For clarifing the association of acid mucopolysaccharides with transposition of the palatine shelf in mouse (ddN strain) embryos, histochemical and macroscopic observations were made on the shelf prior to horizontal transposition. The latter observation was done by removing the tongue together with the lower jaw in Tyrode solution at 37°C. The results obtained were as follows :
1) The anterior portion of the shelf becomes quickly horizontal unchanging its shape, when the tongue is removd from the embryo with still vertically lying shelf on the 14th day of gestation. The posterior portion, however, remains in the original vertical position. The same phenomena are also seen in the embryo late on the 13th day of gestation.
2) In the anterior portion, histochemically demonstrable acid mucopolysaccharides appear only just several hours before the normal transposition, which occurs near the middle of the 14th day of gestation. In the posterior, however, the same material appears in more earlier stages of the development. In either case, an increase of alkaline phosphatase precedes the appearance of acid mucopolysaccharides in the tissue.
3) The transposition of the posterior portion is achieved by bulging the medial wall with simultaneously regressing the ventral wall of the shelf. The begins early and is completed in the middle on the 14th day.
The following conclusions were drawn the above that :
1) Transposition of the shelf goes in two different ways; anteriorly by rotation and posteriorly by transformtion.
2) Acid mucopolysaccharides seem to have no intimate correlation with the transposition, anteriorly, but to play an important role, posteriorly.
3) It is interesting that appearance of alkaline phosphatase precedes alwsys that of acid mucopolysaccharides. The significance of this fact remains yet as a further problem.

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The RNA content, the DNA content, the ratio RNA/DNA and the incorporation of thymidine-C¹⁴ into the DNA were biochemically studied on the gastric cancer tissue and the gastric ulcer tissue.
1. As to RNA content there was no significant difference between the gastric cancer tissue and their control tissue or the gastric ulcer tissue. DNA content incresed in the gastric cancer tissue. Emphasis should be laid on the fact that the incorporation of thymidine-C¹⁴ into DNA in the gastric cancr tissue increased and on the contrary, in the gastric ulcer tissue decreased.
2. Administration of Mitomycin C by intra-arterial infusion to gastric cancer caused a slight decrease in the incorporation of thymidine-C¹⁴ into DNA.
The data suggest that the anticancer agent (Mitomycin C) produces inhibiting effect on the DNA metabolism in gastric cancer tissue.

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During the years 1959 to 1966, about 10, 500 sera were collected in Gunma Prefecture and antibody to Japanese encephalitis virus was measured by both hemaggultination-inhibition and neutralization tests. The neutralization test employed for this study was the recently described plaque reduction method of Oya.
From the data of the distribution of antibody to Japanese encephalitis virus in various age groups in 3 geographically different areas, the effect of antibody patterns in human populations on attack rate was suggested. The method of the neutralization test was most sensitive for detection of anitbody and it seemed to be an effective laboratory tool for serum survey because of the ease and inexpense of performance.

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Recently a number of refined techniques such as X-ray examination, endoscopic study, and biopsy under direct observation are available for the diagnosis of early gastric cancer.
Althongh the combination or either one of those techniques is essential for the diagnosis of early carly cancer, it is not possible to use such techniques in a social wide screening and a more simple technique is desired at the preaent time. Our present attempt was thus forcuseb to find out which one of laboratory tests commonly used coulb be used for the substituatin of such refined techniques.
Laboratory tests used here were Shichi jos reaction, acidity in the stomach juice, anemia, decrease of body weight, occult blood in the feces, red blood cell sedimention rate, and subjective complaints.
Analysis of these laboratory findings in combination with above mentioned refinined techniques has indicated that anacidity and positive reaction of Shichijos reaction are relatively reliable tools for suspecting early cancer of the stomach.
Abnormalities in other 5 laboratory tests, however, have no relation to the occurrence of early stomach cancer.
It is suggested that abnormality in Shichijo s reaction and anacidity might de highly suggestve findings for detecting early cancer of the stomach.

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The pulmonary ventilatory function were studied in 292 healthy men inhabiting Gunma Prefecture (with range of 20 to 61 age) with the use of Benedict-Roth 13.5 L type, anb normal values were estimated. The results were followings.
1) Predicton formulae of vital capacity (VC) is :
VC (Standing) = (28.61-0.110×Age) ×Height (Age is in years : Height in cm)
2) Prediction formulae of maximal breathing capacity (MBC) is :
MBC= (92.76-0.391 × Age) × B. S. A. (B. S. A. -Boby surface area in m²)
3) Prediction formulae of one second forced expiratory volume (FEV 1.0) is :
FEV1.0= (26.59-0.16×Age) ×Height (Age is in years : Height in cm)
In addition, the correlation coeficient of pulmonary function measurements to physical characteristics were calculated in the same subjects.
The summary were followings.
The vital capacity were correlated well with height and age. There were a high negative correlation of vital capacity with age (r=-0.690).
Maximal breathing capacity were correlated well with height (r=0.401), but significant correlation between MBC and body surface area were not obtaind in our study (r=0.163).

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