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Volume 56, Issue 8
Displaying 1-9 of 9 articles from this issue
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Hirofumi OHASHI1982 Volume 56 Issue 8 Pages 647-654
Published: August 20, 1982
Released on J-STAGE: November 25, 2011
JOURNAL FREE ACCESSThis study was done to assess the variations of vaginal microbial flora according to pregnancy and vaginitis. The normal adult flora was compared with that of women in pregnancy and of women with vaginitis.
The subjects of the study were 218 non-pregnant healthy women, 219 pregnant women and 119 women with vaginitis who visited the Department of Obstetrics and Gynecology at the Central Hospital of Japanese National Railways between October 1977 and September 1981. By discharge samples obtained from the vaginal lateral wall, aerobe, anaerobe and fungi were cultured and isolated.
Lactobacillus, Candida albicans and Propionibacterium were isolated significantly higher from pregnant women than from non-pregnant women. But E. coli, Peptococcus and Bacteroides were isolated significantly lower from pregnant women than from non-pregnant women. The isolation rate of Lactobacillus was significantly lower and that of Candida albicans was significantly higher from women with vaginitis than from women without vaginitis.
No statistically significant differences have been shown in the isolation rate of microorganism during the stage of pregnancy.
The isolation rate of Lactobacillus tended to be lower and that of Peptococcus, Peptostreptococcus and Bacteroides tended to be higher from women with non-specific vaginitis than from women with candida vaginitis.
No significant differences of isolation rate of microorganism between non-pregnant healthy women and women with candida vaginitis. Lactobacilli were isolated significantly lower and Peptococcus, Peptostreptococcus, Veillonella and Bacteroides, significantly higher from women with nonspecific vaginitis than from non-pregnant healthy women.
The conclusion from the above study was that variations of microbial flora according to pregnancy and vaginitis (especially non-specific vaginitis) were recognized.View full abstractDownload PDF (976K) -
Nobuyoshi TACHIBANA, Eiji KUSUNE, Tsutomu YOKOTA, Eiichi SHISHIME, Kaz ...1982 Volume 56 Issue 8 Pages 655-663
Published: August 20, 1982
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSSince 1979 number of patients with tsutsugamushi disease began to increase in Miyazaki district, and 41 cases were recognized in 1980. Immunologic, etiologic and epidemiologic study were carried out, and following results were obtained.
1) Patients occurred from late October till middle December, mostly between middle November and early December. Geographically one half of the patients were from Nojiri-cho, and the remaining were from adjacent area. In these area no tsutsugamushi disease was recognized in the past.
2) Immunofluorescent antibody titers of patients' sera against Gilliam, Karp, Kato, Irie as the representative strain in Miyazaki, and Hirano strain newly isolated from a patient, were tested. Twenty-eight of 40 serum samples showed the highest titer against Irie antigen, and 10 against Hirano antigen, showing that selection of antigen is important in serological study in tsutsugamushi disease.
3) R. tsutsugamushi were isolated from 19 patients. All strains were low virulent to mice. Immunological characteristics were examamined by cross immunofluorescent antibody test among 11 isolated strains. The result showed that 5 strains were similar to Gilliam strain as Irie, and 6 strains resembled to Karp strain.
4) Infected BSC-1 cell was very useful in preparing antigen for immunofluorescent antibody test in low virulent strain of R. tsutsugamushi.View full abstractDownload PDF (998K) -
Nobuyoshi TACHIBANA, Eiji KUSUNE, Tsutomu YOKATA, Eiichi SHISHIME, Kaz ...1982 Volume 56 Issue 8 Pages 664-670
Published: August 20, 1982
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSSince the strains of Rickettsia tsutsugamushi isolated from patients in Miyazaki district are very low virulent to mice, to yolk sac of embryonated hen eggs, and to cultured cells, it is a major problem in studies of the disease to obtain growth systems for the agent.
It was show that the rickettsiae of these low virulent strains abundantly grow in African green monkey kidney (BSC-1) cell. Infected cells did not show remarkable changes in cell appearence under microscopic examination, unlike virulent strains such as Gilliam, Karp and Kato, but degenerated 15 to 30 days after inoculation of the rickettsia when cytoplasm of cells filled with rickettsial particles.
Fifty per cents infective titers of cells infected with Irie, the representative strain in Miyazaki district, were 10-7.5 in BSC-1 cell and 10-7.2 in mice respectively, indicating high susceptibility of the cell to the rickettsia as comparable to cyclophosphamide treated mice.
In an attempt for isolation of the rickettsia from patients' blood with BSC-1 cell, three of the 6 samples showed positive results by the cell and 5 of the same samples by mice, suggesting availability of the cell in this purpose.View full abstractDownload PDF (4299K) -
Kazuhito KAMIYA, Hisayoshi SUGIHARA, Tetsunosuke TANAKA1982 Volume 56 Issue 8 Pages 671-678
Published: August 20, 1982
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSExperiments were performed in mice to assess the protective effect against S. enteritidis infection of SPA purified from S. enteritidis strain 2547. Single injection of purified SPA, 0.05μg i.p., provided protection in 80% of mice against death from infection by challenge with 104 LD50 of the organism 14 days after injection (previous report).
When normal mice were challenged with 100 LD50 of the organism after passive transfer of mouse anti-serum to purified SPA, 90% of the mice survived, whereas all normal controls receiving passive transfer of normal mouse serum died following the challenge. Bacterial enumeration in these animals showed growth of the pathogen to 105 organism/ml in the blood and 108/g in the spleen and liver of the controls at 3 days after infection. The pathogen did not multiply to reach such high blood and visceral levels and gradually diminished in number to become completely eliminated in 30 days after challenge in the immune serum injected group.
There was little or no bactericidal effect of the immune serum, but a significant increase of bactericidal rate was evident as compared to controls when the immune serum was combined with normal macrophages and incubated with the challenging pathogen, followed by enumeration of viable bacteria.
Normal mice given the immune serum by passive transfer after intraperitoneal injection of carrageenan for suppression of macrophage function, all died following a subsequent change with the pathogen.
The data obtained have demonstrated the IgM was the component taking a principal part in this protection against S. enteritidis infection, among other antibodies contained in the immune serum.View full abstractDownload PDF (979K) -
Jun IGARI, Nozomu KOSAKAI, Toyoko OGURI1982 Volume 56 Issue 8 Pages 679-684
Published: August 20, 1982
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSThis paper describes the clinical and bacteriological features of three patients with bacteremia due to Aeromenas species during one year period at Juntendo University Hospital in Tokyo.
All of the patients were hospital-aquired and had past histories of a major abdominal surgical operation. Two patients has a gasterctomy for gastric carcinoma and one patient had a cystectomy for a cyst of common bile duct and a cholecystectomy for chronic cholecystitis. The patients, who had a gasterectomy, were in their teminal stage of malignancy and had impaired host defens secondary to neoplasia.
The isolated strains from three patients were sensitive to Doxycycline, Minocycline, Gentamicin and Chloramphenicol and resistant against Ampicillin, Sulbenicillin and Cephazolin.
Aeromanas species is a cause of nosocomial bacteremia in compromised hosts.View full abstractDownload PDF (769K) -
A Multi-center Study with the Same ProtocolShunkichi BABA, Tsutomu HATANO, Kanetaka MURAI, Takeshi MARUO, Kazuko ...1982 Volume 56 Issue 8 Pages 685-704
Published: August 20, 1982
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSClindamycin-2-phosphate is an injectable preparation of clindamycin which has a high antibacterial activity against gram-positive cocci and anaerobic bacteria. The purpose of the present study was to evaluate the effect of the drug in the clinical treatment of various otorhinolaryngological infection.
Fourty-seven out of 64 patients with suppurative otitis media were treated successfully, i.e., evaluated as good or excellent (efficacy rate was 73.4%). Similarly, 15 out of 26 patients with paranasal sinusitis (57.7%), all nine patients with peritonsillar abscess (100%), 14 out of 15 patients with acute tonsillitis (93.3%) and 7 out of 8 patients with other diseases such as acute pharyngitis, etc. (87.5%), were successfully treated.
Side effets were observed in one patient with pain at injection site, one with eruption with feeling of pruritus, and one with eruption (incidence of side effect was 2.5%; 4/158). None ofthe side effects were serious; all of them cleared in 2 to 4 days. Among abnormal laboratory findings observed were an increase in GOT and GPT, urinary protein, an increase in BUN, and leukocytosis; none of them were particularly serious.View full abstractDownload PDF (2146K) -
Shinichi FUJITA, Fujitsugu MATSUBARA, Yatsugu NODA1982 Volume 56 Issue 8 Pages 705-710
Published: August 20, 1982
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSA case of infective endocarditis due to a nutritionally variant streptococcus is described.
The patient, a 45-year-old male was admitted to our hospital because of a fever. All the five blood cultures performed during the first 10 days of hospitalization were positive for Streptococcus. This strain could not be subcultured on blood agar media used routinely in our laboratory. Successful subculture of this isolate was achieved, however, as a satellite growth surrounding a variety of organisms. The minimum inhibitory and bactericidal concentrations of penicillin G for the blood isolate were 0.025μg/ml and 6.25μg/ml respectively. The highest dilution of serum with bactericidal activity against the isolate during intravenous instillation of 20 million units of penicillin G over a 24-hour period was 1: 32, as tested in a supplemented Todd-Hewitt broth.
Penicillin G treatment resulted in successful convalescence of the present case of infective endocarditis. Our experience gives warning to clinicians as well as microbiologists against the possibility of endocarditis due to nutritionally variant streptococcus which can not be subcultured on certain routine agar media.View full abstractDownload PDF (626K) -
Mitsunobu AKASHI, Hiroyasu ARATANI, Akihiro HAMAMOTO, Hisashi ISHIKAWA ...1982 Volume 56 Issue 8 Pages 711-714
Published: August 20, 1982
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSThe patient was a 34 year-old man returned to Japan on the 7th of October, 1980 after 3 days stay in the Philippines. After that his mushry stool of two to three times a day had persisted.
In April 1981, his son complained high fever and watery stool. He was also attacked with high fever and abdominal pain one month later.
Four gas-producing Shigella boydii 14 straiCase of dysentery caused by this kind of strain is the first report in Japan.zns were isolated from stool of him and his family, and strains had resistant to TC and SM.
Case of dysentery caused by this kind of strain is the first report in Japan.View full abstractDownload PDF (410K) -
Yoshio AOKI, Setsuko TAZAWA, Yoshiko NAKAMURA, Hiromitsu MATSUMOTO, Ak ...1982 Volume 56 Issue 8 Pages 715-723
Published: August 20, 1982
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSGram stain indeterminate diplococci were isolated from five consecutive blood cultures of a septic patient with valvular disease of the heart. These strains (SK) were identified to be Gemella haemolysans by bacteriological and immunological examinations.
The isolated cocci occurs singly or in pairs with adjacent sides flattened, or sometimes in tetrad under the microscope. There is considerable variation in size. It grows very small smooth colonies on blood agar at 37°C for 24 hours, and shows very feeble growth on tryptosoy agar and β-hemolysis on rabbit blood agar.
The biochemical properties of this organism are as follows. Catalase is negative. Cytochrome oxidase is negative. Acid is produced without gas fermentatively from glucose, fructose, maltose, and sucrose, while lactose, mannitol, trehalose, xylose, melibiose, and raffinose are not fermented. There is no reduction of nitrates and VP test is negative. Type strain (ATCC 10379) shows the identical biochemical and biological properties as those of SK strain. This organism is sensitive to penicillins, cephalosporins, kanamycin, chloramphenicol, tetracycline, lincomycin, and erythromycin.
The patient's sera were obtained in convalescent stages three times at intervals of one week. These sera reacted to SK strain and ATCC 10379 at a concentration of 102 to 104 dilution as indicated by indirect immuofluorescent antibody technique using fluoresceinated rabbit anti human antibody.
Gemella haemolysans is thought to be an indigenous bacteria in bronchial secretions and in slime from the respiratory tract of mammals. With regard to the pathogenesis of this organism, there may be no information up to date. So it is considered that this report is the first case of endocarditis due to Gemella haemolysans.View full abstractDownload PDF (3062K)
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