Ozone sterilization was applied to a bioclean room and its effect was compared with that of formaldehyde sterilization, which has been commonly used.
When the sterilization effect of ozone was examined by using E. coli, B. subtilis and Penicillium sp., B. subtilis had the most resistance to ozone and remained alive for 72 hr, on the other hand, Penicillium sp. immediately lost its activity. It was, therefore, considered that the 72-hr value of B. subtilis was repuired as an index value for the evaluation of the effect of ozone sterilization.
When the statistical evaluation was done as to the effect of ozone sterilization, temperaturedependence was not seen, but the twice sterilization was much more effective than the once one. Also, the effect of ozone sterilization was statistically comparable to that of formaldehyde.
From those results and some merits of ozone; ex. gas generation is easy, no adhesion to materials occures and exhausting time is short, while formaldehyde has some demerits like paraformaldehyde formation and so on, ozone sterilization method seems to be practically superior to the usual method of formaldehyde sterilization.
When the statistical evaluation was done as to the effect of ozone sterilization, temperature dependence was not seen, but the twice sterilization was much more effective than the once one. Also, the effect of ozone sterilization was statistically comparable to that of formaldehyde.
From those results and some merits of ozone; ex. gas generation is easy, no adhesion to materials occures and exhausting time is short, while formaldehyde has some demerits like paraformaldehyde formation and so on, ozone sterilization method seems to be practically superior to the usual method of formaldehyde sterilization.
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