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Volume 55, Issue 8
Displaying 1-7 of 7 articles from this issue
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Haruo OHNISHI, Hiroshi KOSUZUME, Hitoshi INABA, Shigetoshi SHIMADA, Hi ...1981 Volume 55 Issue 8 Pages 541-550
Published: August 20, 1981
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSInosiplex is a complex of inosine and N, N-dimethylamino-2-propanol-p-acetamidobenzoate (DIP-PACBA) in a molar ratio of 1 3. The activity of Inosiplex and the constituents of Inosiplex, inosine and DIP-PACBA, on the immunocompetence of the host, and against experimentel virus infections, was studied, In addition, the effect of the ratio of inosine to DIP-PACBA on these activities was examined. DIP-PACBA inhibited the growth of herpes simplex virus (HSV), cytomegalovirus, vaccinia virus and influenza virus (INFV) in vitro, but the activity was less potent than that of Inosiplex. Inosine did not inhibit the growth of these viruses. DIP-PACBA increased the survival rate of HSV-infected mice slightly, but the activity was obviously less potent than that of. Inosiplex. No effect of inosine was observed. Inosiplex also strongly inhibited secondary infections due to INFV, while neither inosine nor DIP-PACBA showed such activity. Inosiplex potentiated the function of both lymphocytes and phagocytes. Neither inosine nor DIP-PACBA showed such activity. The most potent immunpotentiating activity was shown for complex of inosine and DIP-PACBA with a: molar ratio of 1: 3. Since the activity of Inosiplex as a potentiator of host defenses was not shown by the individual constituents, inosine and DIP-PACBA, it is suggested that the activity may be exerted by the formation of a complex of the constituents.View full abstractDownload PDF (1191K) -
Haruo OHNISHI, Hiroshi KOSUZUME, Hitoshi INABA, Masatsugu OKURA, Hideo ...1981 Volume 55 Issue 8 Pages 551-557
Published: August 20, 1981
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSThe effects of Inosiplex on viral growth, experimental viral infections and host defense mechaniams were compared with those of Levamisole, Lentinan, PS-K and OK-432. Levamisole slightly inhibited growth of influenza virus (INFV) but its activity was obviously less potent than that of Inosiplex. Lentinan, PS-K and OK-432 did not show any effects on viral growth. Inosiplex inhibited secondary INFV infection. No effect was shown by Levamisole, Lentinan, PS-K and OK-432. This observation appears to reflect Inosiplex's activity as both a viral growth inhibitor and a potentiator of host defense against viral infections. Inosiplex had the ability to restore lymphocyte function which had been depressed by INFV infection in vitro while Levamisole, PS-K and OK-432 had no effect. Lentinan less effectively increased lymphocyte function depressed due to INFV infection in vitro but had no effect on secondary INFV infection, or viral growth in vitro, suggesting that its mechanism of action is different from that of Inosiplex.View full abstractDownload PDF (840K) -
Takashi MIYAKE, Akira FUJIURA, Yuichi ISHIHARA, Hiromasa INOUE1981 Volume 55 Issue 8 Pages 558-565
Published: August 20, 1981
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSRecently, it is noticed that the patients of psittacosis have been increased in number and some deceased cases have been found. Therefore, the check system for this disease is pressingly needed. The antigens for the serological surveys must be prepared personally in our own laboratory, because they have not been on the market.
This report concerns with two studies on Chlamydia psittacosis, the one is a preparation of antigens employing for hemagglutination inhibition (HI) and complement fixation (CF) test, and the other is the serological survey results of the subjects in Aichi prefecture.
1. Preparation of the antigens
HA antigen for HI test was: prepared as follows; Seven to eight day old embryonated eggs were inoculated into the allantoic cavities with 0.2ml/egg of A-8 strain, diluted with saline in 10-2 to 10-4 (originated from human patient, ELD50 10-8.1) and incubated at 35°C for 5 to 8 days. For the CF test, allantoic fluids of 7 to 8 day old eggs which were inoculated with 0.2 ml of the same strain (in dilution of 10-2) into the allantoic cavities and were incubated at 35°C for 6 days, were prepared, and those fluids, revealed the value of 1: 16 to 1: 64 in HA titer, were used as CF antigen.
HA antigen was well preserved for 6 months at-80°C and CF antigen for 3 months at 4°C.
2. Serological surveys
Both of HA and CF tests against psittacosis were surveyed on 180 subjects of the age group of 1 to 40 years old (including the healthy subjects and the ill patients without respiratory infection) in Aichi prefecture. CF positive subjects were observed in 3 of 180 (1.7%) at the titer of≥1: 8 and one of 180 (0.6%) was HI positive at the titer of≥1: 16.
Forty-five paired sera of the patients with respiratory diseases or febrile diseases were also examined, one subject among febrile patients showed a significant increase of CF antibody.View full abstractDownload PDF (930K) -
Keizo YAMAGUCHI, Akihiro HAMAMOTO, Yoshito TANAKA, Yoji SUZUYAMA, Hisa ...1981 Volume 55 Issue 8 Pages 566-575
Published: August 20, 1981
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSPresent paper discussed on the results of both clinical and laboratory findings of patients with typhoid fever, who were admitted to Nagasaki Municipal Medical Center during the period from 1963 to 1980.
Number of patients was 2 to 12 cases in each year and a total of 95 cases were objected in this study.
The cases with the typical symptoms of typhoid fever were very few because of early chemotherapies, and some of them were transfered to our hospital as FUO or under the diagnosis of influnza, blood disease, hepatitis, rheumatic fever and so on.
Regarding to laboratory findings on admission, positive CRP and elevations of cc2-globulin, LDH values which reflect inflammation were found in the majority of the patients and a positive rate for RA test was 37.5 percent. LDH isozymes showed increases of fraction 4 and 5 and relative decreases of 1 and 2. Furthermore, slight to moderate elevations of GOT, GPT, γ-GTP, alkaline phosphatase and LAP were frequently observed, but almost all of these abnormal findings were rapidly improved on convalescent period after the chemotherapies.
Isolation rate of S. typhi from the patients was highest from the bone marrows, followed by blood, biles, feces and urine in order.
Susceptibilities of 27 clinical isolates of S. typhi to 16 kinds of antibiotics were tested by the agar dilution method. The result revealed that Cefotaxime was most excellent regarding to antibacterial activity and its MIC value was 0.05μg/ml with inoculaum size of 106 cells/mi. Pivmecillinam followed it with MIC value of 0.05 to 0.1μg/ml and these of CP, A BPC and ST which had been often used clinically were 3.13, 0.39 to 0.78μg/ml, respectively.View full abstractDownload PDF (1191K) -
1. Coagulase types and crystal violet ring test of isolates from clinical specimenKiichi GOTO1981 Volume 55 Issue 8 Pages 576-582
Published: August 20, 1981
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSCoagulase typing and crystal violet ring (CVR) test for Staphylococcus aureus isolated from clinical specimen were carried out and following results were obtained.
Coagulase types of isolates from the throat swabs of infants with acute pneumonia and bronchitis were distributed into the coagulase type I-VIII Staphylococci isolated from the sputum from suspected adult cases of tuberclosis had the distribution of the same coagulase types.
Although, the organisms with coagulase type IV were detected dominantly in the pus from festering wound and abscess, the coagulase type V cocci were isolated frequently in the purulences from otitis.
The correlation between the coagulase types and CVR test patterns were discussed. As the result, the high incidence of WR group (a faint violet ring) was observed in the coagulase type II cocci.View full abstractDownload PDF (846K) -
2. A long-term observation of Staphylococcus aureus isolated from mouth washings of healthy adultsKiichi GOTO1981 Volume 55 Issue 8 Pages 583-591
Published: August 20, 1981
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSA long-term observation on coagulase, enterotoxin type and crystal violet ring test pattern of Staphylococcus aureus was carried out on mouth washings of 32 healthy adults served in a public agencies.
The positive isolation of staphylococci was 60.3%, and 169 out of 202 (83.7%) isolates were the organisms belonged to the coagulase type II, E, VI or VII. Although there was no change in the coagulase types of the isolates from the most persistent healthy carriers, that of isolates from the 6 healthy adults changed transiently.
The highest antibody titers against corresponding coagulase types of the strains isolated were found in the sera of persistent carriers. Sera of healthy adults from which no organisms were detected contained 2-mercaptoethanol sensitive antibody.
It seems likely that staphylococci in upper respiratory tract are able to contaminate food and environment.View full abstractDownload PDF (1147K) -
1981 Volume 55 Issue 8 Pages 592-594
Published: August 20, 1981
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSDownload PDF (379K)
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