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Volume 46 (1996)
- Issue 6 Pages 457-
- Issue 5 Pages 349-
- Issue 4 Pages 301-
- Issue 3 Pages 203-
- Issue 2 Pages 89-
- Issue 1 Pages 1-
Volume 46, Issue 2
Displaying 1-12 of 12 articles from this issue
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HARUYOSHI TOMITA1996 Volume 46 Issue 2 Pages 89-102
Published: March 01, 1996
Released on J-STAGE: October 15, 2009
JOURNAL FREE ACCESSThe conjugative plasmid pYI17 (57.5kb) isolated from Enterococcus faecalis YI17 confers a pheromone-response on the host and encodes the bacteriocin 41 (Bac41) gene. Bac41 is active against E.hirae 9790, E.faecium and Listeria monocytogenes. pYI17 was physically mapped by restriction enzyme analysis and the relational clone method. Deletion mutants and sequence analyses of the EcoRI fragment B cloned from pYI17 revealed that a 1.0kb fragment contained the bacteriocin gene (bacA) and an immunity gene (bacB). This fragment induced bacteriocin activity in E.faecalis OG1X and E.hirae 9790. The bacA gene is located on the pYI17 physical map between 3.37kb and 3.57 kb, and bacB is located between 3.59 kb and 3.87 kb. bacA encodes 67 amino acids and bacB encodes 94 amino acids. The deduced amino acid sequence of the BacA protein contained a series of hydrophobic residues typical of a signal sequence at its amino terminus. The predicted mature BacA protein (43 amino acids) showed sequence homology with the membrane active class II bacteriocins of lactic acids bacteria (LAB). Analysis of Tn5 insertion mutants and their transcripts indicated that these genes are transcribed as an operon composed of bacA, bacB, and an open reading frame located downstream of bacB designated as ORF3.View full abstractDownload PDF (3065K) -
KOICHIRO FUJITA1996 Volume 46 Issue 2 Pages 103-111
Published: March 01, 1996
Released on J-STAGE: October 15, 2009
JOURNAL FREE ACCESSSince the myosin light chain kinase (MLCK) is found in association with actin filaments in skeletal muscle, the author investigated the regulatory role of the actin-binding activity of MLCK from chicken breast muscle in the actin-myosin interaction. The amount of MLCK bound to actin increased with an increase in the concentration of MLCK to the 10-7M level. The actin-activated ATPase activity of myosin in both the phosphorylated and the unphosphorylated form was reduced to the same extent with an increase in the concentration of MLCK. Since MLCK showed little binding to myosin, the inhibition of the ATPase activity was attributed to the actin-binding activity of MLCK and not to its phosphorylating activity. The actin-binding activity of MLCK was obscured when Ca2+ and calmodulin (Ca2+-CaM) were present, and the inhibition by MLCK of the ATPase activity was also abolished. The inhibition of the ATPase activity and the recovery from the inhibition upon addition of Ca2+-CaM were confirmed by a in vitro motility assay in which ATP-dependent movement between actin and myosin can be detected directly. The author has discussed the domain that is responsible for the inhibitory effect.View full abstractDownload PDF (1470K) -
AKIO NAKAMURA1996 Volume 46 Issue 2 Pages 113-129
Published: March 01, 1996
Released on J-STAGE: October 15, 2009
JOURNAL FREE ACCESSCalcium-dependent inhibitory factor (CIF) is a major calcium binding protein in the plasmodia of a lower eukaryote, Physarum polycephalum, and exerts an inhibitory effect on protein kinases of Physarum such as the myosin light chain kinase. A cDNA encoding CIF was isolated, expressed in E. coli, and characterized. The CIF contained four EF-hand motifs and an Arg-Gly-Asp sequence. The molecular mass of CIF was calculated to be 40, 508 deduced from the nucleotide sequence. This CIF assembles in calcium-dependent manner. Polyclonal antibodies were raised against recombinant CIF. Using this antibody, I found that CIF was located in not only cytoplasm but also in nucleolus of Physarum plasmodia.
In an attempt to extend these observations in vertebrate cells, an immunofluorescence study of cultured vertebrate cells, CHO-K1 and 10 T 1/2 was performed. This antibody stained nucleoli of the both cell Types. Western blot analysis of the nuclear fraction of these cells indicated that the antibody recognized a protein band of 40 kDa, that is similar in mass to that of CIF in Physarum. The antibody also cross-reacted with polymorphonuclear leukocytes. The subcellular localization of this protein in these cells was found to be predominantly in the cytoplasm. The role of this protein in nucleolus has been discussed with special reference to cell cycle and motility.View full abstractDownload PDF (6401K) -
YINHUAN XUE1996 Volume 46 Issue 2 Pages 131-137
Published: March 01, 1996
Released on J-STAGE: October 15, 2009
JOURNAL FREE ACCESSDevelopmental changes in the expression of mRNAs encoding inhibitory glycine receptors in the chick CNS were analyzed. Glycine induced responses in Xenopus oocytes injected with mRNA of embryonic spinal cords (E10 and 20) and of brains (E10, 20 and P10) were barely detectable, while small glycine responses were observed in oocytes injected with mRNA of postnatal spinal cord (P10 and 20). In Northern blot analysis, no signal was observed in mRNAs from either spinal cord or brain of various ages when rat α2 subunit cDNA was used as probe. Southern blot analysis revealed that the chick genomic DNA fragments correspoding to the rat α2 subunit cDNA probe were detectable, indicating that in the chick CNS, α2 subunit-like transcripts are not expressed in spite of the prenence of genomic information. These results suggest that the mechanism underlying the expression of GlyR in the avian CNS might be different from that in the mammalian CNS.View full abstractDownload PDF (1501K) -
TETSUYA OTAKE1996 Volume 46 Issue 2 Pages 139-146
Published: March 01, 1996
Released on J-STAGE: October 15, 2009
JOURNAL FREE ACCESSRecently seen an increasing number of patients with osteoporosis has been observed that presents as a chronic illness, particularly to the elderly female patients. It is important not only to treat pain but to follow up bone mass changes to prevent decrease in bone mass. On a measurement of bone mass in patients with osteoporosis, author has emploied the Microdensitometry (MD) method. In this study, author examined changes of bone mass and the severity of pain in patients on long-term therapy with Gui-zhi-jia-shu-fu-tang, Ipriflavone and Oxaprozine.
In 18 cases given Gui-zhi-jia-shu-fu-tang, the initial bone mass data were as follows : metacarpal-index (MCI), 0.39±0.08; bone density (ΣGS/D), 2.07±0.32. No difference or increase from the initial values after three, six and nine months of treatment was observed.
In 22 cases given Ipriflavone, the initial data were : MCI, 0.39±0.07;ΣGS/D2.07±0.33. The results obtained after three, six and nine months of treatment showed no difference or increase from the initial values.
In 11 cases given Oxaprozine, the initial MCI and ΣGS/D values were 0.40±0.06 and 2.08±0.33, respectively. Furthermore, these data were 0.36±0.05 and 1.92±0.26 after nine months, showing a significant decrease.
The severity of pain was equally reduced by treatment with Gui-zhi-jia-shu-fu-tang or Oxaprozine. However, only the bone mass in patients given Oxaprozine was decreased.
The group given Gui-zhi-jia-shu-fu-tang showed suppression of bone loss, demonstrating the effectiveness of this agent in maintaining and improving bone mass.View full abstractDownload PDF (1536K) -
TAKANORI SUZUKI, KOHEI KUROKAWA, HIROOMI HIGASHI, KAZUHIRO SUZUKI, YOS ...1996 Volume 46 Issue 2 Pages 147-154
Published: March 01, 1996
Released on J-STAGE: October 15, 2009
JOURNAL FREE ACCESSTransurethral balloon laser thermotherapy (TUBAL-T) was performed in 30 patients with benign prostatic hyperplasia (BPH), and the mechanism of TUBAL-T was assessed by a pathological study. The urethra was cooled by a urethral cooling system during the treatment, and the prostatic tissue at 5 to 10mm depth from the urethral surface was heated to more than 45 degrees C. The depth of tissue damages ranged from 10 to 20mm from the urethral surface by CT or MRI. Pathological studies immediately after the treatment indicated the detachment of prostatic epithelia from the basement membrane, nuclear degeneration and coagulonecrosis of the smooth muscle cells, swollen endothelial cells, and stasis of leukocytes in the vessels. The mechanism of TUBAL-T was thought to be an effect of the direct heat to BPH tissues and circulatory disturbances which may accelerate tissue damage.View full abstractDownload PDF (4111K) -
YUTAKA TAKEZAWA, YASUHIRO SHIBATA, KEIGO OKAMURA, NOBUAKI OHTAKE, KATS ...1996 Volume 46 Issue 2 Pages 155-160
Published: March 01, 1996
Released on J-STAGE: October 15, 2009
JOURNAL FREE ACCESSWe reviewed 37 cases of acute scrotum experienced in Tone Chuo Hospital between June 1 1987 and December 31, 1994. The final diagnoses in 37 were trauma in 6, tumor in 3, acute epididymitis in 21, torsion of testicular cord in 4, torsion of appendage in 2 and acute orchitis in 1. Patient mean age were 41.4 in trauma, 30.0 in tumor, 43.8 in acute epididymitis, 10.5 intorsion of testicular cord, 8.0 in torsion of appendage and 27 in acute orchitis. The interval between onset and hospital visit were 5.8 hours in trauma, 84 hours in tumor, 72.6 hours in acute epididymitis, 7.25 hours in torsion of testicular cord, 42 hours in torsion of appendage and 24 hours in acute orchitis. The testes in 4 cases torted intravaginally and had anatomical abnormality of testis fixation. We performed orchiopexy for the affected sides and other sides, which also had an abnormality of testis fixation. We were able to preserve all testes except for 3 cases of tumor.View full abstractDownload PDF (1741K) -
SEIJI NAKATA, JIN SATO, NOBUAKI OHTAKE, HIDETOSHI YAMANAKA1996 Volume 46 Issue 2 Pages 161-167
Published: March 01, 1996
Released on J-STAGE: October 15, 2009
JOURNAL FREE ACCESSWe analyzed the details of cancer deaths in Gunma Prefecture. Age-adjusted death rates (adjusted to the 1985 model population of Japan) in Japan increased in cancers of the colon (males and females), gallbladder (males and females), kidney (males and females), liver (males), lung (males and females), prostate, breast (females) and ovary, and decreased in cancers of the esophagus (males and females), stomach (males and females), rectum (females), bladder (females) and uterus. Sites in which the degree of increase was the greatest were the colon in both males and females, and the greatest degree decrease was in stomach cancer in males and cancer of the uterus in females. Standardized mortality ratios (SMR) of each cancer in Gunma Prefecture were generally low. In all cancer deaths, the ranking of SMR in Japan were 40s in both males and females. However, cancer of the esophagus (males and females), stomach (males), gallbladder (males and females) and prostate showed comparatively high SMRs. SMRs by 10 districts in the prefecture in the main cancers showed characteristic distribution. Distribution of SMRs in males and females in the same sites were not similar. No sites without rectum and breast in females showed similar distribution of SMRs between them.View full abstractDownload PDF (1002K) -
KIYOHIRO OHSHIMA, TSUNEHIRO ISHIDA, TERUO KUSABA, SHINICHI KAKINUMA, K ...1996 Volume 46 Issue 2 Pages 169-174
Published: March 01, 1996
Released on J-STAGE: October 15, 2009
JOURNAL FREE ACCESSA full thickness chest wall resection was performed for two cases of locoregional recurrent breast carcinoma. Chest wall defects were reconstructed with a muscle flap utilizing the pectoralis major muscle in one case, and with the vertical musculocutaneous flap using a contralateral rectus abdominis muscle in the other case. Postoperative complications such as flail chest or wound infection did not occur in either case. The postoperative course of the two patients was uneventful. The quality of life appeared to be improved.
Full thickness chest wall resection with immediate reconstruction using a musculocutaneous flap is effective for localized chest wall recurrent breast carcinoma, along with multidisplinary treatment such as radiation, chemotherapy and endocrine therapy.View full abstractDownload PDF (2550K) -
1996 Volume 46 Issue 2 Pages 175-177
Published: March 01, 1996
Released on J-STAGE: October 15, 2009
JOURNAL FREE ACCESSDownload PDF (494K) -
1996 Volume 46 Issue 2 Pages 179-194
Published: March 01, 1996
Released on J-STAGE: October 15, 2009
JOURNAL FREE ACCESSDownload PDF (3547K) -
1996 Volume 46 Issue 2 Pages 195-201
Published: March 01, 1996
Released on J-STAGE: October 15, 2009
JOURNAL FREE ACCESSDownload PDF (1435K)
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