Recent entomological studies carried out in Sakai City, Osaka, Japan showed a high density of
Culex pipiens complex. We performed PCR-based molecular identification of
Cx. pipiens complex to show local distribution of the members of
Cx. pipiens complex and variation of the species composition in Sakai City. CDC miniature suction traps without a lamp enhanced with 1 kg of dry ice were used to collect adult mosquitoes at 12 collection sites from April 2009 to March 2010. Among 1,591
Cx. pipiens complex collected in this study, 1,044 (65.6%) were identified as
Cx. p. pallens, 474 (29.8%) were
Cx. p. form
molestus, 62 were hybrids between
Cx. p. pallens and
Cx. p. form
molestus, and 3 were identified as
Cx. quinquefasciatus.
Culex p. pallens were not collected in winter from December 2009 to March 2010.
Culex p. form
molestus were not collected in January to March 2010. Hybrids between
Cx. p. pallens and
Cx. p. form
molestus were not collected in December 2009 and January 2010.
Culex quinquefasciatus was collected at 3 different collection sites in summer from July to September 2009. The
Cx. pipiens complex composition was significantly different among 3 different categories of collection site.
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