1) The viscosity of the intrapleural blood and effusion after resection of the lung was measured using Hess-Erma's Viscosimeter.
2) Aspirating the viscous liquid in the pleural cavity is to be performed with “the Law of Poiseuille”.
3) The length of radius of drainpipe influences mostly on the aspiration. The appropriate length of the inside diameter is from 0.7 cm to 0.9 cm.
4) The drainpipe must to be seamless from the pleural cavity to the bed and as short as possible, and the shape of its tip must to be simple.
5) So long as the air leakage from the lung continues, “Y” type tube is not adequate.
6) The appropriate number of the side holes is one or two and the appropriate position sits within 3 cm from the tip.
7) The appropriate pressure of the aspiration is from -10 to -15 cm H₂O.
8) “Three bottle catheter suction drainage consisting of a trap bottle, water seal and valve bottle in series” is favourable for the aspiration apparatus.
9) The air leakage continues for about 10 hours at soonest. The air leakage stops in 15 hours to 50 hours mostly, but not a few continue for more than 65 hours.

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As a pipe to drain blood which accumlated in the thoracic cavity after resection of the lung, a tube of Dow Couning Silastic 50 & 80 (0.8 cm in inside diameter, 1.5 cm in outside diameter and 90 cm in length) was made for trial. And the toxicity of Silicone rubber was examined and compared with that of Nelaton's rubber and vinyl.
1) Forty-eight to seventy-two hours after the resection the drainpipes were removed and tissues which were found at the tips of them were examined.
2) Silicone rubber was implanted under the fascia of 12 dogs. The local histological changes were examined after 2 to 4 weeks, and compared those of Nelaton's rubber and vinyl.
3) They were respectively implanted in the thoracic cavities of 15 dogs. After 3 to 4 weeks the cavities were reopened and the findings observed. All examination stated above revealed the toxicity and irritating action of vinyl and Nelaton's rubber were strong while Silicone rubber had no toxicity at all. The Silicone rubber drainpipe was clinically applied to about 100 cases. The result was exellent and no occlusion by clotted was experienced by the time the drainpipe was drawn out 72 hours after operation. A statistical comparison of chest X-ray films which were taken 7 days after operation disclosed that better results could be obtained with Silicone rubber drainpipe than with Nelaton's catheter and stomach drainpipe.

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Studies upon the seminal fructose, as the chief subject, have been carried out in male infertility with their clinical signs and the experiments on man and rats have also been carried out to pursue the effects upon the vasectomy.
1. Of 153 male infertility, 50.3% was azoospermia, 12.4% was oligozoospermia and 3.9% was necrozoospermia. The abnormal objective signs were observed in testis. epididymis, prostate and seminal vesicles. 64.9% were observed in azoospermia, 26.3% in oligozoospermia and 50.0% in necrozoospermia.
2. Epididymitis tuberculosa (3.9%) and epididymitis gonorrhea (30.5%) were observed in past history and inflammation of epididymis seems to have an important effect upon the secretory function of male accessory organs.
3. More detailed examinations have been carried out in 16 patients on oligozoospermia and azoospermia. The Volme of semen, seminal fructose, testicular biopsy and vesiculograms were examined and found no relation between the values of seminal fructose and the atrophy of seminiferous tubulus, whereas the most intimate connections were observed upon the development of the seminal vesicles.
4. Various values of the examinations on the volume of semen and the concentration of seminal fructose were observed in patients with urogenital diseases. Upon the tuberculoses of seminal vesicles, the values were found in high and on the cysts of seminal vesicles were found in low.
5. Upon the effects of vasectomy on normal man, the values of seminal fructose were decrease after a week period of operation and radually return to normal, whereas in rats and rabbits, the values were found unstable after the operation, but, in general, these were disposed to decrease.
6. From these above point of views, the seminal fructose on male infertility and urogenital disease seems to be controlled by the state of the seminal vesicles, and the male sexual hormones seem to have the close relation upon the secretory function.

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Although the formation of seminal fructose is carried out by the male accesory organs, the mechanism of its production is still unknown. The author carried out the experiments to determine the effects of sexual hormones upon fructose in semen using rats by weighing the seminal vesicles and testis.
1. The weights of the seminal vesicles of normal rats were ranging from 9 to 125 mg, whereas the values of fructose were ranging from 114 to 475 mg/100g. From this experiments. the weight of seminal vesicles seems to proportional to the concentration of fructose in semen.
2. Administration of testosterone propionate cause the enlargement of the seminal vesicles and the increase of the concentration of seminal fructose, whereas the prolonged administration of testosterone have and inhibitory action and cause the testicular atrophy.
3. Administration of large quantities of estradiol benzoate have an inhibitory action on seminal secretion and cause the testicular atrophy, the decrease of the concentration of seminal fructose and the weight of testis.
4. Follicle stimulating hormones have stimulating action on testis and cause indirectly the increase of the concentration of seminal fructose.
5. The effects of castration on seminal fructose and the effects of testosterone on the appearance of fructose in castrated rats were significant.

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Consecutively from the first report, observations were made on venereal diseases, syphilis, gonorrhea, ulcus molle and lymphogranulomatosis venereum at Okayama Health Centre in 1955 and 1956.
Syphilis gradually decrease since few years, whereas any decline could be found on gonorrhea and ulcus molle, and rather more acute gonorrhea have an increasing tendency.

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This paper is presented the vicissitude of the venereal diseases, syphilis, gonorrhea, ulcus molle, ulcus mixtum and lymphogrannlomatosis venereum in past eight years from 1949 to 1956.
In general, syphilis and gonorrhea have been decreased after the war, especially syphilis is marked and no patients visited in 1955 and 1956.
This shows the influence of the effective use of the antibiotics, but seems to be obscured the latent type.

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The auther, having examined the frequency of ABO-type blood-types that appears in the total 10, 767 inhabitants, containing (including) the eastern shoremen of Koshikijima (male, 4.175, female, 3.738), the western-shoremen (male 726, female, 695) of the same, as well as the villagers of Kashima (male 671, female 762), with similar method as used in the case that examined the fingerprint, has arrived at results as follows:
1. Comparison in frequency proved among the eastern-shoremen, the western-shoremen, both of Koshikijima, and the village people of Kashima.
The east-shoremen and Kashima people proved superior to the west-shoremen in their frequency of O-type blood, and besides, the former proved higher in ratio than Kashima villagers. As to A-type frequency, both the east-and west-shoremen surpassed Kashima villagers, but while in the former no marked difference has been detected among the three, the frequency proved rather high in general; in the frequency of AB-type, the west-coasters proved highest, compared to the east-shoremen or Kashima-villagers, the last proving very low indeed.
II. Comparison of those people of Koshikijima, as well as of Kashima, with several local peoples of Kyushu.
In their high ratio of frequency in O-type blood, the east-shoremen seem to belong the local people of Southern Kyushu; esp., they closely resemble the people of Tanegashima; whereas, the Kashima-villagers enjoy a still higher ratio. The west-shoremen closely approach to the local people of Northern Kyushu, but prove lower in ratio. In A-type frequency, both the east and west-shoremen indicate no special diversity, compared to the Noerthern and Southern Kyushu men; only the Kashima villagers have proved as inferior. In B-type frequency, the former three groups alike stand higher than the South-Kyushu men. In AB-type frequency, the western-shoremen is superior, but in Kashima villagers it has proved lower than local peoples of Kyushu.
III. In eastern as well as western shoremen and Kashima villagers, the frequency of O-type which often characterized the southern peoples, shows more outstanding than in the South-Kyushu men; the eastern shoremen approach close to peoples of South-Kyushu and Tanegashima; moreover, have tendency to approach to such races and tribes as Amami-Oshima, Loochou, Taiwan-Takasago, Micronesia and Indonesia, with gradual stages in crease; the frequency in Kashima people so closely resembles those of Paiwan triwan tribe of Taiwan-Takasago race or Indonesians of Java, proving remarkably southern. In this point, the eastpeople and Kashima people, as seen from the fingerprint, reveal their southern quality; while west-shoremen, similar to the case of fingerprint, approaches to the Koreans in their low frequency of O-type blood type.

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By adding various substrates such as glucose, lactate, pyruvate, aspartate, malate, and alanine to both acatalasemia patients and the normal, the fluctuation of the pyruvic acid contents accompanying the respiration of erythrocytes had been investigated, but no difference of the contents could be observed between the two groups. Thus it has now become clear that even in these patients the pyruvic acid in erythrocytes, in so far as the respiration of erythrocytes, takes hardly any acitve part in the disposal of H₂O₂supposedly to be liberated during respiration.

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By examining the influences of protoporphyrin and of four kinds of metal ions, namely, those of iron, zinc, magnesium, and manganese, and the influences of glucose, lactate, glycerophosphate, acetate, pyruvate, glutamate, succinate, fumarate, malate, and aspartate, methylene blue and DPN, on the oxygen consumption of erythrocytes both in acatalasemia patients and the normal, and by further spectroscopic studies on the changes of the hemoglobin accompanying the respiration of erythrocytes when malate and aspartate of these had been used as substrates, the author reached the following conclusions: namely, in the respiration of erythrocytes of these patients as compared with the normal, no marked difference other than a comparatively weaker enzymatic action of malic dehydrogenase and the enzymes concerning aspartic acid oxidation can be recognized; and as for the disposal of small amounts of H₂O₂ supposedly to be liberated during respiration of erythrocytes, the compensation by peroxidase, hemoglobin, or other hemin proteins may be thought to be sufficient.

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With the purpose to determine whether there will be a catalase diminishing action in the blood of acatalasemic patients, at first the catalase quotients of mixed blood consisting of six sets of two normal persons each selected at random have been computed; and then the catalase quotients of the mixture of the blood of the normal and that of acatalasemic patients have been determined.
As the result each of these two quotients, namely, that of mixture of the normal and that of mixture the normal and patient's, yielded the value equal to the sum of two respective catalase quotients. Therefore, in the blood of acatalasemic patients there seems to be no catalase diminishing action.

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Experimental Method:
1) Reagents: Colloidal fluids used were following:
1. {
These reagents were mixed in a temperature below 85°C. Just before the experiment, 2 and 3 are added to 1.
2. Polymer 120, 0 g.
3. Dibuthyl Phthalate 1, 0 g.
2) How to make moulds:
1. Resin was directly poured into lungs, after cutting off bronchus, under vocal chords, without removing lungs. Hydrochloric acid and water were used as the postmanagement, which removed putride tissues.
2. Total quantity of resin colloids were 2.5-8.0 c. c. Pouring pressure was below 100 mm H₂O.
Results:
6.732 bronchial anfles were observed:
1. Raminification angles of bronchus enlarged in the portion of maturity of newborns, depth and length of neonatal breath. This phenomen appeared frequently in angles of external stratum.
2. In ramification type, right upper lobe chiefly showed two ramifications.

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1. According to the author's data by means of precipitin reactions the fetal blood sera contained a specific protein fraction which did not exist in the adult blood sera, and the same fraction existed also in placenta, amnion water and in the blood sera of pregnant women. These fractions coincided with each other.
2. Some of the blood sera of pregnant women of the 2nd-10th pregnant month gave precipitin reactions against to the fetal blood sera, especially the reactions were more and stronger in those of the 2nd-4th pregnant month, and that it was severer in case of pregnant toxemia, and moreover was the most manifest in case of the mother and child relations.
3. These facts were confirmed also from the side of passive anaphylactic phenomen.

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The authors have examined the ocular fundus of newborn infants (130 cases in total) born at full term in the Obstetrical Department of Okayama University Hospital, by the use of virtual image method, and obtained the following results.
Retinal hemorrhages were found in 30 out of 130 cases (23.1%) and showed a tendency to increase in number in the cases with complications on delivery. It has been thought that the hemorrhage was caused mainly by the congestion and elevation of the intracracranial pressure. The incidence was also higher in toxemias of pregnancy and was 41.7% in the cases born with asphyxia.
In general, the retina was bright and appeared more or less pinkish in color in large number of cases. The margins of papillary discs blurred in 37 cases (28.5%), in which the retinal hemorrhages were greater in number than the average. There were found 16 cases of the coni and one case of pigroid fundus.

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It is very difficulty to prove that a weapon bas been used for the production of a wound generally, and we are observed three wounds, one punctured wound by an instrument, other cutting by a saw in the head and another stab wound of chest by a shape weapon.

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As the factors influencing the neutralization test of rickettsiae, the following ones have been given; mouse organs employed, unequal distribution of rickettsiae in organs and determination method of the result.
The author studied the influences of these factors and other various conditions on the neutralization test. Mitani strain of Rickttsia tsutsugamushi isolated from the patients of Umayado disease in Kagawa prefecture was used as the test agent. The results were as follows:
1) In the present experiment, though some errors should be taken into consideration so far as animal experiment is concerned, it was concluded that reliable result would be obtained by careful selection of the outer conditions and inner factors.
2) As the outer conditions, the experimental temperature and the solution for dilution of rickettsial emulsion are given. The temperature under 10°C is suitable for treatment of R. tsutsugamushi, and glutamine-sucrose-phosphate buffer is superior as the dilution solution.
3) As the inner factors, those of rickettsia itself and those of immune sera were considered. In orser to make the distribution of rickettsiae equal, many livers and spleens were pooled and treated at the low temperature mentioned above. The selection of immune sera is important, and the neutralization test with rabbit immune sera showed the best result.
4) After some observation period of neutralization test, a few of the survived mice showed the syndrom of the disease. This result suggested that prolongation of observation period was necessary to exact determination of the result of neutralization test.

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The author reinvestigated the procedures of neutralization test of Rickettsia tsutsugamushi in regard to the contact period of the agent with immune sera. At the same time, the “Absaettigungsversuch” (Wildfuehr) was also tried on R. tsutsugamushi. Mitani strain of R. tsutsugamushi isolated from the patients of Umayado disease in Kagawa prefecture was used as the test agent. The results were as follows:
1) In test tubes, R. tsutsugamushi combines with its antisera in a short time, and the virulence of R. tsutsugamushi falled along with prolongation of contact period. These facts suggest that estimation of the true titer of neutralization antibody become possible by the short-period-contact which causes no noticeable fall of rickettsial virulence.
2) In the neutralization test of R. tsutsugamushi, dilution of antisera to be mixed with rickettsiae in test tubes deteriorates its result, and addition of human or guinea-pig sera had no influence on its neutralizing effect.
3) No noticeable result was obtained in application of the “Absaettigungsversuch” to R. tsutsugamushi.

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Concerning the S-R dissociation of Sh. sonnei there are abundant reports. The author obtained the following results by employing the virulent strain which had caused violent food poisoning.
At first in order to facilitate the differntiation of S-R changes the Endo culture medium was partially modified and the variation of Sh. sonnei has been observed by performing the series of cultures on the medium taking serological aspects into consideration.
1) With the S-type the series of cultures successive incubations were carried out for 300 generations and always about five per cent of them indicated R-type dissociation.
2) In the series of cultures on the medium loaded with certain sugars the R-type dissociation became less apparent and after the fifth in every generation a wholly pure S-type was obtained.
3) In the experiments comparing the virulency of the bacteria cultured under the various conditions against animals, the virulency of the bacteria cultured in the soft gruel of rice malt proved to be strongest.
4) In the experiment of reconversion of the R-type bacteria to the S-type, either by culture in the media loaded with various substances or by passage through animals, no reconversion of R-type back to S-type has taken place at least in the author's experiments.

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There are just as many reports on the l-histidine decarboxylase activity of dysentery bacilli as those on the other bacteria.
In the course of elucidation of the causative factors of violent food poisoning that occurred in the Okayama Prefecture in 1953, the author has made a report on the S-R dissociation and the virulency of Sh. sonnei in the Report 1. In the preaent report the biological nature of the virulent strain is described.
1) Sh. sonnei grow most rapidly in the medium whose optimal pH is 7.0-7.6, but when the pH falls under 4.3 the growth of bacteris is completely inhibited: and in addition, the peaks of the growth are correlated with changes in the pH of the medium.
2) In the cultures performed in test-tubes and flasks, R-type bacteia grew in the flask much better than S-type, while on the contrary in the test-tubes R-type bacteria growth was inferior to that of the S-type.
3) As for the growth of Sh. sonnei in soft gruel of rice malt, although the growth does not reach as high degree as observed in the peptone medium but it reaches the maximum growth within 12 hours. After 24-hour culture, however, the growth tends to decline.
4) On examining by paper chromatography and by Warburg's apparatus the l-histidine decarboxylase activity of the isolated strain that has been cultured in the media of peptone solution loaded with l-histidine and with additional various sugars, the excretion of carbon dioxide gas and the production of histamine are observable only in the cases where these bacteria are grown in the media containing additional glucose, maltose, or mannose.
5) Acetone powder of Sh. sonnei that has been cultured in the medium of glucose-peptone solution loaded with l-histidine possesses the l-histidine decarboxylase activity and acts specifically to the substrate.

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In order to reinvestigate the natures of hepatitis virus in detail, the author studied the general natures of hepatitis virus in comparison with ectromelia virus. The results are summarized as follows:
1) Though hepatitis virus and ectromelia virus are of great similarity in their various natures, collective consideration of the heat-resistance, the drug-resistance. the hemoagglutination reaction etc. makes possible the differentiation between these two sorts of viruses.
2) In the serial passage in mice, though the mixed infection of ectromelia virus should be considered, the discrimination between these two sorts of viruses is possible by observation of infectious aspects and general natures.

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As for the immunological natures, particularly for the neutralization test and the “Absaettigungsversuch”, the isolated hepatitis virus was compared with analogous viruses. The results are as follows:
1) Both of the neutralization test and the “Absaettigungsversuch” of ectromelia virus are ef practical use ot least to a limitted degree. The “Absaettigungsversuch” is, however, of less use than the neutralization test, and is inferred to give a definite result only in combination with the neutralization test. Consequently, combined employment of the both tests is desirable.
2) Hepatitis antisera showed a high neutralization with hepatitis virus, and not with the other viruses. The result of this neutralization test was determined by observation of pathological findings.
3) In short, the results of these immunological tests showed that the viruses of infectious hepatitis, ectromelia and “Izumi fever” were clearly different to each other, and distinct discrimination was possible among them.

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In order to discriminate the isolated infectious hepatitis virus from mouse hepatitis virus (Buscher strain), of which both are very similar in their various natures, the author carried out various experiments and obtained the following results:
1) Only the suckling mouse is susceptible to mouse hepatitis virus and succumbs to its infection, whereas it is rarely killed by the isolated hepatitis virus.
2) The chick embryo is little susceptible to mouse hepatitis virus, but is highly susceptible to hepatitis virus and is killed in a few days.
3) Whichever of the suckling mouse and chick embryo may be used as the test animals, and whichsoever of both viruses as the interfering or challenging virus, no interfering phenomenon can be observed between mouse hepatitis virus and the isolated infectious hepatitis virus.
4) In the immunological test, “Absaettigungsversuch”, no relation can be observed between these two viruses.
All of these results suggest that mouse hepatitis virus and the isolated infectious hepatitis virus are clearly different to each other.

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Ammonia of the rat brain in case of the injection of chlorpromazine was measured by Conway's microdiffusion method, and amino acid by the paperchromatography, and the following results have been obtained in the respective measurements.
1) Following the injection of chlorpromazine (50 mg/kg per once), the ammonia contents of the rat brain decreased to 0.34 mg. per cent.
2) In case of repeated injection of chlorpromazine (20 mg/kg every day), the ammonia contents of rat brain increased to 0.93 mg per cent, and the simultaneous decrease of glutamic acid was not found.
3) The amounts of ammonia formed by above mentioned brain slices were found to be 6.2 μM/g. for the first one hour, 7.1 μM/g in two hours, 9.3 μM/g. in three hours and 12.7 μM/g in four hours, respectively. Namely, in this case, the increase of ammonia formation was found during the first one hour and the decrease of it was found thereafter.

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Thirty-three patients with anemia and hypoproteinemia were anesthetized with cyclopropane and the changes in the peripheral blood corpuscles and the plasma protein were observed before and after anesthesia.
1) The number of the erythrocytes slightly increased already 20 minutes after starting anesthesia, while the leucocytes markedly increased. In the increase of the leucocytes, An increase of the neutrophils and an marked increase of the lymphocyte were observed. Hemoglobin as well as hematcrit increased, while the water content of blood and plasma decreased.
2) Total plasma protein increased and its fractioning revealed an increase of A/G ratio, slight increase of albumin, decrease of α-globulin as well as φ, slight increase of γ-grobulin and no change of β-globulin in percentage. From the quantitative point, there were observed an increase of Al. slight increase of β-Gl., decrease of φ, increase of γ-Gl. and no change of α-Gl..
3) These changes in the peripheral blood corpuscles and the plasma protein are considered to be due to the temporary increase of adrenalin secretion caused by cyclopropane as stressor, though there may be some exemia (concentration of the blood) due to general anesthesia.

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Twenty-two patients with anemia and hypoproteinemia were anesthetized with cyclopropane and the interrelationships between the induction time of anesthesia and the peripheral blood corpuscles or plasma protein were investigated.
1) A reverse interrelationship was seen between the induction time of cyclopropane anesthesia and the water content of the plasma.
2) A parallel interrelationship was seen between the induction time of cyclopropane anesthesia and the total plasma protein.
3) The interrelationships were not found between the induction time of cyclopropane anesthesia and hemoglobin, the erythrocytes, the increased rate of the leucocytes, hemoglobin concentration, hematcrit, the water content of blood and each fraction of the plasmaprotein.
4) In the patients with hypoproteinemia, the stage of anesthesia easily tends to be deeper. Therefore, coutious attention should be called.
5) On the contrary, in the patients with anemia there were not the tendency to be deeper. I imagine cyclopropane is well available for the patients with anemia.

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In the age when there are so many users of narcotic drugs as at the present, it is rather surprising that nothing definite is known about the concentration of barbital in blood after its administration; and this can be keenly realized if one takes into consideration the fact that barbital is one of the most frequently used narcotic drugs. This aroused my interest in intiating the study on the barbital concentration in body. I have examined human body repeatedly with the use of Goldschmidt's method and measured the barbital concentration in blood. As the results, it has been found that the barbital concentration in blood reaches its maximum about two hours after its administration, and although it gradually decreases thereafter, it still maintains a relatively high level after twenty-four hours. This point coincides well with that of drugs clinically used for inducing lasting sleep.

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The pathway by which barbital reaches the brain is still unknown. Generally there are two possible pathways through which substances in blood enter into the brain, namely, one, directly through blood vessels and the other, by way of the choriomeningeal plexus. Barbital will possibly enter the brain by the former pathway but it may enter the brain by way of the cerebro-spinal fluid as in the case of P.³² In order to verify this point, I have measured the barbital concentrations in the lumbar fluid and the ventricle fluid, each obtained at the time of lobotomy and at the pneumo-ventriclography, respectively.
From these results, it has been found that barbital immigrated into the cerebro-spinal fluid faster than bromine so that there seems to be a possible pathway to the brain through this fluid. Moreover, in the measurements of the barbital concentration in the ventricle fluid after oral administration has been found higher than that in the lumbar fluid.
For this reason, I believe that the choriomeningeal plexus plays the leading rôle in the production of the cerebro-spinal fluid as contended by other investigators.

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As described in report 1, I obtained a vague idea about the relationship between the barbital concentration in blood and sleep in my previous experiments. In order to clarify this relationship still further, I used the δ-index of E. E. G. for the numerical representation of depth of sleep, and examined the possibility of a parallel relationship between this index and the barbital concentration in blood.
The results revealed that ths two fluctuate in parallel up to one to two hours after oral administration of barbital but beyond two hours the δ-index decreased rather independently of the barbital concentration in blood. From this result, the brain seems to posess a resistant (a familiarized phenomenon) at this stage to barlital, and therefore, the beginning of a habitual tendency had apparently been created in the brain.

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The author studied on the pathogenicity and complement-fixing antigenicity of the peritoneal exudate of guinea-pigs previously injected with chlorpromazine and then inoculated with Tanizawa strain of Rickettsia tsutsugamushi. Both of the exudates with and without visible rickettsiae were used as the experimental materials. The results were as follows:
1) whether contained visible rickettsiae or not, the exudates showing the specific inflammatory pictures had no significant difference in their pathogenicity.
2) The exudates showing the specific inflammatory pictures had the same titer of complement-fixing antigenicity, regardless of appearance or non-appearance of visible rickettsiae.
3) Whether contained visible rickettsiae or not, the exudates showing the specific inflammatory pictures did not show any significant difference of antigenicity against the rabbit immunesera immunized by subcutaneous, intraperitoneal or intrascrotal inoculation of exudates. Of all the inoculation sites tested, intraperitoneal one produced the antisera of the highest titer.
4) In short, regardless of the presence or absence of visible rickettsiae, the peritoneal exudates showing the specific inflammatory pictures had no noticeable difference in their pathogenicity and complement-fixing antigenicity. The cellular pictures of peritoneal exudates are the specific reaction figures to multiplication of rickettsiae, and appearance of rickettsiae depends on the growing stadium of ricketsiae and, moreover, depends on the technic of stain.

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By intraperitoneal or intranasal inoculation of testical or cerebral emulsion of guinea-pigs infected with Rickettsia mooseri or R. prowazeki of mice and the cellular pictures of peritoneal exudate. The results were as follows:
1) By intraperitoneal inoculation, R. mooseri could produce manifest infection in all the seasons but that of high temperature, while R. prowazeki could not in any season.
2) By intranasal inoculation, both rickettsiae could cause the sickness in all the seasons except that of high temperature. R. mooseri, however, caused severer attack than R. prowazeki.
3) Even by intraperitoneal inoculation of R. mooseri of non-manifest infection, the serial passage of the rickettsia was well carried out.
4) After intraperitoneal inoculation of R. mooseri into mice in April, fibrocytes increased in the peritoneal exudate on the 1st to 4th day, fibrohistiocytes on th 5th to 7th day, and histiocytes on the 8th to 10th day.
5) In hunger-breeding mice previously injected with benzene and olive oil, intraperitoneal inoculation of R. prowazeki did not cause any manifest attack.

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