From Sept. 2 to 13, 1973, acute gastroenteritis with acute diarrhea being its main symptom prevailedamong persons who were revealed to have used a restaurant in Nagoya airport. The number of the patients notified reached 956 in all. Through epidemiological investigations, that outbreak was strongly suggested to be caused by the drinking water from one of two wells used at that restaurant. Bacteriological investigations detected pathogenic E. coli in the feces of 18 patients and the well water, all of which were identified as same serotype pathogenic E. coli (06: K: H16, 027: K: H7, 0148: K: 1128).
In the previous paper, it was described that Pseudomonas aeruginosa was able to consistently multiply in a simple NaCl solution. The present paper is to report the results obtained from the experiment as to whether Pseudomonas aeruginosa could multiply in various simple inorganic salt solutions other than NaCl. 1) It was certainly noted that Pseudomonas aeruginosa could quantitatively multiply in various simple inorganic salt solutions. The degree of multiplication, however, depends upon the kind and concentration of salts which composed the culture solution. 2) There are some strain-dependency of Pseudomonas aeruginosa in the mode of multiplication in the solution tested here, whereas there is no difference in the case of mixtur e of two kinds of inorganic salt, the results of which were previously reported.3) The inorganic salts supporting the multiplication of Pseudomonas aeruginosa are Na2HPO4, MgCl2 and CaCl2. 4) The growth of Pseudomonas aeruginosa in the simple inorganic salt solution can be regarded as intrinsic multiplication, because multiplication of the bacilli in a serial culture was possible in the simple inorganic salt solution just as like as in the mixed inorganic salt solution. From the results described above, it is strongly suggested that the minimum requirement for the growth of Pseudomonas aeruginosa is inconceivably simple.