Asthma due to inhalation of powdered ingredient of Amorphophalus konjac (Konjac flying-powder) flying from factories manufacturing konnyaku (Japanese food made from the root of this plant) was designated by us “Konjac asthma”.
On 2 patients of this asthma, recently hospitalized, various tests were performed with extract of the above mentioned powder, and the following results were obtained : 1) Both patients were positive in skin test, scratch test, ocular test (conjunctival test), nose mucosa test, and Prausnitz-Küstner test. 2) Sera from them exhibited precipitation against the extract, but this can not be established as true immunological precipitation. 3) Serum from case 1 was negative in in vitrol neutralization test. 4) As the result of analysis for the allergen by skin test and others, it was estimated to be found in protein fraction of the powder extract. 5) Skin reactions and precipitations before, during and after the induction of asthmatic paroxvmum and cortisone administration were compared, without any remarkable differences.
From these results, it was considered that allergy had been established in the two patients against the extract of conjac flying-powder.

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To investigate the effect of methylthiouracil treatment of rat on protein composition of its I¹³¹ labeled thyroid tissue, paper-electrophoresis of its homogenate was performed. The results were as follows : 1) When thyroid was extracted from normal rats 24 hours after the injection of I¹³¹, almost all the radioactivity was concentrated at the origin of the paper-electrogram from its homogenate. 2) When thyroid was extracted from methylthiouraciltreated rats 24 hours after the injection of I¹³¹, the radioactivity was stronger on the spot about 35 mm from the origin toward the anode than on the origin. 3) When thyroid was extraced from normal rats 2 hours after the injection of I¹³¹, the radioactivity on the above described spot was weaker than at the origin.

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In my previous experiments, Cs¹³⁷ was subcutaneously given, in a dose of 0.1μc/0.1cc, to dd strain male mice and the effects of adrenocortical hormones, thyroxin, adrenalin, insulin and sexual hormones upon its excretion were investigated daily for 4 days and its distribution in the body at 4th day was examined. In the present experiments, various hypophyseal hormones were given to mice subcutaneously and change in the excretion and distribution of Cs¹³⁷ were investigated with the following results : 1) The administration of 0.1IU ACTH produced significant increase in the excretion of Cs¹³⁷ at the initial stage and tended to slightly accelerate it at the later period. The distribution of Cs¹³⁷ tended to be decreased in muscle, kidney and blood. When 0.5IU ACTH were given, the exeretion of Cs¹³⁷ decerased significantly at the initial stage, but no change for the late period. The disttibution of Cs¹³⁷ tended to be increased in mucsle, testis, renal and liver, but no change in other organs. 2) When GH and TSH were given, the excretion of Cs¹³⁷ was scarcely affected or slightly inhibited. The distribution of Cs¹³⁷ tended to be increased in muscle, testis, renal and liver, but no chage in other organs. 3) The administration of vasopressin was shown to inhibit the excretion of Cs¹³⁷ at the initial stage, but tended to slightly accelerate it, at the later period. The distributions of Cs¹³⁷ tended to increase in each organ.

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Growth of E. coli in different conditions was studied. The bacterium was grown in synthetic medium in a shaking flask and the results obtained were as follows : 1) Lag phase of growth was shortened and the growth rate was increased by the shaking culture. 2) In the shaking culture, increase in the weight of cells at the phase of multiplication, which corresponded to exponential phase of the static culture, were linearly proportional to time of incubation. 3) The cells transfered to a new medium from culture of multiplicating phase grew more rapidly and the lag phase was shortened. 4) Growth hormon of higher plants, supernatant of sonicated cells and other biological agents have no effect on growth pattern of the bacteria. 5) The fact that these agents on growth of E. coli under the experimental conditions were ineffective, suggests as follows :
a) These are not necessary for growth.
b) These are synthesized by the bacterium from the components of the medium. Further detailed experiments are running.

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Male albino rats, weighing about 120g, were fed cystin-deficient food, and then injected with glucuronic acid and methionine, respectively, to make comparative investigations on the effect of these chemicals chiefly on hepatic lesions, with the following results : 1) Remarkable decrease in body weight was observed in controls, but the decrease was slight in the glucuronic acid group, and it was not observed in the methionine group. 2) In the glucuronic acid group, fatty degeneration of hepatic cells was slighter than in controls, and showed remarkable decrease in it, especially from the 4th to 7th week. Further, in this group swelling and atrophy of hepatic cells were milder than in controls, glycogen level was maintained nearly normal, and no severe change was seen in mitochondria. In the 10th week, however, atrophies of considerable degree, disappearance or decrease of glycogen, and pathological change in mitochondria, which were nearly equal to pictures in controls, were observed. 3) In the methionine group, fatty degeneration was not diffuse but mainly peripheral unlike the other two groups. And the swelling, atrophy, glycogen level, and change in mitochondria were all milder than in the others. In the 10th week, severer atrophies in central parts and general decrease in glycogen level were noted, but they were far slighter than in the other two groups. 4) There facts seem to indicate that in the glucuronic acid group, the lesion of hepatic cells was inhibited by their activation, while in the methionine group, the inhibition was due to the turnover of methionine into cystine to supplement cystine-deficient food. Namely, the mechanism of the inhibition was different in the cystine and methionine group.

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Investigations were carried out on the relation of adrenal cortical hormone to stomach ulcer produced by the injection of diphtheric toxin, and on the effect of glucuronic acid on this ulccr.
1) Stomach ulcer by diphthcric toxin was made severer by the administration of hydrocortisone, and remarkably improved by the administration of glucuronic acid preparation. 2) It is considered that this ulcer has much of the features of stress-ulcer, and that the inhibition by glucuronic acid is due to the binding of adrenal cortical hormone by this acid.

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In some infections diseases, it has been known that the primary sublethal infection causes a powerful resistence to further infection by the same pathogen. In the study of experimental typhoid, it was reported that the immunization with living vaccine causes the strong and long-lasting resistance to further infection. But the immunization with killed vaccine also showed some effect on the infection of mice with S.enteritidis.
But the immune effect of killed vaccine varied with the combination of the routes of immunization and challenge. In the previous paper it was reported that intraperitoneal immunization with killed vaccine caused the local defence activity in the abdominal cavity.
In this experiment, it was also confirmed that the cutaneous immunization with killed vaccine produced the defence activity in the same locus of immunized skin. This mechanisms was discussed and histo-pathological figures of the immunized skin was described.

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From 1949 to 1950, there was an considerably severe prevalence of the rabies in Gunma prefecture. A diagnosis of the rabies was made in 58 and 119 dogs in 1949 and 1950 respectively, based on the histopathologic studies of the brain performed by pathologic department of Gunma university. The changes of the brain in rabic dogs were characterized by nerve cell degeneration. Glial nodes and perivascular cuffing being most conspicious in the surrounding nuclei of the third and fourth ventricles. The characteristic inclusions known as Negri's bodies were evidenced in the nerve cells of the hypocampal gyri in 157 of 178 rabic dogs examined. I want to consider that the metachromatic materials, which appear in the nerve cell nuclei concurrently with degeneration and destruction of the nucleoli, approach to and extricate over the nuclear membrane, play an chief role in the formation of the cytoplasmic inclusions.

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Bilateral adrenal enucleation (AE) was performed on rats, and at 24 hours, 3 days, 1, 2, 3, 4, 6, 8, 10, 15 and 20 weeks after the operation, changes in the anterior pituitary were histologically observed. Also the changes at 10 weeks after right adrenalectomy plus left AE, and the changes at 3 and 7 days after adrenalectomy were respectively compared with shamoperated controls.
After AE, enlargement and increase in number of β-cells in the wide sense were observed in the anterior pituitary. This change was most remarkable at 14 postoperative weeks, and at this period, nuclei in enlarged spherical β-cells were generally shrunken, conspicuously showing signet rings. Further, characteristic cells which showed relatively large colloidal substance and vacuolization appeared. In this case, some changes were observed also in α-and γ-cells, but these were generally slight. These changes returned nearly to the normal after 10 weeks. Findings at 10 weeks after right adrenalectomy plus left AE were approximately equal to those of AE at 10 weeks.
At 3 days after adrenalectomy, slight enlargement of β-cells was observed, whereas after 7 days, atrophy and degeneration of α-and β-cells were remarkable. This was considered to be the results of diminished reaction due to weakening of the body.
From these results, probability was estimated that ACTH would be produced by β-cells in the wide sense in the anterior pituitary.

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The author investigated the relation of filter electrophoretic fraction and the Shichijo's serum reaction of serum protein from 55 cases in later pregnancy to the body weights of their newborns at parturition. 1) The body wights of the nowborns had tendency of inverse correlation to serum albumin (relative concentration) from their respective mothers in pregnancy, and correlation to β globulin (absolute concentration), γ globulin (relative and absolute concentration) and total protein from the same. 2) The newborns from mothers who show negative or intermediate Shichijo's reaction in their pregnancy tended to be heavier than those from positive mothers. 3) Besides, the body weights of the newborns had tendency of correlation to serum albumin, γ globulin and total protein of theirself.

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With 104 middle school girls in Maebashi City, Gunma Prefecture, blood pressure was determined every other month for 3 years beginning at the age of 12 years. At the same time, somatoscopy and investigation for menarche were also carried. 1) The systolic blood pressure tended to rise each year, and this tendency was more remarkable in the urban than in the rural quarters.
2) Regional difference in the blood pressure was already observed in girls in puberty. Namery, averge systolic blood pressure for girls in rural quarters tended to be higher than those in urban quarters. This tendency was observed more distinctly when groups of nearly the same sexual maturation were compared. 3) Seasonal periodicity of the systolic blood pressure could not be seen so evidently in this age group. 4) Between the systolic blood pressure, body weight, height and sitting height, there was positibe correlation, not of a high degree, though statistically significant. When group of nearly the same sexual maturation were compared, correlation index was still smaller. 5) Temporal relation between menarche and blood pressure change (mainly rise) was intimate, the majority showing remarkable cange in the systolic blood pressure within several months around menarche. 6) Temporary rise in the systolic blood pressure due to mental tension was more remarkable in hypertensive group than in the normal. In the majority it dropped to the stable state in several minutes. 7) When the systolic blood pressure of over 130mmHg was designated as hypertension, its rate gradually rose from the age of adout 12 years, and was higher in the rural quarters than in the urban. 8) Hypertension in puberal girls was mostly transient, but there were also a few cases in whom it persisted for 23 years.

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The first group of survey consisted of 523 tuberculous patients registered in 1955 in the jurisdictions of five health centers in Gunma Prefecture, who were found tuberculous bacilli positive by sputum culture. The second consisted of 116 persons who were found to be open and active tuberculous patients about one year after collective examinations in 1956 in the jurisdiction of Shibukawa health center. Each group was divided into recipients and non-recipients of medical treatments, and they were compared with respect to biological and social conditions, with the following results : 1) With respect to sex and age, the percentage of the recipients of medical treatments was higher for women of middle ages among the previously registered patients, whereas it was in contrast, lower for the same among the patients found by the collective examinations. 2) Rentgenographic examinations revealed that the old disease type was rather more neglected than the new type. 3) There was remarkable period of indifference to the medical treatment about 35 years after the onset the disease. 4) The cause of the suspension was found more in the ignorance of the patient than living conditions.

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