. However, the depot iron maintained the normal level. In tracing the mechanism of this experimental anemia from the fact that the depot iron did not decrease despite the marked decrease of the serum iron, the author concluded that the principal cause of this experimental anemia lies in the inhibitory action of toxic factor upon the mobilization of depot iron.
The results of this experimental hypersplenism are believed to be identically the same as those of so-called Banti's disease as reported by T. Kobayashi.

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By injecting to normal rabbits the spleen extracts and sera of the rabbits with experimental splenomegaly, the author examined whether or not the toxic factor exists in such extracts and sera, and studied influences of the toxic factor upon the iron and copper metabolisms. The experimental results are described below.
1) A toxic factor was recognized to exist in the spleen and serum of the rabbits recei. ving the injections of methylcellulose, polyvinylalcohol and ovalbumin.
2) By the injection of the substances containing such a toxic factor to the normal rabbits, the value of serum iron became lower while that of copper higher. In studying the direction of action of the toxic factor mainly from the standpoint of the iron metabolism it has been found that despite the normal amount of depot iron, the serum iron decreased markedly. From this fact it is concluded that the anemia is caused by the inhibitory action of the toxic factor on the mobilization of depot iron with resultant interferance with the erythropoiesis in the bone marrow.
3) As mentioned above, the author believes beyond doubt to have confirmed for the first time the existence of a toxic factor in the spleen and serum in the rabbits with experimental hypersplenism and to have clarifed the active mechanism of the toxic factor.

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With bone marrow cultures of the rabbits given successive injections of methylcellulose, polyvinylalcohol and ovalbumin for a long period of time, the author studied the influences of such injections on the tissue growth and the cell migratory power; and obtained the following results.
1) The growth of the bone marrow tissue was clearly suppressed in all the groups tested.
2) The cell migratory power of bone marrow cells was also decreased rather appreciably in every group receiving the injections.
From these results, it is concluded that the toxic factor created by injections acts not only as a causative factor for anemia and thrombocytopenia but it also acts on white cell series, thereby hindering the bone marsow tissue growth and diminishing the cell migratory power as well.

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It goes without saying that reliable and referable results will come from the standardized hearing tests. But standardizing tests of hearing is a difficult problem, especially in the hearing by bone conduction. Why so difficult is it in the bone conduction audiometry? Thhere are two reasons. At first, bone conduction audiometry has more factors to be thought about in testing instruments and manipulations, for example, touching pressure of the receiver and so on. Next, the value which should be considered normal threshold fluctuates on rather wider range by bone conduction comparing with air conduction.
Then, the following points are investigated in this report, i.e., “relations between the touching pressure of the receiver and the bone conduction threshold, ” “suitable masking noise” and “comparison of tests using continuous tone and interrupted tone.”
The ressults obtained are as follows:
1. As the touching pressure increases, bone conduction threshole goes down till it holds constancy. Generally speaking, the value of suitable pressure is 700g/cm².
2. Necessary and tolerant masking noises have to be settled with one ear deaf patients and healthy men. But actual value of masking noise is to be varied in each case and in each instrument.
3. The threshold by interrupted test tone is lower than that by continuous tone. And the use of interrupted tone has many conveniences in practice.

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By oral administration of the eggs of ascaris to guinea pigs and rabbits, the animals were infected, or reinfected, or reinfested after sensitizing them with ascaris emulsion; and the blood picture. the roentgenograms of the lungs as well as histo-pathological findings of respective groups were comparatively studied. The results obtained are described below.
1) In blood picture ebsinophilia has been recognized in all the cases, whereas it is most marked in the cases where the animals are reinfested after the sensitization with ascaris emulsion.
2) When a large amount of ascaris eggs is given in the first administratios the shadows like pneumonia or nodular shadows are revealed in the roentgenograms of the lungs while in the cases of super infect or reinfection only, the appearance of these shadows is on the whole quicker and relatively more nodular shadows are observed as compared with the former. Of the four cases receiving the ascaris eggs after sensitization with the emulsion of ascaris, one case presented a picture of transitory infiltration in the lungs.
3) In the primary infection cases, hyperemia and hemorrhages are generally the main findings at early stage but later on the hypertrophy of alveolar septum can be recognized due to the infiltration of neutrophils, eosinophils, lymphocytes, and moncytes and the hypertrophy and proliferation of alveolar epithelium. In the cases reinfected or reinfected after the sensitization with the ascaris emulsion, the swelling and hypertrophy of blood-vessel wall and demas in the perivascular space are more marked than the former, and likewise the nodular cell infiltration and the appeareance of giant cells are more striking than in the first infection cases. Grannloma formation can also be recognized in the latter.
4) From these findings it has been found that the ascaris allergy plays one of the pathogenetic roles in the transitory infiltration of the lungs.

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By the percutaneous or the oral administration of the eggs of dog-ascaris, the dog, the host specific to and guinea pigs and rabbits, the hosts non-specific to the ankylostoma caninum were infected, or reinfected or reinfected after a prior sensitization with the emulsion of ankylostoma caninum; and roentgenograms and histological findings of the lungs of respective cases were compared. The results are described below.
1) In the cases of non-specific hosts, from 5-6 days after the percutaneous or oral infections, they presented abnormal shadows in the roentgenograms of the lungs, and in the case of reinfection on the fourth or fifth day they revealed bronchial pneumonia-like or spotty or nodular shadows: and all these proved to occur much more quickly in a greater degree than the shadow shown in the cases of the primary infection. In the cases reinfected after the sensitization with the emulsion of ankylostoma the shadow of transitory infiltration in the lungs was recognized. In the specific hosts no changes so marked as those observed in non-specific hosts for the primary infection, but in one of the oral reinfection cases among the specific hosts the shadow of the transitory infiltration in the lungs was recognized.
2) Macrospcapically, in the non-specific hosts receiving the oral or the percutaneous primary infections hemorrhages and hyperemia were main changes and in some grayish white nodules could be observed in the lungs. In the cases of reinfection more marked changes and more numerous nodules were observable than in the former, and in the reinfection cases after the prior sensitization with emulsion, grayish white spotty processes were recognized in the lungs. In the specific hosts pathological changes were slight and grayish white spots could be seen only when reinfected. Histologically, in all cases the changes were more striking when reinfected, namely, swelling of the alveolar septum, the nodular cell infiltration and giant cells were observed around blood vessels and bronchi; and in the specific hosts granuloma formation was recognized.
3) From these findings it seems that the hookworm allergy plays one of the pathogenetic roles for the transitory infiltration in the lungs.

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With the use of the eggs of pig ascaris and the emulsion of pig ascaris, guinea pigs were sensitized and infected, and after either resensitizing or reinfecting these animals, the intracutaneous and the intestianl reactions as well as the macroscopical and the histologic findings were studied. The following are the experimental results.
1) Both the intracutaneous and the intestinal reactions proved to be positive; and the reactions were stronger in the cases infected after sensitization with emulsion of ascaris than in those infected with ascaris.
2) In both the reinfected and resensitized cases, on the second to fourth day macroscopically the hyperemia and cloudiness of the mesentery were the main changes and microscopically hemorrhages, hyperemia and the infiltrations of eosinophils, monocytes and lymphocytes could be seen in the mesentery. On the tenth day they showed cloudiness and swelling and histologically the cell proliferation of serous membrane and edema as well as hypertrophy of the wall of arterioles could be recognized. The reinfected cases revealed granulative inflammations and the infiltrations of giant cells, monocytes and eosinophils. On the twentieth day in some the fusion of mesentery and grayish white scar formation could be recognized and string-like proliferation of connective tissue as well as hyaline degeneration were detected.
3) From these findings it is believed that ascaris allergy is one of the causes of mesenteritis cicatricans.

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In 5 human brains (9 hemisphares) the distribution area of Betz cells were extended in a plane and their distributing density was studied.
1) The extended diagrams showed an irregular elliptic shape in each hemisphare without regard to the directions of the gyri.
2) In case of the same person the diagram was larger in the dominant hemisphare.
3) The distributing density of Betz cells was also larger in the dominant side.
4) Generally the Betz cells were found less in the ridges of the gyri and more in the bottom.
5) Campbell's suggestion that there are 7 groupings of Betz cells was not justified, but the distributing density was not homogenous even in the area of dense distribution.
From the facts above mentioned, if it is considered gyri are made passively when the brain grows in a limited cranium, it is concluded that, the distributing area of Betz cells may be placed posterior to the central sulcus or be hidden in the bottom of the sulci and the gyrus can not be the standared for the determination of localisation of the cerebral areas.

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I have made liver function tests for the 55 patients of lungtuberculosis and compared the results of them with the clinical findings of those patients. The results obtained were as follows:
(1) Two of the liver function tests which agreed well with the clinical findings were the function of reticuloendoterial system and the function of albumin metabolism.
The function of bilirubin metabolism was the next by means of the fall of serum bilirubin.
(2) One of the clinical findings having the most connection with the disturbances of various liver function tests was fever. The next was the acceleration of blood sedimentation rate. The prolongation and aggravation of illness, the existence of cavity, the excretion of bacillus, the circulations spreading, the diminution of L/M ratio and the complication of intestinal tuberculosis etc. were more or less connected with the results of the liver function tests.

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I havc given 20 cc of 5% Glucose with 1 mg% of 3.6-Diamino-Eurfurol 10-Methyl-Acridium Chloride intravenously to the 55 patients of lungtuberculosis once a day for 30 days. The results obtained were as follows:
(1) In general, the various symptoms on the subjective symptoms were improved with this medicine especially were remarkable in benign and middle grade cases.
(2) As for the effect of Acrifuran on the blood findings, they were much improved in benign cases, but they were fifty-fifty of improvement and unchangeability in middle grade cases.
In severe cases, they were unchangeable or some of them showed anenicic.
(3) As for the effect of Acrifuran on the liver function, all of the liver functions in benign cases were improved with Acrifuran. But the function of sugar metabolism in middle grade cases, and of bilirubin metabolism and Albumin metabolism in severe cases remained unimprovable.
In general, Acrifuran gave much improvement on the Antidotal function, the function of reticuloendothelial system and of foreign body exceretion.

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I gave 5% Glucose solution with 1mg% of Acrifuran (3.6-Diamino-Furfurol-10-Methyl-Acridium Chloride) to normal rabbits and the rabbits with benign and severe liver damage caused by the use of Carbon tetrachloride. Furthermore, I made a plug in the reticuloendothelial system by the use of Indian ink after the empose of Acrifuran and then observed the various liver function tests. The results obtained were as follows:
The Antidotal function by the use of phenothiazine methode, the function of reticuloendothelial system by the use Congo Red methode and the function of sugar metabolism by the use of Galactose methods were accentuated and lor improved with Acrifuran, but the effect of Acrifuran was not observed on the resnlts of total bilirubin in serum, of Takata reaction. of Gros reaction, of Cephaline-Cholesterol test and Bromsulphaleine test. The bad effects on the liver function tests were not only observed with the imposition of Acrifuran, but also the promotion of liver cells' regeneration, and the hypertrophy and the multiplication of ster-cells were observed in the bistological findings of liver.

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Investigation of vicissitude of circulating lymph volume and its bilirubin contents as well as those of serum bilirubin were carried out together with the clarification of the presence of verdohemoglobin, a matrix of biliverdin, by administration of physiological saline or hemoglobin solution via femoral or mesentherial vein on thoracic duct-fistled dogs of the sound, the liver-damaged with the administration of carbon tetrachloride, or the reticuloendothelium-blocked to clarify the characteristic of thoracic duct lymph during the process of bile pigment formation in vivo, and the following results were obtained:
1. It was found that thoracic duct lymph volume always reduced by administration of physiological saline or hemoglobin solution after a temporal variation in the early stage of administration.
2. Temporal increase of bilirubin contents in thoracic duct lymph would often appear even under the administration of physiological saline, and it was apparent in the administration of hemoglobin solution.
3. The increase of bilirubin contents in thoracic duct lymph appeared by turns; the sound>the reticulo-endothelium-blocked>the liver-damaged. It appeared, on the other hand, most dominant by the mesentherial administration on the sound and by the femoral one on the other two.
4. Vicissitude of bilirubin conents in the thoracic duct lymph after the administration of hemoglobin solution was remarkable in the sound, pretty in the reticulo-endothelium-blocked, and scarce in the liver-damaged.
5. Though vicissitude of serum bilirubin seemed to be similarly controlled to the one of thoracic duct lymph, there was not always correlation between the two, and there appeared that at least a part of bilirubin formed in the liver might be transported into the blood flow.
6. No biliverdin could be extracted from the thoracic duct lymph, and the transport of verdohemoglobin from liver to the blood flow, especially this could not be found via thoracic duct lymph.

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Vicissitudes of flowing lymph volume, total protein contents, albumin, globulin, A/G ratio, fibrinogen, and of non-protein nitrogen were followed by tbe administration of allyl formate on the thoracic duct-fistled dogs, and influences of desoxycorticosterone acetate, Acther, cortison, and glucuronic acid on the vicissitude were studied to get the following resutls:
1. Averaged flowing lymph volume on 25 sound dogs at 10 minutes from the thoracic duct-fistle formation was 3.5 cc, total protein nitrogen 511 mg, albumin nitrogen 266 mg/100 cc, globulin nitrogen 171 mg/100 cc, A/G ratio 1.35, fibrinogen nitrogen 33 mg/100 cc, and non-protein nitrogen was 31 mg/100 cc.
2. Administration of allyl formate brought an increase on flowing lymph volume and total protein contents, and the increase was apparent on albumin fraction in the early stage and on globulin fraction in the end stadium. A/G ratio, therefore, increased temporally in the early stage, and afterward the value reduced gradually to reach below that before the administration of allyl formate. Both fibrinogen and non-protein nitrogen increased. These results may suggest that allyl formate brings serous inflammatory process.
3. Administration of Syncorta, a preparate of desoxycorticosterone acetate, brought inhibition of increases of thoracic duct lymph volume and total protein volume, decrease of A/G ratio, and increase of fibrinogen on the allyl fomate-administered dog. It may be concluded that desoxycorticosterone acetate recovers the serous inflammatory process which has been brought by the administration of allyl formate.
4. No definite influence of Acther could be seen on both the sound and the allyl formate-administered with an exception of an increase of thoracic duct lymph volume by a small dosage of Acther.
5. A large dosage of Cortone, a preparate of cortisone, inhibited the serous inflammatory process temporally, but a small one on the contrary brought a remarkable increase of thoracic duct lymph volume.
6. Though temporary, Guronsan, a preparate of glucuronic acid, inhibited the serous inflammatory process induced by the administration of allyl formate.

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Three years ago the patient was pointed out splenomegaly and leukocytosis associated with no swelling of lymphatic glands after pneumonia, but had no complains till admission's time. Leukocytosis was always counted over 10000, and was occupied indifinite large lymphcytes identified as Downey II type cell at 55.6% in maximum of its classification. Bone marrow, red blood cell, reticulocyte and platelets count were normal. Spleenbiopsy showed a full of atypical lymphcytes without leucaemic infiltration in spleen. The infiltration of same atypical lymphcytes with intensely degenerated nature were found by liverbiopsy, and these cells had not any tendency to increase in the liver. Axillary lymphatic gland extirpasion's tissue picture coincided with chronic lymphadenitis, and extensive reticulo- and sinusendotheliar cell increasing were also seen. Several serologic reactions, i.e. Paul-Bunnel reation, Weil-Felix reaction and Davidson's adosorption test had little value to making diagnosis.

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We observed a case believed the peroral infection with the 151 worms of Anchylostoma duodenale. The results are as follow.
1. According to the distribution of Anchylostoma duodenale and Necator americanus, the probability of the both hookworm infections showed almost same percentage in this area. Inspite of the above situation, we observed the case, which could be clearly denied any chances of percutaneous infection, and which was a simple infection of Anchylostoma duodenale. The above facts may show that there are the biological differences on the infection ways of both Anchyolstoma duodenale and Necator americanus and the main infection way of the former is peroral and of the latter is percutaneous.
2. There are many reports on the mixed infection of both Anchyolstoma duodenale and Necator americanus, but almost no cases of Wakana Disease in this area including Southern Kyushu. But the case we observed showed having the possible history of Wakana Disease. We hereby, think the necessity observing more about Wakana Disease epidemiologically.
3. The treatment of raw vesitable juice being highly appriciated at the present time should be reinvestigated for the preventive purpose of not only Ascaris infections but also Anchylostoma infections.

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