Refractoriness of main and accessory fibres into Pacinian corpuscle has been measured with two impulses generated by mechanical stimuli or antidromical electrical stimuli.
1) The depression produced by a preceding stimulus of the Pacinian corpuscle, resulting from mechanical stimulus has been measured below 1 msec, and its value is smaller than transmission time from corpuscle to main fibre, and also than its of main fibre itself.
2) The absolute refractoriness of transmission from corpuscle to the main fibre is about 1. 3msec.
3) The absolute refractoriness of the main fibre itself is about 2 msec.
4) The recovery time for the complete transmission from corpuscle to the main fibre, needs 5 to 6 msec.
5) The absolute refractoriness of an accessory fibre is 2030msec.
6) The complete recovery time for C fibres needs about 200msec.
From above results we can consider that the most popular inserval of spontaneous discharge in main fibre is 5 to 6 msec and the accsssory fibres is 200msec.

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Effects of total body irradiation with 800 r of X-ray on the coneentration of free protoporphyrin and or catalase activity in the liver cells of rat were investigated. To elucidate the mechanism underlyng the changes in these values, synthetic ability of protoporphyrin or heme by the liver slice was studied in vitro incubation with glycine or ALA. In addition, difference between the effect of in vitro and in vi vo irradiation was investgated using frog crythrocytes. The results obtained were as follovs : (1) Concentrations. of both free protoanb eopro-porphyrin in the liver homogenate were decreased rapidly following the total body irrabiation and recovered to the control by 48th and 24th hour, respectively, Signi-ficant decreasc was observed in catalaSe activity in the liver homogenate following the irrabiation. An inhibitor to the catalase activity was detected neither ln plasma nor in the liver of the irradiated rats. (2) No increase in the amount of protoporphyrin in the liver homogenate following in vitro incubation with glycine even prior to the irradiation. Amount of the synthesized protoporphyrin in the liver slice following in vitro incubation with ALA tended to decrease after the irradiation. (3) Significant decreasc in in vitro heme-tynthetic activity of the liver slice either with glycine cr with ALA was observed following the irradiation. (4) From the results above described, it is suggested that a decrease in protoporphyrin content and catalase activity of the liver homogenate follohing the total body irradation results from both the inhibition of porphyrin- aud heme-synthesis and iucrease in the destruction rate of protoporphyrin, (5) In vitro protoborPhyrin- or heme-syuthesis by erythrocytes obtained from the frogs irradiated with 400 r of X-ray was reduced significantly, while no significant change was observed in heme-syuthesis by the frog erythrocytes irradiated in vitro with as high as 10, 000 r of X-ray.

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1. The absorption test of group-specific agglutinins was used to identify blood-group ABO activities of the erythrocytes and water-soluble fractions obtained from the pepsin digests of gastric mucosa in toads, Xenopus laevis and frogs, Rana catesbiana, and in addition, the group-specific precipitation reaction was used for these water-soluble fractions.
2. The erythrocytes of the toads do not contain any substances on blood-goup ABO system, whereas that of the frogs contain blood-group B substance.
3. It was confirmed that the toads have high AH antigenicity, and the frogs have high BH antigenicity with xeak A activity so far as the water-soluble fractions is concerned. The evidence that rabbits produce high titre of anti-A antibody after injection of the formcr's preparation and high titre of anti-B antibody after injection of the latter's one shows these water-soluble fractions to be an antigenic substance but not to be a hapten-like substance.
4. Treatment of the water-soluble fractions by four kinds of glycosidases elucidated that A antigenicity in the toads and B antigenicity in the frogs are determined by α-N-acetyl-D-galactosaminyl and α-D-galactosyl residues, respectively, accompanying low level of serological changes from A to H and from B to H.

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Experimental studies were performed on 28 hybrid dogs in order to elucidate changes of fibrinolysis during extacorporeal perfusion.
In clinical series of 43 cases undergoing open heart surgery, activations of the fibrinolytic enzyme system have been observed during and after extracorporeal perfusion.
Every episode of excessive oozing or bleeding following open heart surgery seems practically to be related to a significant increase in activity of this enzyme system. In the treatment for this fibrinolysis were dosed several anti plasmin agents.
Following results were obtained :
1) Fibrinolytic activity was advanced in more hypothermia than normothermia during the extracorporeal perfusion, and was not concerned to any kind of the diluent (Haemacel, Hart-mann pH 8).
2) Free and total plasmin activity was elevated to the muximum in about 30 minutes to 60 minutes after the beginning of perfusion in most cases. Activation of plasmin was detected in the above under surgical procedure.
3) And this investigation was naturally undertaken to evaluate new method of determining anti plasmin activity with the tool of SP-Plasma after standard plate.
4) Again fibrinolytic activity was advanced twice relatively one and six hours from postperfusion. Heparin was neutralized with protamine sulfate 4 mg/kg, which was verified to be optimum volume.
5) Dosages of the anti plasmin agents (EACA, AMCHA, Trasylol) were used in 10 minutes and 6 hours postperfusionally. Fibrinolysis was chiefly inhibited by means of EACA 0.1g/kg, AMCHA 0.04g0.06/kg.
6) Perfusuate combined heparinized blood with Ringer' solution + Haemacel + Mannitol was least hemolysic.
7) According to change the respiratory alkalosis, total plasmin activity was advanced. Negative correlation was found between fibrinolytic activity and a significant increase of the metabolic acidosis.

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Pacinian corpuscles of cat's mesentery have multiple innervation. Besides a myelinated large fibre and a fine accessory fibre, Aδ fibre enter the corpuscle. Aδ fibre is traced with histological and electropysiological means as an independent element in the mesenteric nerve, in the stalk of corpuscle and in the proximal segment inside the corpuscle.
This fibre conducts impulses at 12 m/sec. The threshold of it shows about 3 times that of the well-known mechanoreceptor axon of the sense organ.
This fibre ramificates two or three and loses the myeline sheath in the proximal segment. On the other hand the large fibre also loses the myeline after throughout the proximal segment. The interaction may be expected between the myelinated large fibre and the fine fibre.

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