All issues
Volume 79, Issue 2
Displaying 1-8 of 8 articles from this issue
- |<
- <
- 1
- >
- >|
-
Yoshihiro KOBASHI, Kouichiro YOSHIDA, Naoyuki MIYASHITA, Yoshihito NIK ...2005 Volume 79 Issue 2 Pages 111-116
Published: February 20, 2005
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSA clinical analysis of four patients diagnosed as pulmonary actinomycosis in our respiratory division during the last seven years was performed. All of the patients were males with an average age of 61 years. Three patients had a past history. The clinical diagnosis on admission was lung cancer or pulmonary suppuration in three patients showing a mass-like shadow and pneumonia in one patient with an infiltration shadow. Suspected pulmonary infectious disease was detected from clinical symptoms in two patients, while suspected lung cancer was detected in the other two patients during health examinations. The lesions dominantly existed in the right upper lobe. Such findings as central low attenuation (LAA), bronchiectasis and pleural thickening were characteristic on chest computed tomography (CT). The diagnostic methods were all negative procedures; video-assisted thoracoscopic surgery (VATS) in two patients, a specimen obtained by bronchoscopy in one and a specimen taken by percutaneous aspiration in one. Because penicillin antibiotics were administered for a long time for all patients after obtaining a definite diagnosis, the prognosis was good and there were no relapse.View full abstractDownload PDF (2578K) -
Comparison with Virus Isolation and RT-PCR Amplifying Viral Protein 1 RegionKenji YAMAZAKI, Toru OTAKE2005 Volume 79 Issue 2 Pages 117-121
Published: February 20, 2005
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSDetection of viral genomes with RT-PCR, which amplifies viral protein 1 region, as well as virus isolation was performed on 860 patients (996 specimens) who had been suspected for enterovirus (EV) infection in Osaka Prefecture from April 2003 to Jury 2004. The viral positive rates of the clinical materials, combining above two procedures, were as follows: 48.2% from the feces, 38.3% from the throat swabs, and 18.0% from the cerebrospinal fluids. The positive rate by the clinical diagnosis indicated that herpangina was the highest at 44.7%, while encephalitis was the lowest at 13.4%. Out of the all specimens, the viral positive rate of RT-PCR varied from 22.3 to 24.7%, while that of virus isolation was 15.6%. The total viral positive rate of both procedures combined was 29.8%. Since the detection of EV by RT-PCR amplifing viral protein 1 region was more rapid than virus isolation and superior in detection of coxsackie group A virus, RT-PCR is an effective procedure for diagnosis of herpangina.View full abstractDownload PDF (655K) -
Noritoshi KITAMOTO, Yoji KATO, Kanako HAMADA, Shihono KANZYA, Takashi ...2005 Volume 79 Issue 2 Pages 122-128
Published: February 20, 2005
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSDuring the years 1983 to 1999, a total of 120 Salmonella Enteritidis (SE) isolates from various sources, patient's stool, foods, kitchen wear, river water etc., in 61 cases of food poisoning in the Sakai City, were typed by pulsed-field gel electrophoresis (PFGE) afterXbaIorNotIdigestion of chromosomal DNA.XbaIandNotIrestriction produced 2 (X1 and X2) and 3 (Ni, N2 and N3) pulse-field profiles, respectively. The X1 and Ni types were further divided into 8 (X1a-X1h) and 6 (N1a-N1f) subtypes, respectively. However, these strains of subtypes showed only 0-4 fragment changes in PFGE patterns and the index of discrimination of over 0.75, indicating that SE isolates belong to the same clonal lineage, or are revealing closely clonal relationships. These results suggested a possible strain transmission in case of food poisoning, and epidemiologically related SE isolates were spread in the Sakai City district during a long period.View full abstractDownload PDF (2213K) -
Noritoshi KITAMOTO, Yoji KATO, Shihono KANZYA, Atsuo KATAI, Tomoyuki T ...2005 Volume 79 Issue 2 Pages 129-137
Published: February 20, 2005
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSA total of 103 methicillin-resistantStaphylococcus aureus(MRSA) isolates from patients and 78 MRSA from hospital workers during the years 1990 to 1994, and 52 MRSA from patients in year 2000, in one hospital at Wakayama Prefecture, were typed by pulsed-field gel electrophoresis (PFGE) profiles. MRSA isolates were grouped into 20 genomic types. These types were further divided into subtypes. The type 1 was dominant among patients, and closely related type 1 strains were spread during 1990-1993. In contrast, the type 2 was dominant among medical workers in the same period. In 2000, the type 2 and 4 strains increased, and the diversity and complication of types appeared. The common types between the patients and medical workers were only type 1 to 3. Some strains isolated from both during the same period were shown to have the same types. Others were shown to be patient-specific types. These results suggest that there are complicated transmissions of MRSA in the hospital, i.e., the endogenous infection, patient-patient cross infection and medical worker-patient cross infection.View full abstractDownload PDF (2450K) -
Matsuo DEGUCHI, Masanori KAGITA, Seishi ASARI, Yoshinori IWATANI, Taka ...2005 Volume 79 Issue 2 Pages 138-142
Published: February 20, 2005
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSMutants of hepatitis B virus surface antigen (HBsAg) are known as a cause of false-negative results in diagnostic tests for HBsAg; particularly when a diagnostic kit utilizes monoclonal antibodies to detect HBsAg. We compared seven HBsAg kits with regard to sensitivity for HBsAg subtypes (ad, ay) and their ability to detect nine different HBsAg mutants. Among them, the sensitivities of five kits were high and comparable to each other (0.2-0.3ng/m1). However, two kits were of lower sensitivity (0.8-1.3neml, and 2.4-2.5ng/ml, respectively). Two kits, produced by the same company, reacted with all of the nine HBsAg mutants, but five kits showed false-negative results with one or more of the HBsAg mutants. These data indicate that there are differences in the detection sensitivities for HBsAg and abilities to detect HBsAg mutants among commercially available HBsAg kits, which may explain false-negative clinical results.View full abstractDownload PDF (811K) -
Tomokazu SUZUKI, Akifumi IMAMURA2005 Volume 79 Issue 2 Pages 143-148
Published: February 20, 2005
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSChronic mucocutaneous candidasis (CMC) is a chronic intractable infection of skin, nails, and mucous membrane withCandida. Until very recently, the main stay of therapy had been the use of transfer factor or antifungal azole derivatives. Although they show definite benefits, the effects are temporal and recurrences are inevitable. Furthermore, the prolonged use of antifungals will sometimes induce resistant strains, making the treatment more difficult. Recently we experienced a case of CMC caused by resistantCandidaspp. and treated it successfully with a new antifungal agent, micafungin (MCFG).
The patient is a 37-year-old woman. She was eight month, her tongue was covered with a white coat. Two months later, intractable cutaneous eruptions appeared on the head and back and the diagnosis of CMC was made. Since then she has been treated on multiple occasions with transfer factor, recombinant IL-2, ketoconazole or clotrimazole. She was referred to us because of esophageal candidiasis. On admission, oral and esophageal mucous membranes were thickly coated with white pseudomembranes. The titer of Candida antigen test was less than twice;plasma β-D-gulcan was 20.14 pg/mL;and CD4 was 376/μL. A fewCandida albicansand (1+)Candida glabratawere cultured from oral swab.Both species were resistant to itraeanazale but sensitive to MCFG and amphoteriein B (MIC: < 0.03μg/ml for both).A drip infusion of MCFG (75mg/day) was started and three days later the orallesions disappeared. At the end of a 2-week course of i.v. MCFG, the interior of the esophagus was clear. No recurrence was noted in one month. Less toxic than amphotericin B, MCFG will be a drug of choice in patients infected with azole-resistant fungi. To avoid the abuse of MCFG and the development of the resistant strains, the susceptibility test is recommended in every case of systemic candidiasis.View full abstractDownload PDF (2070K) -
Toru SHIZUMA2005 Volume 79 Issue 2 Pages 149-152
Published: February 20, 2005
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSA 28-year-old male was admitted to our hospital with tonsillitis and jaundice. Laboratory findings showed leukocytosis (rate of atypical lymphocytes was 40%), liver dysfunction and hyperbilirubinemia. Epstein-Barr virus (EBV) viral capsid antigen (VCA) IgM and IgG antibodies were positive, and EB nuclear antigen (EBNA) antibody was negative. Abdominal ultrasonography demonstrated hepato-splenomegaly and swelling of intraperitoneal lymph nodes. A diagnosis of infectious mononucleosis was made due to EBV infection. Conservative therapy was given. Total bilirubin and alkaline phosphatase increased to maximum levels of 10.2 mg/dl and 1, 590U/l. A liver biopsy specimen revealed infiltration of lymphocytes in sinusoids and portal areas, focal necrosis and intrahepatic cholestasis in parenchyma. Liver function tests returned to normal limits and EBV VCA IgM antibody became negative within 10 weeks from onset.View full abstractDownload PDF (2596K) -
Yan CAI, Motohiko OGAWA, Agus SETIYONO, Hideto FUKUSHI, Kenji TABARA, ...2005 Volume 79 Issue 2 Pages 153-154
Published: February 20, 2005
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSDownload PDF (341K)
- |<
- <
- 1
- >
- >|