The KITAKANTO Medical Journal

GENETIC ANALYSIS OF BACTERIOCIN AND CONJUGATION-RELATED GENES OF BACTERIOCIN PLASMID pPD1 IN ENTEROCOCCUS FAECALIS

ERI WAKAMATSU, HARUYOSHI TOMITA, SHUHEI FUJIMOTO, KOICHI TANIMOTO

1995 Volume 45 Issue 2 Pages 139-154
Published: March 01, 1995
Released on J-STAGE: October 15, 2009

DOIhttps://doi.org/10.2974/kmj1951.45.139

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Conjugative bacteriocin plasmid pPD1 (59kb) of Enterococcus faecalis has been mapped physically by the relational clone method. pPD1 consisted of ten EcoRI fragments with corresponding molecular sizes of 22.5, 13.0, 9.0, 6.9, 5.0, 1.0, 0.6, 0.36, 0.28, and 0.23kb, and in the order of A G C E B F D J H I in the clockwise orientation. Transposition of Tn917 (Em) to pPD1 gave rise to mutants altered in bacteriocin expression. Transposon mutagenesis and the expression of bacteriocin of the relational clones revealed the region of the pPD1 map required for bacteriocin synthesis. Tn917 insertions within a certain region resulted in constitutive clumping, and the DNA sequence was analyzed in the region. The region of pPD1 spans about 8kb and was found to contain ten open reading frames (ORF). Significant homologies of sequence were identified between seven ORFs and genes in the regulatory region of pAD1 and pCF10, respectively.

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POSTNATAL DEVELOPMENT OF PENTYLENETETRAZOLE-INDUCED c-fos mRNA IN DISCRETE BRAIN AREAS OF THE RAT

AKIHIKO OSHIMA

1995 Volume 45 Issue 2 Pages 155-162
Published: March 01, 1995
Released on J-STAGE: October 15, 2009

DOIhttps://doi.org/10.2974/kmj1951.45.155

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Recently detection of c-fos induction after external stimulation has been widely performed for functional mapping. In this study we examined the induction of c-fos mRNA after systemic administration of a chemical convulsant, pentylenetetrazole (PTZ), in four brain areas of the developing rat.
Subcutaneous injection of PTZ (50mg/kg) induced occasional brief myoclonic jerk from postnatal day 8 to day 49, and significantly enhanced c-fos mRNA expression in the neocortex at postnatal day 8, 23 and 49, compared with saline administration. The level of c-fos mRNA expression in the PTZ-injected animals increased progressively from day 8 to 23 and markedly from day 23 to 49. In the piriform cortex, a PTZ-induced increase in the proto-oncogene expression was observed only at day 49 postpartum. In contrast, the levels of c-fos mRNA were unchanged after administration of the convulsant in the hippocampus and the striatum throughout day 8 to 49.
The regional variation in the development of PTZ-induced c-fos mRNA expression might parallel a change in the size or distribution of neuronal populations involved in induction of the phenotypically identical seizure. Alternatively, it might reflect an alteration in the neural circuitry associated with such long-lasting effects of epileptic seizure as kindling phenomenon.

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ENHANCEMENT BY ZINC ION OF CHLORIDE CURRENTS GENERATED THROUGH GLYCINE RECEPTOR CHANNELS IN RAT CULTURED NEURONS AND XENOPUS OOCYTES

TAKEHIKO MAJIMA

1995 Volume 45 Issue 2 Pages 163-171
Published: March 01, 1995
Released on J-STAGE: October 15, 2009

DOIhttps://doi.org/10.2974/kmj1951.45.163

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The effects of Zn²⁺ on the glycine receptor channels expressed in rat cultured neurons and Xenopus oocytes injected with complementary RNA encoding the adult form of glycine receptor subunit (α1) were examined. Application of glycine at 100μ M caused generation of inward currents when the neurons were voltage-clamped a-60mV. In the presence of Zn²⁺ at 1μM, the glycine-induced currents increased currents by up to 2-fold compared with the control responses. The augmented responses were reversed within 2 minutes after washing out Zn²⁺. Zn²⁺ alone did not cause any change in membrane currents. In the experiments with oocytes expressing α1 homomeric glycine receptors, Zn²⁺ (0.1-10μM) enhanced the glycine-induced currents by up to 2 to 5-fold compared with the control responses in a reversible manner. Other divalent cations such as Cd²⁺, Ni²⁺, Co²⁺, Mn²⁺, Mg²⁺ and Cu²⁺ at low concentrations (1-10μM) had no effect on the glycine currents. Experiments using a combined application of Zn²⁺ with glycine revealed that the action site of Zn²⁺ is on extracellular space. These results suggest that Zn²⁺ binds to a specific site on the α subunit of the glycine receptor to positively regulate the channel activity.

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CLINICAL RESULTS OF TOTAL PROSTATECTOMY

TAKANORI SUZUKI, KYOICHI IMAI, MASAMICHI HAYASHI, KOHEI KUROKAWA, YOSH ...

1995 Volume 45 Issue 2 Pages 173-177
Published: March 01, 1995
Released on J-STAGE: October 15, 2009

DOIhttps://doi.org/10.2974/kmj1951.45.173

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Total prostatectomy was performed in 15 patients with prostate cancer during a period of ten years, and the clinical results were analyzed. The age ranged from 60 to 71 years (average 65.5 years old), and the clinical stage was stage B in 14 patients and C in one. Prostatectomy was performed using the retropubic and antegrade approach, and partial resection of the pubic bone was done in 7 patients. After prostatectomy, lymphadenectomy of the regional lymph nodes was performed.
The operations averaged 4 hours and 49 minutes, and the blood loss was average 1760g. Postoperative complications were inflammation of the pubic bone in two patients, urethral stenosis in one and a mild stress incontinence in one. Of the 7 patients in stage B2, pelvic lymph node metastasis was observed in one patient, carcinoma invasion at the prostatic apex was in three, and the prostatic capsule invasion in two. After prostatectomy, six patients had no treatment, while the patients with pT3 and lymph node metastasis had received radiation and hormonal therapy. Over all survival and cause-specific survival rates at 5 years in all patients were both 100%, and there was no tumor recurrence in any patient.

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A CLINICAL STUDY OF PRIMARY GASTRIC LYMPHOMA

SHUN-ICHI SHIMANO, KAYOKO MURAYAMA, TOSHIKAZU AKIYA, ICHIRO OHKI, TAKA ...

1995 Volume 45 Issue 2 Pages 179-184
Published: March 01, 1995
Released on J-STAGE: October 15, 2009

DOIhttps://doi.org/10.2974/kmj1951.45.179

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Nineteen patients with primary gastric lymphoma were diagnosed and treated between 1981 and 1994. Clinical stage as evaluated by the Ann Arbor classification was I in 4 cases, II in 7 cases, III in 1 case, and IV in 7 cases. According to the Working Formulation 16 patients had intermediate-grade lymphoma. There was one case of Hodgkin's disease. CRP at admission, was positive in 11 of 15 patients, but LDH was abnormal in only 3 of 19 patients. Treatment included surgery (5) in 5 cases, chemotherapy after surgery (S+CT) in 5 cases and chemotherapy (CT) in 6 cases. Complete remission (CR) was achieved in 11 of 18 patients, that is, S in 5, and S+CT and CT in 3 each. Median survival time by Kaplan-Meier method was 20.9 months. There was only one death among patients who achieved a state of CR during the observation period. We consider that CR patients have a good prognosis.

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LONG-TERM SURVIVAL OF PATIENTS WITH NON-RESECTED ADVANCED NON-SMALL CELL LUNG CANCER

TAISUKE NOMOTO, SATOSHI TSUCHIYA, HIDEHIKO NAKANO, SATORU WATANABE, TA ...

1995 Volume 45 Issue 2 Pages 185-190
Published: March 01, 1995
Released on J-STAGE: October 15, 2009

DOIhttps://doi.org/10.2974/kmj1951.45.185

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We reviewed the outcome and long-term survival of 159 patients with non-resected advanced non-small cell lung cancer who were treated by chemotherapy and/or radiotherapy from 1987 to 1989. Three-year survival was achieved in 14 cases, 8.8% of all cases. there were no differences in the incidence of three-year survival, According to age and sex, but most of long-term survivors had good performance status (PS 0-1, 13 cases, 92.9%), and the rate of squamous cell carcinoma case (8 cases, 57.1%) was higher than the rate of one in all cases. Patients were treated with chemotherapy (5 cases), radiotherapy (1 case) and chemotherapy+ radiotherapy (8 cases), and the response rate of long-term survivors was 42.9%. Tumor markers (CEA, SCC, SLX) of long-term-survivors were almost normal, but in higher cases, the levels were decreased after treatment.

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A CASE OF LEFT ADRENAL ALDOSTERONOMA AND DUPLICATED INFERIOR VENA CAVA : INVERSE BLOOD FLOW IN THE LEFT VENA CAVA AFFECING ALDOSTERONE CONCENTRATION IN THE LEFT RENAL VEIN

YASUSHI SUZUKI, HIRONORI YOKOYAMA, TATSUYA KATO, FUMIO NAGANUMA, SHOUI ...

1995 Volume 45 Issue 2 Pages 191-197
Published: March 01, 1995
Released on J-STAGE: October 15, 2009

DOIhttps://doi.org/10.2974/kmj1951.45.191

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We report a case of primary aldosteronism and duplicated inferior vena cava (IVC) with an unusual concentration gradient of aldosterone in the left renal vein. A 53-year-old man with primary aldosteronism was admitted to Gunma University Hospital. Abdominal CT showed a left adrenal mass lesion (15mm) and duplicated IVC. The plasma aldosterone concentration was extremely high at each venous sampling. However, the aldosterone concentration was higher in the distal portion of the left renal vein than in the proximal portion. Left adrenal venography showed that blood flow in the duplicated left IVC was directed from the left renal vein to the left common iliac vein. This implied that the inverse blood flow in the left IVC caused a high aldosterone level in the distal left renal vein.

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SUCCESSFUL PTCA IN A CASE OF ANOMALOUS ORIGIN OF THE LEFT CIRCUMFLEX CORONARY ARTERY

FROM THE RIGHT SINUS OF VALSALVA

TSUTOMU HOSOI, YUKIO KOYAMA, SHOUICHI TANGE, HITOSHI ADACHI, HIROYUKI ...

1995 Volume 45 Issue 2 Pages 199-204
Published: March 01, 1995
Released on J-STAGE: February 09, 2010

DOIhttps://doi.org/10.2974/kmj1951.45.199

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We experienced a case of anomalous origin of the left circumflex coronary artery (LCX) from the right sinus of Valsalva successfully treated by PTCA. A 48-year-old man had had recurrent exertional chest pain for a year. Physical examination, laboratory tests, electrocardiography, chest X-ray and echocardiography showed no abnormality. Coronary angiographic study revealed anomalous origin of LCX from the right sinus of Valsalva and bilateral coronary artery-pulmonary artery fistula. LCX arising aberrantly from the right sinus of Valsalva showed 90% stenosis in its proximal portion. PTCA to LCX was carried out, and an excellent result was obtained. PTCA is useful even if the coronary artery arises aberrantly from the sinus of Valsalva. This case is very rare in terms of the combined bilateral coronary artery-pulmonary artery fistulas and anomalous origin of coronary artery.

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[title in Japanese]

1995 Volume 45 Issue 2 Pages 205-207
Published: March 01, 1995
Released on J-STAGE: October 15, 2009

DOIhttps://doi.org/10.2974/kmj1951.45.205

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[title in Japanese]

1995 Volume 45 Issue 2 Pages 209-230
Published: March 01, 1995
Released on J-STAGE: October 15, 2009

DOIhttps://doi.org/10.2974/kmj1951.45.209

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[title in Japanese]

1995 Volume 45 Issue 2 Pages 231-241
Published: March 01, 1995
Released on J-STAGE: October 15, 2009

DOIhttps://doi.org/10.2974/kmj1951.45.231

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