著者は, 前の報告で, Pullets 及び産卵鶏の前舌腺の腺細胞が核の中に, グリコーゲンを含有することを報告した. ひきつづいて, 今回, 雄鶏の前舌腺にも, 同じようなことが見られるかどうかをしらべた.
材料は, 2日令から180日令の白色レグホーン28羽及び180日令の横斑プリマウスロック6羽であった. Carnoy 固定, パラフィン包埋, PAS染色. グリコーゲンの確認は, 唾液, diastase, α-amylase 及びβ-amylase の消化試験によった.
成績は次の通りであった.
1. 7羽の100日令の cockerels のうち, 4羽の前舌腺の腺細胞にグリコーゲン含有核が発現した. その頻度は, 1.5-21.5%, 平均8.0%であった. 180日令の cockerels, 60日令以下の cockerels 及び豆雄ヒナには見られなかった.
2. グリコーゲン含有核を有する前舌腺は, その構造が複雑であり, 腺細胞には粘液が少なかった. 然るに, グリコーゲン含有核を有しない前舌腺は, その構造が簡単であり, 腺細胞には粘液が多かった.
3. グリコーゲン含有核の発現と, 腺の構造の変化と, 性腺の発育及びこれに関係ある hormone との間に, 関係があるように思われた. また, 一方, 雌鶏にグリコーゲン含有核が発現したと同じ機作が, ある一定時期 (たぶん100日令前後) の雄鶏にもおこるであろうことが想像された.

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人と豚の回虫を用いて回虫角皮および線維層の構造と機能の相互関係を検討した. 著者は先ず比較解剖学的ならびに機能的な見地から先人の分類を参考にして回虫体壁を次のように分類し, 各層をそれぞれ膜片位相法を主とし, 縦切切片を従として観察し, 更に組織化学的検索も併せ行った.
I. Cuticle.
1. Cortical layer: a. coating membrane, b. lenticular zone, c. basket-pattern fibrous layer.
2. Homogeneous layer.
II. Compact fibrous layer.
III. Loose fibrous layer.
IV. Muscle layer
虫体最外側に位置する coating membrane には sulfhydril 基と disulphide 基を証明でき, Chitwood の云うように特殊な keratin であると考えられる. Lenticular zone (佐藤と朝倉) の周辺の微細な線維は Mallory 染色の Cason 変法によって青染し, 膜片標本で観察すると, 輪状帯を互いにからげた竹籠様の配列が見える. 次いでこれらの線維構造物は集束下降して homogenous layer を貫き, compact fibrous layer の上部に至るが, この形状を著者は vector 図型として示し, これが体表の虫体内部に対する安定性を助ける役割を演じていることを明かにした. Homogeneous layer は各種染色に難染である. 水平 (1950) はさきに一般支持組織線維の配列が機能的適応を示すことを両棲類, 魚類等の膜片標本で観察したが, 回虫の線維についても全く同様のことが云える. 即ち compact fibrous layer を同一の方法で検討すると, 線維は互いに約140°, 体軸に対して約70°で交叉していることが判る. 尚, これらの線維は collagen と考えられるが, azan で赤染する.
回虫が宿主の細小管腔を迷入或は脱出する際には虫体が細長化するものとの仮定のもとに, 虫体を伸長して得た材料で線維交叉角を計ると, 交叉角は伸長前に比べ正弦比を以って減じていることが知れる. 更に虫体を円筒とみなし, 線維が直線状に走ると仮定した場合, ならびに線維がラセン状走行を示すものと仮定した場合の各々について最大伸長の度合を計算した結果, 長さで約3倍, 太さで約0.6倍の値を得た. 以上, 回虫体壁の構築に関して特に coating membrane の化学的性状, 各層に認められる線維構造, 殊に compact fibrous layer の特色ある線維配列形式について検索を試みたが, これらはすべて虫体の運動によって生ずる体内外の力学的張力に対しそれぞれ合目的なものであると考えられた.

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The fresh-water fish, Aryzias latipes, was bred in two pools at temperature of 15°C and 30°C for 3, 10, 25 and 30 days, and the changes of thier liver cells were observed with the electron microscope.
1. The liver cells of the fish bred in the pool of 15°C have two parts: the darker parts of the higher electron density occupying a large part of the cell, and the clearer parts of the lower electron density. The liver cells of the fish bred in the pool of 30°C have only clearer parts.
2. The liver cells of the fish in the pool of 15°C contains little lipid granules and those in the pool of 30°C show various stages of accumulation of the lipid granules.
3. The cell substances not dissolved after the treatments of BOUIN's fluid or 5% K₂Cr₂O₇ are contained much more in the liver of the 30°C-fish. It is supposed that the substances which are related to proteins have been much more synthesized.
4. The liver cells of the 30°C-fish contain besides clear mitochondria many dark mitochondria and microbodies which are supposed to be related to the lipid contents. Also the endoplasmic reticula in alveolate and vacuolate form to which small granules of PALADE are attached are seen in almost all parts of the cell.
5. It is supposed that the tendency of growing clear in the liver cells at 30°C is caused by the increase of their surface-permeability and water contents, which is based on the acceleration of metabolism inside of the cell accompanying changes of its structure. On the clear parts of the liver cells of the 15°C-fish the small granules of PALADE and mitochondria are very few.
6. The reason why these dark parts in the liver cells of the fish bred in the pool of 15°C make their appcarance is not clear. It may be related to the water contents of the cells or may be caused by the qualitative difference of the constructive substances.

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For the lymph-node tissue culture, most works were carried out by cover-slip method with classic media and culture technics up to this time. Therefore, we have studied human lymph-node by means of clinical tissue culture as well as fluorochrominized tissue culture devised in our laboratory, and also applied these technics to clinic.
Method: 1. Clinical tissue culture. After a drop of serum was spread out on a HIRAKI's tissue culture plate (depth, 200μ), the tissue fragment is placed in the center of serum and a drop of V. B₁₂ (100γ/cc) was added. A cover slip was laid on the plate and sealed with paraffin, then, the plate was placed in a incubator.
2. Fluorochrominized tissue culture. In the medium composed of a drop of serum and a drop of Ao. -V. B₁₂ solution (Acridine orange 0.2mg/cc, V. B₁₂ 80γ/cc) the tissue frament was cultured, and observed under the fluorescence microscope with OSRAM HBO 200 mercury vapor lamp, exciter filter BG 12 and barrier filter OG 5.
Result: 1. Clinical tissue culture. The growth zone of non-pathologic lymph-node is densely packed with cells in the central part, but in the intermediate to the peripheral zones less densely. In lymphadenitis simplex, the cell growth was far superior to the non-pathologic one, while in the patients with reticulosarcoma, tuberculosis, HODGKIN's disease and cancer metastasis the cell growth were mostly poor. In acute lymphocytic leukemia, cells in the growth zone were extremely dense and the boundary is distinctly defined.
2. Fluorochrominized tissue culture.
The growth zone of non-pathologic lymph-node presented diffusely yellowish green color sparsely intermingled with reddish orange fluorescent spots. As to the pathologic lymph-node with poor cell growth, reticulosarcoma presented yellowish green fluorescence but in HODGKIN's disease fluorescence had more reddish hue, and in cancer metastasis reddish orange fluorescence was found in the explant. In acute lymphocytic leukemia, fluorescence was green in the central part of the growth zone and yellowish green at the periphery.
Conclusion: Since there were some definite differences in growth pattern and fluorescence of the respective growth zone of lymph-node tissue culture in various diseases, these culture methods are useful for the diagnosis of the disease with enlarged lymph-node.

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The fresh-water fish, Aryzias latipes was bred at a suitable temperature of 25°C and at a higher temperature of 34°C for 3-47 days. Feeding and other conditions were the same as in the preceding experiment.
1. At 25°C, the electron microscope figure of the liver cells is similar to that 15°C
2. Around at 30°C, the metabolism in the liver seems to be abnormally accelerated, but when it goes up over 30°C the function fall is observed on the contrary, because of the lowering of the water contents or of pH.
3. At the condition of starvation the liver cells become darker, which is supposed to represent a stiffening of them. Also there appear large abnormal granules which seem to have grown from varients of mitochondria and microbodies.

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家鶏卵を39±0.50°Cで孵卵して, 卵黄嚢造血領域の初期血島, 及び血流開始直後の卵黄嚢血管からの血液を材料にとった. Giemsa 染色, 及び May-Grünwald-Giemsa 染色で赤血球系細胞の発達段階を観察しながら, 一方では石英スライドグラスに材料を塗抹した. 塗抹した材料は24時間ホルマリン蒸気を作用して, ヘモグロビン (以下Hbと略記) をメト-Hbとなし, メタノール固定後408mμの単色光で Heme のを顕微分光写真法で撮影した. 更に, Ralph 法でHbを染色した.
所見は次のようであった.
1. Primitive blood cell には Heme の吸光とHbの染出が認められない.
2. Promegaloblast では全細胞質に亘り, Heme の吸光が有意義の程度に均一に認められるが, basophilic megaloblast 期になって核内に現れるその顆粒状の吸光像は本期の細胞ではまだ認められない. Hbの染色像も同じ結果を示す. 故に, Hbは初期から主として細胞質中で形成されるのであろう.
3. Basophilic megaloblast の細胞質内 Heme の吸光は前者より著しく増加する. 同時に, 本期の細胞に於いて, 初めて, 顆粒状の Heme の吸光が核内に散在して現れる. 然し核内の1乃至2ケの円形部位には吸光が認められない. 恐くその位置に核小体があるのであろう. Hbの染色標本も同じ結果を示す.
4. 有糸分裂の中期から終期にある basophilic Megaloblast の Heme 吸光像では染色体が直接細胞質内にあるにも拘らず, 染色体の位置に静止核に見られるのと同じ様な顆粒状の, その他の細胞質の部分の吸光度よりいくらか強い Heme の吸光像がある. 故に, この所見と3及び4の所見から核内のHbは細胞分裂時に染色体に附着して取り込まれたものと推定せられた.

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