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Volume 70, Issue 9
Displaying 1-11 of 11 articles from this issue
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Atsuo IWASAWA, Yoshiko NAKAMURA1996 Volume 70 Issue 9 Pages 915-922
Published: September 20, 1996
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSAcidic electrolyzed water is made recently by various kinds of machines and is widely utilized. In this study, we intended to clarify the relationship between the concentration of chloride and pH in the bactericidal effects with acidic electrolyzed water. The effects of weak or strong acidic electrolyzed water were compared with a pseudo-acidic water of pH adjusted by diluted hydrochloric acid and sodium hydroxide, on Staphylococcus aureus, Staphylococcus epidermidis and Pseudomononas aeruginosa. At pH 5.0-6.0, 3 bacterial strains were killed soon after being exposed to the acidic water containing chloride 50 mg/liter, and the amount of chloride did not change after allowing to stand open for 6 hours. At pH 2.67-2.80, the bactericidal effects was observed at the concentration of chloride 5 mg/liter, and 80% of chloride remained after allowing to stand for 6 hours. These results indicated that newly made strong acidic water is more effective under a smaller amount of chloride at pH 2.7, and that weak acidic electrolyzed water should be used, if stable bactericidal effect is expected in cleaning the surroundings.View full abstractDownload PDF (871K) -
Junichi HONDA, Yasumitsu OKUBO, Keisuke ARIKAWA, Mutsuko KUMAGAI, Mika ...1996 Volume 70 Issue 9 Pages 923-930
Published: September 20, 1996
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSProphlaxis of CMV-associated disease and/or early initiation of therapy is important in the management of patients with CMV infection. Recently, we developed the CMV antigenemia assay: a rapid and quantitative method based on the detection of CMC antigens in peripheral blood leukocytes by flow cytometry. Heparinized peripheral blood was obtained from healthy donors, bone marrow transplantation patients, oatient with collagen disease and patietns with adult T cell leukemia. To determine the phenotype of HCMV-infected mononuclear cells, the following phycoery thrin-conjugated mAb were used: CD8, CD15. These mAds were added to the whole blood and incubated. After the hemolysis, the cells were fixed with 4% paraformaldehyde and 0.3% NP-40. To determine the HCMV-infected cells, the following mAb were used in flow cytometry analysis: E13 (Chemicon Inc., Toyo) against HCMV IEA. As a secondary antibody, a FITC-conjugated goat anti-mouse IgG. In the bone marrow transplant patients, CMV-associated antigen was positive in their monocytes and polymorphocytes. In the patient with collagen disease, CMV-antigen was positive in their lymphocytes and monocytes.
Our study demonstrates that the detection of CMV antigen-positive blood leukocytes by FACScan is a rapid and quantitative method and useful for the diagnosis and monitoring of CMV-associsated CMV-associated disease. The CMV blood antigen assay by FACS can will facilitate the initiation of early treatment with ganciclovir of CMV-associated disease or the administration of prophylactic ganciclovir for preventing the disease.View full abstractDownload PDF (922K) -
Shunji MATSUDA, Masahiro NODA, Akie TOYOTA, Shizuyo TOKUMOTO, Masahiko ...1996 Volume 70 Issue 9 Pages 931-937
Published: September 20, 1996
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSIsolation of HIV-1 was tried in PBMCs separated from 27 blood samples which were sent to Hiroshima Prefectural Institute for Health & Environment Science for anti-HIV antibody-test. 18 samples were positive for HIV-isolation (4 samples were Rapid/High type, 12 samples were Slow/Low type and 2 samples were intermediate). Compared with clinical stages of patients, samples from patients in CDC-II were 64%(14/22) positive and those in CDC-IV were 100%(3/3) positive in HIV-isolation. Improvement of the methods is tobe required to isolate HIV from the samples of patient in the latent phase. Removal of CD8 positive cells from PBMCs of patients did not affect the efficiency of HIV-isolation by our methods. Three parts in amino acid sequence of V3 region in proviruses were different between Rapid/High type isolated and Slow/Low type isolates. The difference of amino acid may be related to the different phenotypes of the two viruses.View full abstractDownload PDF (2587K) -
Fumiaki TAGUCHI, Shunji OKUDA, Utsuki UCHINO, Hiroe MURAOKA, Miyuki HA ...1996 Volume 70 Issue 9 Pages 938-946
Published: September 20, 1996
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSPMAC agar, a novel, selective and differential medium has been developed and was subjected for evaluation of its selective and differential capability of methicillin resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa from other bacteria such as Bacillus, Micrococcus, Gram-negative bacteria and drug resistant ones. Growth of MRSA and P. aeruginosa on PMAC agar was facilitated and their colonies were easily differentiated. Colonies of MRSA after 24-48h incubation at 35°C were small (2 to 4 mm in diameter), smooth and egg-yolk reaction positive. On the other hand, P. aeruginosa with pigment productiuon (pyocianin, fluorescin or pyomelanin) formed large (2.5 to 7.0 mm in diameter), brownish black or brown colonies with a creamy edge. PMAC agar did not allow to grow unwanted bacteria tested except certain species formerly classified to Pseudomonas such as Burkholderia and Stenotrophomonas. However multi-drug resistant strains such as Enterobacter cloacae, Serratia marcescens and Acinetobacter calcoaceticus formed extremely small colonies. PMAC agar is recommended as a novel, useful medium for isolation, differentiation and presumptive identification of MRSA and P. aeruginosa from clinical and environmental sources.View full abstractDownload PDF (3463K) -
Masayuki TSUJIMOTO, Masayoshi SAWAKI, Keiichi MIKASA, Mitsuru KONISHI, ...1996 Volume 70 Issue 9 Pages 947-954
Published: September 20, 1996
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSWe have performed a clinical study on chronic lower respiratory tract infection (CLRTI) with Haemophilus influenzae (H. influenzae) by transtracheal aspiration (TTA) and analized clinical factors of the acute exacerbation.
In 40 episodes (38 cases) of H. influenzae isolated from CLRTI, monobacterial infection with H. influenzae were 21 episodes and polymicrobial infection were 19 episodes. We classified the disease into acute exacerbated (27 episodes) and stable (13 episodes) phase and the former episodes were divided into bronchitis type (7 episodes) and pneumonia type (20 episodes). Polymicrobial infections were seen more in the pneumonia type (13 episodes) than in the bronchitis type (2 episodes). The principal organism detected with H. influenzae were α-Streptococcus and Neisseria sp. in the bronchitis type and S. pneumoniae in the pneumonia type.
The acute exacerbated cases were divided into the following 4 patterns; 1. polymicrobial infection with continuous infection of P. aeruginosa, 2. monomicrobial infection after acute upper respiratory tract infection, 3. polymicrobial infection with S. pneumoniae after continuous infection of H. influenzae, 4. bacterial replacement by P. aeruginosa after acute exacerbation.
The results of the study suggests that polymicrobial infection is an important chronic lower respiratory tract infection when caused H. influenzae.View full abstractDownload PDF (931K) -
Naoshi YANAGISAWA, Masamitsu TAKAHASHI, Masahiko NAMIKI, Shigetaka OKA ...1996 Volume 70 Issue 9 Pages 955-962
Published: September 20, 1996
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSWe studied the erythrocyte sedimentation rate (ERS) and related factors that influence ESR in 326 patients with Pulmomary tuberculosis. The ESR tended to be higher for aged subjects and showed a difference between patients under 59 years old and patients over 60 years old. Besides, patients under 30 years old tended to present lower ESR. It is suggested that they may be under the influence of inapparent infection. For The Japanese Society for Tuberculosis Classification, a significant difference was noted in the extent, but there was no difference about the presence of cavity. A significant correlation was detected between ESE and Gaffky scale, ESR and CRP, and ESR and period until sputum culture became negative. But CRP negative cases were observed in about 28%. CRP alone was not sufficient for the evaluation of the morbid condition of Mycobacterium infection. Receiver-Operator Characteristic curve analysis confirmed that ESR is useful among patients with pulmonary tuberculosis. Therefore evaluation of ESR together with CRP was needed.View full abstractDownload PDF (772K) -
Masamitsu NAKAJIMA, Yoshihito NIKI, Toshiaki MANABE, Toshiharu MATSUSH ...1996 Volume 70 Issue 9 Pages 963-969
Published: September 20, 1996
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSThe usefulness of the serial section method was investigated in four bone marrow aspirates, four biopsies and nine autopsy specimens obtained from patients with miliary tuberculosis who were admitted to our hospital between 1974 and 1994. In original sections, granulomas consistent with tuberculosis were found in two out of four aspirate specimens (50%) but in none of the four bone marrow biopsies. By using serially out sections, however, granulomas were detected in two more aspirate specimens and two biopsies which were negative in original sections. Consequently four of four aspirates (100%) and two of four biopsies (50%) became positive. In addition, the distribution of granulomas was investigated using vertebral bone marrows obtained from 12 autopsy cases. We divided the vertebral bone marrows obtained from 12 autopsy cases. We divided the vertebra into the peripheral and central portions. Granulomas were more frequently found in the peripheral portions than in the central.
The higher detection rate of granulomas in the peripheral bone marrow when the biopsy and aspiration needles easily reach, suggests that bone marrow aspiration and/or biopsy would be a useful procedure for the diagnosis miliary tuberculosis. Therefore if serial sections were utilized for such samples in the miliary tuberculosis patients, the detection rate is expected to became much higher.View full abstractDownload PDF (5891K) -
Manabu KUROKAWA, Masafumi NUKINA, Hisao NAKANISHI, Kanji MIKI, Syusuke ...1996 Volume 70 Issue 9 Pages 970-975
Published: September 20, 1996
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSHemorrhagic gastric ulcer were often found in the population who suffered from the Great Hanshin-Awaji earthquake. Gastric biopsy samples taken from 136 patients in the population were examined for Helicobacter pylori by culture and PCR methods. Furthermore, production of CagA protein from isolates was investigated. Number of positive cases with culture and PCR method were 20 (50.0%) and 26 (65.0%) in hemorrhagic ulcer, 62 (86.1%) and 65 (90.3%) in other ulcers, respectively. Number of CagA positive strains from the patietns of the hemorrhagic ulcer and other ulcers were 20 (76.9%) and 21 (32.3%), respectively. The significance in both ulcers was observed (pv0.001). From the present results, it was considered that the stress of the earthquake, the infection of H. pylori and the production of CagA protein of the organism were combined and this caused hemorrhagic gastric ulcers.View full abstractDownload PDF (4424K) -
Yoshina SHIMADA, Yasuhiro YAGI, Yumiko HIRAGA, Shoji KAWANO, Kihei TER ...1996 Volume 70 Issue 9 Pages 976-980
Published: September 20, 1996
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSWe reported a case of 10-year-old girl with petechiae mainly on the truncus, although who had more than 10×104/μl of platelets. Smears of her bone marrow demonstrated extreme reduction of erythroblasts with giant proerythroblast like cells, which does not consist with idiopathic thrombocytopenic purpura. Human parvovirus B19 (B19) DNA was detected in her serum and throat swab using PCR and significant alterations of B19 specific IgM and IgG antibody titers were demonstrated. The petechiae subsided on the 8th day after her admission. She had not shown anemia less than Hb 10g/dl, but her reticulocytes counts had increased up to 3.5% on the 11th day after her admission. Smears of her bone marrow on the 17th day became normal with moderate erythroblasts.
It is demonstrated that the cellular receptor of this virus is an antigen of the blood group P recently. P antigen is presented not only on erythrocytes and on erythroblasts but also on megakaryocytes and endotherial cells. Therefore, it is suggested that direct injury by B19 to endothelial cells could cause petechiae in this case.View full abstractDownload PDF (2922K) -
Shuji SAITO, Takako DOI, Shingo FUKAZAWA, George WADA, Jun-ichi WAKASU ...1996 Volume 70 Issue 9 Pages 981-985
Published: September 20, 1996
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSA 62-year-old female patient was given cancer chemotherapy for lymph nodes metastases in the left breast cancer. She was admitted to the hospital because of severe watery diarrhea, in hypovolemic shock, and was diagnosed as suffering from not-typhoidal Salmonella by stool culture. After systemic administration of antibiotic agents, she became well in a few days, but on the 16th hospital day, she had severe watery diarrhea, hypovolemic shock and then cardiac arrest. She was resuscitated immediately. The stool culture revealed methicillin-resistant Staphylococcus aureus (MRSA), type II coagulase, producing TSST-1 and type BC staphylococcal enterotoxin.
It was thought that in this case, MRSA enteritis was caused by damage of the intestinal mucosal barrier of the defense mechanism against infection due to salmonellosis and administration of multiple antibiotic agents.View full abstractDownload PDF (560K) -
1996 Volume 70 Issue 9 Pages 1049
Published: 1996
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSDownload PDF (40K)
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