I have conducted an experiment, first, by classifying some rabbits into two groups, one, of complete hunger, another, of incomplete hunger, being administered every day such as water, glucose, vitamin B
1, olive oil and water, alcohol, as well as methionine; and then, by using Tiselius' electrophoretic apparat, examined those changes that occur to serum protein fraction, by administering mononutrient, and at the same time examined about the blood changes; by which arrived at the following results:
1. It is methionine that has proved most to prolong the living days; next, alcohol, and then, water.
2. The degree of decline in weight has proved highest in proportion to the length of life time.
3. The number of erythrocytes and haemoglobin quantity has proved about parallel; it has shown some increase under perfect hunger, while, certain decrease or no great change in incomplete hunger.
4, As for the leucocyte number, there were those that have proved a slight increase, or on the contrary, those that have proved certain decrease.
5. As to the total blood nitrogen amount, no great change found in both groups of hunger.
6. The rest blood nitrogen showed a general increase in almost all experimental groups.
7. In every experimental group, the quantity of blood sugar proved either to increase violently before death, or to increase through the whole period of experiment.
8. As to serum protein quantity, it has shown a slight decrease in water given group, a slight increase in methionine-given group, but anyhow no great change found.
9. The serum albumin, whether in perfect of in imperfect hunger, proved somewhat to tend to decrease.
10. Serum α-globulin proved some increase in almost every case.
11. Serum β-globulin, proved certain differences in each group, but no great one.
12. Serum γ-globulin proved to increase remarkably in each experimental group where either methionine, alcohol, or glucose were given.
13. From the electrophoretic observation of serum protein fraction, it has been discovered that for the mononutrient which serves to maintain as well as improves albumin which is principal body protein aud α-globulin which represents antibody protein, glucose stands most efficient; then methionine and alcohol.
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