The recognized biological role of cyclic AMP has grown from a relatively simple role in liver glycogenolysis to the point where it appears to be a key regulatory agent in most mammalian tissues.
The present investigation was conducted to explore the effect of various vasoactive substances on the cyclic nucoleotide contents of guinea-pig lung and tracheal tissues.
Male guinea-pigs, weighing 250-300g, were used. The cyclic nucleotide contents of guinea-pig lung and tracheal tissues were determined by radioimmunoassay method.
1) The cyclic nucleotide contents of guinea-pig lung and tracheal tissues reached a maximum in about 15min. by incubating with aminophylline (1mM), decreased and reached a plateau in about 30min..
2) The cyclic AMP content of guinea-pig tracheal tissues reached a maximum in 2min. by incubating with acetylcholine, isoproterenol, prostaglandin E
2 (PGE
2) and histamine, reached a maximum in 3min. by incubating with PGF
2α.
3) The cyclic AMP content of guinea-pig lung tissues reached a maximum in 2min. by incubating with isoproterenol, PGE
2 and histamine, reached a maximum in 3min. by incubating with PGF
2α.
4) The cyclic GMP content of guinea-pig tracheal tissues reached a maximum in 2min. by incubating with acetylcholine and histamine, and did not show any change by incubating with isoproterenol and PGE
2.
5) The cyclic GMP contents of guinea-pig lung tissues reached a maximum in 2min. by incubating with PGF
2α and histamine, reached a maximum in 1min. by incubating with acetylcholine and PGE
2, and did not show any change by incubating with isoproterenol.
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