The investigations on bacteriocin (marcescin) typing of 554 Serratis marcescens strains isolated from clinical materials during the period from April, 1970 to April, 1976 in Nagasaki University hospital were carried out by agar streak method (D.S.T. agar (Osoid) used), and the results were as follows.
1. The method was used with complete checker-board experiment based on bacteriocin productive and sensitive reaction. Out of 80 Serratia marcescens strains, 43 (53.8%) were productive, 74 (92.5%) were sensitive and 4 (5.0%) had not these character. Immunity was confirmed in all strains.
2. From 80 Serratia marcescens strains, 8 strains were selected as the indicator strains in the point of differentiation. By using these indicator strains bacteriocin typing was made in 554 isolated strains.
In bacteriocin production typing, 554 strains were classified into 26 types including one type of bacteriocin non-productive strain. In bacteriocin sensitivity typing, 554 strains were classified into 42 types including one type of resistant strains. Two hundred and ninety six strains in which no bacteriocin activity was observed against 8 indicators were classified into 31 types by bacteriocin sensitivity typing, and 64 strains in which were not sensitive to bacteriocin were classified into 7 types by its production typing. Twenty nine strains (5.2%) were not classified by both methods.
3. Bacteriocins produced by this method were active to family enterobacteriaceas, and Aeromonas hydrophila, one of family virionaceae and were not active to 121 glucose non-fermentative rods. From the results of resistance to chloroform, heat and trypsin, it was considered that these were belonged to high molecular bacteriocin, marcescin group A reported by Prinsloo (1966).
4. In the study of reproducibility in bacteriocin typing, it was confirmed that the types of some strains were unstable, but even in those strains same results were obtained in 2 experiments out of 3 repeated ones. Bacteriocin production typing was more stable than its sensitivity one.
5. In comparison with bacteriocin production typing and serotyping (0 antigen), bacteriocin typing was better than serotyping in classifying of this organism, and 100 Serratia marcescens strains untypable by serotyping were classified into 13 types by bacteriocin typing.

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A 67-year-old female patient with Klebsiella liver abscess developing septic shock with DIC is described.
Following antecedent low-grade illness, high spikng fever and cloudness of consciousness were noted in November 17, 1975. On examination she was dehydrated and drowsy. No jaundice and hepatomegaly. Laboratory findings include ESR 85mm/hr, WBC 24, 000, Pl. 56, 000, total bilirubin 9.3mg/ 100ml with Al-p 7.6 B-L., SGOT 150, SGPT 100, LDH 960. Blood coagulation studies could not be done because of hypercoagulability of the blood. FDP 10μg/ml (normal<10μg/ml). Blood culture yield Klebsiella pneumoniae.
In November 23, she developed septic shock suddenly. Marked generalized bleeding tendency, jaundice and left hemiparesis were noted in November 24, 25, and 26 respectively. She expired in November 27.
In autopsy, solitary liver abscess, cerebral hemorrhage, Klebsiells meningitis were found. Multiple pulmonary, splenic and uterine infarcts were also noted.
This case we present here showed an unusual clinical picture resulting in septic shock associated with DIC.

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