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Volume 42 (1992)
- Issue 6 Pages 527-
- Issue 5 Pages 425-
- Issue 4 Pages 331-
- Issue 3 Pages 165-
- Issue 2 Pages 69-
- Issue 1 Pages 1-
Volume 42, Issue 2
Displaying 1-12 of 12 articles from this issue
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HIROYUKI MOCHIZUKI1992 Volume 42 Issue 2 Pages 69-76
Published: March 01, 1992
Released on J-STAGE: October 21, 2009
JOURNAL FREE ACCESSThe effects of arachidonic acid (AA) on short circuit current (Isc) across primary cultures of canine tracheal epithelium were studied. Cells were grown with an air interface in serum-free medium on filters. Isc was measured by the Ussing chamber technique. The addition of AA increased Isc by 27.8±5.2μA/cm2 (n=8). When indomethacin (INDO) and nordihydroguaiaretic acid (NDGA) were present, addition of AA reduced Lsc by -4.4±1.0μA/cm2 (n=10). The actions of AA were unaffected by amiloride. A combination of INDO, NDGA and AA, significantly reduced isoproterenol (ISO) -induced stimulation. In ISO reactions of INDO and NDGA pretreated samples, cAMP levels did not differ between with and without AA. Furthermore, AA suppressed the 125I efflux stimulated by forskolin and CPT-cAMP. These findings show a direct inhibitory effect of AA on apical membrane chloride channels.View full abstractDownload PDF (1104K) -
SHOICHI TERADA, YOKO KOBAYASHI, TAKAO SHINOZAWA1992 Volume 42 Issue 2 Pages 77-85
Published: March 01, 1992
Released on J-STAGE: October 21, 2009
JOURNAL FREE ACCESSMany trophic factors have previously been reported. However, few authors have reported on growth inhibitory factors, such as cell growth inhibitory factors purified from conditioned cell culture media or sialoglycopeptide obtained by protease E treatment of cerebral cortex homogenates.
For the elucidation of PC-12 cell growth inhibitory factor, trypsinization, heat treatment and ion-exchange column chromatography were carried out. The cell growth inhibitory activity was found in the CM-TOYOPEARL bound fraction. One μg of protein from the above fraction inhibited PC-12 cell growth about 30% and the inhibition was reversible. Trypsinization was necessary to obtain the inhibitory protein.
Our inhibitory protein specifically inhibits the growth of PC-12 cells. The growth of Neuro2 a and A375 cells was not inhibited. This inhibition spectrum is quite different from that of sialoglycopeptide, which inhibited the growth of both PC-12 and Neuro2a cells.View full abstractDownload PDF (1501K) -
SUSUMU ISHIKAWA1992 Volume 42 Issue 2 Pages 87-96
Published: March 01, 1992
Released on J-STAGE: October 21, 2009
JOURNAL FREE ACCESSThis study was designed to clinically and experimentally elucidate the causes of atrial fibrillation (Af) and hemodynamic changes following open-heart surgery. In the clinical study of 94 patients aged 30 or above undergoing atrial septal defect (ASD) repair, postoperative outbreak of Af significantly related to advanced age above 50, large preoperative left atrial diameter above 40 mm, and postoperative overhydration. In the experimental study using electrically induced Af models in dogs, a fall in mean arterial pressure, a decrease in cardiac output and elevations in central venous and left atrial pressures were observed. During Af, aerobic metabolism of the cardiac muscle was accelarated. The electrical stimulation threshold for experimental Af significantly fell with elevations in atrial pressure. In conclusion, Af after open-heart surgery may relate to preoperative changes in the stimulation conduction systems which are indicated by left atrial dilatation and postoperative atrial distension due to overhydration.View full abstractDownload PDF (1680K) -
YUKIO MIYAMOTO, IZUMI TAKEYOSHI, SEIJI NAKAMURA, YOSHIBUMI TANAHASHI, ...1992 Volume 42 Issue 2 Pages 97-101
Published: March 01, 1992
Released on J-STAGE: October 21, 2009
JOURNAL FREE ACCESSIn a retrospective study, lymph node metastases on gastric cancer, especially the metastatic rate to the 4sb lymph nodes, were examined in 527 patients who underwent curative resection between August 1980 and December 1990.
The results were as follows : 1) No metastasis to the 4sb lymph nodes was found in patients with early cancer of lower region. 2) The rate of metastasis to the 4sb lymph nodes was 2% in patients with early cancer of the middle portion. 3) The metastatic rate to second group lymph nodes in patients with early cancer on the lower region was from 2 to 7%. 4) Metastases to the 4sb lymph nodes were frequently observed in patients with advanced cancer on the each region.
It is suggested that patients with early gastric cancer of lower region may undergo a modified operation without resection of the 4sb lymph nodes. The modified operation may provide reduction of the size of gastric resection, preserving the proximal portion of the greater omentum, decreasing the time for surgery, anesthesia and reducing blood transfusion volumes, thus resulting in improved QOL with introduction of this surgical procedure.View full abstractDownload PDF (753K) -
HITOSHI ADACHI, AKIRA HASEGAWA, SHIGETO NAITO, NORIYUKI SAITO, AKIRA O ...1992 Volume 42 Issue 2 Pages 103-109
Published: March 01, 1992
Released on J-STAGE: October 21, 2009
JOURNAL FREE ACCESSAlteration of the activity of the β-adrenergic receptor pathway was investigated in rats with chronic myocardial infarction. Cardiac β-adrenergic receptor density (Bmax) and β-adrenoceptoradenylate cyclase activity (cAMP production in the cardiac membrane with isoproterenol-stimulation) were measured. The ratio of left ventricular weight to body weight was increased one week after myocardial infarction. Left ventricular end-diastolic pressure and heart rate increased from the 1st week and had further increased by the 10th week. The density ofβ-adrenergic receptors increased from the 1st week, but had diminished by 10th week. Both basal and isoproterenol-stimulated cAMP production in the cardiac membrane decreased from the 1st week. These results indicate that heart failure is compensated for from the 1st to the6th week of chronic myocardial infarction, but not at the 10th week. Elevation of theβ-adrenergic receptor density seems to be one of the most important compensatory mechanisms in the chronic heart failure.View full abstractDownload PDF (1090K) -
TOSHIO KONISHI, TAMIUIKI OHBAYASHI, TATUO KANEKO, TOSIHIDE OHKI, YASUM ...1992 Volume 42 Issue 2 Pages 111-115
Published: March 01, 1992
Released on J-STAGE: October 21, 2009
JOURNAL FREE ACCESSErythropoietin was used preoperatively in three cardiac surgery cases. Operative procedures were coronary artery bypass grafting in two cases, and valve replacement in one.
The reticulocyte count and blood hemoglobin level had begun to increase a week after administration of erythropoietin, while autologous blood donation was also performed before the operation in two cases. No homologous blood transfusions were needed because of the increased hemoglobin level or autologous blood deposit. No adverse effects were recognized in any of the three cases and all were discharged without complications.
It was concluded that erythropoietin administration combined with autologous blood donation in advance of the operation offers an excellent means of preventing homologous blood transfusion. Further study concerning the appropriate application of erythropoietin seems to be required.View full abstractDownload PDF (746K) -
HIDEOMI WATANABE, IVAN R. NABI, AVRAHAM RAZ1992 Volume 42 Issue 2 Pages 117-124
Published: March 01, 1992
Released on J-STAGE: October 21, 2009
JOURNAL FREE ACCESSPhagokinetic assay demonstrated that B16-F1 melanoma cells cultivated in vitro as spheroids on a non-adhesive substrate produce more autocrine motility factor (s) as compared to cells cultured as a monolayer. Cells grown as spheroids showed more motile activity stimulated by conditioned media than cells grown as a monolayer. Fluorescence activated cell sorter analysis showed that cells cultivated as spheroids are less stained with the 3F3A monoclonal antibody directed against a Mr 78, 000 glycoprotein, which is identified as a membrane receptor for autocrine motility factor, compared to cells grown as a monolayer. These results suggest that enhancement of the production of autocrine motility factor (s) and regulation of its receptor endocytosis play an important role in the enhanced motility of cells grown as spheroids which enhance their ability to colonize the lung.View full abstractDownload PDF (3022K) -
A STUDY OF LIVING AND POST MORTEM CASESTOMIO KOBAYASHI, KATSUHIKO SONE, TAKASHI KOSUDA, KAZUKI HIRONO, MASAHI ...1992 Volume 42 Issue 2 Pages 125-132
Published: March 01, 1992
Released on J-STAGE: October 21, 2009
JOURNAL FREE ACCESSPrimary endocardial fibroelastosis (EFE) is a cardiomyopathy of unknown origin that affects infants and young children.
We treated 6 cases of EFE. 4 cases are alive and 2 cases died. The important prognostic factors are : duration of untreated period, low voltage and extremely high voltage in the left chest leads of the electrocardiogram and a high degree of fibroelastosis histologically.
The LV functions of the 3 living cases have not improved on echocardiography despite intensive treatment, so careful follow up is necessary.View full abstractDownload PDF (3325K) -
MAKIZO HIRABAYASHI, SHIZUKA OKADA, SAKUTARO TADOKORO1992 Volume 42 Issue 2 Pages 133-143
Published: March 01, 1992
Released on J-STAGE: October 21, 2009
JOURNAL FREE ACCESSEffects of repeated subcutaneous administration of cocaine (COC) at doses of 10, 20 or 40 mg/kg at intervals of 1-7 days 5 times in mice placed individually into tilting activity cages of a sufficient size (20 cm in diameter and 15 cm height) were examined. A progressive enhancement of the ambulation-increasing effect was noted for 3-4 hrs after each administration, indicating that reverse tolerance had occurred. The doses of 10-20mg/kg and intervals of 3-7 days were optimal for producing clear reverse tolerance. The enhancement of the effect progressed rapidly and the maximal reverse tolerance was observed at the 3rd-4th administration and this enhancement lasted at least one month. However, higher doses of COC (40mg/kg) caused stereotypy concurrent with preconvulsive signs of short duration that were enhanced by serial administration. In contrast, pretreatments with the drug in a narrow environment (glass jar with a 5.5cm diameter and 15cm height) did not cause any enhancement. Cross-reverse tolerance occurred between COC and methamphetamine.View full abstractDownload PDF (1763K) -
NAOHUMI SATOU, HIDETADA AOYAGI, OSAMU TESIGAWARA, MINORU KANAMARU, KEI ...1992 Volume 42 Issue 2 Pages 145-150
Published: March 01, 1992
Released on J-STAGE: October 21, 2009
JOURNAL FREE ACCESSIt is well known that congenital abnormalities of the pancreatico-choledocho-ductal junction caused of congenital choledochal cyst and biliary malignancy. Recently, routine examination such as ERCP or PTC have often disclosed such anomalies. We have experienced five such cases in one year, since January of 1991, and performed biliary reconstructive surgery with bile duct resection. The basic method of biliary reconstruction is Roux-en-Y hepaticojejunostomy. In this report, we describe indications for selection of the reconstructive method and postoperative biliary drainage.View full abstractDownload PDF (1889K) -
NORIYUKI SATO, YOSHIYUKI UOZUMI, SHINTARO YANO1992 Volume 42 Issue 2 Pages 151-157
Published: March 01, 1992
Released on J-STAGE: October 21, 2009
JOURNAL FREE ACCESSPrimary IgA nephropathy is a common form of glomerulonephritis that leads to end-stage renal disease at variable rates in 10% to 30% of patients. A progressive course is observed in some nephrotic cases, especially when moderate to severe mesangial proliferative changes and extensive fibrocellular crescents are present.
We describe here a 18-year-old female patient with IgA nephropathy showing nephrotic syndrome associated with mild renal dysfunction. Renal biopsy showed moderate mesangial proliferation and fibrocellular crescents (3/5 glomeruli). Strong mesangial IgA deposits were seen with immunohistological techniques. This patient responded to a combined regimen of prednisolone, ticlopidine hydrochloride, and dipyridamole with complete disappearance of proteinuria and hematuria, and her renal function normalized. This case demonstrated that combined therapy with prednisolone, ticlopidine hydrochloride, and dipyridamole should be attempted in patients with nephrotic IgA nephropathy with renal dysfunction.View full abstractDownload PDF (2066K) -
HIROKO IJIMA, TOSHIJI SHITARA, NORIYUKI SUETAKE, SHIN-ICHIRO YUGAMI, M ...1992 Volume 42 Issue 2 Pages 159-163
Published: March 01, 1992
Released on J-STAGE: October 21, 2009
JOURNAL FREE ACCESSA case of infantile genetic agranulocytosis (Kostmann type) is reported. The mechanism of impaired granulopoiesis in this patient was also evaluated. The peripheral blood picture soon after birth was compatible with absolute neutropenia. Bone marrow aspiration showed no polymorphonuclear neutrophils beyond the promyelocytic stage. Stimulation with epinephrine or hydrocortisone produced no increase in granulocytes. He was isolated in a clean room, and placed on prophylactic administration of sulphamethoxazole and trimethoprim, thereby preventing serious infections. As it has been suggested that T-cells play an important role in the pathogenesis of Kostmann-type neutropenia, anti-lymphocyte globulin was tried. No increase in granulocytes was obtained, however. Administration of other drugs has so far produced no increases in granulocyte levels.View full abstractDownload PDF (652K)
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