1) Salivery types in 78 mongrel dogs were divided into two groups, namely Ótype of 56 dogs (71.8%) and A' type of 22 dogs (28.2%).
2) Crossmathches were done in use of dog's plasma, and the agglutinations reacted more strongly than in use of sera. The number of positive reactions was 362 in 1694 trials (21.3%) and was 304 in 847 combination groups (35.8%). Anti Aagglutinin was demonstrated in the plasma of some O'typed dogs.
3) Suspension of donor's erythrocytes was injected repeatedly in recipients, and consequently the rise of antibody value was observed in 1 of 7 combination groups. The antibody might be weak anti-A.
4) Micrpfilariae were detected in the peripheral blood of dogs in rate of 27.5%.
5) Mongrel dogs were divided into 2 groups by the crossmatching and the detection of microfilariae. Crosstransfusion and extracorporeal circulation were done, and the agglutinationreactions and infections of filariae were apparently concerned with bloodtransusionreaction in dogs.
6) The bloodtransfusionreaction could be largely prevented by the preliminary examinations of salivery type, crossmatching and detection of microfilare.
7) Investigation of the openheart surgery in dogs were indispensable to the progress of clinical heart surgery. Up to the present, the effects of massive bloodtransfusion in dogs were apt to be neglected. Therfore, author tried to investigate the cffects of the massive bloodtransfusion in dogs were apt to be neglected. Therfore, author tried to investigate the the effects of the massive bloodtransfusion and deviced the simplc and effecfive method of their protection.
8) The causes of so-ccalled bloodtransfusionreaction in dogs were discussed.

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1. Experimental Studies
Nineteen mongrel dogs were used to study the arterial pressure, adrenal venous blood flow, concentration of free 17-OHCS in adrenal venous plasma, secretion of free 17-OHCS in adrenal venous plasma and concentration of free 17-OHCS in peripheral venous plasma in the purpose of exploring the variation of adrenocortical function experimentally.
1) Average of adrenal venous blood flow is 6.56 cc/min, and that of 17-OHCS concentration in adrenal-venous-plasma is 197.1 μg/dl before extracorporeal circulation. Secretion of 17-OHCS is 6.25μg/min and the concenraition in peripheral venous plasma is 12.2μg/dl.
2) Relationship between arterial blood pressure and adrenal venous blood flow in different kinds of extracorporeal circulation is parallel, when they are observed in normo-and hypothermal perfusion groups.
3) Adrenal venous blood flow in normothermal group is inversely proportional to 17-OHCS concentration in adrenal venous plasma, but not in hypothermal group.
4) As to secretion of 17-OHCS in adrenal venous plasma, the normothermal group shows the increase of secretion, while hypothermal group shows the decrease which seems to be resulted from the functional inhibition of adrenal cortex.
5) Secretion of adrenal gland is directly proportional to 17-OHCS concentration in the peripheral venous plsma.
6) Adrenal tissue does not show any remarkable histological changes after perfusion.
2.Clinical Studies
Adrenocortical functions of 13 clinical cases in normo-and hypothermal extracorporeal circulation were investigated by estimating of free-17-OHCS concentration in peripheral venous plasma and excretion of 17-OHCS and 17-KS in urine.
1) Arterial blood pressure in cold perfusion under 35°C sustains almost constancy, 60mm Hg., while that in warm perfusion shows higher values.
2) Though 17-OHCS concentration in plasma during the perfusion decreases markedly in hypothermal group and increase again by warming, hypothermal group shows nothing changeable. Both normo-and hypothermal group show the same postoperative variation curve. Concentration becomes highest 3-6 hours after the operation, then it gradually decrease, and comes back to normal value after 5 days.
3) No remarkable difference can be found in 17-OHCS concentration in plasma between Z-uhdi and Kay-cross typed artificial oxygenator.
4) Excretion of urinary 17-OHCS has an almost parallel relationship to the postoperative variation of 17 OHCS concentration in plasma. The excretion becomes maxmal in the following day of operation and recovers to normal on the fifth day.
5) When excretion of urinary 17-OHCS is observed from viewpoint of perfused volume, low perfused group shows higher excretory value than that in high perfused group.
We could not obtain any experimental and clinical evaluation to suggest the adrenal insufficiency.

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Metabolism of iron sorbitol and iron dextaran was examied clinically and experimentally by Fe⁵⁹-labeled preparation.
The following results were obtained.
1) Circulating blood volume determined with Fe⁵⁹-labeled iron dextran preparation coincided with that obtained by dye method.
2) Radioactivity disappeared exponentially from peripheral blood initial several hours after single intravenous injection of Fe⁵⁹-labeled iron dextran containing 90 mg of iron (specific activity 4.4 μc/30mg) both in clincal cases and in experimental rabbits. Plasma iron disappearance T/2 in clinical cases was 7-9 hours and 50-140 minutes in experimental rabbits. Thereafter, the radioactivity disappeaed at slower rate.
There was no significant difference of PID between the anemic and the normal both in patints and raddits.
100% of Fe⁵⁹ injected was utilized for Hb synthesis 13 to 25 days after single intravenous injection of the preparation in patients with iron deficiency anemia, while in nomal subjects utilization at 25 th day after injecion was around 80%. Similer results were obtained in experimental rabbits.
3) A marked reduction in UIBC was observed during the course of treatment with iron dextran. Although no significant change was found in UIBC following single intraveous injection of 50mg of iron as iron dextran.
4) Radioactivity was deposited mainly in the liver and bone marrow after single intravenous injection with Fe⁵⁹-labeled iron dextran in experimetal rabbits, while a larger amount of Fe⁵⁹ was deposited in the site of the injection after single intramuscular administration.
In the latter case, high radioactivity was detected in the regional lymph nodes of the injection site. From these results, it was suggested that intramuscularly injeted iron dextran was absorbed via lymphatic system.
5) Radioactivity appeared in peripheral blood rather rapidly following single intramuscular of Fe⁵⁹-labeled iron sorbitol preparation, containing 90mg of iron (3.36μc/50mg/ml), and its maximal level was attained by 3-6 hours after the injecton in patients with iron deficiency anemia. Virtually no radioactivity was left in plasma 24 hours after the injection.
About 30% of Fe⁵⁹ was excreted to urine.
Utilization of Fe⁵⁹ by red blood cell was rapid as compared with the case of iron dextran, and plateau levels of around 70% was attained by 8-10 th day following single injection of Fe⁵⁹-labelcd iron sorbitol.
6) More than 30% of the iron was excreted to urine during the course of treatment of iron deficiency anemia with intramuscular injection of iron sorbitol (daily dose of iron injected 100mg).
It was noted that color of urine become dark as amount of the preparation excreted was increased.
Slight but evident toxic effect developed following intramusclar injection of 200 mg of iron as iron sorbitol. Allergic eruption was observed in a patient with iron deficiency anemia. Increase in Hb levels during treatment with iron sorbitol was excellent.
7) In experimental rabbits, more than 60% of Fe⁵⁹ injected in urine by 3 rd day after single intramuscular injection of Fe⁵⁹-labeled iron sorbitol (Fe 10mg/kg, 3.3μc/50mg/ml). At that time, only 3.5% of Fe⁵⁹ was left in the site of injetion. Less amount of Fe⁵⁹ was excreted in the urine in anemic rabbits than in normal rabbits, while utilization of Fe⁵⁹ by red blood cells was around 25% in anemic rabbits and it was higher than that in normal rabbits. Similar results were obtained following single intravenous injection of Fe⁵⁹-labeled iron sorbitol.
Concentration of Fe⁵⁹ of lymph nodes was considerably higher than that of other tissues.
It was however evidently lower as compared with that obtained in experimental rabbits injected with Fe⁵⁹-labeled iron dextran.

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Surveys were carried out on premenstrual and menstrual symptoms over a period from June 1958 to October 1961 with agrarian women, nurses, telephone operators, office employees, conductresses of bus, electric appliance workers, department store girls, watch-making workers, spinners, middle and higher school girls, rural elementary and middle school girls and other, totalling 10, 480, with the following results,
1. Premenstrual and menstrual symptoms are observed in 83.65% in ages of 2125 years. They were frequently encountered among telephone operators and conductresses of bus, and infrequently among rural elementary and middle school girls and agrarian women.
2. Premenstrual symtoms were observed in 56.61% in ages of 2135 years, especially in 2630 years, and more frequently among telephone operators and conductresses of bus and infrequently among rural elementary and middl eschool girls, and spinners. Ages of high frequency in different occupations were as follows; Less than 20 years among electric appliance workers and middle and higher school girls, and more than 30 years among office employees and agrarian women.
3. Menstrual symptoms were observed in 71.47% in ages of 1625 years, and frequent among electric appliances workers and telephone operators, and infrequent among office employees and agrarian women. Ages of high frequency in different occupations 2630 years among telephone operators, 2130 years among agrarian women, and 1625 years in the other.
4. Those having the symptoms only in the premenstrual period account for 12.17%, and were numerous among office employees and agrasian women, and few among watch-making workers and electric appliance workers. Ages of Highest frequency are 3645 years in the majority of occupations, but 3135 years among telephone operators and conductresses of bus, and 3650 years among agrarian women.
5. Those having symptoms from the premenstrual through the menstrual period accounted for 44.44%, and were numerous among telephone operators and conductress of bus, and few among spinners and agrarian women. Ages of high frequency was 1630 years in most occupations, but 2635 years among watch-making workers, 3640 years among office employees, and 4650 years among spinners.
6. Those having the symptoms only during menstruation accounted for 27.03%, and were numerous among spinners and electric appliance workers, but few among aggrarian women and other. Ages of highest frequency were below 20 years in the majority of occupations, but 2630 years among agrarian women, and 21 25 years among electric appliance workers and spinners.
7. A symptom was found in 16.35%, It occurs least frequently in 2125 years in general, but in 1620 years among middle and higher school girls and spinners, below 15 years among electric appliance workers, in 26-30 years among nurse, telephone operators and conductresses of bus, and in 3640 years among office employees.
8. As for the degree of premenstrual and menstrual symptoms, those who lay in bed or do not work owing to premenstrual symptoms account for only 4%, and there were no remarkable difference by age. They were relatively numerous among conductresses of bus. During the development of menstrual symptoms, only 1/3 led the usual lives, and the remainder had more or less disturbances. Those in 2130 years with serious menstrual symptoms who lay in bed or do not work were numerous among telephone operators and conductresses of bus, and percentage of adsentees were very high in these occupations. This was assumedly because many telephone operators and conductresses of bus considered, thanks to their union's propaganda, monthly holidays for woman workers as elementary right for them. Those who worked despite discomfort during menstruation were numerous among nurses.

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Water and electrolyte metabolism in rats were observed in each estrus cycle and after administration of sexual hormons, and the following results were obtained.
1 Circulating serum was significanty increased in estrus II. extracellular fluid in estrus I, and serum sodium in estrus I and II, while serum potassium and total serum protein were both significantly decreased in estrus II. (classification of estrus by Koyama and Nakao, 1954)
2 When rats were injected with PMS for 5 days, circulating serum was significantly decreases with daily dose of 100 i. u. and 500 i. u., while extracellular fluid was significantly increased with 100 i. u. and 500 i. u., serum sodium with 10 i. u., 100 i. u. and 500 i. u. and serum potassium and total serum protein with 10 i. u. and 500 i. u., There was no quantitative change in serum chloride and sodium chloride.
3 When rats were injected with HCG for 5 days, circulating serum was significantly decreased with daily dose of 10 i. u., 100 i. u. and 500 i. u., while extracellular fluid was significantly increased with. 10 i. u. and 100 i. u., serum sodium with. 10 i. u. and 100 i. u., serum potassium with 10 i. u. and 500 i. u., and total serum protein with 100 i. u. and 500 i. u., There was no quantative change in serum chloride and sodium chloride.
4 Rats were injected with PMS for 5 days and then with HCG for the succeding 5 days in the following daily doses. In group A. 10 i. u. each, in group B, 100 i, u. each, and group C, 500 i, u, each. Circulating serum was significantly decreased in all the groups, and so was extracellulr fluid in group A, while serum sodium and potassium were significantly increased in all the groups, and so were serum chloride and sodium chloride in group A, and total serum protein in all the groups.
5 When rats were injected with estradiol benzoate for 5 days, circulating serum was significantly decreased with daily dose of 0.02mg and 2mg, while extracellular fluid was significantly increased with 0.2mg, serum potasium with 0.2mg, and 0.4mg, serum chloride and sodium chloride with 0.2mg and 0.4mg, serum sodium and total serum protein with 0.02mg, 0.02mg and 0.04mg.
6 When rats were injected with progesterone for 5 days, circulating serum was significantly decreased with daily dose of 1 mg and 10mg, while serum sodium and potassium were significantly increased with 1 mg, 2 mg and 10mg, and serum chloride and sodium chloride with 1 mg and 2 mg. There was quantative change in extracellular fluid.
7 Estradiol benzoate and progesterone were jointly given for 5 days in the following dose, In group A, 0.02mg and 1 mg, in groupe B, 0.2mg and 2 mg, and in group C, 0.4mg and 10mg, respectively. Circulating serum was significantly decreased in group A and significantly increased in group C. Those were no remarkable change in extracelluar fluid, and serum chloride and sodium chloride. Serum sodium was significantly increased in group B and C, and total serum protein in all the groups. Whereas serum potassium was significantly decreased in group A, and significantly increased in group B.
8 Rats were injected with estradiol benzoate for 5 days and then with progesterone for the succeeding 5 days in the following doses. In group A, 0. 02mg and 1 mg; in group B, 0.2mg and 2 mg, in group C, 0.4 mg and 10 mg, respectively. Circulating serum was significantly decreased in group A, B and C, and serum potassium in group A, while serum sodium was significantly increased in group B and total serum protein in all the groups. There were no quantitative changes in extracellular fluid and serum chloride and sodium chloride.

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