Previous stud : es have demonstrated that osteomyelitic staphylococci readily provoke multiple abscesses in long bones of young rabbits to a significant degree compared to other strains and produce coagulase most abundantly at pH. 8.3-7.7 in 90% of these strains, whereas other strains do so at pH. 6.7-7 0. In this papers it has been studied as followings :
1). strains purely cultivated from 14 cases of acute osteomyelitis produce coagulase with optimum at pH. 8.0-7.7, including exceptional 2 strains from which such sub-cultures have been selectively obtained, and 16 strains cultivated from the other infections and 2 strains selected from the subcultures of the osteomyelitic 2 strains do so at pH. 7.4-7.0. The isoelectric point of the coagulases originated from osteomyelitic strains is at pH. 2.8-2. 5 culculated by acid precipitations distinctly from the others being at pH. 4.0-3.8. So purified coagulasea have been harvested most abundantly by the aids of these optimal production and isoelectric point, showing 672 to 465 fold purer than the crude materials as culculated on the basis of activities to a mg. of protain concentrations.
2). Purified coagulases originated from osteomyelitic strains and the other strains are clearly differentiated in each others by zone electrophoresis with starch as supporting media, and especially separated in phosphate buffer at pH. 6. 8. The electrophoretically separated coagulases have shown 2 to 3 fold purer than the ones by acid precipitations.
3). Anticoagulases recovered homologously, to osteomyelitic coagulases or the others in each immunitized rabbits. The patients of osteomyelitis and the other infections, although small cases, have shown specific immunological responces in the neutralisation of the coagulases originated from osteomyelitic strains and the others.
In 1948 Tager found two mobil components in electrophoresis of coagulases, in 1950 Rammelkamp discovered three types of coagulases which produced specific anticoagulase and in 1958 Barber found specific anticoagulase related to phage groups, but had no attentions to the differences in the infectious diseases.

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Cinchophen was administered to dogs either orally or intravenously in a dose of 0.15 g/kg for about 3 weeks, and the development of typical gastric ulcer was observed in all the cases. At the same time, activation was demonstrated in neural secretion of the hypothalamus and in function of the adrenal cortex. In total pouch dogs, the secretion of gastric juice began about 3 hours after the oral administration of cinchophen, and lasted about 3 hours. In this case, the secretion of hydrochloric acid, especially pepsin, was remarkably accelerated, and different secretion curve from those of insulin and histamine was obtained.
When the pituitary stalk was completely cut, the flow of neural secretion after cinchophen administration was checked at the section of the nerve bundles, and neither adrenocortical function, nor gastric juice secretion was activated, and the development of gastric ulcer was completely inhibited. In dogs whose vagus and sympathetic nerve were blocked, adrenocortical hyperfunction took place after cinchophen administration samely as in normal dogs. Also characteristic enhanced secretion of gastric juice and development of typical gastric ulcer were observed.
In bilateral adrenalectomized dogs, gastric juice was not eahanced by cinchophen administration, but typical gastric ulcer developed. This latter was also observed either in bilateral testectomy or bilateral thyroidectomy, both combined with adrenalectomy.

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Histological change in gastric glandular cells after the administration of chinchophen was comparatively investigated with that induced by insulin and histamine, and findings were obtained indicating the acting mechanism of chinchophen.
1. In 3-4 hours after chinchophen administration, the function of the chief cells was activated to synthesize secretory granules, and at 6 hours their release was observed. Also in the parietal cells, change was seen at 3-6 hours, but not so evident as that after histamine administration.
2. Release of secretory granules induced by insulin was inhibited by vagotomy, but that by chinchophen was observed in vagotomized animals as well as in intact ones.
3. In adrenalectomized animals, remarkable involution of both cells, especially of the chief cell, was present, and hyperfunctional picture could not be seen after the administration of chinchophen.
4. In hypophysectomized animals, both cells did not show secretory activation after chinchophen administration.
5. Anticholinergic drugs inhibited the release of the secretory granules induced by chinchophen as well as by insulin, but not so remarkably inhibited, induced by histamine.
From these findings, it can be assumed that chinchophen would exert its pepsin secretory action humorally through the hypophysio-adrenal system, and that adrenal hormone would act directly to the gastric chief cell.

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1) Mice were infected intravenously with sublethal dose of virulent Staphylococci, and the viable number of Staphylococci in the organs were investigated during the course of infection.
2) During first 3-6 hours after the injection, microorganisms were cleared from the circulatory blood by R. E. S. organs, and their viable counts in the kidneys and blood decreased.
After initial clearance stadium, microorganisms in the kidney and bone marrow began to increase with logarithmically constant velocity and the body weight of mice decreased.
3) The maximum number in the kidneys and femur &tibia were 10⁹per gram and 10⁵ per femur& tidia respectively. The tissue defence reaction was activated, and no more increase of orgamisms was observed in these organs.
4) No significant difference were found among various strains derived from human lesions on infectious pattern of liver, kidney, bone marrow and blood.

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The gastric secretion was studied in dogs with Heidenhain gastric pouch by administration. of hypothalamic lipoid extract. An intramuscular injection of the extract to intact dogs produced a significant increase in gastric acidity and pepsin value after 3 hours, reaching a maximum at 6 hours and then gradually declined to control level. This gastric secretory response was closely similar to that following intramuscular injection of ACTH. The gastric secretory response to the extract was perfectly inhibited by pituitary stalk section, hypophysectomy and adrenalectomy. While the gastric secretory response to ACTH was inhibited by adrenalectomy, but was not inhibited by pituitary stalk section and hypophysectomy. Paper-chromatographically it was revealed that the hypothalamic lipoid extract was different substance from ACTH in chemical composition. It can consequently be assumed that the gastric secretion was induced humorally by the extract which stimulate the adenohypophysis to re-lease ACTH giving an effect on the adrenal cortex. The effective factor of this extract was found in lipid portion of the hypothalamus, but was not found in extract from the cerebral. cortex.

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For the past 6 years we have cultivated 322 strains isolated from cases of trichophytia in our clinic. Microsporon japonicum was isolated from all of 35 cases with trichophytia superficialis capillitii, Trichophyton asteroides from 4 with kezion celsi, and 236 strains of Tr. purpureum, 22 strains of Tr. asteroides, 3 strains of Mic. japonicum and each 11 strains of Tr. interdigitale and Epidermophyton inguinale from several other clinical types of trichophytia superficialis.
The comparison of these with the results obtained during the preceding 9 years revealed a noteworthy fact that the number of cases of Tr. purpureum and Ep. inguinale increased.

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Recently we have treated 5 cases of remarkable ptosis of gallbladder. Moreover, 7 cases in whom the lower edge of the gallbladder reached the 4th lumbar vertebra complained of the same symptoms with the former. Below are given reports on these 12 cases.
The majority of these cases had also the ptosis of other visceral organs such as stomach, liver, kidney, etc. with tendency of body weight loss and low blood pressure. Symptoms, clinical course and results of laboratory examinations resembled those of dyskinesia of the bile duct, and therapy for dyskinesia had effect on many cases of this disease. It was consequently assumed that ptosis of gallbladder and dyskinesia of bile duct would have intimate relation, and that the former would easily be complicated with the latter. In the treatment of this disease, not only local therapy for the bile duct, but also systemic one inclusive of psychotherapy is considered necessary.

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Gudernatsch (1912) for the first time elucidated that thyroid substance acceleretes the metamorphosis of amphibia. Numerous works on the metamorphosis have been published. However, mechanism of the so-called metamorphotic phenomena, seen in mouth, muscle, respiratory organ, digestive tract, skin, tail, etc. during spontaneous metamorphosis or artificial one induced by the administration of thyroid substance still remains in perfect obscurity.
To obtaine the clue of the problem, we carried out investigations with larvae of Rana catesbiana on change in Cu⁺⁺⁺ and Zn⁺⁺ which are considered to have important bearing on physiological activity of tissue or cells, and muscular structure of the tail in stages of the tadpole and metamorphosis was examined polarographically and histochemically. As the sign of metamorphosis, the tail absorption, the most conspicuous metamorphotic phenomenon, was uesd. In the tadpole stage, a large, amount of Zn was found in the vascular wall, subcutaneous tissue, and muscle, while Cu was scarce. On the contrary, in the metamorphotic stage, a large amount of Cu was found in the muscle and Zn was relatively decreased. These were confirmed quantitatively by the results of polarography.

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Into the water in which larvae of amphibia were bred, 2.05μC/cc of I¹³¹ or 1.70μC/cc of P³² was placed, and change in histological picture of the thyroid was observed consecutively.
1) The course of change ill the thyroid gland was as follows : In the first place, cytoplasm of epithelial cells became granular or vacuolated, followed by atrophy of nuclei, and finally follicular structure was destroyed. In this stage, connective tissue, that is, stroma, in the periphery of the gland began to be proliferated, and it gradually spread towards the center.
2) The effect of isotope was not uniform on all the follicles, and degenerations of mild, medium and high degree were mixed.
3) Upon the thyroid, effect of P³² was generally milder than that of I¹³¹.

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Effects of thyroid substance upon morphological development : The administration of thyroid substance accelerated metamorphosis, but morphological disharmony was present. Namely, imperfect formation and premature involution of larval organs and premature development of adult organs with their imperfect formations were observed. If the development of the larva of Bufo vulgaris formosus is divided into 4 stages of early larva, tadpole, premetamorphosis, and metamorphosis, artificial acceleration brought about remarkable shortening, of the tadpole stage, anb prolongation of the premetamorphosis stage.
Effects on mesodermal tissue : The effect of thyroid substance earliest appeared on Mesenchymal tissue. The formation of cartilage and muscle was accelerated, and enlargement of pronephros, swelling of pronephric tubular epithelium, and scattered subcutaneous hemorrhages were observed.

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Effects on entodermal tissue : Thyroid gland showed atrophy as the result of the administration of thyroid substance. The thymus was enlarged, and thymocytes were increased.
Intestinal epithelium remarkably developed, but differentiation of muscularlayer was poor. Blood cells filling space between epithelial cell cords were decreased, and epithelial cells assumed large sizes, containing a large amount of light cytoplasm. Internal gill was undergoing rapid involution, but the formation of pulmonary alveoli showed a picture of the early stage, no differentiation of smooth muscle being observed.
Effects on ectodermal tissue : Remnants of yolk granules were visible in the brain tissue, the degree of differentiation being low. Active mitoses could be recognized in the ependymal layer. In the adenohypophysis, hardly was observabla any remarkable change, but in the neurohypophysis, enlargement of cells and decrease in colloid were seen.
If changes in tissue in metamorphosis is classified into decomposition and formation phases, disharmony was present between these two phases. For examples, against the involution of the internal gill, the development of the lung was delayed, and against the development of intestinal epithelium that of muscular layer, that is, its ground work, was behind the normal. In this way, disharmony was observed, in accelerated metamorphosis, between involution and formation of the organs as well as in morphology. And when decomposition, that is, the degeneration and disappearance of larval organs was compared with assimilation, that is, the formation of adult organs, the former seemed to precede.

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A small sample study of comparative measurement for cardiac enlargement by means of direct roentgenography and fluorography was presented. The purpose of the study was to determine the possibility of an application of fluorography in the field work of heart disease, control program. As the conclusion, there was a significant difference of variance among the direct roentgenographic, fluorographic measurements of 60×60mm and 35mm miniature size. However, no significant difference was found among various diameter ratio in three groups, while a slight measurement error for particular diameter should be expected. In addition, comparative observation on diameter ratio between cardiac patients and healthy subjects revealed that the former has larger ratios in which contain Lb, or Mr as a denominator, than the latter.

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Fluographical measurement of heart size for 569 apparently healthy adult subjects was undertaken. The method of study was identical with the previous one. The measurements obtained were analyzed for age, sex, familial tendency, and blood pressure determinations. Distribution of various diameter ratios was presented and discussed from related findings such as, incidence of hypertensives and of heart enlargement.
The summarized findings are as follows :
1.Sex difference in normal range of certain diameter ratios should be considered.
2.Most diameter ratios were positively correlated with age but for oQ/uQ, oQ/Br which were negatively correlated.
3.Judging from the incidence of hypertensive, uQ/Br, oQ/uQ, Mr/Lb were significantly associated with the incidence.
4.The incidence of heart enlargement based on Mr/Lb, or Tr/Lb revealed a close association with three groups of blood pressure level.
As conclusion, an additional use of certain diameter ratios such as uQ/Br and oQ/uQ were considered to have a significance for detecticn of cardiac abnormalities.

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An enzyme system in Proteus Concerning with reduction of thiosulfate by formate was divided into five components ; formic dehydrogenase, a particulate hydrogen carrier, an anaerobic factor, dialyzable factor (s) and terminal reductase of thiosulfate.
It was observed that each of them have different sensitivity to enzymic inhibitors and sonic oscillation. They are essential for thiosulfate reduction.
The anaerobic factor which was produced only by anaerobically grown cells was purified. The relative activity of this factor iucreased to 45 times of the crude extract.
Relatiou between biological oxidation reduction system and formation of the anaerobic factor was discussed.

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With the object to study the course of healing of surgical wound and the effect of various drugs on it, wounds in rabbits were pathohistologically observed for 24 hours to 5 months after operation with the following results :
Drugs used in the present experiments were the following six, all of which were locally applied to sutured site.
1. A 10, 000-fold nitrogen mustard solution, intradermally injected in a dose of 0. 1cc.
2. About 0.1g of 1% hydrocortisone ointment, applied once daily for 10 consecutive days.
3. A 10-fold dilution of Typhogelison, intradermally injected in a dose of 0. 1cc.
4. About 0.1g of Hirudoid ointment applied once daily for 10 consecutive days.
5. A 10, 000-fold Quinhydrone solution (containing 3% carboxymethylcellulose) externally applied.
6. A 5, 000-fold Quinhydrone solution, externally applied.
Results were as follows :
1. In the healing course of sutured wouhd of the rabbit, restoration of the epidermis preceded that of the connective tissue, and regenerated epidermis turned internally, iuvading the wound.
The restoration of connective tissue was performed mainly by the upward proliferation of lower reticular layer of the corium, and muscular layer, which play a main role in fibroplasia. In the reticular layer, all tissue responses were weak through the whole course of healing.
Elastic fibers showed a sign of regeneration in 3 to 4 days, and on the 7th day, obvions regeneration was observed in myoblastic tissue.
There was no evident increase in PAS-positive substance, and it was scarce in regenerated collagenons fibers.
2. After the intradeimal injection of 0.1cc of a 10, 000-foId nitrogen mustard solntion, and of 0.1cc of a 10-fold Typhogelison solution, and external application of a 10, 000-fold Quinhydrone solution, exudative response was not elicited in any case. And the formation of myoblasts was slight, fibroplasia rapid, without accompaniment of the degeneration of fibroblasts, regeneration of epidermis accelerated, and shrinkage of cicatrix effected.
After external application of a 1% hydrocortisone ointment and a 5, 000-fold Quinhydrone solution, there were degeneration and destruction of fibrils, shrinkage of their nuclei, poor formation of myoblasts accompanied by vacuolation, and delayed fibroplasia, indicating imperfect healing. These treatments are therefore ineffective for surgical wound.
The application of Hirudoid ointment produced remarkable delay in fibroplasia, and has unsatisfactory effect, when used immediately after operation.
The effect of these drugs on elastic fibers and P AS-positive substance was not so remarkable. And the regeneration of elastic fibers tended to delay and PAS-positive substance tended to decrease in proportion with the degree of tissue injury.

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Immunical studies on tapeworm disease were conducted and the following results were obtained.
1) Resistance against reinfection of Sparganum mansoni has been observed in the dogs previously infected with it.
2) In the serum of the rabbits, immunized with antigen obtained from Sparganum mansoni by means of Sonic disintegration, the titer of precipitation raised parallel to increment of γ-globulin, but the latter decreased earlier than the former.
3) Significant difference between Sparganum mansoni and Diphyllobothrium mansoni was verified immunologically.
4) The dogs immunized with antigen prepared from Sparganum mansoni showed the resistance to infection.
5) Zone electrophoretic studies on antigens prepared from Sparganum mansoni were made.

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Three kinds of ointments such as Grave Salbe Thimenosal and Mercurocrom Salbe were used in a Preliminary Screening Test in an attempt to determine which ointments warranted further testing as protective agents against the penetration of cercariae of S. japonicum. Of these 3 preparations Timenosal in the Hg dilution of 1 : 500 gave 90-95 “Per Cent Relative Protection” for mice for 30 mimutes against the cercariae of S. japonicum.
The experiments with the other 2 preparations in every dilution were disappointing.

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Of 23 patien who were treated orally with a total dosage of 1.200 mg of dithiazanine
(daily 400 mg for fhree days) 9 patients (39.1%) were cured.
Of 11 patients treated with a total dosage of 2, 000 mg (daily 400mg for five days) 5 paients (45.5%) were cured.
when a total dosage of 1, 800mg (daily 600mg for three days) were administered in 13 patients, 8, patients (69.2%) were cured.
With th administration of dithiazanine the side toxic reactions have been observed in 50% of the patients, but their symptoms were not severe and subsided within 24 hours

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Immunization of animals with adultworm-and egg-antigen and passive immunization with antiserum were performed. With these animals, precipitin tests, change of serum protein and protection against reinfection were studied.
I. protection against infection of S. japonicum was obtained in mice injected with adultworms-antigen but no protection was observed in mice immunized with eggs-antigen or immunized passively with antiserum.
II. 2 antigens, prepared from adult-worms and eggs, gave positive results in precipitin test using immune sera of rabbits, immunized with adultworms-, eggs-antigen or immunized passively with antiserum.
The highest precipitin titers of rabbits sera immunized with adultworm were 3, 200 X against adult test-antigen and 100 X against egg test-antigen. Sera from rabbits immunized with egg-antigen showed the highest precipitin titers of 800 X against adult test-antigen and 6, 400 X against egg test-antigen. Rabbits sera, immunized passively with antiserum of goats, gave positive reaction at 6, 400 fold dilution to adult or egg test-antigen.
III. The change of serum protein in mice injected with suspension of adult-worms and eggs and antiserum from immunized goats was investigated by paper-electrophoresis. The serum protein of mice injected with suspension of adults showed remarkable increase of total protein slight increase in α-, β-globulin, increase in γ-globulin and remarkable decrease in A/G ratio. The serum protein of mice injected with suspension of eggs showed remarkable increase of total protein, slight increase in β-, γ-globulin and decrease in A/G ratio. Increase of total protein, slight increase in albumin and γ-globulin were observed in mice immunized passively with antiserum.

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About the method of inspecting Hookworm carrier, stool examinations such as smear, floatation, centrifugation and culture method have been employed; but the stool examination of many people could not be easily performed becanse of its much labour. Present studies were carried out for the purpose of diagnosis of Ancylostomiasis by means of immunological method.
Crude antigen were isolated from dried adult worms of Ancylostoma caninum, by grinding in a mortar adding with phosphate buffer and Sonic disintegration, and fractionated by graded saturation of Ammonium Sulphate.
The serum of immunized rabbit showed a positive precipitin reaction with crude antigen in the dilution of 1 : 25, 600 and the serum of infected dog showed a positive reaction with the same antigen in the dilution of 1 : 1, 600 and the serum of hookworm carrier save a positive reaction in the dilution ol 3, 200 to 1 : 6, 400.
Intradermal tests were conducted in dogs.and individuals infectd with hookworm, using varions antigens. The antigen, prepared by precipitation with 60-70% Amm. Sulphate suturation, gave the most noticeable reaction of the infected dogs.
In persons, the reaction reached at maximum by 20 min. The reaction was determined positive positive when the size of wheal was above 10 mm.
Of 55 hookworm carriers, 49 cases gave positive reactions (89.1%), showing a remarkable wheal accompanied with redness, to the same antigen.
Of 23 control peaple who were negative in stool examination, 6 cases gave positive reaction (26.1%).

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