1. An antibody, specifically reacting with R
+ strains, was demonstrated in an immune serum obtained by injecting a rabbit either with R
+100-1 strain of
E.coli or with R
+ strain of
Salmonella2.
E. coli r
+ antiserum and
S r
+ antiserum were absorbed with
E.coli R
+ strain and
Salmonella R
+ strain, alternately. Thc result indicated that r
+ antigens of
E.
coli and
Salmonella wonld have their specific antigenicities as well as common antigenicity.
3. Bacterial suspensions of
E.
coli R
+ and
Salmonella R
+ strain were treated with a blendor, and the whole was centrifuged.
Then the supernatant was recentrifuged twice. first at 18, 000 r. p. m.and then at 38, 000 r.p.m. And r
+ antigenicity was demonstrated in the fraction which was sedimented by the 38, 000 r p.m.centrifugation.
4. The r
+ antigenicity of the living cells or of its ultracentrifuged fraction was lost when it was heated at 100°C for 30 min., or treated with proteinases such as trypsin, ficin or bromelin.
It is consequently assumed that the r
+ antigen would have proteinic properties.
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