In der vorliegenden Untersuchung haben wir zwei histochemischen Nachweismethoden von Polysaccharid und Schleim, Bauer' sche Methode und Überjodsäure-Schiff' Sche Reaktion, vergleichend studiert.
Als die histologische Methode geht die Überjodsäure-Schiff' sche Reaktion in verschiedenen Punken vor der Bauer' schen Methode, während diese einige nutzbare Vorteile hat. In Japan kann man jetztdie Üerjodsäure in Handel nicht gewinnen, so hahen wir statt dieser die mit derSalzsäure angesäuerte, 0.5%ige Kaliumperjodatlösung (KIO₄) benutzt und ganz gleiche Resultate wie bei der Überjodsäureanwendungbekommen. Wir möchten nun diese modifizierte Methode (''Kaliumperjodat-Schiff ' sche Reaktion'') für den histochsmischen Nachweis von Polysaccharid und Schleim empfehlen.

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I tried Feulgen Process, HCI-Giemsa method and thionine staining on Escherichia coli, Shigella dysenteriae, Salmonella paratyphi B. Diplococcus pneumoniae, Spreptococcus and Staphlococcus, and I could discern the nuclei of bacteria as Rohinow, Boivin and others informed.
With bacilli I generally noted 1-8 nuclei in one cytoplasm, ; with cocci 1 or 2. Often were observed images which were supposed to he under process of division of nuclei. In a variant, which was, obtained from Shigella dysenteriae through ultraviolet irradiation and which long remains fibrous, I noted many nuclei, and it is considered that only the divisioi of cytoplasm was impeded there.
In comparing Feulgen process, HCI-Giemsa method and thionine staining, the second was found best in that with it I can differentiate protoplasm from nuclei most distinctly.

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A boy, 12 years 2 months old, often complained of pain Regio hypochondrica sinistra for 2 months prior to death. While he was treated in the pediatrics dept, with diagnosis of aseariasis, was found tumor in the above mentioned site, and he was taken to the surgical dept, with esnspected colon tumor. The operation could not remove, the tumor beoause of firm adhesion, and he died.
Macroscopically were found a formation of ulcer in Flexra coli sinistra, thickening of intestinal wall and consequent narrowing of the lumen, and further; its firm adhesion to peritoneum and stomach wall.
Microscopic examination showed carcinoma simplex in which was noted mucus. The cancerous ulceration of the mucosa was most advanced in the periphery of the ulcer, and it is supposed to have originated there. The tumor also infiltrated into submucous coats of the other part of intestinal tract by contact spread and into lung in scirrhus by metastase.
According to the previous statistics, the rate of cancer developed in young men is about 0.5 %. It is rare but not extremely rare. But about cancer in young men's colon (excpt carcinoma of rectum) less than 20 cases have been reported according to the investingation, and so we report this as an uncommon cace.

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It is a essential f act that in the stimulation of a muscle or a single muscle fiber, there is threshold. The author, however, threw a doubt, if the treshold on cathodic contraction of a single muscle fiber would he found or not, and examined on the question in this work.
In the experiment a single fiber separated from M. sartoius etc. of the bullfrog was used. The employed method for stimulaton and recording of the contraction was the same, as was reported by Matsumoto Masao and Satake Y. Thus the author observed the relation between the grade of contraction and the strength of the applied current. The stimulating current wa right angular current or discharge of the condensor.
The result are the following :
With decrease of intelvity, the contraction became smaller and smaller untill it was not observed. This decreasing state of contraction is just like a cury approaching to its asymptote. These states of contraction were observed in cases of all kinds of stimulating current. Considering the results the author confirmed that there was no threshold for cathodic contraotion of the single muscle fiber. But as to the twich of a muscle or a single muscle fiber, of course the confirmation is not applied.

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From the reports of Matsumoto Masao and others, it will be assumed that there would be no refractory period about the cathodic contraction of a single muscle fiber. But there is no work, which intended to assure this problem experimentally. The author, therefore, carried out the experiment to determine if the cathodic contraction would have any refractory period or not.
In the experiment, a single fiber separated from M. sartorius etc. of the bullfrog was used and was fixed in the so-called Sugi' s isolating vessel. The stimulating current was led to the Ringer's solution of both sides in the isolating vessel by means of non-polarisable electrodes. The experimental results are : (1) When two induction shocks were applied successively with nterval of 1, 0 msec, then the contraction grade was compared with that of for single shock. So it was shown that the.contraction height was always greater in the former case than in the letter. Thus the refractory period would be smaller than 1, 0 msec, if it existed. (2) A single fiber was stimulated with two right auglar currents having same intensity but different duration (0, 01 and 0, 05 sec). So the contraction grade for the current of long duration were greater than that of for the short duration. The current of long duration can be considered as two currents which whrch were given successively with zero intervai. Accordingly, it is, possible to conclude that there is no refractory period.
On account of these experiments, it was cleared that there was no refractory period on the cathodic contraction of a single muscle muscle fiber.

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Sato measured suditory space with various methods; the results are summarized as follows :
(1) When an acoustic source mekes slowly rectilineal motion to various directions, the locuge of auditory image deviate convex to the head. In the horizontal plane, the degree of deviation is most intensive at the side and back. In the frontal plane, it is similarly intensive at the side and foot, dut at the upside the deviation is not to be seen. In the median plane the locus is indistinct. (2) With the increase of the distance from the ear in hewing two acoustic images gradually fuse into one and five periods can be classified in them. Linking the points of same period in various directions I got a horizontal component of auditory space which is flat at the side and back. In the same way the frontal and median components were measured. (3) From three sound images which are corresponding to three sound sources on a spherical surface and audible at equal intervals, I got a tangent at the middle sound image by the bisection method and measured the auditory space of phenomenal type. Its horizontal component is flat at the side, the frontal one is flat at the side and the median one has a tendency to be flat in front and lengthened postero-inferiorly. (4) Many sound sources which are formed in a straight line at the various position in the hosizontal plane can be heard not as a rectilineal image but as two groups of sound images which lie on the left and right side or in front and at the back and are of huge comma shape. Using them as a indicator the auditory space was measured just in the same way as in (2). From the many sound sources formed. rn a circle quite similar phenomena were to be seen.

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